SlideShare a Scribd company logo
Glycan-related Reagents for
Extracellular Vesicle (EV) Research
Extracellular Vesicles
• Extracellular Vesicles (EVs) are small vesicles with lipid bilayer that are
secreted from various cells under physiological and pathological
conditions and exist in the body fluids and cell culture supernatant.
• EVs contain nucleic acids and proteins, and act as mediators of
intercellular communication by entering the target cells.
• EVs include subtypes such as exosomes and microvesicles.
• They are involved in many physiological responses and diseases,
including cell differentiation, immune responses, infection, cancer,
cardiovascular diseases, and neurodegeneration.
Extracellular Vesicles
• EVs derived from mesenchymal stem cells are known to have
therapeutic potential, with several clinical trials ongoing.
• EV applications to drug delivery platforms have also been developed.
Glycans
• Glycans exist not only on the surface of cells, but also on the surface
of sEVs (small Extracellular Vesicles: EVs <200 nm in diameter) as
glycoproteins or glycolipids.
• Importance of glycans on EVs has been shown. For example,
glycocalyx on the cancer cell-derived EVs has been reported to involve
in organotropic determination in cancer metastases.
• Glycans on the EV have been reported to play important role in the
tuning of EV uptake.
TCI has many products related to glycans expressed on the EVs.
Glycolipid-related Glycans
• Glycan patterns are well known to change during various biological
events, which include cancer, inflammatory diseases, and immune
diseases, and the same applies to sEV surface glycans.
TCI can synthesize various glycans on the cell surface. We offers you
oligosaccharides which play important roles such as intercellular
recognition, adhesion, proliferation control and signaling.
Glycolipids
Products
Glycolipids Products
G0509 α-GalactosylCeramide
G0580 Gb4 Ceramide
G0592 Gb5 Ceramide (= SSEA-3)
G0589 Globo-H Ceramide
G0593 SialylGb5 Ceramide (= SSEA-4)
G0483 Ganglioside GM1
G0489 Ganglioside GM3
G0422 Ganglioside GM3 (phyto-type)
G0510 Ganglioside GM3(Neu5Gc) (phyto-type)
S0910 Sialyl Neolactotetraosylceramide (= Sialyl nLc4Cer)
Oligosaccharides
Products
Oligosaccharides Products
G0479 Globotriose
G0403 Gb3-β-ethylazide
G0402 Gb3-β-ethylamine
G0623 Gb4-PrN3
G0434 Globopentaose
G0599 Gb5-PrN3
G0596 Globo-H-PrN3
G0447 Globo-H-PrNH2
S0946 SSEA-1-PrNH2
S0849 Sialyl Lewis X-Lactose
N1147 Neu5Acα(2-6)Gal-β-propylamine
N0950 Neu5Acα(2-6)Galβ(1-4)GlcNAc-β-ethylamine
N1249 Neu5Acα(2-3)Gal-β-propylazide
N1146 Neu5Acα(2-3)Gal-β-propylamine
N1267 Neu5Acα(2-3)Galβ(1-4)GlcNAc-β-propylamine
N1258 Neu5Acα(2-3)Galβ(1-4)Glc-β-propylamine
N1259 Neu5Gcα(2-3)Galβ(1-4)Glc-β-propylamine
Anti-glycolipid Antibodies
• Antibodies are proteins which are one of the components of the
immune system. The specificity of antibodies is likened to the
interaction between a key and a keyhole. Anti-glycolipid antibodies
can specifically recognize glycolipids.
• TCI mainly produce antibodies against glycolipids; Ganglio-series,
Globo-series, Lacto-series, and Neolacto-series.
• These antibodies can be used for immunohistochemistry, cell-
staining, flow cytometry, ELISA, TLC-immunostaining and other
methods.
• Our antibodies are very useful tools for analyzing the expression of
carbohydrate chains and their functions.
Products
A2505 Anti-GM1 Monoclonal Antibody
A2576 Anti-GM2 Monoclonal Antibody
A2582 Anti-GM3 Monoclonal Antibody
A2507 Anti-GD1a Monoclonal Antibody
Anti-glycolipid Antibodies
Anti-glycolipid Antibodies Products
A2508 Anti-GD1b Monoclonal Antibody
A3338 Anti-GD2 Monoclonal Antibody
A2580 Anti-GD3 Monoclonal Antibody
A2702 Anti-GT1a Monoclonal Antibody
A2732 Anti-GT1b Monoclonal Antibody
A2662 Anti-GQ1b Monoclonal Antibody
A2701 Anti-GalNAc-GD1a Monoclonal Antibody (2A3D2)
A2506 Anti-Gb3 Monoclonal Antibody
A2822 Anti-Gb3 Monoclonal Antibody Biotin Conjugate
A2706 Anti-SGPG (HNK-1) Monoclonal Antibody
Lectins
• Lectins are highly specific carbohydrate-binding proteins of
nonimmune origin. Due to their ability to bind with cell-surface
glycoproteins and glycolipids, lectins could agglutinate cells; they also
could reversibly associate polysaccharides and glycoproteins in
solution.
• Lectin has long been well-known as tools for detection and analysis of
functional oligosaccharides in the glycoscience field. Recombinant
lectins show better stability compared with lectins extracted from
natural resources.
• TCI offers not only recombinant lectins but also chemically modified
lectins such as several types of biotinylated lectin and lectin-agarose
for detection and capturing of glycoconjugates.
LecBeads (Lectin-Agarose)
Products
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
Lectin-Biotin Conjugates
Products
R0230 rPSL1a-Biotin [for Siaα(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
Lectins
Products
R0225 Recombinant Polyporus squamosus lectin (= rPSL1a) expressed in E. coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in E. coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in E. coli
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in E. coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in E. coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae
Endoglycosidases
• Endoglycoceramidase (EGCase) is a glycolipid-specific hydrolase that
cleaves the glycosidic linkage between oligosaccharide and ceramide
of various glycosphingolipids (GSLs).
• Recombinant EGCase (rEGCase) is activated under a detergent-
dependent condition. In contrast, Activator II is capable of inducing
activity of EGCases without any detergent reagents.
• By the use of Activator II, GSLs on cell surfaces of living cells could be
hydrolyzed without cell disruption caused by detergent.
Endoglycosidases and Related Enzymes
Products
R0240 rEGCase I
R0241 rEGCase I assisted by Activator II
R0242 rEGCase II
R0243 rEGCase II assisted by Activator II
A1651 Endo-M (= endo-β-N-Acetylglucosaminidase)
G0365 Glycosynthase (= Endo-M-N175Q)
E1339 Endo-M-W251N
A1844 endo-α-N-Acetylgalactosaminidase (=Endo-α)
K0069 Keratanase II

