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Microscopes
Development Over Time The first microscope was made in England at the end of the 16 th  century and was believed to the result of looking through the wrong end of a telescope. In 1597 Zaccharias Jansseen and his son, Han Jansseen, realised that two lenses, arranged one at the eyepiece and one near the sample, resulted in a significant enlargement. As the designs were still based around telescopes they were three feet tall and heavy and were also ineffective with a magnification level of 9x.  Antony Van Leeuwenhoek was the first man to make and use a useful microscope with a magnification of 270x in the late 17 th  century. This was the result of superior lenses he produced by grinding and polishing a small glass ball. Microscopes used today generally refer to compound microscopes that use multiple lenses to in order to reduce the focal length without making the image difficult to distinguish. Until the mid 18 th  century microscopes were limited by lens quality. Research by Otto Schott on optical glass along with the production of fine optical tools addressed this limitation. And through Ernst Abbe's study of optical principles resulted in a maximum resolution 10x greater than Leeuwenhoek's microscope was achieved in 1870.  During the 18 th  and 19 th  centuries the microscope evolved to be much like how we see them today. It included the addition of stages and slides, as well as the practise of staining samples to make the images much clearer. The 20 th  century had few changes to the basics of light microscopes, but for a standardisation of parts. However, with the invention of the electron microscope allowed samples to be viewed three dimensionally and at incredible magnification levels of 200,000x. ,[object Object]
Light Microscope Preparing a specimen: Biological specimens must also be fixed in order to preserve their structure. This can be through drying or freezing but is usually done chemically. Larger specimens have to be sectioned before they are mounted. A microtome can automatically cut thin slices. If the specimen is frozen a cryostat is used to keep it frozen during the slicing process. Specimens may be transparent and may require staining. Methylene blue is used for animal cells while a potassium iodide solution is used for plant cells. Finally it is mounted on a microscope slide and covered with a cover slip. With consideration to avoiding dust and thumb prints. How it works: A source of light is directed through use of a mirror to shine through a thin specimen mounted on a transparent slide. An objective lens focuses the object within the microscopes tube. The images is magnified by a second lens which is what is looked through, the ocular lens. ,[object Object]
http://nobelprize.org/educational/physics/microscopes/phase/preparation.html

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