SlideShare a Scribd company logo
1 of 11
PAPER ELECTROPHORESIS
Presented by:
A. Sireesha Bai
M pharmacy first year
Department of pharmaceutical analysis
Santhiram college of pharmacy
PRINCPLE:
The charge carried by a molecule depends on the ph of the
medium. Electrophoresis at low voltage is not usually to
separate low molecular weight compounds because of
diffusion, but it is easier to illustrate the relationship
between charge and ph with amino acids than with
protiens or other macromolecules.
APPLICATIONS:
Serum analysis for diagnostic purpose is routinely
carried about by paper electrophoresis.
Muscle proteins, egg white proteins, milk proteins,
and snake insect venom analysis done by this technique.
Paper Electrophoresis: Separating Molecules by Charge

More Related Content

What's hot

Mass spectrometry basic principle & Instrumentation
Mass spectrometry basic principle & InstrumentationMass spectrometry basic principle & Instrumentation
Mass spectrometry basic principle & Instrumentationmanojjeya
 
immunological assays - ELISA and RIA
immunological assays - ELISA and RIAimmunological assays - ELISA and RIA
immunological assays - ELISA and RIApaideeksha
 
Moving Boundary Electrophoresis by Anamika Dey
Moving Boundary Electrophoresis by Anamika DeyMoving Boundary Electrophoresis by Anamika Dey
Moving Boundary Electrophoresis by Anamika DeyKushal Saha
 
Mass spectroscopy
Mass spectroscopyMass spectroscopy
Mass spectroscopyVINOTH R
 
Quadrupole and Time of Flight Mass analysers.
 Quadrupole and Time of Flight Mass analysers. Quadrupole and Time of Flight Mass analysers.
Quadrupole and Time of Flight Mass analysers.Gagangowda58
 
X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...
X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...
X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...Shriyansh Srivastav
 
Applications of mass spectrometry
Applications of mass spectrometryApplications of mass spectrometry
Applications of mass spectrometryZainab&Sons
 
Nmr spectroscopy
Nmr spectroscopyNmr spectroscopy
Nmr spectroscopyAsma Ashraf
 
Mass spectrometry and ionization techniques
Mass spectrometry and ionization techniquesMass spectrometry and ionization techniques
Mass spectrometry and ionization techniquesSurbhi Narang
 
Capillary electrophoresis.pptx
Capillary electrophoresis.pptxCapillary electrophoresis.pptx
Capillary electrophoresis.pptxElizabeth Philip
 
Nuclear Magnetic Double Resonance (Decoupling).pptx
Nuclear Magnetic Double Resonance (Decoupling).pptxNuclear Magnetic Double Resonance (Decoupling).pptx
Nuclear Magnetic Double Resonance (Decoupling).pptxRushikeshTidake
 
SlideShare Presentation on Mass spectrophotometer
SlideShare Presentation on Mass spectrophotometerSlideShare Presentation on Mass spectrophotometer
SlideShare Presentation on Mass spectrophotometerNaveen K L
 

What's hot (20)

Capillary Electrophoresis
Capillary ElectrophoresisCapillary Electrophoresis
Capillary Electrophoresis
 
Mass spectrometry basic principle & Instrumentation
Mass spectrometry basic principle & InstrumentationMass spectrometry basic principle & Instrumentation
Mass spectrometry basic principle & Instrumentation
 
immunological assays - ELISA and RIA
immunological assays - ELISA and RIAimmunological assays - ELISA and RIA
immunological assays - ELISA and RIA
 
Moving Boundary Electrophoresis by Anamika Dey
Moving Boundary Electrophoresis by Anamika DeyMoving Boundary Electrophoresis by Anamika Dey
Moving Boundary Electrophoresis by Anamika Dey
 
Mass spectroscopy
Mass spectroscopyMass spectroscopy
Mass spectroscopy
 
Immunological assay
Immunological assayImmunological assay
Immunological assay
 
Quadrupole and Time of Flight Mass analysers.
 Quadrupole and Time of Flight Mass analysers. Quadrupole and Time of Flight Mass analysers.
Quadrupole and Time of Flight Mass analysers.
 
X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...
X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...
X- ray crystallography, Shriyansh Srivastava, M.Pharm (Department of Pharmaco...
 
