Top-Selling ELISA Kits 2016 - Elabscience

Elabscience®
Focus on lab research Service for life science
ELISA
KIT
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Elabscience specializes in immunodiagnostic technology for the
Life Science community. We have complete R&D and manufacture
platform and have good quality control for every kit by every lot.
We endeavor to reduce lot to lot variation to keep your experiment
results more consistent and precise. Through unremitting efforts
and development, Elabscience’s products have gained more
market share, customers are now all over the world in more than
100 countries.Our major products are Proteins, Antibodies, ELISA
Kits, CLIA Kits, Labeling Kits and related reagents.Elabscience
also provides custom services for our customers including Protein
Expression and Purification Service, Antibody Preparation Service,
ELISA Customized Service, Cell Biology Service, Molecular Biology
Service and Whole Research Project Solutions.
About Elabscience
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Focus on Lab Research, Service for Life Science.
Application areas
Why choosing Elabsience?
Advantages:
High Stability
Full Range of Products
Efficient Delivery
Reliable Supply Chain
Perfect Technical Support and After-sale Service
Autoimmunity
Enzyme & Kinase
Endocrinology
Immunomodulator
Apoptosis
Cardiovascular disease
Cancer
Hormone
Globulins
Hematology
Hypersensitivity
Respiratory system
Cytokines & Growth
Factors
Innate immunity
Development
Infectious Diseases
Urinary system
Stem cell
Digestive system
Metabolism
Neuroscience
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Cat.No Product Name Detection Range Sensitivity
E-EL-H0543 Human Aβ1-42 (Amyloid Beta Peptide 1-42) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H0010 Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H0483 Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit 12.5--800pg/mL 7.5pg/mL
E-EL-H0622 Human CACYBP (Calcyclin Binding Protein) ELISA Kit 78.125--5000pg/mL 46.875pg/mL
E-EL-H0130 Human COL1 (Collagen Type I) ELISA Kit 0.625--40ng/mL 0.375ng/mL
E-EL-H0851 Human CPP (Copeptin) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H0043 Human CRP (C-Reactive Protein) ELISA Kit 0.781--50ng/mL 0.469ng/mL
E-EL-H0835
Human CTXI (Cross Linked C-telopeptide of Type I Collagen)
ELISA Kit
0.156--10ng/mL 0.094ng/mL
E-EL-H0864 Human D2D (D-Dimer)ELISA Kit 78.125--5000ng/mL 64.875ng/mL
E-EL-H0059 Human EGF (Epidermal Growth Factor) ELISA Kit 3.906--250pg/mL 2.344pg/mL
E-EL-H0064 Human ET-1 (Endothelin 1) ELISA Kit 1.25--80pg/mL 0.75pg/mL
E-EL-H2237 Human GC (Glucagon) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H1908 Human GDF11 (Growth Differentiation Factor 11) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H0177 Human GH (Growth Hormone) ELISA Kit 78.125--5000pg/mL 64.875pg/mL
E-EL-H0148 Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit 0.313--20ng/mL 0.188ng/mL
E-EL-H0081
Human GM-CSF (Granulocyte Macrophage Colony Stimulating
Factor) ELISA Kit
7.813--500pg/mL 4.688pg/mL
E-EL-H5497 Human Hepc25 (Hepcidin 25) ELISA Kit 1.563--100ng/mL 0.938ng/mL
E-EL-H1554 Human HMGB-1(High Mobility Group Protein B1)ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H2532 Human IFN-α (Interferon Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H0085 Human IFN-β(Interferon Beta)ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H0108 Human IFN-γ (Interferon Gamma) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H2161 Human IgE (Immunoglobulin E) ELISA Kit 3.125--200ng/mL 1.875ng/mL
E-EL-H0086 Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit 1.563--100ng/mL 0.938ng/mL
E-EL-H0103 Human IL-10 (Interleukin 10) ELISA Kit 7.813--500pg/mL 4.688pg/mL
E-EL-H0149 Human IL-1β (Interleukin 1 Beta) ELISA Kit 7.813--500pg/mL 4.688pg/mL
E-EL-H0099 Human IL-2 (Interleukin 2) ELISA Kit 125--8000pg/mL 75pg/mL
Top-selling Kits
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E-EL-H0113 Human LEP (Leptin) ELISA Kit 78.125--5000pg/mL 46.875pg/mL
E-EL-H0020 Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H0134 Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit 0.313--20ng/mL 0.188ng/mL
E-EL-H1446 Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-H1451 Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H2016 Human MT (Melatonin) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H1386 Human NFκB (Nuclear Factor Kappa B) ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-H0096
Human NGAL (Neutrophil Gelatinase Associated Lipocalin)
ELISA Kit
0.156--10ng/mL 0.094ng/mL
E-EL-H1893 Human NPY (Neuropeptide Y) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H1047 Human NSE (Neuron-Specific Enolase) ELISA Kit 2.344--150ng/mL 1.406ng/mL
E-EL-H2234 Human PI (Proinsulin) ELISA Kit 0.313--20ng/mL 0.188ng/mL
E-EL-H0183
Human PIIINP (N-Terminal Procollagen III Propeptide)
ELISA Kit
23.438--1500pg/mL 14.063pg/mL
E-EL-H1476 Human PTH (Parathyroid Hormone) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H1297 Human S100B (S100 Calcium Binding Protein B) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H1487 Human TG (Thyroglobulin) ELISA Kit 125--8000ng/mL 75ng/mL
