Connective tissue is formed of cells, fibers and matrix Here; the free cells of the CT

1. 1

2. CONNECTIVE TISSUE
Connective Tissue Cells a- Fixed ,
b- Free
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3. CONNECTIVE TISSUE
Free CT cells
 Plasma cells
 Mast cells
 Leucocytes
 Pigment cells
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4. CONNECTIVE TISSUE
Plasma cells
LM:
Shape : Large spherical cell (20 µm)
Nucleus : spherical and eccentric nucleus
Cytoplasm: Basophilic in H&E stain.
It shows a pale area close to the nucleus with a well developed Golgi
called (Negative Golgi Image), spherical inclusions called (Russell
Bodies) . 4Dr. M.Attia

5. CONNECTIVE TISSUE
Plasma cells
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6. CONNECTIVE TISSUE
EM:
 The nucleus has large clumbs of heterochromatin alternating with
light area of euchromatin giving the clock face appearance.
 The cytoplasm contains mitochondria, rough endoplasmic reticulum,
Golgi complex, many free ribosomes.
 Function:
Immunoglobins production ( Antibodies)
Plasma cells
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7. CONNECTIVE TISSUE
Mast cell
Pluripotent stem cell in the bone marrow
It has specific surface receptors for the Ig E
antibodies produced by plasma cells.
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8. CONNECTIVE TISSUE
Mast cell
LM:
Shape : Large ovoid cell (20 µm)
Nucleus : spherical and central nucleus
Cytoplasm: filled with large basophilic granules in
H&E stain , shows metachromasia.
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9. CONNECTIVE TISSUE
EM:
 The cytoplasm contains mitochondria, rough endoplasmic
reticulum, Golgi complex
 Granules appear as electron dense spherical membranous
bounded.
Mast cell
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10. CONNECTIVE TISSUE
The mast cell granules contain :
A- Primary mediators: heparine, histamine, serotonin, eosinophil,
chemotactic factor.
B- Secondary mediators : substance of anaphylaxix and
prostaglandins.
Mast cell
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11. CONNECTIVE TISSUE
Mast cell
Functions
1- Allergic reaction and inflammation
2- Anticoagulant reaction
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12. CONNECTIVE TISSUE
Pigment cell
Cells containing pigments are found in:
Choroid of the eye
Pia mater
Dense CT of the skin.
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13. Leucocytes (WBCs)
CONNECTIVE TISSUE
They are colorless in fresh state
They are found in the blood stream, CT are pleomorphic
Total leucocytes count is 4000 to 11000 per cubic
millimeter
They are either Granulocytes or A GRANULOCYTES

14. Granulocytes
Neutrophils
Basophils
Eosinophils
A granulocytes
 Lymphocytes
Monocytes