laboratory techniques and laboratory safety

2. LET'S EXPLORE THE LAB!
YOUR TASK:
• FORM A GROUP AND SELECT YOUR LEADER!
• PICK THREE ITEMS FROM LABORATORY AND
MUST HAVE DIFFERENT SHAPES.
• DISCUSS THESE ITEMS TOGETHER WITH YOUR
GROUP AND SEARCH THEIR FUNCTION ONLINE.
(Write down your information in your journal)
• LET’S SHARE INFORMATION
• RETURN TO YOUR GROUP AND LET'S DISCUSS!

3. LET'S EXPLORE THE LAB!

4. WHAT ELSE CAN WE
FIND IN LAB?

5. WHAT ARE THESE MATERIALS?

6. WHAT ARE THESE MATERIALS?

7. WHAT ARE THESE MATERIALS?

8. LET’S WATCH A VIDEO
https://www.youtube.com/watch?v=gi3DeFY0cfw

9. WHAT IS HAZARD?
• HAZARD: CONDITION OR SET OF CIRCUMSTANCES THAT PRESENT A POTENTIAL FOR HARM
• TWO CATEGORIES :
• HEALTH HAZARDS: OCCUPATIONAL ILLNESSES
• SAFETY HAZARDS: PHYSICAL HARM, INJURIES

10. SAFETY PYRAMID

11. WHAT ARE THE HAZARDS INSIDE THE LABORATORY?

12. WHAT ARE THE HAZARDS INSIDE THE LABORATORY?
(CONT)

13. HOW DO WE PREVENT THE HAZARD OR HOW DO WE
MINIMIZE THE RISK?
• BY FOLLOWING THE LABORATORY SAFETY RULES.
• BY KNOWING THE MATERIAL OR THE EQUIPMENT YOU ARE USING.
• FOR CHEMICALS -> READ THE LABEL AND THE MATERIAL SAFETY DATA SHEETS (MSDS).
• FOR EQUIPMENTS -> KNOW THE TYPE OF THE EQUIPMENT AND FOLLOW THE STANDARD
OPERATING PROCEDURES (SOP).
• IDENTIFY THE RISK BY PERFORMING THE RISK ASSESSMENT.

14. LABORATORY SAFETY RULES
1. LABORATORY COAT REQUIRED WHEN ENTERING THE LAB.
2. ALWAYS TIE BACK LOOSE HAIR
3. ALWAYS FOLLOW THE TEACHER'S INSTRUCTIONS
4. BE NEAT AND ORGANIZED
5. REPORT ANY ACCIDENT RIGHT AWAY.
FOR EXAMPLE: ANYTHING SPLIT, ANYTHING BROKEN, ANYONE INJURED.
6. NO FOOD OR DRINKS DURING LABS
7. BE CAREFUL WITH ELECTRIC CORDS AND EQUIPMENT
8. BE CAREFUL WITH HOT ITEMS/ FLAMMABLES.
9. CLEAN UP AFTERWARDS.
- WIPE DOWN YOUR WORK AREA
-WASH YOUR HANDS BEFORE AND AFTER WORKING
- PUT THE CHAIRS BACK TO THE FIRST POSITION

15. CHEMICAL LABELS
• EVERY CONTAINER OF CHEMICALS IS LABELED BY THE
MANUFACTURER.
• THE LABEL WILL TELL YOU
• NAME OF CHEMICAL
• NAME, ADDRESS AND EMERGENCY PHONE NUMBER OF MANUFACTURER
• PHYSICAL AND HEALTH HAZARDS
• PRECAUTIONARY MEASURES
• FIRST-AID INSTRUCTIONS
• PROPER HANDLING/STORAGE INSTRUCTIONS

16. HEALTH HAZARD ON CHEMICAL LABELS
• CARCINOGEN
• HIGHLY TOXIC AGENT
• TOXIC AGENT
• REPRODUCTIVE HAZARD
• IRRITANT
• CORROSIVE
• SENSITIZER -> ALLERGIC REACTION.
• HEPATOTOXIN -> A SUBSTANCE TOXIC TO THE LIVER.
• NEPHROTOXIN -> POISONOUS OR DAMAGING TO THE KIDNEY.
• NEUROTOXIN -> SUBSTANCES THAT DAMAGE, DESTROY, OR IMPAIR THE FUNCTIONING OF
THE CENTRAL AND/OR PERIPHERAL NERVOUS SYSTEM.