E-EL-H0110 Human TGF-β1 (Transforming Growth Factor Beta 1)ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H0109 Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 7.813--500pg/mL 4.688pg/mL
E-EL-H0111
Human VEGF-A (Vascular Endothelial Cell Growth Factor A )
ELISA Kit
31.25--2000pg/mL 18.75pg/mL
E-EL-R0048 Rat ACTH (Adrencocorticotropic Hormone) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-R0329 Rat ADP/Acrp30 (Adiponectin) ELISA Kit 46.875--3000pg/mL 28.125pg/mL
E-EL-R1430 Rat ANG II (Angiotensin II) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-R0126 Rat BNP (Brain Natriuretic Peptide) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-R0135 Rat CGRP (Calcitonin Gene Related Peptide) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-R0269 Rat CORT (Corticosterone) ELISA Kit 78.125--5000pg/mL 46.875pg/mL
E-EL-R0007 Rat EPO (Erythropoietin) ELISA Kit 62.5--4000pg/mL 37.5pg/mL
E-EL-R0167 Rat ET-1 (Endothelin 1) ELISA Kit 7.813--500pg/mL 4.688pg/mL
E-EL-R0391 Rat FSH (Follicle Stimulating Hormone) ELISA Kit 3.125--200ng/mL 1.875ng/mL
E-EL-R0029 Rat GH (Growth Hormone) ELISA Kit 0.313--20ng/mL 0.188ng/mL
E-EL-R0506 Rat hs-CRP (High Sensitivity C-Reactive Protein) ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-R0009 Rat IFN-γ (Interferon Gamma) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-R0016 Rat IL-10 (Interleukin 10) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
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E-EL-R0011 Rat IL-1α (Interleukin 1 Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-R0012 Rat IL-1β (Interleukin 1 Beta) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-R2466 Rat INS (Insulin) ELISA Kit 3.125--200ng/mL 1.875ng/mL
E-EL-R0582 Rat LEP (Leptin) ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-R0026 Rat LH (Luteinizing Hormone) ELISA Kit 1.563--100mIU/mL 0.938mIU/mL
E-EL-R0025 Rat MAU (Microalbuminuria) ELISA Kit 1.563--100μg/mL 0.938μg/mL
E-EL-R0031 Rat MT (Melatonin) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-R0520 Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible)ELISA Kit 0.313--20ng/mL 0.188ng/mL
E-EL-R0243 Rat OC/BGP (Osteocalcin) ELISA Kit 0.781--50ng/mL 0.469ng/mL
E-EL-R2400 Rat PCT (Procalcitonin) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-R0046
Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1)
ELISA Kit
0.391--25ng/mL 0.234ng/mL
E-EL-R0084 Rat TGF-β1(Transforming Growth Factor β1) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-R0540 Rat TIMP-1(Tissue Inhibitors of Metalloproteinase 1)ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-R0019 Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 78.125--5000pg/mL 46.875pg/mL
E-EL-R0054 Rat Tn-T (Troponin T) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M1272 Mouse ALT (Alanine Transaminase) ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-M0160 Mouse AST (Aspartate Aminotransferase) ELISA Kit 0.156--10ng/mL 0.094ng/mL
E-EL-M0068 Mouse Aβ1-42 (Amyloid Beta 1-42) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0339 Mouse C5a (Complement Component 5a) ELISA Kit 62.5--4000pg/mL 37.5pg/mL
E-EL-M0053 Mouse CRP (C-Reactive Protein) ELISA Kit 0.391--25ng/mL 0.234ng/mL
E-EL-M0027 Mouse EPO (Erythropoietin) ELISA Kit 78.125--5000pg/mL 64.875pg/mL
E-EL-M0090 Mouse GLP-1 (Glucagon Like Peptide 1)ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0676 Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0713 Mouse IFN-α (Interferon Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0033 Mouse IFN-β(Interferon Beta)ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0048 Mouse IFN-γ (Interferon Gamma) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0690 Mouse IgA (Immunoglobulin A) ELISA Kit 6.25--400ng/mL 3.75ng/mL
E-EL-M0034 Mouse IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit 78.125--5000pg/mL 64.875pg/mL
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E-EL-M0692 Mouse IgG (Immunoglobulin G) ELISA Kit 1.563--100ng/mL 0.938ng/mL
E-EL-M0046 Mouse IL-10 (Interleukin 10) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0037 Mouse IL-1β (Interleukin 1 Beta) ELISA Kit 7.813--500pg/mL 4.688pg/mL
E-EL-M2614 Mouse INS(Insulin) ELISA Kit 3.125--200ng/mL 1.875ng/mL
E-EL-M0039 Mouse LEP (Leptin) ELISA Kit 125--8000pg/mL 75pg/mL
E-EL-M0006 Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0627 Mouse MMP-9 (Matrix Metalloproteinase-9) ELISA Kit 78.125--5000pg/mL 64.875pg/mL
E-EL-M0864 Mouse OC/BGP (Osteocalcin) ELISA Kit 0.391--25ng/mL 0.234ng/mL
E-EL-M0051 Mouse TGF-β1 (Transforming Growth Factor β1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-M0641
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
ELISA Kit
0.156--10ng/mL 0.094ng/mL
E-EL-M0049 Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-Ch0026 Chicken IFN-γ (Interferon Gamma) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-Ch1621 Chicken IL-10 (Interleukin 10) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-Ch0228 Chicken IL-6(Interleukin 6) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-P0003 Porcine IFN-γ (Interferon Gamma) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-P0004 Porcine IgG (Immunoglobulin G) ELISA Kit 3.125--200ng/mL 1.875ng/mL