More Related Content

Similar to Glycan-related Reagents for Extracellular Vesicles (EVs) Research

Triacylglycarois and structure of it,yes
Triacylglycarois and structure of it,yesTriacylglycarois and structure of it,yes
Triacylglycarois and structure of it,yes
bdfkjph567
 
Microbial taxonomy
Microbial taxonomyMicrobial taxonomy
Microbial taxonomy
KARTHIK REDDY C A
 
Glycosaminoglycams+Glycoconjugates (2) final.pptx
Glycosaminoglycams+Glycoconjugates (2) final.pptxGlycosaminoglycams+Glycoconjugates (2) final.pptx
Glycosaminoglycams+Glycoconjugates (2) final.pptx
MarryamFaiz
 
Chemistry of carbohydrates
Chemistry of carbohydratesChemistry of carbohydrates
Chemistry of carbohydrates
enamifat
 
4. glycosides Full notes for presentation and examination pharmacognosy
4. glycosides Full notes for presentation and examination pharmacognosy4. glycosides Full notes for presentation and examination pharmacognosy
4. glycosides Full notes for presentation and examination pharmacognosy
Dr Muhammad Bilal
 
bicatalysispresentation1-211210145704.pptx
bicatalysispresentation1-211210145704.pptxbicatalysispresentation1-211210145704.pptx
bicatalysispresentation1-211210145704.pptx
alizain9604
 
JBEI Research Highlights November 2016
JBEI Research Highlights November 2016JBEI Research Highlights November 2016
JBEI Research Highlights November 2016
Irina Silva
 
JBEI Research Highlights - February 2018
JBEI Research Highlights - February 2018JBEI Research Highlights - February 2018
JBEI Research Highlights - February 2018
Irina Silva
 
