Cancer Cell lines are the continuous cell lines that keep on dividing under conditions provided in vitro. Primary neoblastic tissues which are abnormal as they keep on dividing are dissociated by enzymatic or enzymatic in combination with chemical or by mechanical disaggregation means. Depending upon the architecture of the tissue different methodologies are adapted. The presentation focuses on :- 1. Milestones in cell line research 2. How cancer cell lines are established 3. Method for disaggregation 4. Selective Culture of Tissue Cells 5. Specific Tumor Types - focusing on properties like adherent/suspension and different media provided in the cell lines 6. Problems Associated with Cancer Cell Lines 7. Some Applications 8. References

1. CELL LINES: CANCEROUS ORIGIN
Krittika Zutshi (20040342017)
Lavanya Lulla (20040342018)

2. Table of Contents
● Definition of Cancer Cell Lines
● Milestones in Cancer Cell Culture
● Introduction
● Disaggregation Techniques
● Selective culture of Cancer Cells
● Specific Tumor Types and its medium
● Problems Associated with Cancer Cell Lines
● Applications
● References

3. WHAT IS A CANCER CELL LINE?
● Cancer Cells that keep on
dividing and growing over
time.
● In Vitro model System.
● Widely used in medical
research and drug discovery.

4. MILESTONES IN CANCER CELL
CULTURE
Immunofluorescence image
of HeLa cells
George Gey and
Leonard Hayflick

5. INTRODUCTION
Source: Mitra, A., Mishra, L., & Li, S. (2013). Technologies for deriving primary tumor cells for
use in personalized cancer therapy. Trends in biotechnology, 31(6), 347-354.

6. DISAGGREGATION

7. SELECTIVE
MEDIA
HISTOTYPIC
CULTURE
SUSPENSION
CLONING
CONFLUENT
FEEDER
LAYER
SELECTIVE
CULTURES OF
TUMOR
CELLS
● HITES medium
● Low serum/
serum-free medium
● Antibiotics
● Pre-formed confluent
monolayer of normal
cells.
● For example:
epithelial cells for
adenocarcinoma
cells. And glial cells
for glioma tumors.
● For highly clonogenic
cells.
● For tumor biopsies and
drug screening.
● Spheroid culture
● Filter well inserts

8. SPECIFIC TUMOR
TYPES

11. MEDIA FOR CONTINUOUS CELL
CULTURES

13. APPLICATIONS
● Screening of anti-cancer
drug
● Cell based Bioassay
● Determination of
cytotoxicity
● Production of antiviral
Vaccines
● Study of toxic and pollutants
using cell lines
● Chromosome analysis

14. REFERENCES
1. Handbook: Cancer cell culture basics
2. Gillet, J. P., Varma, S., & Gottesman, M. M. (2013). The
clinical relevance of cancer cell lines. Journal of the National
Cancer Institute, 105(7), 452-458.
3. Wilding, J. L., & Bodmer, W. F. (2014). Cancer cell lines for
drug discovery and development. Cancer research, 74(9),
2377-2384.
4. Freshney, R. I. (2015). Culture of animal cells: a manual of
basic technique and specialized applications. John Wiley &
Sons.
5. Langdon, Simon P. Cancer cell culture. Vol. 5. No. 9. Humana,
2010.