E-EL-P0020 Porcine IL-6 (Interleukin 6) ELISA Kit 125--8000pg/mL 75pg/mL
E-EL-P0010 Porcine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-GP0002 Guinea pig IgG (Immunoglobulin G) ELISA Kit 1.563--100ng/mL 0.938ng/mL
E-EL-MK2266 Monkey CIC(Circulating Immune Complex)ELISA Kit 0.313--20ug/mL 0.188ug/mL
E-EL-RB0011 Rabbit TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-0028 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit 1.563--100ng/mL 0.938ng/mL
E-EL-0056 cAMP (Cyclic Adenosine Monophosphate) ELISA Kit 1.563--100ng/mL 0.938ng/mL
E-EL-0083 cGMP(Cyclic GMP)ELISA Kit 0.781--50pmol/mL 0.469pmol/mL
E-EL-0030 Cortisol ELISA Kit 12.5--800ng/mL 7.5ng/mL
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E-EL-H0137 Human ACTH (Adrenocorticotropic Hormone) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-H0004 Human ADP/Acrp30 (Adiponectin) ELISA Kit 0.781--50ng/mL 0.469ng/mL
E-EL-H0317 Human AMH (Anti-Mullerian Hormone) ELISA Kit 93.75--6000pg/mL 56.25pg/mL
E-EL-H0008 Human ANG2 (Angiopoietin 2) ELISA Kit 46.875--3000pg/mL 28.125pg/mL
E-EL-H0326 Human Ang-II (Angiotensin II) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-H0542 Human Aβ1-40 (Amyloid Beta Peptide 1-40) ELISA Kit 15.625--1000pg/mL 9.375pg/mL
E-EL-0065 E2 (Estradiol) ELISA Kit 21.875--1400pg/mL 13.125pg/mL
E-EL-0045 EPI (Epinephrine/Adrenaline) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
E-EL-0067 SP (Substance P) ELISA Kit 78.125--5000pg/mL 46.875pg/mL
E-EL-0072 T (Testosterone) ELISA Kit 0.313--20ng/mL 0.188ng/mL
E-EL-0046 DA (Dopamine) ELISA Kit 31.25--2000pg/mL 18.75pg/mL
1.Some tips for ELISA failure
2.Poor Precision in ELISA experiment
ELISA failure can be attributed to many factors. Many unexpected results can be avoided if the manual is
read and fully understood before starting the experiment. On identifying assay failure, you can follow tips below.
1.  Check the expiration dates of the kit, ensure that all the reagents have been stored as indicated in the
manual.
2.  Check for signs of instability or deterioration in reagent solutions, such as precipitation or discoloration and
so on.
3.  Substrate reagent should be colorless.
4.  Use clean plastic disposable pipettes, tips, and containers for reagent preparation and storage. Avoid
cross-contamination of reagents by changing pipette tips between addition of each standard, sample and reagent.
5.  Ensure that specified incubation times and temperatures have been adhered to.
6.  Ensure that no substitution of kit reagents has occurred.
7.  It is recommended that samples and standards be run in duplicate to improve accuracy.
Possible causes Solutions
Incomplete washing of wells
Inadequate aspiration of wells
Ensure that wash apparatus is working correctly
Ensure adequate time of soaking
Wells should appear dry after aspiration
Frequently Asked Questions
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3.Poor Standard Curve in ELISA experiment
4.High Background in ELISA experiment
5.Too much signal in ELISA experiment
6.Standard curve achieved but poor discrimination
Possible causes
Possible causes
Possible causes
Possible causes
Solutions
Solutions
Solutions
Solutions
Improper dilution of standard
Insufficient washing
Incomplete washing of wells
Not enough HRP-Conjugate
Unequal mixing of reagents
Unequal mixing of reagents
Improper calculation of standard curve
dilutions
Incomplete washing
Plate not incubated long enough
Pipetting error
Unequal volumes added to wells
Pipetting error
Use diluent in the kit as blank value.
Ensure accurate dilution of highest standard
Ensure accurate completion of 2-fold dilution series
See washing procedures in the manual
Increase soaking time between washes
Ensure adequate time of soaking
Ensure correct dilution
Ensure adequate mixing
Ensure adequate mixing
Check calculations, make new standard curve
Increase Substrate Solution incubation time
Check calculations, make new standard curve
Check pipette function;
Recalibrate the pipetter if necessary
Set duplicate in each assay
7.Poor Duplicates in ELISA experiment
Possible causes Solutions
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9.Very low readings across the plate in ELISA experiment
10.How to wash the ELISA plate?
8.Samples ' OD are too high, but standard curve looks fine
Possible causes
Possible causes
Solutions
Solutions
Incorrect wavelength
Samples contain determinant levels above
the kit’s detection range
No plate sealers used
Insufficient incubation time
Incorrect dilutions of detection Ab/HRP-
conjugate
Stop solution not added
Buffers contaminated
Incubation temperature vary wildly
The kit has lost its activity
Check filters/reader
Dilute samples and run again
No plate sealers
Increase incubation time
Adhere to the manual
Add stop solution to each well
Make fresh buffers
Check the incubator
Get a new kit
Correct washing of the wells is very crucial for a successful ELISA. Deionized or distilled water should be
used when diluting the concentrated Wash Buffer.
