Zinsser Analytic Info & News 2018

1. Info & News
2018
CONTENT
Extraction of Micro-Organisms 1
Milk Sample Preparation 2
Viscous Liquid Blending 2
Powder Dispensing 3
Polyvials® DS 3
Automated SPE and Filtration 4
Measurements on LOGP 4
Liquid-Liquid Extraction 5
Pre-Formulation Studies 5
SOPHAS – A Genius Synthesis
Concept
6
Soil Contamination Analysis 6
Solutions for Substance
Libraries
7
Pipetting through Thick Seals 7
Minilab® 8
Liquid Scintillation Counter
LSC-8000
8
Polyvials® & Qualydrops® 9
Scintillation Cocktails 10
Low-Level Tritium Counting 10
EXTRACTION OF MICRO-ORGANISMS
Freeze-dried micro-organisms are
presented to the system in 24-position
deep-well plates and 4 ml of methanol
are pipetted to each well. To optimise
the extraction process, the sample
material is disrupted and mixed
with an Ultra-Turrax® mixer for three
seconds. The plate is then tilted and
the total volume of sample “slurry”
present is transferred with special
probes to 24-position filter plates.
Filtration occurs under vacuum or
positive pressure into a 24-position
deep-well receiving plate positioned
below. The workbench is configured
with stacker assemblies for 21 sample
plates, 21 filter plates and 21 receiving
plates therefore 21 sample plates (504
extractions in total) can be processed
in one run without user intervention.

2. LISSY® – MILK SAMPLE PREPARATION
LISSY® is a versatile system as typified by the wide range
of applications it can process. A further example is the pre-
paration of milk samples for QC testing, which involves a
variety of different experimental methods such as inhibitor
tests, or the detection of pathogens like feline leukaemia
and brucellosis. Boxes of raw milk samples from the dairies
containing samples in cartons or bottles (80 – 100 per box)
are delivered directly to the analysis laboratory for the
detection of residues or pathogens by ELISA. Through-
put demand can be as high as 1,000 samples per day from
each dairy. The milk samples are aspirated directly from
the sample bottles or milk cartons and pipetted into a
microtitre plate, including standards and controls. After
reagent addition and incubation, the microtitre plates are
read using a photometer. All samples are barcoded and
the work list for the run, either as an Excel® or a CSV file is
uploaded from the network at point of run. Each dairy can
supply a varying number of samples for analysis on a day-
to-day basis and priorities for preparation within the batch
can also vary. The software of LISSY® ensures that samples
are pipetted consecutively, that batches can be added on
the fly based on priority and that the start position within
a batch can be also determined based on priority with
transfer occurring to the corresponding position in the
microtitre plate. Our in-house team of designers, engineers
and software specialists enabled this complete solution for
the QC testing of milk samples.
VISCOUS LIQUID BLENDING & LUBRICANT DEVELOPMENT
BLENDA systems are used in industry to
speed up the development of new lubricants
for any application. Blenda 1 was designed for
medium size blends of oils and additives up to
500 ml, Blenda 2 for complex formulations
with inline viscosity adjustment. Both systems
can produce up to 40 blends per day.
BLENDA has been designed for the preparation
of complex blends of lubricants, oils and
other media (solids, liquids or compounds of
high viscosity).
Special pumps, liquid dispensing tools on a
2.5 m liquid handling workbench with two
independently operating arms and powerful
software allow high throughput blending
without compromise in precision or accuracy.
2