Heteropolysaccharides
HeteropolysaccharidesHeteropolysaccharides
Heteropolysaccharides
Shamim Akram
 
Bacterial Neuraminidase Simpson Lab
Bacterial Neuraminidase Simpson LabBacterial Neuraminidase Simpson Lab
Bacterial Neuraminidase Simpson Lab
Rosemary Cullander
 
Carrageenase enzyme
Carrageenase enzymeCarrageenase enzyme
Carrageenase enzyme
Madhav Kumar
 
Glycolysis Rection JNVU
Glycolysis Rection JNVUGlycolysis Rection JNVU
Glycolysis Rection JNVU
BHARAT NOGIA
 
Glycoproteins
Glycoproteins Glycoproteins
Glycoproteins
Dr. Kapil Dev Doddamani
 
Antioxidant effect of propolis extract on liver
Antioxidant effect of propolis extract on liverAntioxidant effect of propolis extract on liver
Antioxidant effect of propolis extract on liver
Bee Healthy Farms
 
Wine Microbiology for Fermentation Success and Wine Quality
Wine Microbiology for Fermentation Success and Wine QualityWine Microbiology for Fermentation Success and Wine Quality
Wine Microbiology for Fermentation Success and Wine Quality
Waite Research Institute
 
cell signaling Presentation varrious pathways of cell signaling .physiology.pptx
cell signaling Presentation varrious pathways of cell signaling .physiology.pptxcell signaling Presentation varrious pathways of cell signaling .physiology.pptx
cell signaling Presentation varrious pathways of cell signaling .physiology.pptx
drkhadarpasha
 
1_merged (1).pptx
1_merged (1).pptx1_merged (1).pptx
1_merged (1).pptx
EYOBDEBEBEAREGA
 
Carbohydrates
CarbohydratesCarbohydrates
JBEI Highlights - October 2014
JBEI Highlights - October 2014JBEI Highlights - October 2014
JBEI Highlights - October 2014
Irina Silva
 
Heme binding to GAPDH_Biochemistry 2012
Heme binding to GAPDH_Biochemistry 2012Heme binding to GAPDH_Biochemistry 2012
Heme binding to GAPDH_Biochemistry 2012
Luciana Hannibal, Ph.D.
 

Similar to Glycan-related Reagents for Extracellular Vesicles (EVs) Research (20)

Triacylglycarois and structure of it,yes
Triacylglycarois and structure of it,yesTriacylglycarois and structure of it,yes
Triacylglycarois and structure of it,yes
 
Microbial taxonomy
Microbial taxonomyMicrobial taxonomy
Microbial taxonomy
 
Glycosaminoglycams+Glycoconjugates (2) final.pptx
Glycosaminoglycams+Glycoconjugates (2) final.pptxGlycosaminoglycams+Glycoconjugates (2) final.pptx
Glycosaminoglycams+Glycoconjugates (2) final.pptx
 
Chemistry of carbohydrates
Chemistry of carbohydratesChemistry of carbohydrates
Chemistry of carbohydrates
 
4. glycosides Full notes for presentation and examination pharmacognosy
4. glycosides Full notes for presentation and examination pharmacognosy4. glycosides Full notes for presentation and examination pharmacognosy
4. glycosides Full notes for presentation and examination pharmacognosy
 
bicatalysispresentation1-211210145704.pptx
bicatalysispresentation1-211210145704.pptxbicatalysispresentation1-211210145704.pptx
bicatalysispresentation1-211210145704.pptx
 
JBEI Research Highlights November 2016
JBEI Research Highlights November 2016JBEI Research Highlights November 2016
JBEI Research Highlights November 2016
 
JBEI Research Highlights - February 2018
JBEI Research Highlights - February 2018JBEI Research Highlights - February 2018
JBEI Research Highlights - February 2018
 
Heteropolysaccharides
HeteropolysaccharidesHeteropolysaccharides
Heteropolysaccharides
 
Bacterial Neuraminidase Simpson Lab
Bacterial Neuraminidase Simpson LabBacterial Neuraminidase Simpson Lab
Bacterial Neuraminidase Simpson Lab
 
