INTip solid phase extraction
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INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip. This technology enables INTip solid phase extraction for easy sample preparation. The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.
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INTip solid phase extraction
- 2. 1998 - Conceptualized byWilliam Brewer, PhD 2000 – US PATENT NO. 6,566,145 2007 – DPXTechnologies Established 2008 – Global tips sales + additional patents 2013 – US PATENT NO. 9,733,169 2015 – Collaboration 2016 – Patent Filed forTip-on-TipTechnology 2018 – New 12,000+ sq. ft. Facility 2019 – DPX Rebrand ABOUT US Solving problems with chemistry is the core of what we do. Our vision is to empower scientists by offering versatile products that can integrate with any workflow or method.
- 3. • INTip Sample Preparation Products • INTip SPE • Bind-Wash-Elute • Cleanup • DPX + SALLE • Sorbent Chemistry Options and Tips Styles • Applications Overview • Urine • Blood • Oral Fluid • Summary INTip™ Purification Our proprietary and patented pipette tip technology provides solutions for a variety of applications.
- 4. INTip™ Solid Phase Extraction Powered by Dispersive Pipette XTRaction™ Technology INTip™ Filtration Powered by Tip-on-Tip™ Technology
- 5. INTip Solid Phase Extraction INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip. This technology enables INTip solid phase extraction for easy sample preparation. The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries. You can seamlessly integrate XTR tips with any workflow or method. Pipette tips are available in manual, semi-automated or fully automated formats with a variety of sorbent chemistries.
- 6. • Higher analyte recoveries • Unparalleled ease of use • Seamless integration with most pipetting systems • High throughput • Rapid extraction times • Sorbent chemistry customization • High reproducibility Barrier Disperser Loose sorbent Customizable Frit BenefitsXTR Tip Anatomy Delivering Purification Excellence 6.
- 7. SOLID PHASE EXTRACTION IN A TIP Condition Bind Wash Elute Elute analyte of interest Disperser ensures turbulent mixing of loose sorbent Aspirate and dispense steps, bind sample Interferents wash off Delivering Purification Excellence 7.
- 8. Simple aspirate and dispense steps mix sample solution with sorbent.
- 9. Aspirate Sample Mix Solution Dispense Analyte Interferents bind to sorbent CLEANUP Dispersive Pipette XTRaction supports consistent, high quality data to ensure long term robustness of mass spectrometers. • No vacuum manifold required • Easily remove matrix interferences for cleaner extracts Delivering Purification Excellence 9.
- 10. DPX + SALLE XTR tips can house sorbents likes Weak Anion Exchange (WAX) or Reverse Phase (RP) for cleanup and the salt (S) necessary for SALLE (Salting-out Assisted Liquid-Liquid Extraction). This methodology is ideal for applications that require acetonitrile for protein precipitation and are focused on hydrophobic analytes. • WAX-S • RP-S Delivering Purification Excellence 10. Aspirate/ dispense steps mix with air Phase Separation Clean Extract Transfer upper acetonitrile layer
- 11. Chemistry Options and Tip Styles INTip™ SPE SORBENT XTR Tips mXTR Tips (micro-elution) (Hamilton & Integra style only) • WAX • WCX • SCX • SAX • C18 Hamilton (300 mL/1mL) Tecan (200 mL/1mL) Eppendorf epT.I.P.S. (1 mL) Integra (300 mL / 1250 mL) Gerstel (1 mL) Universal (1 mL/5 mL) STYLE • RP • Silica • Mixed Mode • HybridSPE® • DPX + SALLE Delivering Purification ExcellenceDelivering Purification Excellence 11.
- 12. HybridSPE® - Phospholipid removal • DPX partnership with Sigma/Supelco • Ideal for serum cleanup Delivering Purification Excellence 12.
- 13. XTR Tips • Manufactured to order per custom specifications • Hold 1 – 100 mg of sorbent • Sorbent chemistries in a range of phases • Seamless integration with any workflow or method • Available in manual, semi- automated or fully automated formats μXTR Tips • Proprietary design that utilizes a unique low-retention frit • Minimal elution volumes for maximum concentration effects • Available with 1-3 mg of sorbent • Elution volumes as low as 25 μL • Eliminate time-consuming solvent evaporation steps • Available in Hamilton, or Integra 300 mL formats. Delivering Purification Excellence 13.
- 14. Compatibility XTR tips can be used manually with single or multi-channel pipettors, or on automated liquid handling systems. Our team of application scientists supports custom method development to help you seamlessly integrate our products into your work-flow. Delivering Purification ExcellenceDelivering Purification Excellence 14.
- 15. Applications
- 16. Matrices Analytes Therapeutic/Abused Drugs Vitamin D Catecholamines Steroids EtG/EtS Antibiotics Pesticides Hormones Cannabinoids Urine Oral Fluid Blood Serum Plasma Food Extracts Plant Extracts Tissue DIVERSITY OF APPLICATIONS We are going to highlight a few methods in this presentation. Delivering Purification Excellence 16.
- 17. Condition 30% MeOH Bind Hydrolyzed Urine Wash Water Elute 1% FA MeOH Dilute Water Therapeutic/Abused Drugs Analysis XTR tips XTR tips 1 mL tips Delivering Purification Excellence 17.
- 18. 0 10 20 30 40 50 60 70 80 90 100 Analyte Recovery - Urine Delivering Purification Excellence 18.
- 19. Condition MeOH Wash buffer 0.2 M NH4Cl pH 8.5 Bind 300 mL urine 600 mL complex buffer Wash Elute 300 mL 1 M Formic acid 10% MeOH Analytes are retained via p-p interactions Delivering Purification Excellence 19. Analysis of Catecholamines in Urine
- 20. 96% 98% 81% 97% 97% -39% -9% -1% -14% -11% Recovery Matrix Effects Catecholamines Recovery and matrix effects Delivering Purification Excellence 20.
- 21. DPX METHOD DEVELOPMENT Analyte(s) of Interest? Ex: Basic Small Molecule Identify DPX high quality sorbent Ex: Strong Cation Exchange(SCX) Sample Volume/Matrix? Ex: 100 mL plasma + 100 mL buffer Identify sorbent amount Ex: 1 mg Concentration dilution factor? Ex: 4 fold concentration Solvent evaporation? No Identify DPX Product mElution dSPE Tip with 1 mg SCX Identify Elution Volume 25 mL Delivering Purification Excellence 21.
- 22. Copy Right © All Right Reserved. DPX Technologies Dispersive Pipette XTRaction™ - trademark DPX Technologies Tip-on-Tip™ - trademark DPX Technologies INTip™ - trademark DPX Technologies
Editor's Notes
- The alternative to the bind wash elute is a clean-up method. Here, the matrix is bound to the resin, leaving a clean solution containing the analytes of interest behind. Two of the most common DPX Clean Up tips are the Supelco Hybrid phospholipid removal tips and WAX-S tips, the tips of discussion in our two posters this year at MSACL.
- The alternative to the bind wash elute is a clean-up method. Here, the matrix is bound to the resin, leaving a clean solution containing the analytes of interest behind. Two of the most common DPX Clean Up tips are the Supelco Hybrid phospholipid removal tips and WAX-S tips, the tips of discussion in our two posters this year at MSACL.
- Urine