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SUBMIT BY
SRIDHARSHINI S
22MBO025
2nd-M.Sc.,BOTANY
HERBICIDE RESISTANCE -
PHOSPHINOTHRICIN
WEEDS
 Weeds are unwanted,
persistent, harmful plants that
impede the growth of other crop
plants.
 Undesirable weeds are plants
that grow alongside the main
plants.
 Herbicides are chemicals used
to manipulate or control
undesirable vegetation.
 Herbicide application occurs
most frequently in row-crop
farming, where they are applied
before or during planting to
maximize crop productivity.
HERBICIDE
Cross resistance
Weed has gained resistance
to more than 1 herbicide
with same mode of action.
Multiple resistance
Weed has gained tolerance
to more than 1 herbicide
with the different mode of
action.
HERBICIDE RESISTANCE
 Gulfosinate ammonium(GA) is a non selective pro-herbicide
that is converted by plants into phytotoxin.
Phosphinothricin(PPT).
 The phosphinothricin inhibits the production of glutamine
synthase(GS).
 The gulfosinate was discovered as an antibiotic produced by
fungus, Streptomyces hygroscopicus.
 Absence of glutamine synthase leads to the accumulation of
ammonium in plants, later on it leads to lethal condition.
ACTION of TRANSGENIC
HERBICIDE PLANTS
BILAPHOS or BASTA
Leads to death of plants
phOSPHINOTHRICIn
INHIBITION OF GLUTAMINE
SYNTHAES
Accumilation of
ammonium
 Bar gene (bialaphos resistance), which encodes a
phosphinothricin acetyl transferase(PAT) that converts the
herbicidal molecule to a non-toxic acetyl form.
 The acetyl phosphinothricin inactivates the glutamine
synthase.
 The production of glutamine synthase restrict the
accumulation of ammonium and its proceeds the plant to
perform the normal activity.
DETOXIFICATION OF
PHOSPHINOTHRICIN
Resistant gene
PAT(Phosphinothricin acetyl transferase)
Leads to proper function of plants
BAR gene
PRODUCTION of PAT gene
Inactivation of
phosphinothricin
1. Cron
2. Rice
3. Wheat
4. Sugarbeet
5. Oilseed rape and alfalfa
6. Cotton
7. Lettuce
8. Sugarcane
9. Cassava
10. Dry beans
PHOSPHINOTHRICIN HERBICIDE
RESISTANCE CROPS
1. Border spectrum of weeds controlled.
2. Reduction of crop injury, herbicide carry-over.
3. Use of transgenic herbicides that are more
environmental friendly.
4. Crop mamnagement flexibility and simplicity.
ADVANTAGES
1. Single selection pressure and weed
resistance.
2. There is a shifts in weed species.
3. Gene escapes, gene flow and contamination
of organic crops.
4. Ecotoxicity(side effects on soil
microorganisms and agricultural flora and
fauna).
DISADVANTAGES
THANK YOU

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genetic engineering- phosphinothricin inhibition.pptx

  • 2. WEEDS  Weeds are unwanted, persistent, harmful plants that impede the growth of other crop plants.  Undesirable weeds are plants that grow alongside the main plants.
  • 3.  Herbicides are chemicals used to manipulate or control undesirable vegetation.  Herbicide application occurs most frequently in row-crop farming, where they are applied before or during planting to maximize crop productivity. HERBICIDE
  • 4. Cross resistance Weed has gained resistance to more than 1 herbicide with same mode of action. Multiple resistance Weed has gained tolerance to more than 1 herbicide with the different mode of action. HERBICIDE RESISTANCE
  • 5.  Gulfosinate ammonium(GA) is a non selective pro-herbicide that is converted by plants into phytotoxin. Phosphinothricin(PPT).  The phosphinothricin inhibits the production of glutamine synthase(GS).  The gulfosinate was discovered as an antibiotic produced by fungus, Streptomyces hygroscopicus.  Absence of glutamine synthase leads to the accumulation of ammonium in plants, later on it leads to lethal condition. ACTION of TRANSGENIC HERBICIDE PLANTS
  • 6. BILAPHOS or BASTA Leads to death of plants phOSPHINOTHRICIn INHIBITION OF GLUTAMINE SYNTHAES Accumilation of ammonium
  • 7.  Bar gene (bialaphos resistance), which encodes a phosphinothricin acetyl transferase(PAT) that converts the herbicidal molecule to a non-toxic acetyl form.  The acetyl phosphinothricin inactivates the glutamine synthase.  The production of glutamine synthase restrict the accumulation of ammonium and its proceeds the plant to perform the normal activity. DETOXIFICATION OF PHOSPHINOTHRICIN
  • 8. Resistant gene PAT(Phosphinothricin acetyl transferase) Leads to proper function of plants BAR gene PRODUCTION of PAT gene Inactivation of phosphinothricin
  • 9. 1. Cron 2. Rice 3. Wheat 4. Sugarbeet 5. Oilseed rape and alfalfa 6. Cotton 7. Lettuce 8. Sugarcane 9. Cassava 10. Dry beans PHOSPHINOTHRICIN HERBICIDE RESISTANCE CROPS
  • 10. 1. Border spectrum of weeds controlled. 2. Reduction of crop injury, herbicide carry-over. 3. Use of transgenic herbicides that are more environmental friendly. 4. Crop mamnagement flexibility and simplicity. ADVANTAGES
  • 11. 1. Single selection pressure and weed resistance. 2. There is a shifts in weed species. 3. Gene escapes, gene flow and contamination of organic crops. 4. Ecotoxicity(side effects on soil microorganisms and agricultural flora and fauna). DISADVANTAGES