3. POWDER HANDLING WITH REDI-VARIX®
Our REDI® powder handling system can be used for any
workflow requiring powder handling in the range of 0.5 mg
to 2 g, such as the distribution of small quantities of solid
samples from compound libraries for screening tests, the
distribution of samples of freeze-dried plant material for
extraction or just the general pipetting of powders of all
types. The powder is aspirated from the source container
using a special powder handling probe and tip and is
dispensed gravimetrically to a set weight on an iterative
balance.
In addition, the initial source container can also be weighed
prior to and post aspiration as a pipetting check and the
destination vessel can also be weighed pre and post
dispensing. Depending on the powder properties, a
precision of ≤ 10% can be achieved, even at a low
dispense level of a 1 mg dispense with a throughput of
20 – 60 samples per hour. REDI-Varix® can be supplied in
conjunction with other system tools on a robotic platform –
as pure solid dosing only with no liquid handling or as an
integrated tool with liquid handling for sample preparation
or synthesis workflows. The integrity of the powder
samples is maintained with the use of inert gas shielding
for hygroscopic powders and efficient deionisation
modules for statically charged samples.
POLYVIALS® DS WITH TAMPER-EVIDENT CAPS
Our Polyvials® D are now also available
with tamper-evident caps!
They are manufactured under dust-
free conditions from a special poly-
ethylene for pharma packaging.
A coloured ring secures the screw cap.
When unscrewing the cap for the first
time, the colour ring will separate from
the cap. When the cap is unscrewed,
the ring will stay on the neck and can
be used for identification of the ingre-
dient. The caps are self-sealing and
are certified for airline transportation.
Rings and caps are available in multi-
ple colors. Volumes available are 15,
30, 60, 125, 250, 500 and 1000 ml.
3

4. AUTOMATED SPE AND FILTRATION SAMPLE PREPARATION FOR
PESTICIDE AND RESIDUE ANALYSIS
Speedy® works with nearly any kind of SPE cartridges, filt-
ration plates and extraction plates. With four independently
working pipetting channels (needle, disposable tip, piercing
needle), samples are transferred very fast from the sample
tubes to the extraction cartridges. With the integrated six-
way valve, Speedy® can equilibrate and wash the extraction
matrix with high volume of up to six different solvents very
quickly. The buffers are stored below or beside the working
area to save valuable place.
Speedy® eluates the samples with vacuum or with adjust-
able positive pressure and pressure control to prevent fatal
drying of sensitive matrices like affinity columns which are
used in the analytics of mycotoxines, steroides or anti-
biotics in food, blood and urine.
Speedy® can also handle higher sample volumes, which are
common in food and pesticide analysis like the QuEChERS
method. The integrated robotic arm can transport racks,
vials, extraction plates, filtration plates, microtitre plates or
any kind of tools.
Speedy is controlled via the flexible and easy-touse
software WinLISSY®. WinLISSY® gives you access to all
method steps and to all liquid handling parameters, which
is a very powerful tool for the development, adaption and
optimization of methods.
MEASUREMENTS ON LOGP, LOGD AND KINETIC SOLUBILITY
ACCELERATES EARLY DRUG DISCOVERY RESEARCH
Current medicinal chemistry is under pressure to accelerate
the outcome of research results. Early determination of as
much data as possible from new potential drugs is crucial
to fulfill this difficult target. A broad knowledge of the
properties of all compounds involved in a research project
helps the scientists to make the right decision which lead
structures they should follow. Zinsser Analytic has
developed a sample preparation system that can prepare a
complete set of HPLC samples for an exact measurement
for kinetic solubility, logP and logD just using a tiny amount
of 0.3 μmol. The system, which was developed for a major
pharmaceutical company, just needs 30 μl of 10 mM DMSO
solution per compound provided in Remp® tubes in a 384-
well format. High precision liquid handling allows the fully
automated preparation of two HPLC samples for all three
measurements (double determination). In the case of logD,
the partition coefficient is determined from the equilibra-
tion between buffer (pH 7.4) and n-octanol by separating
the two phases in a centrifuge and taking aliquots from
both phases. HPLC samples ready for direct analysis can be
prepared for 96 different compounds in 16 h. The high
throughput makes it possible to automatically measure
logP, logD and kinetic solubility for all compounds involved
in a project and to screen entire compound collections.
4

