7. PRINCIPAL---
1.Enzyme are biocatalyst nd catalyze the biochemical
reaction.So defficiency cause the metabolic reaction
eg lactose intolerence
2.BASIC PRINCIPAL:
Every enzyme has got the tissue specificity nd
intracelluiar locational specificity to catagorically
indicate the organ involved in disease process
3.Enzymes activity can be inhibited reversibly nd
irreversibly so this can be used for clinical application
8. 4 .Enzyme can be produced in laboratory by
bioenginering nd biotechnology .
5.Plasma contain functional nd non functional
nd non funtional plasma enzyme.
ENZYME IN BLOOD
a)Functional
b) Nonfunctional
c) common criteria
9. a) Fuctional:They have got the function in blood
Concentration more in blood than plasma
e,g Lipoprotein lipase(LPL)
clotting factors
b)Non functional:No function in plasma.
concentration more in cell than plasma
e,g SGPT,SGOT,LDH,CPK
c)Common criteria:functionaly nd non fuctionaly they
form nd come to plasma
e,g cerulopasmin(transport protein for iron)S
10.
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12. USE OF ENZYME IN CLINICAL FIELD
1)Utiological study of disease.
2) Enzyme are used as diagnostic tool of disease.
3) Enzyme are used as prognostic tool of disease.
4) enzyme are used as therapeutic tool of disease.
5)Enzyme inhibition used as therapeutic tool of
disease.
6)Use of enzyme as laboratory tool for diagonosis
of genetic disease.
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26. TYPES OF ISOENZYME :
Compositional point of view
1)True Isoenzyme: produce by different gene from
different locus
e,g salivery amylase, pancreatic amylase
lingual lipase, pancreatic lipase
2) Allo-Isozyme: different Allele in same locus
e,g G6PD -400---500 varieties(one variety
present in one individual)
3)Hybrid Isozyme: produce multiple molecular form by
different subunits in various combination
CPK 1,2,3,4 LDH 1,2,3,4,5
27. * True Isozyme nd Allo-Isozyme:
Both monomeric protein single polypeptide chain
differing a respect of few AA
Alkaline phosphatase
ALP – placenta
bone
liver
Carbomoyl Phosphate synthase I,II
I in mitochondria
II in cytoplasm
Hybrid isozyme oligomeric protein