17. COMMON SIGNAL
WORDS ON THE LABEL
• DANGER – CAN CAUSE
IMMEDIATE SERIOUS INJURY OR
DEATH
• WARNING – CAN CAUSE
POTENTIALLY SERIOUS INJURY OR
DEATH
• CAUTION – CAN CAUSE
POTENTIALLY MODERATE INJURY
SPECIAL SYMBOLS ON
THE LABEL
•Toxicity
•Corrosivity (acids and bases)
•Ignitability (flammable
solvents and
certain solids)
•Reactivity (sodium and
various water-reactive
reagents)

18. COLOR AND NUMBER CODED LABELS
Colors represent kind of hazard
• Red = fire
• Yellow = instability
• Blue = health
• black = specific hazard
& personal protection
Numbers show degree of hazard
• 0 = Minimal
• 1 = Slight
• 2 = Moderate
• 3 = Serious
• 4 = Severe
NFPA (National Fire Protection
Association)-type label

19. COLOR AND NUMBER CODED LABELS
Black = specific hazard
• OX = Oxidizer
• ACID = Acid
• ALK = Alkali
• COR = Corrosive
• W = Use no water
• Other symbols:

20. PROVIDES MORE DETAILED INFORMATION ABOUT A CHEMICAL,
INCLUDING
• COMPOSITION, INFORMATION ON INGREDIENTS (SECTION 2)
• HAZARDS IDENTIFICATION (SECTION 3)
• FIRST AID MEASURES (SECTION 4)
• ACCIDENTAL RELEASE MEASURES (SECTION 6)
• HANDLING AND STORAGE (SECTION 7)
• EXPOSURE CONTROLS, PERSONAL PROTECTION (SECTION 8)
• STABILITY AND REACTIVITY (SECTION 10)
• TOXICOLOGICAL INFORMATION (SECTION 11)

21. HOW TO HANDLE THE MATERIALS?
• DISPOSE OF CHEMICALS, BROKEN GLASS, AND
OTHER WASTE IN APPROVED CONTAINERS
• CLEAN UP BROKEN GLASS AND SPILLS
IMMEDIATELY
• KEEP THE LAB CLEAN AND NEAT
• STORE INCOMPATIBLE CHEMICALS IN SEPARATE
STORAGE AREAS

22. RISK ASSESSMENT
• WHAT IS A RISK ASSESSMENT?
• A RISK ASSESSMENT IS SIMPLY A CAREFUL EXAMINATION OF WHAT, IN YOUR WORK, COULD CAUSE
HARM TO PEOPLE, SO THAT YOU CAN WEIGH UP WHETHER YOU HAVE TAKEN ENOUGH PRECAUTIONS
OR SHOULD DO MORE TO PREVENT HARM.
• WHAT INFORMATION SHOULD A RISK ASSESSMENT INCLUDE ?
1. HAZARDS (WHAT COULD GO WRONG)
2. PEOPLE AT RISK (WHO MIGHT BE HARMED)
3. CONTROL MEASURES AT PRESENT (HAVE ENOUGH PRECAUTIONS BEEN TAKEN)
4. FURTHER ACTION REQUIRED (SHOULD MORE BE DONE)
5. RECORD DETAILS AND KEEP THE ASSESSMENT UNDER REVIEW

23. RISK ASSESSMENT MATRIX

24. RISK ASSESSMENT
Identify and evaluate existing risk controls
Who is at risk? Impact Likelihood Risk level exposure Preventive Action How we manage this risk.
Identify the risk
Risk Assessment Form
Analyze Risks and Evaluate Risks

25. PERSONAL PROTECTIVE EQUIPMENT (PPE)

27. PERSONAL PROTECTIVE EQUIPMENT (PPE)
• EYE PROTECTION
 SAFETY GLASSES – FLYING PARTICLES, CHEMICAL SPLASHES, DUST
 SPLASH GOGGLES – CORROSIVE LIQUIDS, SOLVENTS, POWDERS
 FACE SHIELDS – HIGH PRESSURE SYSTEMS
• RESPIRATORY PROTECTION – NORMALLY NOT NEEDED AT LUC
• SKIN AND BODY PROTECTION
 GLOVES – SEE THE MSDS
 APRONS AND LAB COATS – STRONG ACIDS AND BASES
 SHOES – ALWAYS WORN IN LAB, CLOSED TOE AND CLOSED HEEL
• HEARING PROTECTION – NORMALLY NOT NEEDED AT LUC

28. DO NOT
• USE DAMAGED GLASSWARE
• STORE CHEMICALS NEAR HEAT, SUNLIGHT, OR OTHER
SUBSTANCES WITH WHICH THEY MIGHT REACT
• STORE MATERIALS ON FLOORS OR OTHER PLACES WHERE
PEOPLE COULD TRIP OVER THEM
• LEAVE EQUIPMENT UNATTENDED WHEN ITS OPERATING
(UNLESS IT IS DESIGNED TO DO SO OR YOU HAVE AN SOP)
• PUT CUSTODIANS AND FELLOW WORKERS IN DANGER

29. • GET MEDICAL HELP IMMEDIATELY!
• INFORM YOUR LAB SUPERVISOR/LABORATORY STAFF OR THE
TEACHER.
• CHECK MSDS FOR FIRST-AID INSTRUCTIONS.
• SOME GENERAL GUIDELINES ARE
• EYES: FLUSH WITH WATER FOR 15 MINUTES
• INGESTION: FOLLOW LABEL AND MSDS INSTRUCTIONS
• SKIN CONTACT: STAND UNDER EMERGENCY SHOWER AND REMOVE
CONTAMINATED CLOTHING IMMEDIATELY
• INHALATION: GET TO FRESH AIR AND GET PROMPT MEDICAL ATTENTION

30. • LAB SIMULATION :
• HTTPS://WWW.LABSTER.COM/SIMULATIONS/LAB-SAFETY
Let’s play game !