5. LIQUID-LIQUID EXTRACTION OF SYNTHETIC SUBSTANCES
Our LISSY® system allows the full
automation of the isolation of a sub-
stance of interest through liquid liquid
extraction. In this case, the substance
to be isolated is present on the walls of
30 ml sample tubes that have previous-
ly been evaporated to dryness. These
tubes are accommodated on the work-
bench in racks with an 8x12 format. To
solubilise the dried components in an
organic phase, a limited volume only
(approx. 1 ml) can be used. The organic
phase is therefore added to these tubes
using a pipetting probe that sprays
the walls of the tube with the solvent
as it is dispensed. The pipetting probe
also adds the aqueous phase via a
second channel and, depending on the
workflow, further organic solvent can
also be added with the use of a six-way
valve assembly. The total amount of
aqueous and organic phase is 10 ml.
Full mixing of the phases and hence ex-
traction of the component of interest is
achieved using an adjustable eight-fold
overhead stirrer (Ultra-Turrax® stirrer
with eight impellers) with the extraction
step and the formation of the interface
between the two phases taking only a
few seconds. The final step is the trans-
fer of the phase of choice (organic) to
6 ml filter cartridges for sample filtra-
tion under positive pressure from
above with the substance of interest
collected in receiving vials below.
CRISSY® AND SUSY FOR PRE-FORMULATION STUDIES
Good solubility and appropriate logD
values are not the only important prop-
erties of potential drug compounds.
Crystal structures and polymorphism of
these compounds alone or in combina-
tion with the excipients in pharmaceuti-
cal formulations have a huge impact on
their stability and durability. Together
with our worldwide customers in
pharmaceutical R&D and institutes we
have developed a variety of bench-top
automation systems for the preparation
of formulations and subsequent crystal-
lization experiments. These automated
workflows allow a systematic formula-
tion screening and polymorphism
studies under reproducible conditions.
Based on the requirements of our
customers, our experience and the use
of proven tools from our toolbox, we
design and build an optimized system
to fulfill your needs from the first day in
use.
CRISSY® for polymorph screening using
special reactor blocks for direct analysis
of the crystals in an XRPD X-ray spectro-
meter.
SuSy for solubility testing with specially
developed stirrer units, pH monitoring
and much more.
Formula X® for formulation studies, with
the capability to optimize existing for-
mulations or to create new ones.
5

6. SOPHAS® – A GENIUS SYNTHESIS CONCEPT FOR CATALYSTS
In today’s rapidly changing market
place, catalyst researchers are forced
to produce results to an ever-higher
standard within a limited time scale.
The key to achieving this goal is to
utilise high-throughput techniques
to efficiently generate the necessary
experimental data quickly, thus con-
trolling expenditure. Zinsser Analytic
offers a wide range of tools for the
high throughput development of cata-
lysts. These range from manual parallel
systems through to a real robotic
“research catalyst factory”. Available
features include the complete work-
flow of precursor preparation, synthe-
sis, impregnation, co-precipitation,
calcinations, sieving, tablet forming
etc. Smaller, less expensive systems
are available as dedicated work-
stations for a portion of the workflow.
For parallel scale up in the range of
100 - 300 ml systems have been
engineered. Maximum flexibility is
achieved on all our systems, which are
controlled by our proprietary Zinsser
Analytic software. Methods can be
simply generated and communication
with existing databases and informa-
tion systems is made easy.
Sophas with Reflux reactors Sophas CAT HT for high throughput synthesis
Desyre-reactor block
SAMPLE PREPARATION FOR SOIL CONTAMINATION ANALYSIS
Sample preparation for environmental
monitoring of soils (MOSH, PAH, PAK,
PCB, AOX, EOX) results in many tests
per original sample and it is time-
consuming, tedious manual work and
the technicians are exposed to hazard-
ous solvent vapors like n-hexane or
cyclohexane. The samples have to be
prepared and analyzed reliably and
without delay. MOPS works up more
than 70 soil samples per day for the
automatic analysis of contamination
by mineral oils, hydrocarbons and
PAK/PCB/EOX. The system is loaded
with the soil samples and, after the
fully automatic preparation and ex-
traction procedure, produces ready
to use crimped sample GC/LC vials.
The methods are according to the
legal regulations of the original
manual procedures but without the
human errors. The procedures include
precise liquid handling for ISTD addi-
tion, shaking and ultrasonic agitation,
centrifugation, SPE, evaporation, vial
capping/crimping and more. For a
higher throughput, the system is
equipped with two independently
working robotic arms on one platform.
Both arms are assigned to certain
tasks but they can also work together,
e.g. one arm moves the vials to the
capping station while the other adds
internal standard. MOPS will be the
new standard in environmental
analysis laboratories.
6

