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Indian Council of Agricultural Research
ICAR Research Complex for NEH Region
Tripura Centre, P.O. Lembucherra-799210
Acknowledgement: We sincerely thank to Dr. Narendra Prakash, Director, ICAR RC
NEH Region for his guidance and to all who contributed directly or indirectly for pre-
paring this manuscript.
Disclaimer: Some contents and photos are taken from difference sources to make the
topic more elaborative for farmer’s easy understanding and for welfare of farmers.
Sources/ person are hereby well acknowledged .
Prepared & Designed by:
Dr. Biswajit Das, Principal Scientist, Horticulture, ICAR RC, Tripura Centre
Published by :
The Joint Director, ICAR RC for NEH, Tripura Centre
For more details please contact:
Dr. B. K. Kanpal
Joint Director
ICAR Research Complex for NEH Region,
Tripura Centre, Lembucherra, Tripura (West) - 799210
E-mail : jd.tripura@Icar.gov.in
Dr. Biswajit Das
Principal Scientist, Horticulture
ICAR RC, Tripura Centre
E-mail: biswajitsom_dr@yahoo.co.in
Copyright ©: ICAR-NEH Region, Tripura Centre.
Year 2018 Publication No. 56
Plug Nursery
for quality planting material
production
Plugs for vegetable seedlings
Vegetable portray nursery can be raised under normal net house or naturally venti-
lated polyhouses. However, advanced Hi-tech polyhouses with capacity of produc-
ing 5-10 lakhs seedling per batch may be established depending upon the require-
ment. Portrays with grownup seedling are as such transported even to long distance
areas for transplanting.
Portray nursery of ginger
Ginger rhizomes are sprouted by placing on sands bed and when buds are bulged all
the rhizomes are taken out of the sand deb. Rhizomes are cut into small pieces each
segment having one bulged bud. Each piece is put into portrays and filled with soil-
less media such as cocopit + vermicompost. After about 2 months plants are ready
for transplanting.
Transplant the Plug Plants Carefully
Plug plants earth cone is loosened by slight tapping and care is taken that earth and
root zone remain moistened, but conical shape of the earth does not break and re-
mains intact when taken out from the tray cells for transplanting. Vegetable plugs
are transplanted directly into the field by making hole at crop specific spacing.
Ornamental/flower plants are planted in pots which are filled with moistened pot
mix and a hole is made slightly bigger in size than the plug plant earth cone at the
center. Thus, raising flower plants or vegetable crops from plug plants improved the
Seed Germination 100% & Uniform Seedling Growth
Plug Plants: No transplant shock & plant death
Biswajit Das
H. Lembisana Devi
B. K. Kandpal
Points to be noted
1. Rooting media should be light and soil borne diseases-insects free .
2. No water stagnation inside the tray cells.
3. Temperature should be 20-30 0
C for better seed germination.
4. Seedling should be over mature to avoid extra growth of roots.
5. Prior to transplanting hardening and acclimatization is essential.
6. Seedlings may be affected by rotting at the color region due to Damping off
disease, if rooting media or seeds are not treated. Drench the media with
carbendazim and mancozeb mixture or metalyxil (15-20g/10L water).
7. Appropriate insecticides may be sprayed for insects such as monocrotophos
or metasystox (1.5 ml/L water) for leaf minor (white tunnels along the veins
in the leaf lamina), thiamethoxame (1ml/L water) for whiteflies and neem
oil (2-3ml/L water) or chlorpyrifos/dimethoate/quinalphos (1ml/L water) for
any leaf damaging insects.
8. Plugtrays/portrays should be handle with care and reused.
9. More than one seedling may be raised per cell of portrays for ease of trans-
portation in case of transplanted vegetable crops and to minimize cost, how-
ever, each seedling is separated with care at the time of transplanting.
Quality
Seedlings
Determine
Quality
of
Produce
Quality planting material is the basic requirement for production of high
value flowers and vegetables. It is not possible for each gardener or farmer
to raise nurseries for all the plants they are interested to grow. In the recent
years, plug plant nurseries have become very popular in India for high value
flower and vegetable crops raised under protected or open conditions.
Continue…..
What is Plug nursery?
