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KELOMPOK 6
Aditya Rahmat
Dinda Rahma Juita
Syarafina Sabila
Bahan Organik
VITAMIN A
VITAMIN
Larut Dalam Air Larut Dalam Lemak
VITAMIN A
Vitamin A (Retinol)
 Peran
1. Pembentukan indra penglihatan
2. Penyusun komponen pigmen
mata
 Sifat
1. Mudah rusak oleh sinar UV & oksidasi
2. Stabil pada temperatur tinggi
 Struktur dan Senyawa Vitamin A
- Senyawa hidrokarbon tidak jenuh C20 dan
C40,
- Bentuk Isomer yang penting :
a. Vitamin A1 = Retinol1 (C20H29OH)
b. Vitamin A2 = Retinol 2 = 3-Dehidro
retinol (C20H27OH)
Struktur Vitamin A
 Golongan Vitamin A
1. Preform vitamin A → 4 bentuk aktif:
a. Alkohol → Retinol
b. Aldehid → Retinal / Retinaldehid
c. Asam → Asam Retinoat
d. Ester → Retinil asetat / Palmitat
2. PROVITAMIN A (Karotenoid)
Karotenoid yang mempunyai aktivitas
vitamin A
Ada > 600 karotenoid, aktivitas pro vitamin A
hanya +10% ( β-karoten, α-karoten, γ-karoten)
Bahan makanan
(Provitamin A)
Umumnya
terdapat dalam
bentuk Retinil
Palmitat
Di konsumsi
(Previtamin A)
S U M B E R
V I T A M I N A
N A B A
T I
HEWANI
HEWANI N A B A
T I
Metode Analisis Vitamin A
Metode
.
Spektrofotometri
HPLC
Kromatografi
• Pengukuran serapan sinar
monokromatis
• Prinsip = Hukum Lambert-
Beer
H P L C
• Prinsip = Kromatografi
• Memisahkan berdasarkan perbedaan
struktur/komposisi
• Sampel melewati fase diam
PRESENTATION
- Memisahkan campuran yang tidak volatile
- berdasarkan adsorpsi dan partisi
Kromatografi Lapis TipisSilica Gel
Aluminium Oksida
Selulosa
ALATBAHAN
HEKSANA ASETON SAMPEL
Cara Kerja
Biarkan hingga
terpisah
Spotting hingga
6 -10 kali
+ 2 tetes
aseton
+ 1 tetes
heksana
+ 7 ml
heksana
ANY
QUESTION??
Thank You

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Analis Bahan Organik - Vitamin A

  • 1. KELOMPOK 6 Aditya Rahmat Dinda Rahma Juita Syarafina Sabila Bahan Organik VITAMIN A
  • 2. VITAMIN Larut Dalam Air Larut Dalam Lemak VITAMIN A
  • 3. Vitamin A (Retinol)  Peran 1. Pembentukan indra penglihatan 2. Penyusun komponen pigmen mata  Sifat 1. Mudah rusak oleh sinar UV & oksidasi 2. Stabil pada temperatur tinggi  Struktur dan Senyawa Vitamin A - Senyawa hidrokarbon tidak jenuh C20 dan C40, - Bentuk Isomer yang penting : a. Vitamin A1 = Retinol1 (C20H29OH) b. Vitamin A2 = Retinol 2 = 3-Dehidro retinol (C20H27OH)
  • 4. Struktur Vitamin A  Golongan Vitamin A 1. Preform vitamin A → 4 bentuk aktif: a. Alkohol → Retinol b. Aldehid → Retinal / Retinaldehid c. Asam → Asam Retinoat d. Ester → Retinil asetat / Palmitat
  • 5. 2. PROVITAMIN A (Karotenoid) Karotenoid yang mempunyai aktivitas vitamin A Ada > 600 karotenoid, aktivitas pro vitamin A hanya +10% ( β-karoten, α-karoten, γ-karoten)
  • 6. Bahan makanan (Provitamin A) Umumnya terdapat dalam bentuk Retinil Palmitat Di konsumsi (Previtamin A)
  • 7. S U M B E R V I T A M I N A N A B A T I HEWANI HEWANI N A B A T I
  • 8. Metode Analisis Vitamin A Metode . Spektrofotometri HPLC Kromatografi • Pengukuran serapan sinar monokromatis • Prinsip = Hukum Lambert- Beer
  • 9. H P L C • Prinsip = Kromatografi • Memisahkan berdasarkan perbedaan struktur/komposisi • Sampel melewati fase diam
  • 10. PRESENTATION - Memisahkan campuran yang tidak volatile - berdasarkan adsorpsi dan partisi Kromatografi Lapis TipisSilica Gel Aluminium Oksida Selulosa
  • 12. Cara Kerja Biarkan hingga terpisah Spotting hingga 6 -10 kali + 2 tetes aseton + 1 tetes heksana + 7 ml heksana