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iGrow | A Service of SDSU Extension
| livestock
livestock
Introduction
Distillers grains (DG) originating
from the corn-based fuel ethanol
industry are an excellent source of
energy and non-rumen degradable
protein, and are an effective
ingredient for dairy cattle diets.
However, in spite of competitive
price when compared to other
protein sources and their high
availability in the market, DG are
not always sought as a dietary
ingredient by some nutritionists
and dairy producers. In a recent
survey conducted in South Dakota
by South Dakota State University
Dairy Extension, where 28%
of all Grade A dairy producers
replied (Unpublished. 2011), many
confirmed they did not use DG in
their dairy cattle diets.
Survey of attitudes
In another survey on the use
of DG, sent to 10 nutritionists
specializing in dairy cattle (0wens,
F. 2009), their high fat content
was the main reason why DG
inclusion was restricted in dairy
diets. Half of those surveyed (5
out of 10), agreed that the high
concentrations of unsaturated
fatty acids (FA) in DG reduced fat
content in milk. Nine out of ten of
Composition of Fat in Distillers Grains
Fernando Díaz-Royón | DVM Álvaro García | Dairy Specialist, DVM, Ph.D.
Kurt A. Rosentrater | Adjunct Assistant Professor, South Dakota State Univeristy, Agricultural  Biosystems Engineering, Iowa State University
dairy sciencefebruary 2012
the dairy consultants indicated that
the level of DG inclusion in diets
could be increased if a portion
of the fat in DG was removed.
In addition, these nutritionists
agreed that the cost of DG as a
feed ingredient should be reduced
proportionally to the energy
content reduction as a result of fat
extraction. Estimations as to what
degree the cost of DG should be
reduced varied from 2% to 50%,
with an average of 24%.
In another survey (Unpublished,
2011), individual South Dakota
dairy producers were asked if
they used DG in their diets, and
to rank from 1 to 4 the degree
of importance of fat content
in DG (1 = no importance; 2 =
low importance; 3 = average
importance; and 4 = high
importance). Results indicated an
average importance of 3.3. This
parallels another survey, published
by the National Agricultural
Statistics Service (NASS, 2007),
which showed that variation in
DG fat content was a concern to
dairy producers. On a scale of 1 to
4 (similar to the aforementioned
study), the average was slightly
higher at 3.6. Thus, results
obtained from all these surveys
provide considerable evidence
that the high fat content in DG,
together with high concentrations
of unsaturated fatty acids, can
be a matter of worry for dairy
consultants and producers alike.
Fat in distillers grains
Several publications have reported
the nutrient composition of a
large number of distillers dried
grains with solubles (DDGS)
samples, and have indicated fat
content values between 10.9%
and 12.6% on a dry basis (DM;
Table 1). Coefficients of variation
(CV) for published fat content
values are typically not extremely
high within a given study, with
published values of approximately
6.6% (Belyea et al. 2004) and
7.8% (Spiehs et al. 2002). Results
published by the New York State
Dairy One Lab (4819 samples
in 2011), however, showed an
average fat content in DDGS of
12.6% (DM), but with a range of
values from 9.4 to 15.7%, and
a CV above 25%. These results
indicate very high variability in
DDGDS fat content.
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| livestock
The nutrient composition of corn
DDGS, published in different
books and nutrition guides, also
show high variability. The NRC
(2001) reports a fat content of
10%, on a dry basis, which is
similar to nutrient composition
tables of the Spanish Foundation
for the Development of Animal
Nutrition (FEDNA) at 10.9%. The
nutrient composition of feedstuffs
tables of the Institut National
de la Recherche Agronomique
(INRA, 2004), however, published
a value of 4.4%. This wide range
in published data suggests that
the information in books about
the fat content in DDGS cannot
be completely trusted, so it is
suggested to formulate diets
based on actual chemical analyses
of ingredients, rather than using
tabular values.
One of the major causes of
DDGS fat variation is the amount
of condensed distillers solubles
(CDS) added back to the non-
fermented wet cake prior to
drying (which then produces
DDGS). The amount of fat in the
condensed solubles is greater
than in the wet cake, and can
represent almost a 21.5% of the
DM in the CDS (Schingoethe et
al. 2010). Ganesan et al. (2005)
evaluated the effect of the fat
content in DDGS as the proportion
of CDS inclusion increased. The
fat level in DDGS increased from
8.8% up to 11.8% of the DM
when the CDS increased from 10
to 25%. Noll et al. (2007) also
documented an increase in DDGS
fat content with an increase of
CDS addition.
Fat composition
The fat in DDGS is mainly
composed of unsaturated FA;
this profile is reflected in six
research trials summarized in
Table 2. Linoleic (C18:2) and oleic
(C18:1), are the most abundant FA,
with an average of almost 50%
(CV=14.2%) and 25% (CV=28.9%)
of the total FA, respectively.
The high CV observed could be
the result of the analyses being
performed by different labs, and
also maybe because of variations
in extraction methods and
analyses. The average values for
the most abundant FA are similar
to those reported by Moreau et al.
(2011). These authors found that
the linoleic and oleic content of
DDGS from three different ethanol
plants were 53.7 and 25.6%,
respectively; the CV, however,
were substantially lower at 1.7%
and 1.3% for linoleic and oleic,
respectively. In addition, this
trial determined the variations in
the FA profile during the ethanol
production process by analyzing
corn grain, DDGS and seven
intermediate products (Table
3). Although with some slight
differences in the exact values
between different fractions, the
FA profile varied little during the
production process, with the
exception of the predominant FA,
which generally remained constant.
Bauman and Griinari (1998)
demonstrated that there are two
conditions which can reduce milk
fat. One of them is the presence
of unsaturated FA in the rumen;
the other is an altered rumen
environment that would cause
incomplete bio-hydrogenation.
Under certain conditions,
the pathways for rumen bio-
hydrogenation are altered and
through alternative routes,
intermediaries are produced,
some of which, like trans-10-cis-12
CLA, are potent inhibitors of milk
fat synthesis in the mammary
gland (Griinari et al. 2001). The
concentration of the unsaturated
FA in the rumen can be a key
factor that contributes to changes
in microbial population and an
increase in the CLA isomer trans-
10-cis-12 (Jenkins et al. 2009).