7. SOLUTIONS FOR SUBSTANCE LIBRARIES
Huge numbers of valuable compounds are taken from
compound collections every day to prepare solutions which
are transferred to test plates and vials for biological assays.
Zinsser Analytic has delivered many systems to almost all
major pharmaceutical companies and institutes, which en-
sure fast, efficient and compound saving handling.
proForma® systems automate tare weighing, weighing,
capping, barcode reading, sorting and formatting of
sample vials. Samples are ready to store in compound
collections and proForma’s software can be integrated
into any compound logistic database. Smaller systems can
handle hundreds of bottles while production type systems
for a throughput of thousands of bottles per day are
usually equipped with multiple scales, cap feeders and
parallel capping stations.
Librarian a newly developed powder handling system
which can withdraw very small volumes (0,5 mg - 50 mg)
of sample material from storage vials for screening tasks –
even from the corners of the vial bottoms – with highest
precision, and automatically weigh in into the dissolution
vials.
MOSS systems are specialized in the preparation of
compound solutions with exact concentrations. In most
cases compounds are dissolved in DMSO which is being
accelerated by stirring, shaking and ultrasonic agitation.
Inert atmosphere (Ar/N2) can be provided. Optionally,
a camera and image interpretation software can be
implemented to insure complete dissolution and to take
logical decisions for an automated subsequent processing
(i.e. prolonged agitating or adding more solvent).
PIPETTING THROUGH THICK SEALS ...
Is it possible to access containers which are sealed with
thick rubber stoppers with a pipetting needle? Yes! We
have solved this challenge by redesigning our robot to use
commercially available injection needles instead of normal
disposable tips for pipetting. Using this new design along
with our clever software, we have produced systems for
the quality control of injection medium, where samples are
removed from the finished, stoppered injection vials and
cartridges prior to QC analysis and radioactive tracer is
then subsequently added to closed vials. The new design
ensures contamination free sampling and precise and
reproducible pipetting.
7

8. MINILAB®
For more than 20 years, the Minilab® – our semi-micro
glassware kit – has been used with great success by many
schools and research centres to conduct chemistry experi-
ments and specialized chemistry training. A new textbook
by Prof. Michael Schallies with information ranging from
basic chemistry functions to organic syntheses is now
available. The detailed experiment instructions contain
the corresponding references and safety regulations. The
Minilab® is characterized by its unique design: All parts are
easily combinable with the PTFE threaded couplings and
can be attached to a stainless steel plate using magnetic
clamps or clipped onto a tripod.
8
LIQUID SCINTILLATION COUNTER ACCUFLEX LSC-8000
AccuFLEX LSC-8000 of Hitachi
ensures accuracy and flexibility for
any basic liquid scintillation measure-
ment in research and environmental
laboratories. Quantification of all the
beta emitters (with optional alpha/
beta separation) is performed using
4000 channels and optimized energy
ranges. Pre-set counting windows or
Auto Isotope Selection offers any re-
sult units in counts, cpm, dpm or Bq.
LSC-8000 can be used with either
20 ml, 8 ml, 6 ml glass or plastic vials
or micro tubes, with a 408 samples
loading capacity in its racks-based
samples changer.
Accurate measurements are provided
with External Standard Channel Ratio,
Self-Constant Channel Ratio methods
and other advanced features such
as Chemical Luminescence, Color
Quenching Corrections and Anti-Static
Function.
Data reliability is fully supported by
a System Performance Evaluation,
including History Review and Security
locks, in a GLP mode, with 21 CFR Part
11 included as a standard feature.
Windows-based user friendly software
and built-in PC with touch panel color
LCD and various output interfaces
gives all the comfort required on a
modern, easy-to-use standard liquid
scintillation counter