Carrageenase enzyme
Carrageenase enzymeCarrageenase enzyme
Carrageenase enzyme
 
Glycolysis Rection JNVU
Glycolysis Rection JNVUGlycolysis Rection JNVU
Glycolysis Rection JNVU
 
Glycoproteins
Glycoproteins Glycoproteins
Glycoproteins
 
Antioxidant effect of propolis extract on liver
Antioxidant effect of propolis extract on liverAntioxidant effect of propolis extract on liver
Antioxidant effect of propolis extract on liver
 
Wine Microbiology for Fermentation Success and Wine Quality
Wine Microbiology for Fermentation Success and Wine QualityWine Microbiology for Fermentation Success and Wine Quality
Wine Microbiology for Fermentation Success and Wine Quality
 
cell signaling Presentation varrious pathways of cell signaling .physiology.pptx
cell signaling Presentation varrious pathways of cell signaling .physiology.pptxcell signaling Presentation varrious pathways of cell signaling .physiology.pptx
cell signaling Presentation varrious pathways of cell signaling .physiology.pptx
 
1_merged (1).pptx
1_merged (1).pptx1_merged (1).pptx
1_merged (1).pptx
 
Carbohydrates
CarbohydratesCarbohydrates
Carbohydrates
 
JBEI Highlights - October 2014
JBEI Highlights - October 2014JBEI Highlights - October 2014
JBEI Highlights - October 2014
 
Heme binding to GAPDH_Biochemistry 2012
Heme binding to GAPDH_Biochemistry 2012Heme binding to GAPDH_Biochemistry 2012
Heme binding to GAPDH_Biochemistry 2012
 

Recently uploaded

Potential of Marine renewable and Non renewable energy.pptx
Potential of Marine renewable and Non renewable energy.pptxPotential of Marine renewable and Non renewable energy.pptx
Potential of Marine renewable and Non renewable energy.pptx
J. Bovas Joel BFSc
 
SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...
SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...
SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...
Sérgio Sacani
 
The CGIAR needs a revolution John McIntire a, Achim Dobermann b
The CGIAR needs a revolution John McIntire a, Achim Dobermann bThe CGIAR needs a revolution John McIntire a, Achim Dobermann b
The CGIAR needs a revolution John McIntire a, Achim Dobermann b
Abdellah HAMMA
 
All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...
All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...
All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...
Sérgio Sacani
 
Composting blue materials - Joshua Cabell
Composting blue materials - Joshua CabellComposting blue materials - Joshua Cabell
Composting blue materials - Joshua Cabell
Faculty of Applied Chemistry and Materials Science
 
Analytical methods for blue residues characterization - Oana Crina Bujor
Analytical methods for blue residues characterization - Oana Crina BujorAnalytical methods for blue residues characterization - Oana Crina Bujor
Analytical methods for blue residues characterization - Oana Crina Bujor
Faculty of Applied Chemistry and Materials Science
 
A hot-Jupiter progenitor on a super-eccentric retrograde orbit
A hot-Jupiter progenitor on a super-eccentric retrograde orbitA hot-Jupiter progenitor on a super-eccentric retrograde orbit
A hot-Jupiter progenitor on a super-eccentric retrograde orbit
Sérgio Sacani
 
Animal biotechnology Cattle breeds....pptx
Animal biotechnology Cattle breeds....pptxAnimal biotechnology Cattle breeds....pptx
Animal biotechnology Cattle breeds....pptx
Ritesh Chavan
 
atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...
atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...
atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...
ManjulaVani3
 
Simulations of pulsed overpressure jets: formation of bellows and ripples in ...
Simulations of pulsed overpressure jets: formation of bellows and ripples in ...Simulations of pulsed overpressure jets: formation of bellows and ripples in ...
Simulations of pulsed overpressure jets: formation of bellows and ripples in ...
Sérgio Sacani
 
Synopsis: Analysis of a Metallic Specimen
Synopsis: Analysis of a Metallic SpecimenSynopsis: Analysis of a Metallic Specimen
Synopsis: Analysis of a Metallic Specimen
Sérgio Sacani
 
Bacteria l classification(sperical).pptx
Bacteria l classification(sperical).pptxBacteria l classification(sperical).pptx
Bacteria l classification(sperical).pptx
shubhamve111yadav
 
MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)
MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)
MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)
Steffi Friedrichs
 
