2. ION CHANNELS
• The lipid bilayer of the plasma membrane is Impermeable to anions and
cations.
• Ion channel are complex or single protein that catalyzes the passage of
specific ions through the membrane and establish Concentration gradient
across the membrane.
• Changes in the flux of ions across the plasma membrane are critical
regulatory events in both excitable and non-excitable cells.
3. SODIUM CHANNEL
Sodium channels are integral membrane proteins that form ion channels,
conducting Sodium ions (Na⁺) through a cell's plasma membrane.
TYPES
1. Voltage-gated Na⁺ channels (NavC).
2. Ligand-gated Na⁺ channels or Receptor operated Na⁺ channels (LGNaC).
3. Epithelial Na⁺ channels (ENaCs) .
4. Na⁺-K⁺ -ATPase Pump.
5. Na⁺ dependent transporters (Symport and Antiport systems).
5. VOLTAGE GATED Na⁺
CHANNEL
These channels are responsible for the generation of action potentials that depolarize
the membrane from its resting potential of –70 mV up to a potential of +20 mV
within a few milliseconds.
This channel has several function parts.
One portion of the channel determines its ion selectivity and another portion of the
channel serves as Gate that can open or close.
Incase of Na⁺V channel the gate is controlled by Voltage sensor which responds to
the level of Membrane Potential.
6. WHAT IS MEMBRANE POTENTIAL ?
Membrane potential is the difference in electrical potential between the interior
and exterior of the cell.
Electrical potential exists across the membranes of virtually all cells of the body.
The Resting Membrane Potential of large nerve fibres when not transmitting
nerve signals is about -70 millivolts.
7. The Membrane potential established are used :
1. By Excitable tissues such as nerve and muscle to generate and transmit Electrical
impulses.
2. By non-excitable cells to trigger biochemical and secretory events.
3. By all cells to support a variety of secondary symport and antiport processes.
8. ACTION POTENTIAL
Action Potential is defined “as a rapid rise
and subsequent fall in voltage or membrane
potential across a cellular membrane”.
Stages of Action potential are:
1) Resting stage
2) Depolarization stage
3) Repolarization stage.
4) Hyperpolarization.
9. STRUCTURE OF VOLTAGE GATED SODIUM CHANNEL
NavC are composed of Three subunits.
One pore-forming α subunit, Two regulatory β subunits.
α Subunit: An α subunit forms the core of the channel and has
four repeat domains, labelled I through IV .
Each domain containing six membrane-spanning segments, labeled
S1 through S6.
The highly conserved S4 segment acts as the Channel voltage
sensor and is due to positive amino acids located at every fourth
position.
11. GATING OF Na⁺ CHANNEL
Voltage-gated Na⁺ channels exist in any of three distinct states:
I. DEACTIVATED (CLOSED): During resting membrane potential (RMP) the
channel is blocked on the extracellular side by their activation gates (AG).
II. ACTIVATED (OPENED): After action potential is reached, the activation gates
(AG) open, allowing positively charged Na⁺ ions to flow into the neuron/cell
through the channels.
III. INACTIVATED (CLOSED): Inactivation of the activated channel.
13. DRUGS ACTING ON NaV CHANNEL
ACTIVATORS
ACONITINE: A toxin produced by the Aconitum plant and was previously used as
antipyretic and analgesic.
BATRACHOTOXIN (BTX): Extremely potent cardiotoxic and neurotoxic
steroidal alkaloid found in certain species of frogs (poison dart frog), melyrid
beetles, and birds.
BREVETOXIN: Cyclic polyether compounds produced naturally by a species of
dinoflagellate known as Karenia brevis. Brevetoxins are neurotoxins.
CIGUATOXIN: Produced by Gambierdiscus toxicus, a type of dinoflagellate
Accumulates in skin, head, viscera of big reef fishes. Causes Ciguatera.
14. DRUGS ACTING ON NaV CHANNEL
ACTIVATORS
DELPHININE: Toxic diterpenoid alkaloid found in plants from the Delphinium
and Atragene genera. Has similar effects to aconitine, acting as an allosteric
modulator of NavC. It is mostly cardiotoxic.
GRAYANATOXINS : produced by plants in the Ericaceae family. Prevents
sodium channel inactivation.
VERATRIDINE: It is a steroid-derived alkaloid from plants in the Liliaceae
family that functions as a neurotoxin.