In addition to the degree of
FA unsaturation, the rumen
concentration of free FA (FFA)
should also be considered. This fat
fraction may have greater capacity
to produce negative effects
in rumen fermentation than
other fractions, as for example,
saturated FFA or triglycerides (TG;
Jenkins et al. 1993). Chalupa et al.
(1984) evaluated the production
of volatile FA in vitro in terms of
long chain FA (palmitic, stearic,
and oleic) in the form of FFA or
TG. The FA supplied as TG did not
produce any significant changes
in rumen fermentation. However,
when the FA were supplied as
FFA, there was an increased
production of propionic acid, and
a reduction in the production of
acetic, butyric, and total VFA.
The authors concluded that the
FFA affected fermentation more
than the TG, and that their anti-
microbial activity increased with
their degree of unsaturation.
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The FFA content in corn and
various co-products from seven
ethanol plants are in Table 4.
(Moreau et al. 2011). The FFA
content in the oil extracted from
corn grain was 2.28% (as-is
basis). In DDGS, however, the
average FFA content increased
to 9.1% (as-is basis). In addition,
DDG (distillers dried grains)
and thin stillage, both fractions
obtained after centrifugation, also
contain high concentrations of
FFA (7.4 and 9.4%, respectively).
These results agree with those
published by Noureddini et al.
(2009; Table 5), with FFA values
close to 7.5% for DDGS, whole
stillage, and condensed solubles.
These authors separated FFA into
saturated (palmitic and stearic)
and unsaturated (oleic, linoleic
and linolenic) components. The
unsaturated FFA represented
between 75 and 80% of the total
FFA, while the TG were 91% of
the total fat content of the co-
products (Table 5). Vegetable
oils are, in general, low in FFA
(typically ~ 0.5 – 1.5%). The TG are
protected by protein membranes
in whole seeds with very little
lipolytic activity until germination
(Quettier and Eastmond. 2009).
The reason why it increases in
DG is unknown at the moment,
but possible reasons could be
lipase activity in corn or yeasts,
continuous pH changes, and the
high temperatures used during
evaporation and drying (Winkler-
Moser, 2011).
In recent years there has been
growing interest by many ethanol
plants to extract a portion of
the oil from DDGS or other
process streams as a means
of increasing plant profitability.
Several technologies are available
to recover the oil either before or
after fermentation. One method
consists of fractionating corn into
three different fractions before
fermentation: the endosperm
(starch-rich), germ (oil rich), and
bran (fiber rich). The bran is used
as a high fiber feedstuff, mainly for
ruminants. The germ, which has a
high fat content, can be de-oiled
(and the oil used for human foods)
and the resultant co-product is
called germ meal. This process
allows for only the endosperm,
with a high starch concentration,
to be fermented into ethanol. The
DG obtained by this process will
have high protein content (35-
55%), but lower concentrations of
fat and fiber (Table 6); these DG
are generally labeled high protein
distillers grains or HPDDG.
In recent years there has been
increased interest in extracting the
oil from different fractions after the
fermentation process, but before
drying. Different commercial
processes have been developed
to extract the oil from thin stillage,
semi-concentrated stillage and
even condensed solubles. The
majority of the methods are based
on physical separation techniques,
using different separation columns
or centrifuges, and they are
capable of extracting between
30% and 70% of the oil contained
in the co-product (Rosentrater
et al. 2011). Another possibility
is the chemical extraction with
solvents of the oil in DG. Some
examples of commercial DDGS
obtained through these post-
fermentation processes appear
in Table 7. When part of the oil is
removed, the rest of the nutrients
increase proportionately. The final
fat content is variable, depending
on the method used by each
company, and it ranges between
2.5 and 7.5% (DM); these DG are,
in general, called low fat DG. Due
to their greater FFA content, the
oil obtained after fermentation is
presently used as animal feed or a
substrate for biodiesel production.
The high concentration of
unsaturated FA in DG, together
with high FFA content, can
sometimes lead to milk fat
depression in dairy cattle fed
diets that include high levels of
coproducts. In a meta-analysis of
24 experiments, Kalscheur (2005)
found that use of DG caused milk
fat depression only when diets
had less than 50% forage or less
than 22% NDF from forage. Thus,
new ethanol co-products which
have lower fat content will have
less capacity to modify the rumen
environment and reduce milk fat
concentration and yield.
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Table 1. Typical DDGS composition (% dry matter basis)
Spiehs et al. (2002) Belyea et al. (2004) UMN (2009) Dairy One Lab (2011)
Number of Samples 118 235 49 *
DM, as-fed basis 88.9 NA 89.22 88.1
CP, % 30.2 31.3 30.8 31.17
ADF, % 16.2 17.2 13.7 16.8
NDF, % 42.1 NA NA 33.9
Ash, % 5.8 4.6 5.69 5.87
EE, % 10.9 11.9 11.2 12.57
DM = dry matter; CP = crude protein; ADF = acid detergent fiber; NDF = neutral detergent fiber; EE = ether extract; * Number of
sample ranged by nutrient, from 4,819 to 6,702.
Table 2. Fatty acid composition in DDGS g/100 g of total fatty acids)
FA
Ranathunga
et al. (2010)
Anderson
et al. (2006)
Nyoka
(2010)
Tang et al.
(2011)
Martinez Amezcua
et al. (2007)
Owens T. M.