Rice Genome Project a complete saga .(1).pptx
Rice Genome  Project a complete saga .(1).pptxRice Genome  Project a complete saga .(1).pptx
Rice Genome Project a complete saga .(1).pptx
SoumyaDixit11
 
From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...
From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...
From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...
Sérgio Sacani
 
A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715
A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715
A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715
Sérgio Sacani
 
Ancient Theory, Abiogenesis , Biogenesis
Ancient Theory, Abiogenesis , BiogenesisAncient Theory, Abiogenesis , Biogenesis
Ancient Theory, Abiogenesis , Biogenesis
SoniaBajaj10
 
Rapid pulse drying of marine biomasses - Sigurd Sannan
Rapid pulse drying of marine biomasses - Sigurd SannanRapid pulse drying of marine biomasses - Sigurd Sannan
Rapid pulse drying of marine biomasses - Sigurd Sannan
Faculty of Applied Chemistry and Materials Science
 
Post RN - Biochemistry (Unit 7) Metabolism
Post RN - Biochemistry (Unit 7) MetabolismPost RN - Biochemistry (Unit 7) Metabolism
Post RN - Biochemistry (Unit 7) Metabolism
Areesha Ahmad
 
Fish in the Loop: Exploring RAS - Julie Hansen Bergstedt
Fish in the Loop: Exploring RAS - Julie Hansen BergstedtFish in the Loop: Exploring RAS - Julie Hansen Bergstedt
Fish in the Loop: Exploring RAS - Julie Hansen Bergstedt
Faculty of Applied Chemistry and Materials Science
 

Recently uploaded (20)

Potential of Marine renewable and Non renewable energy.pptx
Potential of Marine renewable and Non renewable energy.pptxPotential of Marine renewable and Non renewable energy.pptx
Potential of Marine renewable and Non renewable energy.pptx
 
SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...
SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...
SOFIA/HAWC+ FAR-INFRARED POLARIMETRIC LARGE-AREA CMZ EXPLORATION (FIREPLACE) ...
 
The CGIAR needs a revolution John McIntire a, Achim Dobermann b
The CGIAR needs a revolution John McIntire a, Achim Dobermann bThe CGIAR needs a revolution John McIntire a, Achim Dobermann b
The CGIAR needs a revolution John McIntire a, Achim Dobermann b
 
All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...
All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...
All-domain Anomaly Resolution Office Supplement to Oak Ridge National Laborat...
 
Composting blue materials - Joshua Cabell
Composting blue materials - Joshua CabellComposting blue materials - Joshua Cabell
Composting blue materials - Joshua Cabell
 
Analytical methods for blue residues characterization - Oana Crina Bujor
Analytical methods for blue residues characterization - Oana Crina BujorAnalytical methods for blue residues characterization - Oana Crina Bujor
Analytical methods for blue residues characterization - Oana Crina Bujor
 
A hot-Jupiter progenitor on a super-eccentric retrograde orbit
A hot-Jupiter progenitor on a super-eccentric retrograde orbitA hot-Jupiter progenitor on a super-eccentric retrograde orbit
A hot-Jupiter progenitor on a super-eccentric retrograde orbit
 
Animal biotechnology Cattle breeds....pptx
Animal biotechnology Cattle breeds....pptxAnimal biotechnology Cattle breeds....pptx
Animal biotechnology Cattle breeds....pptx
 
atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...
atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...
atom, elements, molecule and compounds #CBSE, #IX class, #chapter-3, #ATOMS&M...
 
Simulations of pulsed overpressure jets: formation of bellows and ripples in ...
Simulations of pulsed overpressure jets: formation of bellows and ripples in ...Simulations of pulsed overpressure jets: formation of bellows and ripples in ...
Simulations of pulsed overpressure jets: formation of bellows and ripples in ...
 
Synopsis: Analysis of a Metallic Specimen
Synopsis: Analysis of a Metallic SpecimenSynopsis: Analysis of a Metallic Specimen
Synopsis: Analysis of a Metallic Specimen
 
Bacteria l classification(sperical).pptx
Bacteria l classification(sperical).pptxBacteria l classification(sperical).pptx
Bacteria l classification(sperical).pptx
 
MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)
MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)
MACRAMÉ ChIPs @Behoerdenklausur 2024 (Berlin)
 
Rice Genome Project a complete saga .(1).pptx
Rice Genome  Project a complete saga .(1).pptxRice Genome  Project a complete saga .(1).pptx
Rice Genome Project a complete saga .(1).pptx
 
From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...
From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...
From Seeds to Supermassive Black Holes: Capture, Growth, Migration, and Pairi...
 
