Extracellular Vesicles (EVs) derived from mesenchymal stem cells are known to have therapeutic potential, with several clinical trials ongoing. EV applications to drug delivery platforms have also been developed. Increasing research efforts in the EV field have yielded an appreciation for the potential role of glycans in EV function. TCI can synthesize various glycans related products to support your research, - For Insertion (chemical synthetic oligosaccharide) - For Detection (antibodies and Lectins) - For Cleavage (Enzymes)

1. Glycan-related Reagents for
Extracellular Vesicle (EV) Research

2. Extracellular Vesicles
• Extracellular Vesicles (EVs) are small vesicles with lipid bilayer that are
secreted from various cells under physiological and pathological
conditions and exist in the body fluids and cell culture supernatant.
• EVs contain nucleic acids and proteins, and act as mediators of
intercellular communication by entering the target cells.
• EVs include subtypes such as exosomes and microvesicles.
• They are involved in many physiological responses and diseases,
including cell differentiation, immune responses, infection, cancer,
cardiovascular diseases, and neurodegeneration.

3. Extracellular Vesicles
• EVs derived from mesenchymal stem cells are known to have
therapeutic potential, with several clinical trials ongoing.
• EV applications to drug delivery platforms have also been developed.

4. Glycans
• Glycans exist not only on the surface of cells, but also on the surface
of sEVs (small Extracellular Vesicles: EVs <200 nm in diameter) as
glycoproteins or glycolipids.
• Importance of glycans on EVs has been shown. For example,
glycocalyx on the cancer cell-derived EVs has been reported to involve
in organotropic determination in cancer metastases.
• Glycans on the EV have been reported to play important role in the
tuning of EV uptake.
TCI has many products related to glycans expressed on the EVs.

5. Glycolipid-related Glycans
• Glycan patterns are well known to change during various biological
events, which include cancer, inflammatory diseases, and immune
diseases, and the same applies to sEV surface glycans.
TCI can synthesize various glycans on the cell surface. We offers you
oligosaccharides which play important roles such as intercellular
recognition, adhesion, proliferation control and signaling.

6. Glycolipids
Products

7. Glycolipids Products
G0509 α-GalactosylCeramide
G0580 Gb4 Ceramide
G0592 Gb5 Ceramide (= SSEA-3)
G0589 Globo-H Ceramide
G0593 SialylGb5 Ceramide (= SSEA-4)
G0483 Ganglioside GM1
G0489 Ganglioside GM3
G0422 Ganglioside GM3 (phyto-type)
G0510 Ganglioside GM3(Neu5Gc) (phyto-type)
S0910 Sialyl Neolactotetraosylceramide (= Sialyl nLc4Cer)

8. Oligosaccharides
Products

9. Oligosaccharides Products
G0479 Globotriose
G0403 Gb3-β-ethylazide
G0402 Gb3-β-ethylamine
G0623 Gb4-PrN3
G0434 Globopentaose
G0599 Gb5-PrN3
G0596 Globo-H-PrN3
G0447 Globo-H-PrNH2
S0946 SSEA-1-PrNH2
S0849 Sialyl Lewis X-Lactose
N1147 Neu5Acα(2-6)Gal-β-propylamine
N0950 Neu5Acα(2-6)Galβ(1-4)GlcNAc-β-ethylamine
N1249 Neu5Acα(2-3)Gal-β-propylazide
N1146 Neu5Acα(2-3)Gal-β-propylamine
N1267 Neu5Acα(2-3)Galβ(1-4)GlcNAc-β-propylamine
N1258 Neu5Acα(2-3)Galβ(1-4)Glc-β-propylamine
N1259 Neu5Gcα(2-3)Galβ(1-4)Glc-β-propylamine

10. Anti-glycolipid Antibodies
• Antibodies are proteins which are one of the components of the
immune system. The specificity of antibodies is likened to the
interaction between a key and a keyhole. Anti-glycolipid antibodies
can specifically recognize glycolipids.
• TCI mainly produce antibodies against glycolipids; Ganglio-series,
Globo-series, Lacto-series, and Neolacto-series.
• These antibodies can be used for immunohistochemistry, cell-
staining, flow cytometry, ELISA, TLC-immunostaining and other
methods.
• Our antibodies are very useful tools for analyzing the expression of
carbohydrate chains and their functions.

11. Products
A2505 Anti-GM1 Monoclonal Antibody
A2576 Anti-GM2 Monoclonal Antibody
A2582 Anti-GM3 Monoclonal Antibody
A2507 Anti-GD1a Monoclonal Antibody
Anti-glycolipid Antibodies

12. Anti-glycolipid Antibodies Products
A2508 Anti-GD1b Monoclonal Antibody
A3338 Anti-GD2 Monoclonal Antibody
A2580 Anti-GD3 Monoclonal Antibody
A2702 Anti-GT1a Monoclonal Antibody
A2732 Anti-GT1b Monoclonal Antibody
A2662 Anti-GQ1b Monoclonal Antibody
A2701 Anti-GalNAc-GD1a Monoclonal Antibody (2A3D2)
A2506 Anti-Gb3 Monoclonal Antibody
A2822 Anti-Gb3 Monoclonal Antibody Biotin Conjugate
A2706 Anti-SGPG (HNK-1) Monoclonal Antibody

13. Lectins
• Lectins are highly specific carbohydrate-binding proteins of
nonimmune origin. Due to their ability to bind with cell-surface
glycoproteins and glycolipids, lectins could agglutinate cells; they also
could reversibly associate polysaccharides and glycoproteins in
solution.
• Lectin has long been well-known as tools for detection and analysis of
functional oligosaccharides in the glycoscience field. Recombinant
lectins show better stability compared with lectins extracted from
natural resources.
• TCI offers not only recombinant lectins but also chemically modified
lectins such as several types of biotinylated lectin and lectin-agarose
for detection and capturing of glycoconjugates.

14. LecBeads (Lectin-Agarose)
Products
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]

15. Lectin-Biotin Conjugates
Products
R0230 rPSL1a-Biotin [for Siaα(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate

16. Lectins
Products
R0225 Recombinant Polyporus squamosus lectin (= rPSL1a) expressed in E. coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in E. coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in E. coli
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in E. coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in E. coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae

17. Endoglycosidases
• Endoglycoceramidase (EGCase) is a glycolipid-specific hydrolase that
cleaves the glycosidic linkage between oligosaccharide and ceramide
of various glycosphingolipids (GSLs).
• Recombinant EGCase (rEGCase) is activated under a detergent-
dependent condition. In contrast, Activator II is capable of inducing
activity of EGCases without any detergent reagents.
• By the use of Activator II, GSLs on cell surfaces of living cells could be
hydrolyzed without cell disruption caused by detergent.

18. Endoglycosidases and Related Enzymes
Products
R0240 rEGCase I
R0241 rEGCase I assisted by Activator II
R0242 rEGCase II
R0243 rEGCase II assisted by Activator II
A1651 Endo-M (= endo-β-N-Acetylglucosaminidase)
G0365 Glycosynthase (= Endo-M-N175Q)
E1339 Endo-M-W251N
A1844 endo-α-N-Acetylgalactosaminidase (=Endo-α)
K0069 Keratanase II