15. Na⁺V CHANNEL BLOCKERS
EXTRACELLULAR
1) Tetrodotoxin(TTX): Derived from puffer fish, toads (Atelopus), blue ringed
octopus and bacteria like Vibrio, Pseudomonas etc.
2) Saxitoxin (STX) : a Neurotoxin naturally produced by certain species of marine
dinoflagellates. Ingestion of saxitoxin by humans, is responsible for the illness
known as Paralytic shellfish poisoning (PSP).
3) Neosaxitoxin (NSTX): included, as other Saxitoxin-analogs, in a broad group of
natural neurotoxic alkaloids, commonly known as the paralytic shellfish toxins
(PSTs).
16. INTRACELLULAR
1) Local anesthetics :
I. Long acting: Bupivacaine, Ropivacaine, Tetracaine.
II. Intermediate acting: Lignocaine, Prilocaine, Cocaine.
III. Short acting : Procaine, Benzocaine.
2) Class I Antiarrhythmic Agents:
I. Class I A: Quinidine, Procainamide, Amiodarone.
II. Class I B: Lignocaine, Phenytoin, Tocainide, Mexiletine.
III. Class I C: Flecainide, Encainide, Propafenone, Moricizine.
3) Anticonvulsants : Phenytoin, Carbamazepine, Valproate,
Lacosamide, Lamotrigine.
18. MECHANISM OF ACTION
Block the NavC and consequently block the nerve conduction by reducing the
permeation of Na⁺ ions during depolarization.
The blocking action of Local anaesthetics is favoured by Depolarization.
Resting membrane is less sensitive to Local anaesthetics.
19. USES
SURFACE ANAESTHESIA
Topical application of an Local Anaesthetics to mucus membrane or skin.
Sites:
Eyes- Tonometry, surgery.
Nose/Ear- painful lesions, polyps.
Mouth/Throat- Painful ulcers, stomatitis.
Pharynx/Larynx- Tonsillectomy, Endotracheal intubation.
Urethra- Dilatation, Catheterization.
20. INFILTRATION ANAESTHESIA
Dilute solution of LA is infiltrated under the skin in the area of operation. Used
for incision, excision, hydrocele, herniorrhaphy etc.
CONDUCTION BLOCK
LA is injected around nerve trunk. Area distal to injection is anaesthetized and
paralyzed.
Field block: Used in herniorrhaphy, appendicectomy, dental procedures.
Nerve block: Lingual block, Ulnar block, Brachial plexus block, Sciatic and
femoral block etc.
21. SPINALANAESTHESIA
LA is injected in the subarachnoid space between L2-3 or L3-4.Nerve roots in the
cauda equina are blocked. Used in prostatectomy, fracture setting, obstetric
procedures etc.
EPIDURAL ANAESTHESIA
Categories: Thoracic, lumber and caudal; depending on the site of injection.
Uses- obstetric purposes and post operative pain relief.
INTRAVENOUS REGIONALANAESTHESIA
Injection of LA into vein of a tourniquet occlude limb. Used for the orthopaedic
procedures in the upper limb.
22. CLASS I ANTI ARRHYMITIC AGENTS
These drugs block the Voltage gated Na⁺ channels same as Local anaesthetics.
Reduce Maximum rate of depolarization (MRD) during Phase 0 of action potential
(AP).
Binds more strongly to the Activated and the Inactivated channels.
Inhibitory property is ‘use dependence’, more frequently the Na⁺ channels are
activated the greater will be the block.
24. SUBDIVISIONS
Class IA Drugs
Quinidine, Procainamide, Disopyramide, Amiodarone.
MECHANISM OF ACTION
Blocks open Na⁺ channels > inactivated channels.
Slow rate of rise of Phase 0 of AP.
Increase the effective refractory period (ERP).
Prolong the action potential duration (APD) or repolarization.
Decrease the slope of phase 4 depolarization in pacemaker cells other than SA node.
27. Class IB Drugs
Lignocaine, Phenytoin, Mexiletine and Tocainide.
MECHANISM OFACTION
Blocks open < Inactivated Na⁺ channels. Have a rapid rate of association and
dissociation.
Shortening of the phase 3 repolarization.
Decrease ERP and APD.
Prolong the diastolic phase 4 depolarization.
Lack effect on Healthy myocardium and conducting tissue but blocks Na⁺ channels
in diseased myocardium.