Using multi-channel pipette
Firstly, check the strips in the plate so that they are firmly in the plate holder. Numbering the strips may be
useful in the event that strips become loose while decanting. Secondly, empty the liquid in the plate by decanting.
Fill each well with the volume of Wash Buffer specified in the kit manual. Set a timer for the recommended time
and let the Wash Buffer soak in the wells. Decant the wells by inverting the plate and rapping it against clean paper
toweling. Repeat this process as directed in the kit manual. After the last decanting, remove any remaining Wash
Buffer by inverting the plate and blotting it dry by rapping it firmly against clean paper toweling on a hard surface.
Do not let the wells to sit dry. Proceed immediately to the next step in the kit manual.
Using manifold dispenser or autowasher
Equip the dispenser or autowasher with an appropriate vacuum supply (refer to manufacturer’s guidelines).
Ensure that each cannulae is dispensing and aspirating properly. Set the volume of Wash Buffer in each well
and soaking time for each washing. Then put the plate in. Ensure that no Wash Buffer is left in the wells after
aspiration. Do not over-aspirate the wells by allowing the aspiration apparatus to sit in the wells after the aspiration.
Plate sealer reused Use a fresh plate sealer for each step
Insufficient washing Ensure sufficient washing
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11.Pipetting Tips for researchers
12. Calculation of ELISA results
The key to repeated accuracy in pipetting is a consistent technique. Consistent speed and smoothness is
necessary while pipetting. Avoid sudden motions when drawing or dispensing liquid.
1.  Check to be sure that the pipette tip is fitted properly on the end of the pipette and seated with adequate
pressure
2.  Change pipette tips between each sample or reagent addition.
3.  Do not pipette a lower volume than the manufacturer’s suggestion. This will decrease accuracy and
reproducibility.
4.  Some liquids will have a tendency to cling to the inside or outside surfaces of the pipette tips. To avoid
variations in the volumes drawn or dispensed caused by this surface tension, pre-rinse the pipette tip with the
sample or reagent. This will improve accuracy.
5.  If the reagent to be pipetted is viscous, wait a moment after drawing the liquid to allow the volume to reach
equilibrium before removing the pipette tip from the reservoir.
6.  After drawing the liquid into the tip, remove the tip from the solution and wipe the outside of the tip with a
lint-free tissue.
7.  If an air bubble appears in the tip while drawing the liquid, dispense the liquid back into the container and
re-pipette the liquid at a slower rate. If the air bubble appears a second time, discard the pipette tip and replace
with a new tip.
8.  If foaming occurs in the tip while drawing up the liquid, it may be necessary to hold the pipette at a slight
angle and draw the liquid slower.
9.  Use separate reservoirs for each reagent.
1. ELISA samples are always tested in duplicate or in triplicate, then average the absorbance values for each
set of standards and samples. Duplicates should be within 20% of the mean.
2. Subtract the average value of zero standard OD (optical density).
3. Standard curve.
Create a standard curve by plotting the mean absorbance for each standard on the x-axis against the
concentration on the y-axis and draw a best fit curve through the points on the graph. We suggest the suitable
software should be used for this, such as CurveExpert 1.3. The software is recommended that various methods (e.g.
linear, semi-log, log/log, 4 parameter logistics) be tried to see which curve best fits the data. One way to determine
if the curve is to backfit the standard curve O.D. values. Treat standards as unknowns and interpolate the O.D.
values from the standard curve. They should be close to the expected value (+/- 10%). Use the curve that gives
the best correlation (r) value and backfit.
Step by step as shown:
Washing the plate too rapidly or too slowly, incomplete washing or aspirating, and letting the wells to sit dry
are all factors that will affect the precision of the ELISA.
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11www.elabscience.cn
Step 1: Input information of the OD value and concentration of the standards
Step 2: Create and select a standard curve.
We recommend generating a standard curve for each set of samples assayed.
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Step 3: Calculate the concentration according to the curve
Input the OD value of the sample and click , then user can get the actual
concentration of target protein in the sample.
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13www.elabscience.cn
Or get the relevant equation
If the OD of the sample surpasses the upper limit of the standard curve, these samples should
be diluted before proceeding with the ELISA to obtain an accurate result. As for these samples, the
concentration obtained from the standard curve must be multiplied by the dilution factor when analyzing
the results.
Step 4: Samples that have an absorbance value falling out of the range of the standard curve
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14
Part of Published Articles
Cat.No Target Species Publications
E-EL-H0102
E-EL-H0109
E-EL-H0091
E-EL-H0102
E-EL-H5413
E-EL-H2050
E-EL-H0105
E-EL-H1064
E-EL-H1580
E-EL-H5574
Interleukin 6
Tumor Necrosis Factor
Alpha
Prostate Specific Antigen
Interleukin 6
Prostate specific
membrane antigen
Pepsin
Interleukin 17
Cytosolic Phospholipase
A2
Prostaglandin E2
Cytochrome C Oxidase
Subunit II
Human
Human
Human
Human
Liao Y, Liu P, Guo F, et al. Oxidative burst of circulating neutrophils
following traumatic brain injury in human.[J]. Plos One, 2013,
8(7):e68963. IF:3.234
Liu J, Lu C Y, Zhou H, et al. Flexible Gold Electrode Array for
Multiplexed Immunoelectrochemical Measurement of Three Protein
Biomarkers for Prostate Cancer[J]. Acs Applied Materials & Interfaces,
2014, 6(22):20137-20143. IF:6.723
Kang H S, Kim J K, Lee H J, et al. LRP1-dependent pepsin clearance
induced by 2′-hydroxycinnamaldehyde attenuates breast cancer cell
invasion[J]. International Journal of Biochemistry & Cell Biology, 2014,
53:15–23 IF:4.046
Sun Z, Wenbin L I, Hosipital B J S. Diet induced hyperhomocysteinemia
increases intestinal inflammation in an animal model of colitis.[J].