Seedlings or nursery plants are grown as a separate unit in modular
plugtrays/portrays with separate cells/conical cavities containing growing
media and transplanted with intact root soil.
Advantages
a) Costly seeds of high value crops or hybrids and portrays ensure germina-
tion of each seed, b) Growing of such plants namely lilium or tulip etc.
whose bulbs can not be produced under Tripura climate, c) Management
practices can be employed for better and uniform growth of plant and root,
c) Less affected by disease-insects and weeds, d) Long distance transport
without any disturbances, e) Transplanted to field or container in any season
without any shock, and f) Maximum field survivability.
Treat the Rooting Media
Solarization by exposing the soil under sun and airtight covering the soil
with polyethylene for 5-6weeks. So that temperature inside increases and
organism gets killed without oxygen. Drenching the soil mix with formalin
2-5% solution (200-250ml/10 L) upto a depth of 15-20 cm @ 4-5 L/m2
soil
surface and immediately covered with black polyethylene sheet for 6-7
days. The mix is raked after removing the polyethylene cover and is left
exposed for at least 4-5 days. Soil may be drenched with solution of car-
bendazim or captan (3-4g/L) @ 1-2L/m2
soil surface. Phorate powder (5g/
m2
mixture) is also applied in dried soils.
Fill the Portrays with Rooting Media
Approximately 1.0-2 Kg of the coco peat medium is filled in each portray
depending upon the size. Each cells of the tray are filled with the mix upto
1 cm below the rim and slightly pre-moistened by sprinkling water on the
trays and top surface of the mix of each cells are leveled by a piece of wood
frame or rod.
Treat the Seeds
Seed treatment is essential as prophylactic measure. Commercially availa-
ble seeds are pretreated with fungicides. However, it is beneficial to treat
seeds prior to sowing with captan or carbendazim (@2-3g/L water/kg seed).
Biofungicides such as Pseudomanas fluorescens (10g/Kg seeds) or Tricho-
derma viride (4g/kg seed). Seeds may be mixed with Azospirillum @ 200g/
kg seed and are shade dried for half an hour.
Seed Rate
Requirement of seed per
hectare is less for portray
nursery raising system in
comparison to open bed
nursery raising of vegeta-
bles and flowers.
Sow the Seeds in Portrays
A small hole (1-2 cm deep) is made at the centre of each cell by a thin stick. Seed
is put into each hole and covered with fine potting mix by slightly pressing with
thumb tip, so that, no air space is left around the seed. The trays may be covered
with dry grass/hay straw and is removed just after germination. Soil is kept mois-
tened by gently sprinkling water with rose cane. Germination takes place within 7-9
days after sowing. Selective weeding is done and at 3-4 week stage water soluble
fertilizer as per requirement is given along with irrigation. At 6-8 week stage plug
trays containing seedlings (8-12 cm height, 4-6 leaf stage or even more in case of
flowering plants) are ready for transportation and transplanting. Prior to transporta-
tion or field planting hardening for 3-5 days is beneficial for better survival.
Portrays/plugs for Chrysanthemum
Plants are cut above 7-10 cm from ground level after flowering when flow-
ering is over. Several suckers arise laterally at the base of the plant. Suckers
are separated and planted plug cells 10-15 cm. Soft stem cuttings are taken
from healthy stock plants from end of September-October or February to
middle of June. Five to ten cm long cuttings are made by shearing the basal
leaves. For enhancing rooting these cuttings can be treated with 500 ppm
NAA + 100-200 ppm IBA for 5-10 sec. Treated cuttings are planting in plug
trays containing soilless media.
Plugs for Rose plants
‘Stenting’ is an advanced and quickest method of rose propagation. In this
method, buds of suitable cultivars are budded on local unrooted rootstocks
and kept in IBA (500-1000 ppm) for 15-20 sec. The budded unrooted root-
stocks cuttings are kept for rooting under intermittent mist in plug trays.
Semi hard wood cuttings of 6-8 mm diameter and 10-20 cm long are col-
lected from actively growing mother plants. Cuttings are inserted 5-10 cm
into the rooting media. Rooting and bud union takes place simultaneously.
Otherwise, rooted cuttings of Rosa indica, Rdward rose or Rosa multiflora
or any other local hardy roses may be raised in plug trays and later on bud-
ding is done.