Applications of mass spectrometry
Applications of mass spectrometryApplications of mass spectrometry
Applications of mass spectrometry
 
NMR Spectroscopy
NMR SpectroscopyNMR Spectroscopy
NMR Spectroscopy
 
Spin spin coupling
Spin spin couplingSpin spin coupling
Spin spin coupling
 
Nmr spectroscopy
Nmr spectroscopyNmr spectroscopy
Nmr spectroscopy
 
Mass spectrometry and ionization techniques
Mass spectrometry and ionization techniquesMass spectrometry and ionization techniques
Mass spectrometry and ionization techniques
 
Capillary electrophoresis.pptx
Capillary electrophoresis.pptxCapillary electrophoresis.pptx
Capillary electrophoresis.pptx
 
MASS SPECTROMETRY=.pdf
MASS SPECTROMETRY=.pdfMASS SPECTROMETRY=.pdf
MASS SPECTROMETRY=.pdf
 
Radio Immuno Assay
Radio Immuno AssayRadio Immuno Assay
Radio Immuno Assay
 
Nuclear Magnetic Double Resonance (Decoupling).pptx
Nuclear Magnetic Double Resonance (Decoupling).pptxNuclear Magnetic Double Resonance (Decoupling).pptx
Nuclear Magnetic Double Resonance (Decoupling).pptx
 
Mass spectrometry
Mass spectrometryMass spectrometry
Mass spectrometry
 
SlideShare Presentation on Mass spectrophotometer
SlideShare Presentation on Mass spectrophotometerSlideShare Presentation on Mass spectrophotometer
SlideShare Presentation on Mass spectrophotometer
 
Bioluminescence Assay
Bioluminescence AssayBioluminescence Assay
Bioluminescence Assay
 

Recently uploaded

Concept of Vouching. B.Com(Hons) /B.Compdf
Concept of Vouching. B.Com(Hons) /B.CompdfConcept of Vouching. B.Com(Hons) /B.Compdf
Concept of Vouching. B.Com(Hons) /B.CompdfUmakantAnnand
 
call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️
call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️
call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️9953056974 Low Rate Call Girls In Saket, Delhi NCR
 
_Math 4-Q4 Week 5.pptx Steps in Collecting Data
_Math 4-Q4 Week 5.pptx Steps in Collecting Data_Math 4-Q4 Week 5.pptx Steps in Collecting Data
_Math 4-Q4 Week 5.pptx Steps in Collecting DataJhengPantaleon
 
Alper Gobel In Media Res Media Component
Alper Gobel In Media Res Media ComponentAlper Gobel In Media Res Media Component
Alper Gobel In Media Res Media ComponentInMediaRes1
 
Sanyam Choudhary Chemistry practical.pdf
Sanyam Choudhary Chemistry practical.pdfSanyam Choudhary Chemistry practical.pdf
Sanyam Choudhary Chemistry practical.pdfsanyamsingh5019
 
Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions  for the students and aspirants of Chemistry12th.pptxOrganic Name Reactions  for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions for the students and aspirants of Chemistry12th.pptxVS Mahajan Coaching Centre
 
KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...
KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...
KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...M56BOOKSTORE PRODUCT/SERVICE
 
“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...
“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...
“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...Marc Dusseiller Dusjagr
 
Incoming and Outgoing Shipments in 1 STEP Using Odoo 17
Incoming and Outgoing Shipments in 1 STEP Using Odoo 17Incoming and Outgoing Shipments in 1 STEP Using Odoo 17
Incoming and Outgoing Shipments in 1 STEP Using Odoo 17Celine George
 
A Critique of the Proposed National Education Policy Reform
A Critique of the Proposed National Education Policy ReformA Critique of the Proposed National Education Policy Reform
A Critique of the Proposed National Education Policy ReformChameera Dedduwage
 
Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...
Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...
Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...Krashi Coaching
 
Introduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptxIntroduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptxpboyjonauth
 
URLs and Routing in the Odoo 17 Website App
URLs and Routing in the Odoo 17 Website AppURLs and Routing in the Odoo 17 Website App
URLs and Routing in the Odoo 17 Website AppCeline George
 
Introduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher EducationIntroduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher Educationpboyjonauth
 
BASLIQ CURRENT LOOKBOOK LOOKBOOK(1) (1).pdf
BASLIQ CURRENT LOOKBOOK  LOOKBOOK(1) (1).pdfBASLIQ CURRENT LOOKBOOK  LOOKBOOK(1) (1).pdf
BASLIQ CURRENT LOOKBOOK LOOKBOOK(1) (1).pdfSoniaTolstoy
 
How to Make a Pirate ship Primary Education.pptx
How to Make a Pirate ship Primary Education.pptxHow to Make a Pirate ship Primary Education.pptx
How to Make a Pirate ship Primary Education.pptxmanuelaromero2013
 
SOCIAL AND HISTORICAL CONTEXT - LFTVD.pptx
SOCIAL AND HISTORICAL CONTEXT - LFTVD.pptxSOCIAL AND HISTORICAL CONTEXT - LFTVD.pptx
SOCIAL AND HISTORICAL CONTEXT - LFTVD.pptxiammrhaywood
 
The basics of sentences session 2pptx copy.pptx
The basics of sentences session 2pptx copy.pptxThe basics of sentences session 2pptx copy.pptx
The basics of sentences session 2pptx copy.pptxheathfieldcps1
 