(2009)
Mean
C12:0 NA 0.78 NA 0.02 0.04 0.01 0.21
C14:0 0.42 2.45 3.95 0.07 0.09 0.38 1.23
C16:0 14.7 15.5 16.9 16.7 12.8 12.5 14.9
C16:1 0.13 NA 2.46 0.16 0.18 0.11 0.61
C18:0 1.99 2.38 2.82 2.62 2.03 1.68 2.25
C18:1 26.9 17 21.4 23.1 23.2 38.2 25.0
C18:2 50.7 52.5 40.2 53.7 56.3 40.3 49.0
C18:3 1.6 4.79 1.44 0.45 1.48 1.05 1.80
C20:0 0.39 1.45 0.55 1.99 0.39 0.26 0.84
C20:1 0.22 NA 3.46 0.29 0.27 0.14 0.88
C20:2 0.03 NA 0.13 NA 0.05 0.03 0.06
FA = fatty acid; NA = not determined
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Table 3. Fatty acid composition of 9 fractions during the ethanol fermentation process manufacturing (% dry
weight basis)
Palmitic
(C16:0)
Stearic
(C18:0)
Oleic
(C18:1)
Linoleic
(C18:2)
Linolenic
(C18:3)
Ground corn 13.3 ± 1.0 1.8 ± 4.0 27.2 ± 1.6 56.5 ± 0.9 1.3 ± 0.8
Cooked slurry 16.2 ± 5.4 2.0 ± 4.1 25.9 ± 2.7 54.3 ± 3.1 1.4 ± 5.0
Liquefied mass 16.4 ± 3.6 1.9 ± 9.3 25.9 ± 1.3 54.4 ± 3.1 1.3 ± 7.9
Fermented mass 15.9 ± 4.0 2.2 ± 5.8 25.9 ± 1.3 54.8 ± 2.2 1.3 ± 3.5
Whole stillage 15.8 ± 4.0 2.2 ± 5.4 26.0 ± 2.0 54.6 ± 2.0 1.3 ± 1.6
Thin stillage 15.3 ± 6.2 2.3 ± 6.5 27.1 ± 1.1 54.2 ± 1.9 1.1 ± 3.3
Distillers soluble 15.8 ± 6.5 2.3 ± 4.5 26.7 ± 2.1 54.0 ± 1.4 1.2 ± 4.7
Distillers grains 16.5 ± 2.8 2.2 ± 3.7 25.3 ± 2.2 54.7 ± 1.5 1.4 ± 1.2
DDGS 16.2 ± 3.4 2.3 ± 2.7 25.6 ± 1.3 54.5 ± 1.7 1.4 ± 1.8
Average ± CV ; Adapted from Moreau et al. 2011.
Table 4. Free fatty acid content in corn and corn co-products (% fresh weight basis)
Corn DDG DDGS TS CDS
Plants (N) 1 5 7 2 1
Average 2.28 7.41 9.13 9.36 10.1
SD 0.02 0.78 1.49 0.63 NA
DDG = distillers dried grains; DDGS = distillers dried grains with solubles;TS = thin stillage; CDS = corn distillers solubles; NA = not available.
Adapted from Moreau et al. (2011).
Table 5. Fat composition from different corn co-products (% fresh weight basis).
SFFA UFFA TFFA G1 G2 G3
DG 1.8±0.2 5.6±0.4 7.4 0.7±0.2 0.6±0.2 91.1±0.3
WS 1.7±0.1 5.9 ±0.3 7.6 0.6±0.1 0.6±0.3 91.3±0.2
CDS 1.5±0.3 5.9±-0.4 7.4 0.5±0.2 0.4±0.4 91.1±0.5
Average ± SD. FFA = saturated free fatty acids; UFFA = unsaturated free fatty acids;TFFA = total free fatty acids; G1 = monoglycerides; G2 =
diglycerides; G3 = triglycerides; WS = whole stillage; CDS = corn distillers solubles. Adapted from Noureddini et al. (2009).
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Table 7. Nutrient composition of low fat distillers grains (LFDDG) (% dry basis)
Solaris Verasun
Dakota
Ethanol
FWS POET 6% POET 8%
DM, as-fed 90.0 87.2 88.1 NA 90.2 90.36
CP 30.0 34.7 30.1 35-37 31.2 31.34
ADF NA 25.3 NA NA 10.5 10.04
NDF NA 31.6 29.0 21 27.6 29.29
Ash 2.50 5.26 4.66 3.8 5.71 5.70
EE 2.50 3.85 7.55 6.50 6.16 8.17
Ca NA 0.10 0.03 NA 0.07 0.08
P NA 0.83 0.92 NA 1.09 1.07
DM = dry matter; CP = crude protein; ADF = acid detergent fiber; NDF = neutral detergent fiber; EE = ether extract; Ca = calcium;
P = phosphorus; NA = not available; POET = compilation of values from Lhrormann. 2006; VERASUN= compilation of values from
Jacela. 2011, Mjoun et al. 2010 a,c: DAKOTA ETHANOL= Personal communication (unpublished); FWS=FWS (2011); POET= Personal
communications (unpublished).
Table 6. Nutrient composition of high protein distillers grains (HPDDG) (% dry basis)
POET Solaris MorTechnology Renessen
Brand Name Dakota Gold HPDDG Glutenol
DM, as-fed 91.7 90 94.09 NA
CP 43.5 47.75 53.39 NA
ADF 10.9 6.7 27.3 35-50
NDF 26.1 34.1 34 7.0-11.0
Ash 2.5 4.65 NA 15-25
EE 3.8 3.55 3.48 NA
Ca 0.02 0.07 0.16 4
P 0.91 0.67 0.32 NA
DM = dry matter; CP = crude protein; ADF = acid detergent fiber; NDF = neutral detergent fiber; EE = ether extract; Ca = calcium;
P = phosphorus; NA = not available; POET = compilation of values from Depenbusch et al. 2008; Robinson et al. 2008; Widmer
et al. 2008; Tedeschi et al. 2009; Abdelqader et al. 2009; Mjoun et al. 2010b; Christen et al 2010; Kelzer et al. 2010. SOLARIS =
compilation of values from: Lohrmann 2006; MOR TECH.= Applegate et al. 2009; RENESSEN = Stern. 2007.
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Tang, S. C., I. Zulkifli, M. Ebrahimi, A.R. Alimon, A.F. Soleimani, K. Filer. 2011. Effects of Feeding Different Levels of Corn Dried
Distillers Grains with Solubles on Growth Performance, Carcass Yield and Meat Fatty Acid Composition in Broiler Chickens. Int. J.