Plugs for Gerbera
Gerbera may be propagated by seeds, separation of crown clumps or suck-
ers and micro-propagated plantlets which are specially raised in plug trays.
Gerbera seeds are very minute and lose viability, if sowing is delayed. Each
seed is carefully sown in individual cell containing pre-moistened pot mix
of peat: perlite (1:1) and covered with a thin layer of vermiculite. Trays are
kept in high humidity germination chamber at RH 90-95% and temperature
20-24 0
C. Germination starts after 7-14 days and then all the seedling trays
are shifted to polyhouse. Suckers/clumps are separated from the mother
plant during June-July and planting into the plug trays. Tissue culture raised
plantlets are planted into plug trays containing perlite: sphagnum moss (1:1)
in the hardening chamber and after acclimatization plants are transported or
planted in the field.
Plugs for Anthurium
Division of roots, separation of suckers, seeds and micro-propagated plantlets are
methods of Anthurium multiplication. Offshoots arising laterally from the base of
the main stem of mother stock are separated along with portion of arial roots.
Around 14-15 offshoots may be collected from a single plant per year. These off
shoots are planted in the plug trays in individual cells containing peat: pine bark/
coconut husk: perlite (2:2:1). Trays are kept in shade house under indirect sunlight
and moist and humid condition. Tissue culture raised plantlets are transferred to
small plug trays containing vermicompost: sand (1:3) or sand: charcoal (3:1) or
pear: perlite: sand (1:1:1) in the hardening chamber for hardening.
Requirements
The essential requirements are Plugtrays/Portrays of different Nos. and cell
dimensions. Cell number per tray may vary from 12 to 512 depending up-
on the requirement and plant type and each cell volume may vary from 3 to
300 cc. Normally 98 cells (54 x 27cm) of 3.5 cm diameter and 0.8mm
thickness Portrays are made of UV stabilized PVC or biodegradable plas-
tics with thickness of 0.85 -1.5 mm.
Protected structures
Shade house/net house/polytunnels/naturally ventilated poly house with
well ventilation and irrigation facility. In Hi-tech polyhouse nurseries per-
manent portrays are fitted on benches and numbers of portrays may very
as per the capacity. These playhouses are controlled with sensors for auto-
mated irrigation system and cooling. Seedling production capacity may
range from 2-10 lakhs per batch.
Prepare Rooting Mix
Rooting media should be soilless media, light and porous which retains
moisture and allows proper drainage and free from weed seeds and any soil
borne pathogens or insects. Different types of media combination for plug
trays: i) Cocopeat: Sand: FYM: vermicompost (2:1:0.5:0.5) or Cocopeat:
Vermiculite: Perlite (3:1:1), ii) Fine soil: Sphagnum Peat Moss: Perlite
(2:1:2), iii) Fine Sand for induction of rooting of stem cuttings, iv) Sand:
Soil: FYM: Rice Husk Ash (1:1:1:1), v) coco peat (70 Kg) and neem cake
(1kg) + Azospirillum and Phosphobacteria (each @ 1 Kg).
Crops
Flowering plants: a) seedlings of marigold, pansy, china Easter, sylvia,
california poppy, celosia, petunia, ornamental chilli/Cabbage and sun flow-
er etc. b) stem cuttings of carnation, coleus, dahlia, chrysanthemum, fuch-
sia, geranium, petunia, impatiens, rose etc., c) suckers of chrysanthemum
and gerbera etc., d) gladiolus corms, dahlia and lilium bulbs.
Vegetables: a) seedlings of transplanted vegetables such as capsicum,
cauliflower, cabbage, broccoli, brussels sprouts, tomato, brinjal and chilli b)
seedlings of directly sown vegetables suck as cucumber, bottlegourds,
pumpkin, ridgegourds and hyacinth bean etc., c) suckers of vegetables such
as spinegourds and pointedgourds Even crops namely ginger and turmeric
micro-rhizomes can be sown in the plug trays.
Crop
Seed Rate per Ha.