Journal of Crohn S & Colitis, 2015(2):58-60. IF:6.234
E-EL-H0101
E-EL-H0191
E-EL-H0104
Interleukin 4
Interleukin 5
Interleukin 13
Human
Li H T, Zhang T T, Chen Z G, et al. Intranasal administration of
CpG oligodeoxynucleotides reduces lower airway inflammation in a
murine model of combined allergic rhinitis and asthma syndrome[J].
International Immunopharmacology, 2015, 28(1):390-398. IF:2.427
E-EL-H0102
E-EL-H0180
E-EL-H0155
Interleukin 6
Interleukin 9
Cluster of
Differentiation 42
Human
Human
Human
Tang J N, Shen L, Liu C L, et al. Plasma Levels of C1q/TNF- or Stable
Angina Pectoris.[J]. American Journal of the Medical Sciences, 2015,
349(2):130-6. IF:1.389
Tuxun T, Apaer S, Ma H Z, et al. The Potential Role of Th9 Cell Related
Cytokine and Transcription Factors in Patients with Hepatic Alveolar
Echinococcosis[J]. Journal of Immunology Research, 2015, 2015(2):1-
7. IF:2.93
Yuan B, Chen Q, Ding W Q, et al. Copolymer coatings consisting of
2-methacryloyloxyethyl phosphorylcholine and 3-methacryloxypropyl
trimethoxysilane via ATRP to improve cellulose biocompatibility.[J]. Acs
Applied Materials & Interfaces, 2012, 4(8):4031-9. IF:6.723
E-EL-H1163
E-EL-H5475
E-EL-H0183
E-EL-H0196
E-EL-H0873
Elastin
C-telopeptide of
type Ⅰ collagen
N-Terminal Procollagen Ⅲ
Propeptide
Procollagen I C-terminal
Propeptide
Cross Linked C-telopeptide of
Type III Collagen
Human
Liu R, Chen L, Wei W, et al. Extracellular matrix turnover in coronary
artery ectasia patients.[J]. Heart & Vessels, 2015:1-9. IF:2.065
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15
Cat.No Targets Species Publications
E-EL-H0375
E-EL-H1675
E-EL-0060
E-EL-H1113
E-EL-H5410
E-EL-H5436
E-EL-H2532
E-EL-H0085
Fatty Acid Synthase
Proteoglycan 4
Malondialdehyde
Superoxide Dismutase 1,
Soluble
Glutathione Peroxidase 1
Reactive OXyen Species
Interferon Alpha
Interferon Beta
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Human
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Qin W, Raatz S, Zhang K K, et al. Polyunsaturated Fatty Acid content
may be increased in the milk of women with pregnancy-associated
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Journal of Oral Pathology & Medicine, 2014, 44(8):622–627. IF:1.926
Zhao, Jing, Xu, Shouzhu, Song, Fan. 2,3,5,4′-tetrahydroxystilbene-2-
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Yang Q, Fu S, Wang J. Hepatitis C virus infection decreases the
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Archives of Virology, 2014, 159(11):2997-3003. IF:2.39
E-EL-H1478
Parathyroid Hormone
Related Protein
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Nordholm A, Rix M, Olgaard K, et al. Parathyroid hormone-related
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E-EL-H0190
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E-EL-H1382
E-EL-H0148
E-EL-H0121
E-EL-H1779
E-EL-H1445
E-EL-H1451
E-EL-H2569
Complement Component 5a
Cross Linked
C-telopeptide of Type
II Collagen
Ribonuclease Inhibitor
Glucagon Like Peptide 1
C-Peptide
Urokinase-Type
Plasminogen Activator
Matrix Metalloproteinase 2
Vascular Endothelial Cell
Growth Factor
Human
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16
E-EL-H2340
E-EL-H0965
E-EL-H2447
E-EL-H5200
E-EL-H1163
E-CL-H1276
E-EL-H1539
E-EL-H0951
E-EL-H1655
E-EL-H1902
E-EL-H1858
4-Hydroxynonenal
Alpha 1-Antitrypsin
Peptidase Inhibitor 3, Skin
Derived
Lactoferrin
Elastin
Thymosin Beta 10
Toll-Like Receptor 4
Toll-Like Receptor 2
Mammalian Target of
Rapamycin
Epinephrine/Adrenaline
Noradrenaline/
Norepinephrine
Human
Human
Human
Liu R, Chen L, Wu W, et al. Neutrophil serine proteases and their
endogenous inhibitors in coronary artery ectasia patients.[J]. Anatolian
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the Efficacy of Cord Blood Cell Therapy for Cerebral Palsy.[J]. Journal
of Back & Musculoskeletal Rehabilitation, 2015, 24(19): 2259-2268.