A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715
A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715
A NICER VIEW OF THE NEAREST AND BRIGHTEST MILLISECOND PULSAR: PSR J0437−4715
 
Ancient Theory, Abiogenesis , Biogenesis
Ancient Theory, Abiogenesis , BiogenesisAncient Theory, Abiogenesis , Biogenesis
Ancient Theory, Abiogenesis , Biogenesis
 
Rapid pulse drying of marine biomasses - Sigurd Sannan
Rapid pulse drying of marine biomasses - Sigurd SannanRapid pulse drying of marine biomasses - Sigurd Sannan
Rapid pulse drying of marine biomasses - Sigurd Sannan
 
Post RN - Biochemistry (Unit 7) Metabolism
Post RN - Biochemistry (Unit 7) MetabolismPost RN - Biochemistry (Unit 7) Metabolism
Post RN - Biochemistry (Unit 7) Metabolism
 
Fish in the Loop: Exploring RAS - Julie Hansen Bergstedt
Fish in the Loop: Exploring RAS - Julie Hansen BergstedtFish in the Loop: Exploring RAS - Julie Hansen Bergstedt
Fish in the Loop: Exploring RAS - Julie Hansen Bergstedt
 

Glycan-related Reagents for Extracellular Vesicles (EVs) Research

  • 2. Extracellular Vesicles • Extracellular Vesicles (EVs) are small vesicles with lipid bilayer that are secreted from various cells under physiological and pathological conditions and exist in the body fluids and cell culture supernatant. • EVs contain nucleic acids and proteins, and act as mediators of intercellular communication by entering the target cells. • EVs include subtypes such as exosomes and microvesicles. • They are involved in many physiological responses and diseases, including cell differentiation, immune responses, infection, cancer, cardiovascular diseases, and neurodegeneration.
  • 3. Extracellular Vesicles • EVs derived from mesenchymal stem cells are known to have therapeutic potential, with several clinical trials ongoing. • EV applications to drug delivery platforms have also been developed.
  • 4. Glycans • Glycans exist not only on the surface of cells, but also on the surface of sEVs (small Extracellular Vesicles: EVs <200 nm in diameter) as glycoproteins or glycolipids. • Importance of glycans on EVs has been shown. For example, glycocalyx on the cancer cell-derived EVs has been reported to involve in organotropic determination in cancer metastases. • Glycans on the EV have been reported to play important role in the tuning of EV uptake. TCI has many products related to glycans expressed on the EVs.
  • 5. Glycolipid-related Glycans • Glycan patterns are well known to change during various biological events, which include cancer, inflammatory diseases, and immune diseases, and the same applies to sEV surface glycans. TCI can synthesize various glycans on the cell surface. We offers you oligosaccharides which play important roles such as intercellular recognition, adhesion, proliferation control and signaling.
  • 7. Glycolipids Products G0509 α-GalactosylCeramide G0580 Gb4 Ceramide G0592 Gb5 Ceramide (= SSEA-3) G0589 Globo-H Ceramide G0593 SialylGb5 Ceramide (= SSEA-4) G0483 Ganglioside GM1 G0489 Ganglioside GM3 G0422 Ganglioside GM3 (phyto-type) G0510 Ganglioside GM3(Neu5Gc) (phyto-type) S0910 Sialyl Neolactotetraosylceramide (= Sialyl nLc4Cer)
  • 9. Oligosaccharides Products G0479 Globotriose G0403 Gb3-β-ethylazide G0402 Gb3-β-ethylamine G0623 Gb4-PrN3 G0434 Globopentaose G0599 Gb5-PrN3 G0596 Globo-H-PrN3 G0447 Globo-H-PrNH2 S0946 SSEA-1-PrNH2 S0849 Sialyl Lewis X-Lactose N1147 Neu5Acα(2-6)Gal-β-propylamine N0950 Neu5Acα(2-6)Galβ(1-4)GlcNAc-β-ethylamine N1249 Neu5Acα(2-3)Gal-β-propylazide N1146 Neu5Acα(2-3)Gal-β-propylamine N1267 Neu5Acα(2-3)Galβ(1-4)GlcNAc-β-propylamine N1258 Neu5Acα(2-3)Galβ(1-4)Glc-β-propylamine N1259 Neu5Gcα(2-3)Galβ(1-4)Glc-β-propylamine