Anim. Vet. Adv., 3(3): 205-211.
Page 9
iGrow | A Service of SDSU Extension
| livestock
Publication: 02-2003-2012
South Dakota State University, South Dakota counties, and U.S. Department of Agriculture cooperating. South Dakota
State University is an Affirmative Action/Equal Opportunity Employer and offers all benefits, services, education, and
employment opportunities without regard for race, color, creed, religion, national origin, ancestry, citizenship, age,
gender, sexual orientation, disability, or Vietnam Era veteran status.
Tedeschi, L. O., P. J. Kononoff, K. Karges, and M. L. Gibson. 2009. Effects of chemical composition variation on the dynamics of
ruminal fermentation and biological value of corn milling (co) products. J. Dairy Sci., 92:401–413.
UMN. 2009. Distillers Grains By-products in Livestock and Poultry Feeds. University of Minnesota. U.S. average compared to DDGS
produced in Canada, China, Spain. Disponible online: http://www.ddgs.umn.edu/profiles.htm
Widmer, M.R., L.M. McGinnis, D.M. Wulf, and H.H. Stein. 2008. “Effects of Feeding Distillers Dried Grains with Solubles, High
Protein Distillers Dried Grains, and Corn
Germ to Growing-Finishing Pigs on Pig Performance, Carcass Quality, and the Palatability of Pork.” J. Anim. Sci. 1910. doi: 10.2527/
jas.2007-0594.
Winkler-Moser, J. K. 2011. Lipids in DDGS. Capitulo 9. Distillers Grains: Production, Properties, and Utilization. Editado por KeShun
Liu and Kurt A. Rosentrater. CRC Press, Florida. Pg. 73 – 102.

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Composition of Fat in Distillers Grains

  • 1. Page 1 iGrow | A Service of SDSU Extension | livestock livestock Introduction Distillers grains (DG) originating from the corn-based fuel ethanol industry are an excellent source of energy and non-rumen degradable protein, and are an effective ingredient for dairy cattle diets. However, in spite of competitive price when compared to other protein sources and their high availability in the market, DG are not always sought as a dietary ingredient by some nutritionists and dairy producers. In a recent survey conducted in South Dakota by South Dakota State University Dairy Extension, where 28% of all Grade A dairy producers replied (Unpublished. 2011), many confirmed they did not use DG in their dairy cattle diets. Survey of attitudes In another survey on the use of DG, sent to 10 nutritionists specializing in dairy cattle (0wens, F. 2009), their high fat content was the main reason why DG inclusion was restricted in dairy diets. Half of those surveyed (5 out of 10), agreed that the high concentrations of unsaturated fatty acids (FA) in DG reduced fat content in milk. Nine out of ten of Composition of Fat in Distillers Grains Fernando Díaz-Royón | DVM Álvaro García | Dairy Specialist, DVM, Ph.D. Kurt A. Rosentrater | Adjunct Assistant Professor, South Dakota State Univeristy, Agricultural Biosystems Engineering, Iowa State University dairy sciencefebruary 2012 the dairy consultants indicated that the level of DG inclusion in diets could be increased if a portion of the fat in DG was removed. In addition, these nutritionists agreed that the cost of DG as a feed ingredient should be reduced proportionally to the energy content reduction as a result of fat extraction. Estimations as to what degree the cost of DG should be reduced varied from 2% to 50%, with an average of 24%. In another survey (Unpublished, 2011), individual South Dakota dairy producers were asked if they used DG in their diets, and to rank from 1 to 4 the degree of importance of fat content in DG (1 = no importance; 2 = low importance; 3 = average importance; and 4 = high importance). Results indicated an average importance of 3.3. This parallels another survey, published by the National Agricultural Statistics Service (NASS, 2007), which showed that variation in DG fat content was a concern to dairy producers. On a scale of 1 to 4 (similar to the aforementioned study), the average was slightly higher at 3.6. Thus, results obtained from all these surveys provide considerable evidence that the high fat content in DG, together with high concentrations of unsaturated fatty acids, can be a matter of worry for dairy consultants and producers alike. Fat in distillers grains Several publications have reported the nutrient composition of a large number of distillers dried grains with solubles (DDGS) samples, and have indicated fat content values between 10.9% and 12.6% on a dry basis (DM; Table 1). Coefficients of variation (CV) for published fat content values are typically not extremely high within a given study, with published values of approximately 6.6% (Belyea et al. 2004) and 7.8% (Spiehs et al. 2002). Results published by the New York State Dairy One Lab (4819 samples in 2011), however, showed an average fat content in DDGS of 12.6% (DM), but with a range of values from 9.4 to 15.7%, and a CV above 25%. These results indicate very high variability in DDGDS fat content.