IF:0.705
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2015, 2015(6). IF:3.236
E-EL-H0344
E-EL-0045
E-EL-0047
E-EL-H0185
E-EL-H0835
E-EL-H0572
E-EL-H1027
Cerebral Dopamine
Neurotrophic Factor
Epinephrine/Adrenaline
Noradrenaline/
Norepinephrine
Procollagen I N-terminal
Propeptide
Cross Linked C-telopeptide of
Type I Collagen
Beta-Endorphin
Superoxide Dismutase,
Soluble
Human
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E-EL-H1901 Nephrin Human
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endothelial growth factor in type 2 diabetes mellitus patients: a cross-
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E-EL-H1343 Osteocalcin Human
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E-EL-H1274 Semaphorin 3A Human
Wang Z, Chen J, Zhang W, et al. Axon guidance molecule
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IF:6.636
E-EL-H0108 Interferon Gamma Human
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IF:5.199
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E-EL-R0014
E-EL-R0016
E-EL-R0022
E-EL-R0019
E-EL-R0015
E-EL-R0011
E-EL-R0015
precursor of Prostate
Specific Antigen
Interleukin 4
Interleukin 10
C-Reactive Protein
Alpha
Interleukin 6
Interleukin 1 Alpha
Interleukin 6
Human
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E-EL-H2440
E-EL-H1554
E-EL-R0505
E-EL-R0011
E-EL-R0014
E-EL-R0016
E-EL-R0019
E-EL-R0014
E-EL-R0015
E-EL-R0559
E-EL-R0084
Amyloid Beta Peptide 1-42
Presenilin 1
High mobility group protein
B1
Interleukin 1 Alpha
Interleukin 4
Interleukin 10
Alpha
Interleukin 4
Interleukin 6
Interleukin 7
Transforming Growth
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E-EL-RB0022 Alkaline Phosphatase Rabbit
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E-EL-Ch0026
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Interferon Gamma
Interleukin 18
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E-EL-R0019
E-EL-R0020
E-EL-R0911
E-EL-R0582
E-EL-R2466
E-EL-R0329
E-EL-R0019
Alpha
Vascular Endothelial cell
Growth Factor
Vascular Endothelial Growth
Factor Receptor 1
Leptin
Insulin
Adiponectin
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Ahmed L A, Darwish H A, Abdelsalam R M, et al. Role of Rho Kinase
Inhibition in the Protective Effect of Fasudil and Simvastatin Against
3-Nitropropionic Acid-Induced Striatal Neurodegeneration and
Mitochondrial Dysfunction in Rats[J]. Molecular Neurobiology, 2015:1-
12. IF:5.137
E-EL-R0022
E-EL-R0015
E-EL-R0019
C-Reactive Protein
Interleukin 6
Alpha
Rat
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Alternative Medicine, 2014, 15(1):1-7. IF:2.02
E-EL-R2466
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E-EL-R0023
E-EL-0041
E-EL-R0023
E-EL-R2466
E-EL-R0329
E-EL-R0022
E-EL-R0015
E-EL-R0019
Insulin
Leptin
Insulin
8-Epi-Prostaglandin F2
Alpha
Insulin
Insulin
Adiponectin
C-Reactive Protein
Interleukin 6
Alpha
Rat
Rat
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IF:2.558
Zhang Y, Imam M U, Ismail M, et al. High fat diet-induced inflammation
and oxidative stress are attenuated by N-acetylneuraminic acid in
rats[J]. Journal of Biomedical Science, 2015, 22(1):1-10. IF:2.763
E-EL-R0016
E-EL-R0566
E-EL-R0084
E-EL-R0009
E-EL-R0566
Interleukin 10
Interleukin 17
Transforming Growth
Factor β1
Interferon Gamma
Interleukin 17
Rat
Rat
Lu X, Mccoy K S, Xu J, et al. Galectin-9 ameliorates respiratory
syncytial virus-induced pulmonary immunopathology through regulating
the balance between Th17 and regulatory T cells[J]. Virus Research,
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E-EL-R0451
E-EL-R0026
E-EL-R0939
E-EL-R0279
E-EL-R0002
E-EL-R0390
E-CL-R0018
Follicle Stimulating Hormone
Gonadotropin-Releasing
Hormone
Luteinizing Hormone
Tartrate-Resistant Acid
Phosphatase 5b
Cross-linked C-Telopeptide of
Type I Collagen
Bone Morphogenetic
Protein 2
Free Thyroxine
Epinephrine
Rat
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2015, 189:50-55. IF:1.562
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brain lactate uptake during hypoglycemia by up regulation of brain
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E-EL-0065
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E-EL-R0884
Estradiol
Bone Alkaline Phosphatase
Signal Transducer And
Activator Of
Transcription 5B
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E-EL-R1401
E-EL-R1402
E-EL-R1235
E-EL-R0772
E-EL-R0624
E-EL-R0618
Amyloid Beta 1-40
Amyloid Beta 1-42
Brain Derived
Neurotrophic Factor
Procollagen I N-Terminal
Peptide
Rat
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Hong Y J, Liang J G, Jiang N Y, et al. Vitamin K3 increased BMD at
1 and 2 months post-surgery and the maximum stress of the middle
femur in the rat[J]. Nutrition Research, 2015, 35(2):155-161. IF:2.472
Liu Y, Zhong G, Zhao Z. Effect of chronic left ventricular unloading
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heart transplantation techniques[J]. Journal of Heart & Lung
Transplantation the Official Publication of the International Society for
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E-EL-R0050
E-EL-R0841
Osteoprotegerin
Receptor Activator of
Nuclear Factor Kappa B
Ligand
Rat
Zheng X, Wu G, Nie Y, et al. Electroacupuncture at the governor
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20
E-EL-R1327
E-EL-R0045
E-EL-R2430
E-EL-R2466
E-EL-R0582
E-EL-M2416
E-EL-M1272
E-EL-M0160
E-EL-R0573
Creatine Kinase MB
Isoenzyme
Collagen Type II Alpha 1
Insulin
Leptin
Fibroblast Growth Factor
Receptor 1
Alanine Transaminase
Aspartate
Aminotransferase
Mucin 2
Rat
Rat
Rat
Mouse
Rat
Hao G, Yu Y, Gu B, et al. Protective effects of berberine against
doxorubicin-induced cardiotoxicity in rats by inhibiting metabolism of
doxorubicin.[J]. Xenobiotica; the fate of foreign compounds in biological
systems, 2015, 45(11):1024-1029. IF:2.199
Yang E, Zheng H, Peng H, et al. Lentivirus-induced knockdown of
LRP1 induces osteoarthritic-like effects and increases susceptibility
to apoptosis in chondrocytes via the nuclear factor-κB pathway[J].