Portray Open
Beds
Tomato 100g 500-600g
Brinjal 200g 400-500g
Chilli and Capsicum 200g 300-500g
Cauliflower and Cabbage 250g 400-600g
Marigold 1.25kg 1.5 kg
Different
Types
of
Portrays/Plugtrays
Plug Plants
Tomato
Cauliflower
Fuchsia
Anthurium
Strawberry
Gerbera

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ICAR Guide to Plug Nursery Production of Quality Vegetable and Flower Seedlings

  • 1. Indian Council of Agricultural Research ICAR Research Complex for NEH Region Tripura Centre, P.O. Lembucherra-799210 Acknowledgement: We sincerely thank to Dr. Narendra Prakash, Director, ICAR RC NEH Region for his guidance and to all who contributed directly or indirectly for pre- paring this manuscript. Disclaimer: Some contents and photos are taken from difference sources to make the topic more elaborative for farmer’s easy understanding and for welfare of farmers. Sources/ person are hereby well acknowledged . Prepared & Designed by: Dr. Biswajit Das, Principal Scientist, Horticulture, ICAR RC, Tripura Centre Published by : The Joint Director, ICAR RC for NEH, Tripura Centre For more details please contact: Dr. B. K. Kanpal Joint Director ICAR Research Complex for NEH Region, Tripura Centre, Lembucherra, Tripura (West) - 799210 E-mail : jd.tripura@Icar.gov.in Dr. Biswajit Das Principal Scientist, Horticulture ICAR RC, Tripura Centre E-mail: biswajitsom_dr@yahoo.co.in Copyright ©: ICAR-NEH Region, Tripura Centre. Year 2018 Publication No. 56 Plug Nursery for quality planting material production Plugs for vegetable seedlings Vegetable portray nursery can be raised under normal net house or naturally venti- lated polyhouses. However, advanced Hi-tech polyhouses with capacity of produc- ing 5-10 lakhs seedling per batch may be established depending upon the require- ment. Portrays with grownup seedling are as such transported even to long distance areas for transplanting. Portray nursery of ginger Ginger rhizomes are sprouted by placing on sands bed and when buds are bulged all the rhizomes are taken out of the sand deb. Rhizomes are cut into small pieces each segment having one bulged bud. Each piece is put into portrays and filled with soil- less media such as cocopit + vermicompost. After about 2 months plants are ready for transplanting. Transplant the Plug Plants Carefully Plug plants earth cone is loosened by slight tapping and care is taken that earth and root zone remain moistened, but conical shape of the earth does not break and re- mains intact when taken out from the tray cells for transplanting. Vegetable plugs are transplanted directly into the field by making hole at crop specific spacing. Ornamental/flower plants are planted in pots which are filled with moistened pot mix and a hole is made slightly bigger in size than the plug plant earth cone at the center. Thus, raising flower plants or vegetable crops from plug plants improved the Seed Germination 100% & Uniform Seedling Growth Plug Plants: No transplant shock & plant death Biswajit Das H. Lembisana Devi B. K. Kandpal Points to be noted 1. Rooting media should be light and soil borne diseases-insects free . 2. No water stagnation inside the tray cells. 3. Temperature should be 20-30 0 C for better seed germination. 4. Seedling should be over mature to avoid extra growth of roots. 5. Prior to transplanting hardening and acclimatization is essential. 6. Seedlings may be affected by rotting at the color region due to Damping off disease, if rooting media or seeds are not treated. Drench the media with carbendazim and mancozeb mixture or metalyxil (15-20g/10L water). 7. Appropriate insecticides may be sprayed for insects such as monocrotophos or metasystox (1.5 ml/L water) for leaf minor (white tunnels along the veins in the leaf lamina), thiamethoxame (1ml/L water) for whiteflies and neem oil (2-3ml/L water) or chlorpyrifos/dimethoate/quinalphos (1ml/L water) for any leaf damaging insects. 8. Plugtrays/portrays should be handle with care and reused. 9. More than one seedling may be raised per cell of portrays for ease of trans- portation in case of transplanted vegetable crops and to minimize cost, how- ever, each seedling is separated with care at the time of transplanting. Quality Seedlings Determine Quality of Produce
