This document discusses the analytical technique of bioluminescence. It begins by defining bioluminescence as the production and emission of light by living organisms through a chemical reaction involving luciferin and luciferase. This reaction produces light energy. Bioluminescence assays can be used to measure cell proliferation, apoptosis, drug metabolism, and kinase activity. The document then explains the principle behind bioluminescence and provides steps for a bioluminescence procedure using cell lysis and a luminometer to measure light emission. Finally, some applications of bioluminescence are mentioned such as food testing, imaging, and medical research.