Recently uploaded (20)

Concept of Vouching. B.Com(Hons) /B.Compdf
Concept of Vouching. B.Com(Hons) /B.CompdfConcept of Vouching. B.Com(Hons) /B.Compdf
Concept of Vouching. B.Com(Hons) /B.Compdf
 
call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️
call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️
call girls in Kamla Market (DELHI) 🔝 >༒9953330565🔝 genuine Escort Service 🔝✔️✔️
 
_Math 4-Q4 Week 5.pptx Steps in Collecting Data
_Math 4-Q4 Week 5.pptx Steps in Collecting Data_Math 4-Q4 Week 5.pptx Steps in Collecting Data
_Math 4-Q4 Week 5.pptx Steps in Collecting Data
 
Alper Gobel In Media Res Media Component
Alper Gobel In Media Res Media ComponentAlper Gobel In Media Res Media Component
Alper Gobel In Media Res Media Component
 
Sanyam Choudhary Chemistry practical.pdf
Sanyam Choudhary Chemistry practical.pdfSanyam Choudhary Chemistry practical.pdf
Sanyam Choudhary Chemistry practical.pdf
 
Código Creativo y Arte de Software | Unidad 1
Código Creativo y Arte de Software | Unidad 1Código Creativo y Arte de Software | Unidad 1
Código Creativo y Arte de Software | Unidad 1
 
TataKelola dan KamSiber Kecerdasan Buatan v022.pdf
TataKelola dan KamSiber Kecerdasan Buatan v022.pdfTataKelola dan KamSiber Kecerdasan Buatan v022.pdf
TataKelola dan KamSiber Kecerdasan Buatan v022.pdf
 
Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions  for the students and aspirants of Chemistry12th.pptxOrganic Name Reactions  for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
 
KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...
KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...
KSHARA STURA .pptx---KSHARA KARMA THERAPY (CAUSTIC THERAPY)————IMP.OF KSHARA ...
 
“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...
“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...
“Oh GOSH! Reflecting on Hackteria's Collaborative Practices in a Global Do-It...
 
Incoming and Outgoing Shipments in 1 STEP Using Odoo 17
Incoming and Outgoing Shipments in 1 STEP Using Odoo 17Incoming and Outgoing Shipments in 1 STEP Using Odoo 17
Incoming and Outgoing Shipments in 1 STEP Using Odoo 17
 
A Critique of the Proposed National Education Policy Reform
A Critique of the Proposed National Education Policy ReformA Critique of the Proposed National Education Policy Reform
A Critique of the Proposed National Education Policy Reform
 
Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...
Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...
Kisan Call Centre - To harness potential of ICT in Agriculture by answer farm...
 
Introduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptxIntroduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptx
 
URLs and Routing in the Odoo 17 Website App
URLs and Routing in the Odoo 17 Website AppURLs and Routing in the Odoo 17 Website App
URLs and Routing in the Odoo 17 Website App
 
Introduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher EducationIntroduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher Education
 
BASLIQ CURRENT LOOKBOOK LOOKBOOK(1) (1).pdf
BASLIQ CURRENT LOOKBOOK  LOOKBOOK(1) (1).pdfBASLIQ CURRENT LOOKBOOK  LOOKBOOK(1) (1).pdf
BASLIQ CURRENT LOOKBOOK LOOKBOOK(1) (1).pdf
 
How to Make a Pirate ship Primary Education.pptx
How to Make a Pirate ship Primary Education.pptxHow to Make a Pirate ship Primary Education.pptx
How to Make a Pirate ship Primary Education.pptx
 
SOCIAL AND HISTORICAL CONTEXT - LFTVD.pptx
SOCIAL AND HISTORICAL CONTEXT - LFTVD.pptxSOCIAL AND HISTORICAL CONTEXT - LFTVD.pptx
SOCIAL AND HISTORICAL CONTEXT - LFTVD.pptx
 
The basics of sentences session 2pptx copy.pptx
The basics of sentences session 2pptx copy.pptxThe basics of sentences session 2pptx copy.pptx
The basics of sentences session 2pptx copy.pptx
 

Paper Electrophoresis: Separating Molecules by Charge

  • 1. PAPER ELECTROPHORESIS Presented by: A. Sireesha Bai M pharmacy first year Department of pharmaceutical analysis Santhiram college of pharmacy
  • 2.
  • 3. PRINCPLE: The charge carried by a molecule depends on the ph of the medium. Electrophoresis at low voltage is not usually to separate low molecular weight compounds because of diffusion, but it is easier to illustrate the relationship between charge and ph with amino acids than with protiens or other macromolecules.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10. APPLICATIONS: Serum analysis for diagnostic purpose is routinely carried about by paper electrophoresis. Muscle proteins, egg white proteins, milk proteins, and snake insect venom analysis done by this technique.