  • 2. Quality planting material is the basic requirement for production of high value flowers and vegetables. It is not possible for each gardener or farmer to raise nurseries for all the plants they are interested to grow. In the recent years, plug plant nurseries have become very popular in India for high value flower and vegetable crops raised under protected or open conditions. Continue….. What is Plug nursery? Seedlings or nursery plants are grown as a separate unit in modular plugtrays/portrays with separate cells/conical cavities containing growing media and transplanted with intact root soil. Advantages a) Costly seeds of high value crops or hybrids and portrays ensure germina- tion of each seed, b) Growing of such plants namely lilium or tulip etc. whose bulbs can not be produced under Tripura climate, c) Management practices can be employed for better and uniform growth of plant and root, c) Less affected by disease-insects and weeds, d) Long distance transport without any disturbances, e) Transplanted to field or container in any season without any shock, and f) Maximum field survivability. Treat the Rooting Media Solarization by exposing the soil under sun and airtight covering the soil with polyethylene for 5-6weeks. So that temperature inside increases and organism gets killed without oxygen. Drenching the soil mix with formalin 2-5% solution (200-250ml/10 L) upto a depth of 15-20 cm @ 4-5 L/m2 soil surface and immediately covered with black polyethylene sheet for 6-7 days. The mix is raked after removing the polyethylene cover and is left exposed for at least 4-5 days. Soil may be drenched with solution of car- bendazim or captan (3-4g/L) @ 1-2L/m2 soil surface. Phorate powder (5g/ m2 mixture) is also applied in dried soils. Fill the Portrays with Rooting Media Approximately 1.0-2 Kg of the coco peat medium is filled in each portray depending upon the size. Each cells of the tray are filled with the mix upto 1 cm below the rim and slightly pre-moistened by sprinkling water on the trays and top surface of the mix of each cells are leveled by a piece of wood frame or rod. Treat the Seeds Seed treatment is essential as prophylactic measure. Commercially availa- ble seeds are pretreated with fungicides. However, it is beneficial to treat seeds prior to sowing with captan or carbendazim (@2-3g/L water/kg seed). Biofungicides such as Pseudomanas fluorescens (10g/Kg seeds) or Tricho- derma viride (4g/kg seed). Seeds may be mixed with Azospirillum @ 200g/ kg seed and are shade dried for half an hour. Seed Rate Requirement of seed per hectare is less for portray nursery raising system in comparison to open bed nursery raising of vegeta- bles and flowers. Sow the Seeds in Portrays A small hole (1-2 cm deep) is made at the centre of each cell by a thin stick. Seed is put into each hole and covered with fine potting mix by slightly pressing with thumb tip, so that, no air space is left around the seed. The trays may be covered with dry grass/hay straw and is removed just after germination. Soil is kept mois- tened by gently sprinkling water with rose cane. Germination takes place within 7-9 days after sowing. Selective weeding is done and at 3-4 week stage water soluble fertilizer as per requirement is given along with irrigation. At 6-8 week stage plug trays containing seedlings (8-12 cm height, 4-6 leaf stage or even more in case of flowering plants) are ready for transportation and transplanting. Prior to transporta- tion or field planting hardening for 3-5 days is beneficial for better survival. Portrays/plugs for Chrysanthemum Plants are cut above 7-10 cm from ground level after flowering when flow- ering is over. Several suckers arise laterally at the base of the plant. Suckers are separated and planted plug cells 10-15 cm. Soft stem cuttings are taken from healthy stock plants from end of September-October or February to middle of June. Five to ten cm long cuttings are made by shearing the basal leaves. For enhancing rooting these cuttings can be treated with 500 ppm NAA + 100-200 ppm IBA for 5-10 sec. Treated cuttings are planting in plug trays containing soilless media. Plugs for Rose plants ‘Stenting’ is an advanced and quickest method of rose propagation. In this method, buds of suitable cultivars are budded on local unrooted rootstocks and kept in IBA (500-1000 ppm) for 15-20 sec. The budded unrooted root- stocks cuttings are kept for rooting under intermittent mist in plug trays. Semi hard wood cuttings of 6-8 mm diameter and 10-20 cm long are col- lected