1. Elvira Sukamtoh
University of Massachusetts Amherst
IFTSA Undergraduate Research Competition
© 2015 Institute of Food Technologists
Curcumin Inhibits Proliferation of Human Lymphatic
Endothelial Cells Through G2 Cell Cycle Arrest

2. Curcumin inhibits tumor metastasis
© 2015 Institute of Food Technologists
►Tumor metastasis causes ~90%
cancer deaths.
►Curcumin has anti-metastatic
effects.
►HOWEVER, the mechanisms
are poorly understood.
Curcuma longa
(Turmeric)
O O
HO OH
OCH3H3CO
Curcumin
Life Sciences (2006): 2081-087

3. Lymphangiogenesis:
A crucial mechanism for tumor metastasis
© 2015 Institute of Food Technologists
Nature Reviews Cancer (2014): 159-72
Formation of new lymphatic vessels

4. Lymphangiogenesis:
A crucial mechanism for tumor metastasis
© 2015 Institute of Food Technologists
Nature Reviews Cancer (2014): 159-72

5. Lymphangiogenesis:
A crucial mechanism for tumor metastasis
© 2015 Institute of Food Technologists
O O
HO OH
OCH3H3CO
Curcumin

6. Primary Human Lymphatic Endothelial Cell
© 2015 Institute of Food Technologists
HMVEC-dLy
(Human dermal vascular lymphatic endothelial cells)
Nature Reviews Cancer (2014): 159-72

7. Cell Proliferation Procedure
*HMVEC-dLy
Curcumin
© 2015 Institute of Food Technologists
MTT AssayPlate Treatment
*HMVEC-dLy : Human dermal vascular lymphatic endothelial cells

8. Cell Proliferation Results:
Curcumin inhibits proliferation of HMVEC-dLy
0
20
40
60
80
100
120
140
Ctrl 4.5 6 7.5 9 10.5 12 13.5 15
CellProliferation(%toCtrl)
Curcumin Treatment (µM)
0
20
40
60
80
100
120
140
Ctrl 4.5 6 7.5 9 10.5 12 13.5 15
CellProliferation(%toCtrl)
Curcumin Treatment (µM)
24h 48h
*P<0.05, the results are mean ± SD.
Results are from at least 3 independent repeats.
*
*
*
* * * * *
© 2015 Institute of Food Technologists

9. Cell Cycle Procedure
Curcumin
© 2015 Institute of Food Technologists
* HMVEC-dLy
Flow Cytometer
Treatment
Collect
*HMVEC-dLy : Human dermal vascular lymphatic endothelial cells

10. Cell Cycle Results:
Curcumin causes G2 cell cycle arrest
© 2015 Institute of Food Technologists
*P<0.05, the results are mean ± SD.
Results are from at least 3 independent repeats.
Control Curcumin
(10 µM)
Raw figures from the flow cytometer
G1 S G2
Cellpopulation(%)
0
20
40
60
80
100
Control
Curcumin
*
Data Analysis

11. Cell Cycle Results:
Curcumin causes G2 cell cycle arrest (cont’d)
© 2015 Institute of Food Technologists
https://upload.wikimedia.org/wikipedia/
commons/f/f7/Cell_cycle_simple.png
O O
HO OH
OCH3H3CO
Curcumin

12. Conclusion
Tube
Formation Cell
Proliferation
Cell
Migration
O O
HO OH
OCH3H3CO
Curcumin
© 2015 Institute of Food Technologists
Wang W. (#), Sukamtoh E. (#), Xiao H., and Zhang G. Curcumin inhibits lymphangiogenesis in
vitro and in vivo. Revision submitted to Molecular Nutrition & Food Research.
(#) co-first author

13. Conclusion
© 2015 Institute of Food Technologists
O O
HO OH
OCH3H3CO
Curcumin
Wang W. (#), Sukamtoh E. (#), Xiao H., and Zhang G. Curcumin inhibits lymphangiogenesis in
vitro and in vivo. Revision submitted to Molecular Nutrition & Food Research.
(#) co-first author

14. Future Plan
►Investigate the lymphangiogenesis inhibition of curcumin
in tumor model.
© 2015 Institute of Food Technologists

15. Elvira Sukamtoh
esukamto@umass.edu
© 2015 Institute of Food Technologists
Thank you!
Acknowledgements:
Guodong Zhang1
Weicang Wang1
Zhang Lab Members1
1Department of Food Science, University of Massachusetts Amherst