9. POLYVIALS® & QUALYDROPS®
9
POLYVIALS® & QUALYDROPS®
Our Polyvials® V range from 4 to 250 ml with self-sealing,
leak-proof screw caps and are widely used for packaging
valuable reagents in the diagnostic industry. They are man-
ufactured from selected polyethylene and polypropylene in
cleanroom facilities (Class 10.000, ISO 7, USTD 209E). On-
line quality control assures consistent high quality.
Polyvials® V
These vials have set a standard in diagnostic packaging.
Their special design allows easy filling and retrieval of re-
agents. The caps allow gastight sealing even under extreme
temperatures and pressure. This makes them safe for air
transportation. The vials are supplied in natural (opaque) or
in lightproof black polyethylene. The caps are made from
polypropylene and are available in various colors.
Polyvials® D & DS
They are thick-wall packaging vials with wide caps for dry
and liquid reagents and media. They are supplied in sizes
ranging from 15 ml to 1.000 ml volume in natural (opaque)
virgin polyethylene. They are suitable for gamma or elec-
tron beam sterilization.
Polyvials® DS
These are Polyvials® D with special tamper-evident caps.
After unscrewing the vial, a color ring stays on the neck of
the vial for identification. Also, coloured caps are available
on request.
Micro-Polyvials® 1.5 ml
They are ideal for shipping and storing small quantities.
The conical bottom allows complete retrieval of the con-
tent. The unique screw caps seal without any additionally
inserted septum or “O”-ring.
Qualydrops® Dropper Bottles
They have a special dropper insert which delivers preci-
se volumes, i.e. for water the drop size is 40 μl ± 5%. The
bottles are precision molded from LDPE. They are available
in the standard sizes of 3 ml, 5 ml, 10 ml, 20 ml and 30 ml
bottles in white. Natural and black vials can be manufac-
tured on request.
9

10. ULTRA-LOW-LEVEL TRITIUM COUNTING
The new LB-7 of Hitachi LSC-LB is the only real low-level
scintillation counter. The detection limit is better than 0,1 Bq
per liter of water. The counter uses 145 ml vials which allow
to count seven times the volume per sample compared to
other low volume systems. Tritium counting efficiency with
145 ml vials is better than 25% for 70 ml of sample and
70 ml of our special low level cocktail „Quicksafe 400“.
1010
SCINTILLATION COCKTAILS
For 42 years Zinsser Analytic has been
supplying scintillators and consum-
ables for liquid scintillation counting.
Zinsser has had a great influence on
the development of scintillation cock-
tails and sample vials, as with our first
cocktail Unisolve 1 and our well proven
Polyvials which have a unique profile
for increased stability and a superior
cap sealing system which guarantees
a gas tight sealing, even at fluctuating
pressure or temperature and which
are used by researchers around the
world. Since May 2012, we have been
offering Supersolve-X, a newly
developed safe scintillator which
stands for high sample capacity –
even for alkaline solutions – and
outstanding efficiency. Contact us for
a data sheet and sample for testing!

11. 1111

12. ZINSSER ANALYTIC GMBH
Contact Europe
Eschborner Landstr. 135
60489 Frankfurt / a.M., Germany
Tel: +49 69 78910 60, Fax: +49 69 789106 80
Contact Americas
19145 Parthenia Street, Suite C
Northridge, CA 91324, USA
Tel: +1 818 341 2906, Fax: +1 818 341 2927
info.zinsser-analytic@gardnerdenver.com
Headquarters
Gardner Denver Thomas GmbH
Livry-Gargan-Str. 10
82256 Fürstenfeldbruck, Germany
Tel: +49 8141 2280 0, Fax: +49 8141 8892136
thomas.de@gardnerdenver.com
www.zinsser-analytic.com
www.gd-thomas.com