from actively growing mother plants. Cuttings are inserted 5-10 cm into the rooting media. Rooting and bud union takes place simultaneously. Otherwise, rooted cuttings of Rosa indica, Rdward rose or Rosa multiflora or any other local hardy roses may be raised in plug trays and later on bud- ding is done. Plugs for Gerbera Gerbera may be propagated by seeds, separation of crown clumps or suck- ers and micro-propagated plantlets which are specially raised in plug trays. Gerbera seeds are very minute and lose viability, if sowing is delayed. Each seed is carefully sown in individual cell containing pre-moistened pot mix of peat: perlite (1:1) and covered with a thin layer of vermiculite. Trays are kept in high humidity germination chamber at RH 90-95% and temperature 20-24 0 C. Germination starts after 7-14 days and then all the seedling trays are shifted to polyhouse. Suckers/clumps are separated from the mother plant during June-July and planting into the plug trays. Tissue culture raised plantlets are planted into plug trays containing perlite: sphagnum moss (1:1) in the hardening chamber and after acclimatization plants are transported or planted in the field. Plugs for Anthurium Division of roots, separation of suckers, seeds and micro-propagated plantlets are methods of Anthurium multiplication. Offshoots arising laterally from the base of the main stem of mother stock are separated along with portion of arial roots. Around 14-15 offshoots may be collected from a single plant per year. These off shoots are planted in the plug trays in individual cells containing peat: pine bark/ coconut husk: perlite (2:2:1). Trays are kept in shade house under indirect sunlight and moist and humid condition. Tissue culture raised plantlets are transferred to small plug trays containing vermicompost: sand (1:3) or sand: charcoal (3:1) or pear: perlite: sand (1:1:1) in the hardening chamber for hardening. Requirements The essential requirements are Plugtrays/Portrays of different Nos. and cell dimensions. Cell number per tray may vary from 12 to 512 depending up- on the requirement and plant type and each cell volume may vary from 3 to 300 cc. Normally 98 cells (54 x 27cm) of 3.5 cm diameter and 0.8mm thickness Portrays are made of UV stabilized PVC or biodegradable plas- tics with thickness of 0.85 -1.5 mm. Protected structures Shade house/net house/polytunnels/naturally ventilated poly house with well ventilation and irrigation facility. In Hi-tech polyhouse nurseries per- manent portrays are fitted on benches and numbers of portrays may very as per the capacity. These playhouses are controlled with sensors for auto- mated irrigation system and cooling. Seedling production capacity may range from 2-10 lakhs per batch. Prepare Rooting Mix Rooting media should be soilless media, light and porous which retains moisture and allows proper drainage and free from weed seeds and any soil borne pathogens or insects. Different types of media combination for plug trays: i) Cocopeat: Sand: FYM: vermicompost (2:1:0.5:0.5) or Cocopeat: Vermiculite: Perlite (3:1:1), ii) Fine soil: Sphagnum Peat Moss: Perlite (2:1:2), iii) Fine Sand for induction of rooting of stem cuttings, iv) Sand: Soil: FYM: Rice Husk Ash (1:1:1:1), v) coco peat (70 Kg) and neem cake (1kg) + Azospirillum and Phosphobacteria (each @ 1 Kg). Crops Flowering plants: a) seedlings of marigold, pansy, china Easter, sylvia, california poppy, celosia, petunia, ornamental chilli/Cabbage and sun flow- er etc. b) stem cuttings of carnation, coleus, dahlia, chrysanthemum, fuch- sia, geranium, petunia, impatiens, rose etc., c) suckers of chrysanthemum and gerbera etc., d) gladiolus corms, dahlia and lilium bulbs. Vegetables: a) seedlings of transplanted vegetables such as capsicum, cauliflower, cabbage, broccoli, brussels sprouts, tomato, brinjal and chilli b) seedlings of directly sown vegetables suck as cucumber, bottlegourds, pumpkin, ridgegourds and hyacinth bean etc., c) suckers of vegetables such as spinegourds and pointedgourds Even crops namely ginger and turmeric micro-rhizomes can be sown in the plug trays. Crop Seed Rate per Ha. Portray Open Beds Tomato 100g 500-600g Brinjal 200g 400-500g Chilli and Capsicum 200g 300-500g Cauliflower and Cabbage 250g 400-600g Marigold 1.25kg 1.5 kg Different Types of Portrays/Plugtrays Plug Plants Tomato Cauliflower Fuchsia Anthurium Strawberry Gerbera