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1. ARBAMINCH COLLEDE OF HEALTH SCINCE
DEPARTEMENT OF MEDICAL LABORATORY
ASSIGNIMENT OF MOLOCULAR BIOLOGY FOR GENERIC BSC LAB STUDENTS
NAME TAMRAT TESFAYE IDNO BscpLab 48/13
1. Write at least three features that make DNA to be the genetic material? 2 pt
A) Inheritance
B) Gene Expression (translation and transduction )
C) Store genetic information
2. Compare and contrast DNA and RNA in terms of nucleotides, structure /number of stranded , role played
inside cell and site where it works 2 pt
Nucleic acid Types of nucleotides Number of
strand
Major role Place of
work
DNA
:-Deoxyadenosine 5'- monophosphate
:-Deoxyguanosine 5'- monophosphate
:-Deoxycytidine 5'- monophosphate
:-Deoxythymidin 5'- monophosphate
-Double-
stranded
-inheritance
-store genetic
information
Nucleus
RNA :-Adenosine 5'- monophosphate
:-Guanosine 5'- monophosphate
:-Cytidine 5'- monophosphate
:-Uridine 5'- monophosphate
-single-
stranded
-protin synthesis Cytoplasm
3. Combination of a sugar with a base occurs by condensation rxn in DNA or RNA form nucloside while
Nucleotides refers combination of phentose sugar,N.base and phosphate group2pt
4. Writ at least three physical factors that denature DNA molecules and alter Biochemical synthesis inside
cell. 2pt
* PH
* Heat
* Free radical
5. Why we call DNA replication is said to be semi conservative rather than conservative? 2pt
* Because, each helix that is created contains one strand from the helix from it was copied
I Okazaki fragments bind with one another
II Nucleotides added one on another to form leading and lagging strand
III. Short segment of RNA synthesized at the 5’end of DNA
6. Based on the above information Match DNA replication phases as parent strand copy itself to two
daughter strands 2 pt
A) Initiation ____lll________ B) Elongation _______ll____ Termination_____l_______

2. 7. Discuss on the function of each of DNA replication enzymes . 2pt
A) Helicase :- unwinds DNA strands
B) Topoisomerase:-keeps DNA ahead of fork from over winding
C) DNA Polymerase III :- catalyses elongation of new DNA strands
D) DNA Polymerase I :- removes the RNA primers and replace it with DNA adjacent to the fragments
E) DNA ligase :- joins 5' phosphate of one DNA molecules to the 3' OH of another
F) primase:-synthesize RNA primers needed to start replication
G) Single strand binding protein:-prevent strands from re-annealing
8. What is the difference b/n replication in prokaryotes and eukaryotes. 2pt
A) Prokaryotes :-replication uses as single origin to rapidly replicate the entire genome
B) Eukaryotes:-DNA replication requires multiple replication forks
9. What does polymerization of nucleotides in DNA occur only in 5’ to 3’ direction mean ? 2pt
** Nucleotides are added only to the 3 end of the growing strand
10. Write the experimental contribution of the following molecular biologist? 2pt
A) Watson Crick :-determined the double-helix structure of DNA
B) Chargaff:-in DNA the ratio of A-T and G-T
C) Miescher:-isoletd "nuclein" DNA with associated proteins from cell nucliec