  • 2. Page 2 iGrow | A Service of SDSU Extension | livestock The nutrient composition of corn DDGS, published in different books and nutrition guides, also show high variability. The NRC (2001) reports a fat content of 10%, on a dry basis, which is similar to nutrient composition tables of the Spanish Foundation for the Development of Animal Nutrition (FEDNA) at 10.9%. The nutrient composition of feedstuffs tables of the Institut National de la Recherche Agronomique (INRA, 2004), however, published a value of 4.4%. This wide range in published data suggests that the information in books about the fat content in DDGS cannot be completely trusted, so it is suggested to formulate diets based on actual chemical analyses of ingredients, rather than using tabular values. One of the major causes of DDGS fat variation is the amount of condensed distillers solubles (CDS) added back to the non- fermented wet cake prior to drying (which then produces DDGS). The amount of fat in the condensed solubles is greater than in the wet cake, and can represent almost a 21.5% of the DM in the CDS (Schingoethe et al. 2010). Ganesan et al. (2005) evaluated the effect of the fat content in DDGS as the proportion of CDS inclusion increased. The fat level in DDGS increased from 8.8% up to 11.8% of the DM when the CDS increased from 10 to 25%. Noll et al. (2007) also documented an increase in DDGS fat content with an increase of CDS addition. Fat composition The fat in DDGS is mainly composed of unsaturated FA; this profile is reflected in six research trials summarized in Table 2. Linoleic (C18:2) and oleic (C18:1), are the most abundant FA, with an average of almost 50% (CV=14.2%) and 25% (CV=28.9%) of the total FA, respectively. The high CV observed could be the result of the analyses being performed by different labs, and also maybe because of variations in extraction methods and analyses. The average values for the most abundant FA are similar to those reported by Moreau et al. (2011). These authors found that the linoleic and oleic content of DDGS from three different ethanol plants were 53.7 and 25.6%, respectively; the CV, however, were substantially lower at 1.7% and 1.3% for linoleic and oleic, respectively. In addition, this trial determined the variations in the FA profile during the ethanol production process by analyzing corn grain, DDGS and seven intermediate products (Table 3). Although with some slight differences in the exact values between different fractions, the FA profile varied little during the production process, with the exception of the predominant FA, which generally remained constant. Bauman and Griinari (1998) demonstrated that there are two conditions which can reduce milk fat. One of them is the presence of unsaturated FA in the rumen; the other is an altered rumen environment that would cause incomplete bio-hydrogenation. Under certain conditions, the pathways for rumen bio- hydrogenation are altered and through alternative routes, intermediaries are produced, some of which, like trans-10-cis-12 CLA, are potent inhibitors of milk fat synthesis in the mammary gland (Griinari et al. 2001). The concentration of the unsaturated FA in the rumen can be a key factor that contributes to changes in microbial population and an increase in the CLA isomer trans- 10-cis-12 (Jenkins et al. 2009). In addition to the degree of FA unsaturation, the rumen concentration of free FA (FFA) should also be considered. This fat fraction may have greater capacity to produce negative effects in rumen fermentation than other fractions, as for example, saturated FFA or triglycerides (TG; Jenkins et al. 1993). Chalupa et al. (1984) evaluated the production of volatile FA in vitro in terms of long chain FA (palmitic, stearic, and oleic) in the form of FFA or TG. The FA supplied as TG did not produce any significant changes in rumen fermentation. However, when the FA were supplied as FFA, there was an increased production of propionic acid, and a reduction in the production of acetic, butyric, and total VFA. The authors concluded that the FFA affected fermentation more than the TG, and that their anti- microbial activity increased with their degree of unsaturation.
  • 3. Page 3 iGrow | A Service of SDSU Extension | livestock The FFA content in corn and various co-products from seven ethanol plants are in Table 4. (Moreau et al. 2011). The FFA content in the oil extracted from corn grain was 2.28% (as-is basis). In DDGS, however, the average FFA content increased to 9.1% (as-is basis). In addition, DDG (distillers dried grains) and thin stillage, both fractions obtained after centrifugation, also contain high concentrations of FFA (7.4 and 9.4%, respectively). These results agree with those published by Noureddini et al. (2009; Table 5), with FFA values close to 7.5% for DDGS, whole stillage, and condensed solubles. These authors separated FFA into saturated (palmitic and stearic) and unsaturated (oleic, linoleic and linolenic) components. The unsaturated FFA represented between 75 and 80% of the total FFA, while the TG were 91% of the total fat content of the co- products (Table 5). Vegetable oils are, in general, low in FFA (typically ~ 0.5 – 1.5%). The TG are protected by protein membranes in whole seeds with very little lipolytic activity until germination (Quettier and Eastmond. 2009). The reason why it increases in DG is unknown at the moment, but possible reasons could be lipase activity in corn or yeasts, continuous pH changes, and the high temperatures used during evaporation and drying (Winkler- Moser, 2011). In recent years there has been growing interest by many ethanol plants to extract a portion of the oil from DDGS or other process streams as a means of increasing plant profitability. Several technologies are available to recover the oil either before or after fermentation. One method consists of fractionating corn into three different fractions before fermentation: the endosperm (starch-rich), germ (oil rich), and bran (fiber rich). The bran is used as a high fiber feedstuff, mainly for ruminants. The germ, which has a high fat content, can be de-oiled (and the oil used for human foods) and the resultant co-product is called germ meal. This process allows for only the endosperm, with a high starch concentration, to be fermented into ethanol. The DG obtained by this process will have high protein content (35- 55%), but lower concentrations of fat and fiber (Table 6); these DG are generally labeled high protein distillers grains or HPDDG. In recent years there has been increased interest in extracting the oil from different fractions after the fermentation process, but before drying. Different commercial processes have been developed to extract the oil from thin stillage, semi-concentrated stillage and even condensed solubles. The majority of the methods are based on physical separation techniques, using different separation columns or centrifuges, and they are capable of extracting between 30% and 70% of the oil contained in the co-product (Rosentrater et al. 2011). Another possibility is the chemical extraction with solvents of the oil in DG. Some examples of commercial DDGS obtained through these post- fermentation processes appear in Table 7. When part of the oil is removed, the rest of the nutrients increase proportionately. The final fat content is variable, depending on the method used by each company, and it ranges between 2.5 and 7.5% (DM); these DG are, in general, called low fat DG. Due to their greater FFA content, the oil obtained after fermentation is presently used as animal feed or a substrate for biodiesel production. The high concentration of unsaturated FA in DG, together with high FFA content, can sometimes lead to milk fat depression in dairy cattle fed diets that include high levels of coproducts. In a meta-analysis of 24 experiments, Kalscheur (2005) found that use of DG caused milk fat depression only when diets had less than 50% forage or less than 22% NDF from forage. Thus, new ethanol co-products which have lower fat content will have less capacity to modify the rumen environment and reduce milk fat concentration and yield.
