Mycology

1. INSTITUTE OF HEALTH TECHNOLOGY, DHAKA
Department of Laboratory Medicine
BSc in Health Technology (Laboratory)- 1st Year
MYCOLOGY
Lecture No. 7.2 (Opportunistic Mycoses -Rhinosporidiosis)
By
Sk. MIZANUR RAHMAN
Lecturer, Mycology
MS in Biotechnology & Genetic Engineering (UODA)
MS in Microbiology (SU)

2. Rhinosporidiosis
Sites: nasal, oral, (palate, epiglottis), conjunctiva
Lesion: polyps, papilomas, warts-like lesion
More seen in communities near swamps
Etiology: Rhinosporidium seeberi
Laboratory diagnosis:
specimens, biopsy tissue
Direct microscopy: stained section or smears KOH, will
show spherules with endospores
Culture on SDA will be negative

3. • Rhinosporidiosis is an infection of the
mucocutaneous tissue caused by Rhinosporidium
seeberi, an as yet unisolated and unclassified
fungus.
• It causes a chronic granulomatous disease
characterised by the production of large polyps,
tumours, papillomas, or wart-like lesions. The
nose is the most commonly affected site.
Rhinosporidiosis.

4. Rhinosporidiosis.
• 1st identified in Argentina, but majority of cases
occur in India and Sri lanka.
• High incidence among people who frequently
bath along with domestic animals in ponds,
tanks, lakes

5. Clinical Features
• Chronic granulomatous disease of mucous
membrane.
• Characterised by the development of friable
polyps in the nose, mouth or eye.
• Miscellaneous forms –
Buccal cavity,vagina,
vulva, penis, urethra
or rectum

6. Laboratory Diagnosis
• Cannot be cultured
Direct Examination
• FNAC, Biopsy of lesion, Nasal washing
- Contains sporangia
filled with thousands of
sporangiospores(6-9µ)
embedded in a stroma
of connective tissue &
capillaries

7. 431

8. 432

10. Rhinosporidium seeberi
• Title: Rhinosporidiosis
• Disease(s): Rhinosporidiosis
• Legend: Rhinosporidiosis. Sporangia in a human polyp, GMS stain.
• Genus/Species: Rhinosporidium seeberi
• Image Type: Histopathology