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Bio fertilizer
- 1. Welcome
- 2. Dr.Panjabrao Deshmukh Krishi Vidyapeeth akola College of agriculture ,Darwha Ta.Darwha Dis.Yawatmal Department of plant pathology Presented on Biofertilizer Presented by :- Shubham Sharad Nandane
- 3. Introduction Name of student :-Shubham Sharad Nandane Enrollment No:-OO-1776 Year:-Final year Bsc.Agri Semester:-8th Course No:-AEL-PATH-487 Course tittle:-biofertilizers Credit:-0+20 Department involved-pathology,Entomology,Economics and Extension Submitted by:-Shubham S.Nandane Submitted to:-Miss Shelki mam
- 4. Introduction What is a biofertilizer? Biofertilizers are the substance that contains microorganism's living or latent cells. Biofertilizers increase the nutrients of host plants when applied to their seeds, plant surface or soil by colonizing the rhizosphere of the plant. Biofertilizers are more cost- effective as compared to chemical fertilizers.
- 5. Importance of Biofertilizers Biofertilizers are important for the following reasons: Biofertilizers improve soil texture and yield of plants. They do not allow pathogens to flourish. They are eco-friendly and cost-effective. Biofertilizers protect the environment from pollutants since they are natural fertilizers. They destroy many harmful substances present in the soil that can cause plant diseases. Biofertilizers are proved to be effective even under semi- arid conditions.
- 7. Rhizobium: - For all legumes Rhizobium is applied as seed inoculant. Seedling root dip This method is used for transplanted crops. Two packets of the inoculant is mixed in 40 litres of water. The root portion of the seedlings required for an acre is dipped in the mixture for 5 to 10 minutes and then transplanted.
- 8. Preparation of Rhizobium Material required 1. HgCl2 solution(0.01%) 2. 75% alcohol 3. Sterile petridish 4. Conical flask 5. Glass rod 6. Sampel 7. Forceps 8. Razorblade 9. Sterile water 10.Loop 11.Spirit lamp 12.Measuring cylinder 13.Gas cylinder 14.Lignite powder
- 9. Composition of Congored Yeast extract mannitol medium Chemicals Mannitol K2HPO4 MgSO4.7H2O NaCl CaCO3 Yeast extract Distilled water Congo red Composition 10gm 0.5gm 0.2gm 0.1gm 3gm 1gm 1000 ml 15 ml of 1/400 aq solution
- 10. Procedure Isolation and purification 1. Uproot carefully vigorously growing legume crop Prefarably at flowering And bring the root sample with nodule in laboratory 2. East the root sample getly in tap water remove soil from it 3. Detach root nodule from it with the help of razorblade 4. Place the nodules under Petrosian with 1:1000 HgCl2 solution and agitate the module using needle 5. Transfer the nodule topetridiah containing 75% alcohol And agitate for 2 min 6. Then sterile and collect 2-3 sample of surface sterile nodule in test tube containing 1 ml sterile water 7. Crush the module in it 8. Add 1ml dilute exudes To a sterile petridish
- 11. Mother culture preparations 1. Pour PetrIdish with Solidifiable Congo red yeast extract mannitol agar Medium 45*c Mix the nodule exudes and Medium by rotating plates Gently 2. Allow the medium to solidify And incubate the plates At 28*C or at room temperature for 4-5 days 3. Transfer the growth from Rhizobial Colony to the Slants Of yeast extract mannitol agar Medium
- 12. CFU(colony forming unit) Mass multiplication of rhizobium japonicum 1. 2 lit of Congo red yeast extract mannitol agar solution is prepared 2. Then the prepared solution is then filled in 250 ml conical flask 3. Then the filled conical flask ask plugged with non absorbent cotton and wrapped with aluminium foil 4. The flask are placed in autoclave for about 40 min at 15 lbs at 121*C 5. The bacterial strain from mother culture are then transfer into congored yeast extract mannitol agar under aseptic condition with the help of laminar air flow 6. The prepared flask are plugged with cotton and placed in incubator at 30 *C for 15 days
- 13. Addition of carries material 1. 4 kg of lignite powder is used as carrier material (ratio- 1:2) 2. Thus this powder is taken in a tray 3. By the help of measuring cylinder the Congo red yeast extract mannitol solution is measure to a quantity of 1 litre 4. The measurevsolution is poured into the carries material (lignite powder )thoroughly 5. The powder is shade dried for 15 to 20 min
- 14. Mass multiplication of rhizobium japonicum Mixing of rhizobium culture into lignite powder
- 15. Packaging 1. Then the powder is Measure to a quantity of 500 gm Uaing digital weighing balance 2. 15 mm plastic covering are used to packaging The material 3. The filled Packers are then tightly Air sealed and then lebelled 4. The labeled packets Should contain the shelf life Period of the powder.Date of packaging,date of expire,trade name spp used Safely measured pracaution,made of application
- 16. Quality control 1. Cell no. At the time of manufacturing -10`89 carries within is days of manufacturing 2. Cell on at the time of expiry date -10`89 carries within 15 days before expiry date 3. Expiry date :- 6 month from the date of manufacture 4. Permissiable contamination level :no contamination at 10`8 dilution 5. pH:-6.0-7.5 6. Strain should be checked serologically 7. Carrier:-should passed through 150-212 microns IS sieve 8. Nodulation test should be above 20mg /g of glucose 9. Patent protection and prohibitive registration cost 10. Awareness training and education shortfalls 11. Lack multidisciplinary approach 12. Strategies to promote commercialization
- 17. The End Thanks for watching