  • 4. Page 4 iGrow | A Service of SDSU Extension | livestock Table 1. Typical DDGS composition (% dry matter basis) Spiehs et al. (2002) Belyea et al. (2004) UMN (2009) Dairy One Lab (2011) Number of Samples 118 235 49 * DM, as-fed basis 88.9 NA 89.22 88.1 CP, % 30.2 31.3 30.8 31.17 ADF, % 16.2 17.2 13.7 16.8 NDF, % 42.1 NA NA 33.9 Ash, % 5.8 4.6 5.69 5.87 EE, % 10.9 11.9 11.2 12.57 DM = dry matter; CP = crude protein; ADF = acid detergent fiber; NDF = neutral detergent fiber; EE = ether extract; * Number of sample ranged by nutrient, from 4,819 to 6,702. Table 2. Fatty acid composition in DDGS g/100 g of total fatty acids) FA Ranathunga et al. (2010) Anderson et al. (2006) Nyoka (2010) Tang et al. (2011) Martinez Amezcua et al. (2007) Owens T. M. (2009) Mean C12:0 NA 0.78 NA 0.02 0.04 0.01 0.21 C14:0 0.42 2.45 3.95 0.07 0.09 0.38 1.23 C16:0 14.7 15.5 16.9 16.7 12.8 12.5 14.9 C16:1 0.13 NA 2.46 0.16 0.18 0.11 0.61 C18:0 1.99 2.38 2.82 2.62 2.03 1.68 2.25 C18:1 26.9 17 21.4 23.1 23.2 38.2 25.0 C18:2 50.7 52.5 40.2 53.7 56.3 40.3 49.0 C18:3 1.6 4.79 1.44 0.45 1.48 1.05 1.80 C20:0 0.39 1.45 0.55 1.99 0.39 0.26 0.84 C20:1 0.22 NA 3.46 0.29 0.27 0.14 0.88 C20:2 0.03 NA 0.13 NA 0.05 0.03 0.06 FA = fatty acid; NA = not determined
  • 5. Page 5 iGrow | A Service of SDSU Extension | livestock Table 3. Fatty acid composition of 9 fractions during the ethanol fermentation process manufacturing (% dry weight basis) Palmitic (C16:0) Stearic (C18:0) Oleic (C18:1) Linoleic (C18:2) Linolenic (C18:3) Ground corn 13.3 ± 1.0 1.8 ± 4.0 27.2 ± 1.6 56.5 ± 0.9 1.3 ± 0.8 Cooked slurry 16.2 ± 5.4 2.0 ± 4.1 25.9 ± 2.7 54.3 ± 3.1 1.4 ± 5.0 Liquefied mass 16.4 ± 3.6 1.9 ± 9.3 25.9 ± 1.3 54.4 ± 3.1 1.3 ± 7.9 Fermented mass 15.9 ± 4.0 2.2 ± 5.8 25.9 ± 1.3 54.8 ± 2.2 1.3 ± 3.5 Whole stillage 15.8 ± 4.0 2.2 ± 5.4 26.0 ± 2.0 54.6 ± 2.0 1.3 ± 1.6 Thin stillage 15.3 ± 6.2 2.3 ± 6.5 27.1 ± 1.1 54.2 ± 1.9 1.1 ± 3.3 Distillers soluble 15.8 ± 6.5 2.3 ± 4.5 26.7 ± 2.1 54.0 ± 1.4 1.2 ± 4.7 Distillers grains 16.5 ± 2.8 2.2 ± 3.7 25.3 ± 2.2 54.7 ± 1.5 1.4 ± 1.2 DDGS 16.2 ± 3.4 2.3 ± 2.7 25.6 ± 1.3 54.5 ± 1.7 1.4 ± 1.8 Average ± CV ; Adapted from Moreau et al. 2011. Table 4. Free fatty acid content in corn and corn co-products (% fresh weight basis) Corn DDG DDGS TS CDS Plants (N) 1 5 7 2 1 Average 2.28 7.41 9.13 9.36 10.1 SD 0.02 0.78 1.49 0.63 NA DDG = distillers dried grains; DDGS = distillers dried grains with solubles;TS = thin stillage; CDS = corn distillers solubles; NA = not available. Adapted from Moreau et al. (2011). Table 5. Fat composition from different corn co-products (% fresh weight basis). SFFA UFFA TFFA G1 G2 G3 DG 1.8±0.2 5.6±0.4 7.4 0.7±0.2 0.6±0.2 91.1±0.3 WS 1.7±0.1 5.9 ±0.3 7.6 0.6±0.1 0.6±0.3 91.3±0.2 CDS 1.5±0.3 5.9±-0.4 7.4 0.5±0.2 0.4±0.4 91.1±0.5 Average ± SD. FFA = saturated free fatty acids; UFFA = unsaturated free fatty acids;TFFA = total free fatty acids; G1 = monoglycerides; G2 = diglycerides; G3 = triglycerides; WS = whole stillage; CDS = corn distillers solubles. Adapted from Noureddini et al. (2009).
  • 6. Page 6 iGrow | A Service of SDSU Extension | livestock Table 7. Nutrient composition of low fat distillers grains (LFDDG) (% dry basis) Solaris Verasun Dakota Ethanol FWS POET 6% POET 8% DM, as-fed 90.0 87.2 88.1 NA 90.2 90.36 CP 30.0 34.7 30.1 35-37 31.2 31.34 ADF NA 25.3 NA NA 10.5 10.04 NDF NA 31.6 29.0 21 27.6 29.29 Ash 2.50 5.26 4.66 3.8 5.71 5.70 EE 2.50 3.85 7.55 6.50 6.16 8.17 Ca NA 0.10 0.03 NA 0.07 0.08 P NA 0.83 0.92 NA 1.09 1.07 DM = dry matter; CP = crude protein; ADF = acid detergent fiber; NDF = neutral detergent fiber; EE = ether extract; Ca = calcium; P = phosphorus; NA = not available; POET = compilation of values from Lhrormann. 2006; VERASUN= compilation of values from Jacela. 2011, Mjoun et al. 2010 a,c: DAKOTA ETHANOL= Personal communication (unpublished); FWS=FWS (2011); POET= Personal communications (unpublished). Table 6. Nutrient composition of high protein distillers grains (HPDDG) (% dry basis) POET Solaris MorTechnology Renessen Brand Name Dakota Gold HPDDG Glutenol DM, as-fed 91.7 90 94.09 NA CP 43.5 47.75 53.39 NA ADF 10.9 6.7 27.3 35-50 NDF 26.1 34.1 34 7.0-11.0 Ash 2.5 4.65 NA 15-25 EE 3.8 3.55 3.48 NA Ca 0.02 0.07 0.16 4 P 0.91 0.67 0.32 NA DM = dry matter; CP = crude protein; ADF = acid detergent fiber; NDF = neutral detergent fiber; EE = ether extract; Ca = calcium; P = phosphorus; NA = not available; POET = compilation of values from Depenbusch et al. 2008; Robinson et al. 2008; Widmer et al. 2008; Tedeschi et al. 2009; Abdelqader et al. 2009; Mjoun et al. 2010b; Christen et al 2010; Kelzer et al. 2010. SOLARIS = compilation of values from: Lohrmann 2006; MOR TECH.= Applegate et al. 2009; RENESSEN = Stern. 2007.
