Extraction of bioactive compounds by using cinnamomum iners
1. Evaluation of Anti-Malaria Potency of
Various Extracts of Cinnamomum iners
Bark
Norasheila Binti Mohd Saad
152167
BSc. (H)-Industrial Chemistry
2. Introduction: Pusat Penyelidikan
Dadah & Ubat- Ubatan
• First established on recommendation by World Health
Organization (WHO) in 1973
• Selected as one of the Higher Institutions' Centres of
Excellence (HICoE) by the Ministry of Higher Education.
• Established Memorandum of Understanding (MoU) with
Pharmaniaga Berhad
• Provide consultancy services to advise on the
optimization of analytical, extraction process and the
identification of phytocompounds for the Kacip Fatimah
Project.
3. • Vision
• Leading in drug research
• Mission
• To conduct relevant, innovative and quality research
• To disseminating new kind of useful knowledge to
serve mankind
4. Introduction: Cinnamomum iners
• Local name :
• Kayu Manis Hutan
• English name : Wild Cinnamom
• Diagnostic characters :
• Leaves pink when young
• Glaucous beneath with three
longitudinal veins
• Crushed fresh leaves and inner
bark with strong smell of
cinnamon
• Fruit green with yellow spots
when young, dark blue when
ripen
5. Project
• Extraction process by using soxhlet
method
• Used 4 types of solvents; petroleum
ether, dichloromethane, methanol and
water
• Extraction of the tree’s bark
• Trunk straight, bark smooth, greyish-
brown, inner bark pinkish with strong
fragrant smell.
• The timber is insect resistent and used
for house building and cabinet work.
• The bark yields an inferior grade of
cinnamon but oil distilled from it and
from the leaves can be used for
flavouring and for incense sticks.
6. Method
Grinding • The bark was grinded by using grinder
Extraction • Soxhlet method
Analysis
• GC-MS
• IR
• Enzyme assay
• Paper chromatography
8. Extraction
25 g of dried, grinded C.
iners bark was weighed
and transferred into a
thimble
The soxhlet apparatus
was set up; apparatus
consisted of chiller at the
top, soxhlet in the middle
and round bottom flask at
the bottom
A few anti bumping chips
were added into the
round bottom flask
Solvent was added until
the round bottom flask
was half full. ~600ml
Chiller and heat was
turned on and the
temperature was set
Solvent was dried by
using rotary evaporator
Procedure was repeated
by using
dichloromethane, ethanol
and deionized water
23. Experience/ knowledge
• I obtained precious experiences regarding on how to
conduct research like a postgrad student
• Basically, all related more to organic chemistry, sub
category of natural products.
• Lab technicians explained step by step procedures on
how to prepare the samples, analyze them and also how
to interpret the results.
• All the information obtained are hard to find at
somewhere else.
24. Conclusion
• The industrial training at here is very fun and exciting.
• This is because the students are exposed on how to
handle the machine ourselves and at the same time new
precious experiences are obtained.
• The Center provides good facilities and modern
instruments.
• Thus, faster results can be attained.
• This helps to save a lot of time and also give precise
results.
25. Comment/suggestion
• By only using GC-MS, FT-IR, enzyme assay and paper
chromatography, the identities of the compounds are
still not well known.
• Thus, further analysis should be done in order to reveal
more the structure and activity of the compounds.
• This Center is a good place for students to do internship
or postgrad, especially for people that are interested in
organic chemistry and research field.
• The students must be high discipline and able to work
independently.