The document discusses recommendations for setting up an IUI lab. It recommends that the lab have both a non-sterile and sterile area with adequate space to avoid compromising quality. Key considerations include basic infrastructure like air quality, lighting, temperature control and water supply. The document also recommends proper staffing with an embryologist, andrologist, nurses and counselor. Equipment, disposables, and high quality culture media are also essential to set up the IUI lab. Quality control, assurance and improvement should be prioritized. The lab design should have considerable input from embryologists and specialists to ensure success.

1. SETTING UPAN IUI LAB
Dr. RAHUL SEN PhD.
Consultant Embryologist
NEELKANTH FERTILITY HOSPITAL
JAIPUR

2. INTRODUCTION
• With the development of new techniques and procedures to
treat infertility, the perceptions and concerns of the medical
fraternity and public has evolved enormously.
• Setting up an ideal laboratory is the key to success in A.R.T.
• Before developing the basic design you know anticipated work
load, local building, practise permits and environmental factor’s.
• Every lab has its own unique features, any architect can design a
room but you must understand the type of work to be done.

3. • There are a number of ways to set up and
operate a successful assisted reproduction
technology (ART) Unit.
• Facilities in ART ranges from minimal to fully
equipped with additional space dedicated to
clinical care & research.
• There are many factors that go into making of a
good ART laboratory.

4. • HOW TO SETUP?
• WHEN, WHERE TO SETUP?
• IS THERE ANY GUIDELINE?
• WHAT ARE RECOMMENDATIONS ?

5. ESHRE
ASRM, SART, SRBT

7. Requirements for ART clinic- 2010 draft
• Space/ Location
• Non-sterile area
• Sterile area
• Proper Staffing Norms - TEAM
• Registration and Accreditation

8. Space
• A well designed ART clinic have a non-sterile and a strictly sterile area.
• Any space within the setup do no compromise with the quality of service.
• The guidelines do not specify the minimum areas required for each
purpose BUT it should be hassle-free and errorless.

9. ART LAB (ICMR)
NON-STERILE AREA
• A RECEPTION • RECOVERY ROOM
• A WAITING ROOM • SEMEN COLLECTION ROOM
• ROOM WITH PRIVACY • ROOM FOR IUI
• EXAMINATION ROOM
• STORE ROOM
• RECORD ROOM
• AUTOCLAVE ROOM

10. ART LAB ( ICMR)
STERILE AREA
• THE OPERATION THEATRE
• THE EMBRYOLOGY LABORATORY COMPLEX
• ROOM FOR STORAGE OF CRYOCANS
• SCRUB STATION

11. Team in ART Clinics’ ICMR- 2010
• Program coordinator/ Director
• Gyneacologist
• Embryologist
• Andrologist
• Counselor
• Nurses
• Housekeeping
• Helping Staff

12. Nursing
Patient care, doctor
communication,
Discipline
Housekeeping
Methods of hygiene
and management
Helping
Staff
Type of
work Andrologist
Lab, sperm
Quality
Embryologist
Gamete, Lab,
equipment,
Working Quality
Director
Construction,
Establishment,
Infrastructure,
type of facilities
Counselor
Type and quality of
patients,
methods of
counseling

13. Which clinics should be registered?
• Offers any treatment involving use/processing of gametes.
• That involves the use and creation of embryos outside the
body.
• The processing/storage of gametes or embryos.
• Research based activities.

14. levels of Infertility Clinic
(depending on their level of expertise).
• Level 1 (primary) infertility care units.
• Level 2 (secondary) infertility care units.
• Level 3 (tertiary) infertility care units.

15. Level 1 (primary) infertility care units.
• Investigation for cause of infertility.
• Treatment of minor anatomical defects.
• Detection and treatment of reproductive tract infections.
• Assessment of follicular growth and ovulation by USG.
• Cannot carry handling of gametes outside the body.

16. Level 2 (secondary) infertility care units.
Facilities for investigation of -
• Immunological tests for infertility.
• Semen analysis & Sperm function tests.
• Assessment of follicular growth and ovulation by TVS.
• Hysteroscopy, laparoscopy and TVS.
• Provision for semen collection, processing and
insemination for IUI.

17. Level 3 (tertiary) infertility care units.
Diagnostic procedures for male infertility
• Endocrine, hormone and blood related assays
• Detailed tests for sperm function and integrity analysis like using CASA.
Diagnostic procedures for female infertility
• Endocrine, hormone, blood & infertility related assays.
• Transvaginal sonography.
• Karyotyping
Therapeutic procedures
• Induction of ovulation
• All types of ART including ICSI
• Cryopreservation gametes and embryos

18. Requirements for IUI laboratory
• Equipment's
• Disposables
• Culture media
• Trained Professionals

19. Equipment's
• Imaging system- microscope.
• Controlled temperature system – warmers.
• Separation system - Centrifuge.
• Clean air system- Laminar air flow, room HEPA
filters.

20. Disposables
• The choice of plastic-ware plays a major role.
• Check sterility of plastic wares.
• Be user friendly with whatever disposable being used.

21. Culture media
• It is a medium which is required for growth and survival for
specific type of cell.
• Gametes have their own specific type of culture media to
remain viable in vitro.
• They widely vary in form of ingredients and technique of use.

22. –Location
–Air Quality
–Floor, walls, Ceiling
–Light
–Vermin Proofing
–Temperature
–Air Conditioning
–Water Supply
–Electricity
Basic infrastructure

23. Quality Control (QC),
Quality Assurance (QA),
Quality Improvement (QI)

24. Setting up a new unit is very much an
art, as is the practice of ART itself.
not recommend to be built by
administrators, engineers, or
architects
without considerable input from
experienced Infertility specialists &
Embryologists.

25. “ Your ART program is only as good as the lab which
supports it “