  • 7. Page 7 iGrow | A Service of SDSU Extension | livestock Bibliografía Abdelqader, M.M., A.R., Hippen, K.F. Kalscheur, D.J. Schingoethe, A.D. Garcia. 2009. Isolipidic additions of fat from corn germ, corn distillers grains, or corn oil in dairy cow diets. J. Dairy Sci., 92: 5523-5533. Anderson, J.L., D.J . Schingoethe, K.F. Kalscheur, A.R. Hippen. 2006. Evaluation of dried and wet distillers grains included at two concentrations in the diets of lactating dairy cows. J. Dairy Sci., 89:3133-3142 Applegate, T. J., C. Troche, Z. Jiang, T. Johnson. 2009. The nutritional value of high-protein corn distillers dried grains for broiler chickens and its effect on nutrient excretion. Poultry Sci.,88:354–359. Bauman, D.E., J. M. Griinari. 2001. Regulation and nutritional manipulation of milk fat: low-fat milk syndrome. Livest. Prod. Sci., 70:15–29 Belyea, R. L., K. D. Rausch, and M. E. Tumbleson. 2004. Compositionof corn and distillers dried grains with solubles from dry grind ethanol processing. Bioresour. Technol., 94:293–298. Chalupa, W., B. Rickabaugh, D. S. Kronfeld, and D. Sklan. 1984. Rumen fermentation in vitro as influenced by long chain fatty. J. Dairy Sci., 67:1439-1444. Christen, K.A., D.J .,Schingoethe, K.F., Kalscheur, A.R. Hippen, K. Karges, M. L. Gibson. 2010. Response of lactating dairy cows to high protein distillers grains or three other protein supplements. J. Dairy Sci., 93: 2095-2104. Dairy One Forage Lab. 1Valores acumulados desde el 01/05/2000 hasta el 30/04/2011. Disponible online: http://www.dairyone.com . Acceso noviembre 2011. Depenbusch, B.E., E.R. Loe, M.J. Quinn, M.E. Corrigan, M.L. Gibson, K.K. Karges, J.S. Drouillard. 2008. “Corn Distiller’s Grain with Solubles Derived from a Traditional or Partial Fractionation Process: Growth Performance and Carcass Characteristics of Finishing Feedlot Heifer.” J. Anim. Sci., 86:2338–2343. Ganesan, V., K.A. Rosentrater, K. Muthukumarappan. 2005. “Effect of Moisture Content and Soluble Levels on the Physical and Chemical Properties of DDGS.” ASAE Paper No. 056110. American Society of Agricultural and Biological Engineers, St. Joseph, MI. Griinari, J. M., D.A. Dwyer, M.A., McGuire, D.E. Bauman, D.L. Palmquist, K. V. V. Nurmela. 1998. Trans-octadecenoic acids and milk fat depression in lactating dairy cows. J. Dairy Sci., 81:1251–61 FEDNA (Federacion Espanola para el Desarrollo de la Nutricion Animal). 2003. Tablas FEDNA de composición y valor nutritivo de alimentos para la fabricación de piensos compuestos (2ª edición). Madrid, España. 423 pp. FWS. 2011. The FWS corn fractionation system: Are you getting only a fraction of your profit potential? Disponible online: http:// www.fwstl.com/pdfs/fwst_presentation.pdf. Acesso en noviembre de 2011. INRA (Institut National de la Reserche Agronomique). 2004. Tables of composition and nutritional value of feed materials. Wageningen, Netherlands, Wageningen Academic Publishers. 304 pp. Jacela, J. Y., J. M. DeRouchey, S. S. Dritz, M. D. Tokach, R. D. Goodband, J. L. Nelssen, R. C. Sulabo, R. C. Thaler, L. Brandts, D. E. Little and K. J. Prusa. 2011. Amino acid digestibility and energy content of deoiled (solvent-extracted) corn distillers dried grains with solubles for swine and effects on growth performance and carcass characteristics. J. Anim. Sci. 89:1817–1829. Jenkins, T. C. 1993. Symposium: Advances in ruminant lipid metabolism. J. Dairy Sci., 76:3851-3863 Jenkins, T. C., C. M. Klein, G. D. Mechor. 2009. Managing Milk Fat Depression: Interactions of Ionophores, Fat Supplements, and other Risk Factors. Proceeding 20 TH Annual Florida Ruminant Nutrition Symposium. Gainesville, Florida. Kalscheur, K.F. 2005. Impact of feeding distillers grains on milk fat, protein, and yield. Proc. Distillers Grains Technology Council, 9th Annual Symposium, Louisville, Kentucky, USA. Kelzer, J.M., P.J. Kononoff, A.M. Gehman, L.O.Tedeschi, K. Karges, M. L. Gibson. 2009. Effects of feeding three types of corn-milling co-products on milk production and ruminal fermentation of lactating Holstein cattle. J. Dairy Sci., 92: 5120-5132.