  • 10. Anti-glycolipid Antibodies • Antibodies are proteins which are one of the components of the immune system. The specificity of antibodies is likened to the interaction between a key and a keyhole. Anti-glycolipid antibodies can specifically recognize glycolipids. • TCI mainly produce antibodies against glycolipids; Ganglio-series, Globo-series, Lacto-series, and Neolacto-series. • These antibodies can be used for immunohistochemistry, cell- staining, flow cytometry, ELISA, TLC-immunostaining and other methods. • Our antibodies are very useful tools for analyzing the expression of carbohydrate chains and their functions.
  • 11. Products A2505 Anti-GM1 Monoclonal Antibody A2576 Anti-GM2 Monoclonal Antibody A2582 Anti-GM3 Monoclonal Antibody A2507 Anti-GD1a Monoclonal Antibody Anti-glycolipid Antibodies
  • 12. Anti-glycolipid Antibodies Products A2508 Anti-GD1b Monoclonal Antibody A3338 Anti-GD2 Monoclonal Antibody A2580 Anti-GD3 Monoclonal Antibody A2702 Anti-GT1a Monoclonal Antibody A2732 Anti-GT1b Monoclonal Antibody A2662 Anti-GQ1b Monoclonal Antibody A2701 Anti-GalNAc-GD1a Monoclonal Antibody (2A3D2) A2506 Anti-Gb3 Monoclonal Antibody A2822 Anti-Gb3 Monoclonal Antibody Biotin Conjugate A2706 Anti-SGPG (HNK-1) Monoclonal Antibody
  • 13. Lectins • Lectins are highly specific carbohydrate-binding proteins of nonimmune origin. Due to their ability to bind with cell-surface glycoproteins and glycolipids, lectins could agglutinate cells; they also could reversibly associate polysaccharides and glycoproteins in solution. • Lectin has long been well-known as tools for detection and analysis of functional oligosaccharides in the glycoscience field. Recombinant lectins show better stability compared with lectins extracted from natural resources. • TCI offers not only recombinant lectins but also chemically modified lectins such as several types of biotinylated lectin and lectin-agarose for detection and capturing of glycoconjugates.
  • 14. LecBeads (Lectin-Agarose) Products R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal] R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc] R0237 rMOA-LecBeads [for Galα(1-3)Gal] R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] R0239 rGRFT-LecBeads [for Manα(1-2)Man] A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
  • 15. Lectin-Biotin Conjugates Products R0230 rPSL1a-Biotin [for Siaα(2-6)Gal] R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc] R0232 rMOA-Biotin [for Galα(1-3)Gal] R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] R0234 rGRFT-Biotin [for Manα(1-2)Man] A2659 AOL-Biotin Conjugate
  • 16. Lectins Products R0225 Recombinant Polyporus squamosus lectin (= rPSL1a) expressed in E. coli R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in E. coli R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in E. coli R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in E. coli R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in E. coli L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae
  • 17. Endoglycosidases • Endoglycoceramidase (EGCase) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs). • Recombinant EGCase (rEGCase) is activated under a detergent- dependent condition. In contrast, Activator II is capable of inducing activity of EGCases without any detergent reagents. • By the use of Activator II, GSLs on cell surfaces of living cells could be hydrolyzed without cell disruption caused by detergent.
  • 18. Endoglycosidases and Related Enzymes Products R0240 rEGCase I R0241 rEGCase I assisted by Activator II R0242 rEGCase II R0243 rEGCase II assisted by Activator II A1651 Endo-M (= endo-β-N-Acetylglucosaminidase) G0365 Glycosynthase (= Endo-M-N175Q) E1339 Endo-M-W251N A1844 endo-α-N-Acetylgalactosaminidase (=Endo-α) K0069 Keratanase II