Experimental & Therapeutic Medicine, 2015, 10(1):97-105. IF:1.269
Wu T, Qi X, Liu Y, et al. Dietary supplementation with purified mulberry
,（Morus australis, Poir）anthocyanins suppresses body weight gain in
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S-J L, Jung Y H, Oh S Y, et al. Vibrio vulnificus VvpE inhibits mucin 2
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in intestinal epithelial cells[J]. Cell Death & Disease, 2015, 6(6):e1787.
IF:5.014
E-EL-0065
E-EL-0072
E-EL-R0391
Estradiol
Testosterone
Follicle Stimulating
Hormone
Rat
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Journal of Assisted Reproduction & Genetics, 2015, 32(7):1097-1104.
IF:1.718
E-EL-R0167
E-EL-R0693
E-EL-R0644
E-EL-R0710
E-EL-R0965
E-EL-R0966
E-EL-R0582
E-EL-R1079
E-EL-0022
E-EL-R0633
E-EL-M0049
Endothelin 1
Orexin A
Orexin B
Oxidized Lowdensity
Lipoprotein
Thromboxanes B2
Thromboxanes B3
Leptin
Von Willebrand Factor
Prostacyclin
Monocyte Chemotactic
Protein 1
Alpha
Rat
Rat
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Rat
Mouse
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Guo S J, Cui Y, Huang Z Z, et al. Orexin A-mediated AKT signaling
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reinstatement of morphine-induced conditioned place preference[J].
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procoagulation effects of high-fat diet in rats[J]. Drug Design
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Yida Z, Imam MU, Ismail M, et al. N-Acetylneuraminic acid attenuates
hypercoagulation on high fat diet-induced hyperlipidemic rats. Food &
Nutrition Research. 2015;59:10.3402 IF:3.028,2.162
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IF:3.369
E-EL-R0796 Prostate Specific Antigen Rat
Afriyie D K, Asare G A, Bugyei K, et al. Treatment of benign prostatic
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21
E-EL-M2608
E-EL-M2603
E-EL-M0048
E-EL-M0043
E-EL-M0047
E-EL-M0595
E-EL-M0676
E-EL-M1073
Free cholesterol
Triglyceride
Interferon Gamma
Interleukin 4
Interleukin 17
GremLin 1
High mobility group protein
B1
Soluble
Endothelial
Protein C
Receptor
Mouse
Mouse
Mouse
Mouse
Mouse
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IF:2.599
Siddique I, Curran S P, Ghayur A, et al. Gremlin promotes peritoneal
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with increased solute transport in peritoneal dialysis patients.[J].
American Journal of Pathology, 2014, 184(11):2976-84. IF:4.591
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the Federation of American Societies for Experimental Biology, 2014,
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E-EL-M0048
E-EL-M0026
E-EL-M0049
E-EL-M0043
E-EL-M0046
Interferon Gamma
Interleukin 12
Alpha
Interleukin 4
Interleukin 10
Mouse
Abdel-Hafeez E H, Ahmad A K, Abdelgelil N H, et al.
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E-EL-M0253
E-EL-M1402
E-EL-M2608
E-EL-M0511
E-EL-0072
E-EL-M0372
Caveolin 1
High Density Lipoprotein
Free cholesterol
follicle-stimulating hormone
Testosterone
Beta Crosslaps
Mouse
Mouse
Mouse
Mouse
Xu W, Nan S, Bai W, et al. Caveolin-1 as a biomarker to predict
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Wu T, Tang Q, Yu Z, et al. Inhibitory effects of sweet cherry
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1363. IF:3.836
E-EL-M0049
E-EL-M0048
E-EL-M0037
E-EL-M0044
E-EL-M0046
E-EL-M0047
Alpha
Interferon Gamma
Interleukin 1 Beta
Interleukin 6
Interleukin 10
Interleukin 17
Mouse
Cheng J, Zhang L, Dai W, et al. Ghrelin ameliorates intestinal
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22
E-EL-M0349 Corticosterone Mouse
Li K, Liu F F, He C X, et al. Olfactory Deprivation Hastens Alzheimer-
Like Pathologies in a Human Tau-Overexpressed Mouse Model via
Activation of cdk5[J]. Molecular Neurobiology, 2014, 53(1):1-11.