  • 8. Page 8 iGrow | A Service of SDSU Extension | livestock Lhrormann, T. 2006. Rethinking ethanol coproducts. Distillers Grains Quarterly 1(4): 23- 24. Martinez Amezcua, C., C.M. Parsons, and S.L. Noll. 2004. “Content and Relative Bioavailability of Phosphorus in Distillers Dried Grains with Solubles in Chicks.” Poultry Sci., 83: 971-976. Mjoun, K., K.F. Kalscheur, A.R. Hippen., D.J. Schingoethe. 2010a. Performance and amino acid utilization of early lactating cows fed regular or reduced-fat dried distillers grains with solubles. J. Dairy Sci., 93: 3176-3191. Mjoun, K., K.F. Kalscheur, A.R. Hippen., D.J. Schingoethe. 2010b. Ruminal degradability and intestinal digestibility of protein and amino acids in soybean and corn distillers grains products. J. Dairy Sci., 93: 4144-4154. Mjoun, K., K.F. Kalscheur, A.R. Hippen., D.J. Schingoethe, D.E. Little. 2010c. Lactation performance and amino acid utilization of cows fed increasing amounts of reduced-fat dried distillers grains with solubles. J. Dairy Sci., 93: 288-303 Moureau, R. A., K. Liu., J. K. Winkler-Moser, V. Singh. 2011. Changes in Lipid Composition During Dry Grind Ethanol Processing of Corn. J. Am. Oil Chem. Soc., 88:435–442. NASS (National Agricultural Statistics Service Agricultural Statistics Board, U.S.). Department of Agriculture (2007). Ethanol Co- Products Used for Livestock Feed. National Research Council. 2001. Nutrient Requirements for Dairy Cattle. 7th rev. ed. Natl. Acad. Sci., Washington, DC, USA. Noll, S., C. Parsons, B. Walters. 2006. “What’s New since September 2005 in Feeding Distillers Co-products to Poultry.” Proceedings of the 67th Minnesota Nutrition Conference and University of Minnesota Research Update Session: Livestock Production in the New Millennium, pp. 149-154. Noureddini, H., S. R. P. Bandlamudi, E. A. Guthrie. 2009. A Novel Method for the Production of Biodiesel from the Whole Stillage- Extracted Corn Oil. J. Am. Oil Chem. Soc., 86:83–91 Nyoka, R. 2010. Fat and rumen undegradable protein for grazing cows: effect on composition of milk and quality of cheese. M.Sc. Thesis. South Dakota State University. Owens, F. (2009). Nutrition Health: Dairy. Cattle nutritionists. Feedstuffs, pg 22 – 25. Owens, T.M. 2009. Risk of milk fat depression for dairy cows fed high moisture corn and distillers grains in diets containing monensin. Ph.Sc. Thesis. South Dakota State University. Quettier, A. L., P. J. Eastmond. 2009. Storage oil hydrolysis during early seedling growth. Plant Physiology and Bioch. 47 (6):485 – 490. QTI. 2011. Quality Technology International. Glutenol for ruminants. Disponible online en: http://www.qtitechnology.com/PDF%20Files/Glutenol%20for%20Ruminants%202-09.pdf. Acceso en noviembre de 2011. Ranathunga, S.D., K.F. Kalscheur, A.R. Hippen., D.J. Schingoethe. 2010. Replacement of starch from corn with nonforage fiber from distillers grains and soyhulls in diets of lactating dairy cows. J. Dairy Sci., 93: 1086-1097. Robinson, P. H., K. Karges, M.L. Gibson. 2008. Nutritional evaluation of four co-product feedstuffs from the motor fuel ethanol distillation industry in the Midwestern USA. Anim. Feed Sci. and Tech., 146:345-352. Rosentrater, K. A., K.M. Ileleji, D. B. Johson. 2011. Manufacturing of Fuel Ethanol and Distiller Grains – Current and Evolving Processes. Capitulo 5. Distillers Grains: Production, Properties, and Utilization. Editado por KeShun Liu and Kurt A. Rosentrater. CRC Press, Florida. Pg. 73 – 102. Spiehs, M. J., M. H. Whitney, and G. C. Shurson. 2002. Nutrient database for distillers dried grains with solubles produced from new ethanol plants in Minnesota and South Dakota. J. Anim. Sci., 10:2639-2645. Stern, M. 2007. The Renessen Corn Processing System: Rebalancing the Bioenergy/Feed Equation. Innovation to Commercialization. Chicago, Illinois; Illinois Biotechnology Industry Organization. Tang, S. C., I. Zulkifli, M. Ebrahimi, A.R. Alimon, A.F. Soleimani, K. Filer. 2011. Effects of Feeding Different Levels of Corn Dried Distillers Grains with Solubles on Growth Performance, Carcass Yield and Meat Fatty Acid Composition in Broiler Chickens. Int. J. Anim. Vet. Adv., 3(3): 205-211.
  • 9. Page 9 iGrow | A Service of SDSU Extension | livestock Publication: 02-2003-2012 South Dakota State University, South Dakota counties, and U.S. Department of Agriculture cooperating. South Dakota State University is an Affirmative Action/Equal Opportunity Employer and offers all benefits, services, education, and employment opportunities without regard for race, color, creed, religion, national origin, ancestry, citizenship, age, gender, sexual orientation, disability, or Vietnam Era veteran status. Tedeschi, L. O., P. J. Kononoff, K. Karges, and M. L. Gibson. 2009. Effects of chemical composition variation on the dynamics of ruminal fermentation and biological value of corn milling (co) products. J. Dairy Sci., 92:401–413. UMN. 2009. Distillers Grains By-products in Livestock and Poultry Feeds. University of Minnesota. U.S. average compared to DDGS produced in Canada, China, Spain. Disponible online: http://www.ddgs.umn.edu/profiles.htm Widmer, M.R., L.M. McGinnis, D.M. Wulf, and H.H. Stein. 2008. “Effects of Feeding Distillers Dried Grains with Solubles, High Protein Distillers Dried Grains, and Corn Germ to Growing-Finishing Pigs on Pig Performance, Carcass Quality, and the Palatability of Pork.” J. Anim. Sci. 1910. doi: 10.2527/ jas.2007-0594. Winkler-Moser, J. K. 2011. Lipids in DDGS. Capitulo 9. Distillers Grains: Production, Properties, and Utilization. Editado por KeShun Liu and Kurt A. Rosentrater. CRC Press, Florida. Pg. 73 – 102.