Biostatistics and DNA analysis

1. Why “BIO”-statistics ?
Why DNA is analyzed ?
Eric, Po-Jen Wu (吳博仁)
Ph.D precandiate
The Institute of Basic Medical Sciences
National Cheng Kung University Medical college, Taiwan
E-mail: S58041103@mail.ncku.edu.tw or maple_823@hotmail.com

2. Statistics

3. Is Raymondhou handsome ?
Population Sample
Yes
Yes
Yes
Yes
Yes
Inference
Everyone thinks
Raymondhou is handsome?
Yes

4. Normal distribution
Sample
100%
(%)
●
●
● ●
●
●
●
Central Limit Theorem
Yes
Yes
Yes
Yes
YesYes
proportion of area

5. Standard deviation (SD)
Sample SD (The estimate of population SD)
Population SD
xi: finite data
μ: population mean
: sample mean
N: Number of population
n: number of sample
proportion of area

6. “BIO”-statistics
Analysis
• T test: Test for Differences in two groups
• ANOVA H P 1 m R N A
W
T
H
P
1
[0
4
]
H
P
1
[0
5
]
h
s
-H
P
1
0 .0
0 .5
1 .0
1 .5
HP1/rpl32ratio

7. Why DNA is analyzed ?

8. DNA
Nucleic acid and protein databases
• NCBI (National Center of Biotech Information) – GenBank for nucleotide sequences
http://www.ncbi.nlm.nih.gov/GenBank
• EBI (European Bioinformatics Institute) - EMBL (European Molecular Biology Laboratory)
http://www.ebi.ac.uk/
• DDBJ – DNA Data Bank of Japan
http://www.ddbj.nig.ac.jp/
Protein structure databases
• PDB – Protein Data Bank (protein 3D structure database)
http://www.rcsb.org/pdb/Welcome.do/

9. Demon