IF:5.137
E-EL-M1036
S100 Calcium Binding
Protein A12
Mouse
Zhan Y, Zhang F, Shi Y, et al. Potential Effects of Calcium Binding
Protein S100A12 on Severity Evaluation and Curative Effect of Severe
Acute Pancreatitis[J]. Inflammation, 2014, 38(1):290-7. IF:1.973
E-EL-M0166
E-EL-M0693
SAEP041
SAEP021
E-EL-M2614
E-EL-M1215
E-EL-M0018
E-EL-M0267
Free Testosterone
Immunoglobulin G1
Goat Anti-Mouse IgG
FITC-Goat Anti-Mouse
IgG
Insulin
Tumor Protein 53
Growth Regulated
Oncogene Alpha
Chemokine C-X3-C-Motif
Ligand 1
Mouse
Mouse
Mouse
Mouse
Mouse
Hrincius E R, Liedmann S, Finkelstein D, et al. NS1 mediated inhibition
of c-Abl results in acute lung injury and priming for bacterial co-
infections; insights into 1918 H1N1 pandemic?[J]. Journal of Infectious
Diseases, 2014. IF:5.997
Li Y, Wu Y, Luo C, et al. Rewritable magnetic fluorescence-encoded
microspheres: preparation, characterization, and recycling[J]. J.mater.
chem.c, 2015, 3. IF:4.696
Song H, Lai J, Tang Q, et al. Mulberry ethanol extract attenuates
hepatic steatosis and insulin resistance in high-fat diet-fed mice[J].
Nutrition Research, 2016. IF:2.472
Zhang D Y, Shen X Y, Qin R, et al. Effects of subchronic samarium
exposure on the histopathological structure and apoptosis regulation
in mouse testis[J]. Environmental Toxicology & Pharmacology, 2014,
37(2):505-512. IF:2.084
Wang H, Hong L J, Huang J Y, et al. P2RX7 sensitizes Mac-1/ICAM-1-
dependent leukocyte-endothelial adhesion and promotes neurovascular
injury during septic encephalopathy[J]. Cell Research, 2015, 25(6).
IF:12.413
E-EL-M0132 Apolipoprotein B Mouse
Smallwood T L, Gatti D M, Quizon P, et al. High-resolution genetic
mapping in the diversity outbred mouse population identifies apobec1
as a candidate gene for atherosclerosis.[J]. G3 (Bethesda, Md.), 2014,
4(12):2353-2363. IF:2.511
E-EL-M0204
E-EL-M0511
E-CL-M0049
E-EL-M2614
E-EL-M0002
E-EL-M0039
Brain Natriuretic Peptide
follicle-stimulating hormone
Transforming Growth
Factor β1
Insulin
Adiponectin
Leptin
Mouse
Mouse
Mouse
Mouse
Diané A, Nikolic N, Rudecki A P, et al. PACAP is essential for the
adaptive thermogenic response of brown adipose tissue to cold
exposure.[J]. Journal of Endocrinology, 2014, 222(3):327-39. IF:3.718
Fan Y, Liu Y, Xue K, et al. Diet-induced obesity in male C57BL/6 mice
decreases fertility as a consequence of disrupted blood-testis barrier.[J].
Plos One, 2015, 10(4). IF:3.234
Li J, Zhao X, Liu X, et al. Disruption of TIM-4 in dendritic cell
ameliorates hepatic warm IR injury through the induction of regulatory
T cells[J]. Molecular Immunology, 2015, 66(2):117-125. IF:2.973
Chirag A. Patel, Jalaram H. Thakkar, Priyanka Patel, et al. LIPASE
INHIBITORY AND ANTI-OBESITY EFFECT OF THE HYDRO
ALCOHOLIC EXTRACT OF PINUS SYLVESTRIS BARK[J]. 2015,
4(4):1213-1229. IF:0.987
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23
E-EL-P0184
Brain Derived
Neurotrophic
Factor
Porcine
Yue C, Zheng Z, Xi Z, et al. Lactoferrin Promotes Early
Neurodevelopment and Cognition in Postnatal Piglets by Upregulating
the BDNF Signaling Pathway and Polysialylation[J]. Molecular
Neurobiology, 2014, 52(1):256-269. IF:5.137
EBLK0002 Biotin Labeling Mouse
Shao Y, Deng T, Zhang T, et al. FAM19A3, a novel secreted protein,
modulates the microglia/macrophage polarization dynamics and
ameliorates cerebral ischemia[J]. Febs Letters, 2015, 589(4):467-75.
Chen C, Lv Y, Shi Q, et al. Preparation of human tau exon-2- and -10-
specific monoclonal antibodies for the recognition of brain tau proteins
in various mammals.[J]. International Journal of Molecular Medicine,
2015, 140(11):1889-1904. IF:3.169,2.088
E-EL-P0038
Phosphodiesterase 4D,
cAMP Specific
Porcine
Guan W, Liu W, Bao J, et al. Analysis and application of a neutralizing
linear epitope on liable toxin B of enterotoxin Escherichia coli[J].
Applied Microbiology & Biotechnology, 2015, 99(14):5985-5996.
IF:3.337
E-EL-0009 Folic Acid/Vitamin B9
Mitten
Crab
Wei J, Yu N, Tian W, et al. Dietary vitamin B 12, requirement and its
effect on non-specific immunity and disease resistance in juvenile
Chinese mitten crab Eriocheir sinensis[J]. Aquaculture, 2014, 434:179-
183.
Wei J J, Zhang F, Tian W J, et al. Effects of dietary folic acid on growth,
antioxidant capacity, non-specific immune response and disease
resistance of juvenile Chinese mitten crab Eriocheir sinensis, (Milne-
Edwards, 1853)[J]. Aquaculture Nutrition, 2015. IF:1.878,1.395
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