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Microbiological assay of antibiotics

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It's all about the microbiological assay of antibiotics and there has different type of microbiological assay of antibiotics.It's main purpose how to determine the potency of antibiotics.

Microbiological assay of antibiotics

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Presentation on
Microbiological Assay Of Antibiotics
Roll: 06,Semester:7th
Contents
• Antibiotics
• Biological Assay
• Microbiological Assay
• Microbiological Assay Of Antibiotics
• Types of Microbiological Assay Of Antibiotics
• Agar Diffusion Assay
Microbiological assay of antibiotics
Microbiological assay of antibiotics
Microbiological assay of antibiotics
Biological assay
•A bioassay is an analytical method to
determine concentration or potency of a
substance by its effect on living cells or
tissues.
•Experimental animals include mice, rats,
rabbits, cats, dogs etc.
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Microbiological assay of antibiotics

  • 1. Presentation on Microbiological Assay Of Antibiotics Roll: 06,Semester:7th
  • 2. Contents • Antibiotics • Biological Assay • Microbiological Assay • Microbiological Assay Of Antibiotics • Types of Microbiological Assay Of Antibiotics • Agar Diffusion Assay
  • 6. Biological assay •A bioassay is an analytical method to determine concentration or potency of a substance by its effect on living cells or tissues. •Experimental animals include mice, rats, rabbits, cats, dogs etc.
  • 7. Microbiological Assay •A type of biological assay is performed with micro-organisms. e.g, bacteria, yeasts and molds.
  • 8. Microbiological Assay Of Antibiotics •The microbiological assay of antibiotics is based upon a comparison of the inhibition of growth of micro-organisms by measured concentration of the antibiotics to be examined with that produced by known concentrations of a standard preparation of the antibiotic having a known activity.
  • 9. Types of Microbiological Assay Of Antibiotics A. The cylinder-plat(or cup-plate) method. B. The turbidimetric method.
  • 10. A. The cylinder-plate(or cup-plate) method. •The cylinder-plate method depends upon diffusion of the antibiotic from a vertical cylinder through a solidified agar layer in the petri dish or plate to and extend such that growth of the added micro- organism is prevented entirely in a zone around the cylinder containing a solution of antibiotic.
  • 13. B. Turbidimetric or Tube Assay method •This method depends upon the inhibition of growth of a microbial culture in a uniform solution of the antibiotic in a fluid medium that is favourable to its rapid growth in the absence of the antibiotic.
  • 15. Agar diffusion assay •The disk diffusion test, or agar diffusion test is a test of the antibiotic sensitivity of bacteria. •It uses antibiotic discs to test the extent to which bacteria are affected by those antibiotics.