29. THERAPEUTIC USES
Ventricular tachycardia (VT).
Premature ventricular contractions (PVCs).
Ventricular extra-systoles that result from Acute MI and open heart
surgery.
Not useful for treatment of arrhythmias due to automaticity changes.
30. Class IC Drugs
Flecainide, Encainide, Propafenone, Moricizine.
MECHANISM OFACTION
I. Blocks open Na⁺ channels > Inactivated Na⁺ channels. Dissociates very
slowly.
II. Markedly decrease the rate of phase 0 depolarization in Purkinje and ventricular
myocardial fibers.
III. Reduce automaticity, decrease AV conduction and contractility. Have prominent
effect on normal heart as well.
IV. Retard re-entry of retrograde as well as antegrade impulses.
31. THERAPEUTIC USE
Refractory Premature ventricular contractions and ventricular tachyarrhythmia.
Supraventricular arrhythmias (except moricizine).
Wolf-Parkinson-White (WPW) Syndrome.
32. NAV CHANNEL BLOCKER
ANTICONVULSANTS
• Phenytoin, Carbamazepine, Valproate, Lacosamide, Lamotrigine ,Topiramate.
MECHANISM OFACTION:
• Preferentially block the NavC channels in a ‘Use dependent’ manner. Higher the
frequency of firing, greater is the block.
• Prolong the duration of Inactivation phase. And delay its reversion to resting
phase.
• Inhibition of NavC channels diminish glutamate release from excitatory
glutaminergic neurons.
33. PHENYTOIN
USES
1. Generalized tonic-clonic seizure (GTCS).
2. Partial seizure (Psychomotor type).
3. Status epilepticus.
4. Trigeminal neuralgia (2nd choice).
5. Ventricular arrhythmias due to digitalis toxicity.
6. To enhance wound healing.
34. ADVERSE EFFECTS
Over dose toxicity : CNS depression, lethargy.
Chronic toxicity:
I. Gingival hyperplasia & coarsening of facial features.
II. Megaloblastic anaemia, Vitamin D, K deficiency.
III. Hirsutism (in females) & acne.
Congenital malformations- cleft lip & palate, CHD.
Hypersensitivity.
Contraindications: Petit mal (absence seizure).
Myoclonic seizure.
36. ADVERSE EFFECTS
Dose related- Dizziness, vertigo, ataxia, diplopia.
Idiosyncrasy- Allergy, aplastic anaemia, hepatitis, SLE, leucopenia.
Water retention and hyponatraemia.
Teratogenicity- Finger nail hypoplasia, craniofacial defects.
37. VALPROATE
Broad spectrum anticonvulsant.
MECHANISM OF ACTION
• Increase GABA activity. Activates glutamic acid decarboxylase (GAD) & inhibits
GABA transaminase.
• Decrease glutamate synthesis and release in brain.
• Blocks T type Ca²⁺channels.
USES
I. Absence seizure.
II. GTCSs.
III. Myoclonic seizures.
IV. Atonic seizure.
38. ADVERSE EFFECTS
DOSE RELATED
I. Weight gain
II. Reversible alopecia
III. Tremors.
IDIOSYNCRATIC TOXICITY
Hepatitis, Pancreatitis, Thrombocytopenia and allergy.
TERTATOGENICITY
Neural tube defects.
40. LIGAND - GATED Na⁺ CHANNEL
Activated by Binding of a Ligand instead of a change in membrane potential.
Members of a superfamily of ligand-gated ion channels (LGIC).They don’t
possess the exquisite ion selectivity.
TYPES
1. Nicotinic acetylcholine receptor (nAChR).
2. N-methyl-D-aspartate (NMDA) receptor.
3. α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor.
42. STRUCTURE: NICOTINIC ACETYLCHOLINE
RECEPTOR
Pentameric Channel.
Muscle-Type nAChR consists of 4 different
subunits (2α, β, δ, ε/γ).
Neuronal-Type nAChR consists of 2
different subunits (2α, 3β).
Each α subunits has an identical negatively
charged binding site for ACh.
43. DRUGS ACTING ON NAChR
1) AGONISTS: Acetylcholine, Nicotine.
2) NEUROMUSCULAR BLOCKING AGENTS :
A. (I) Depolarising Nm blockers : Succinylcholine, Decamethonium.
B. (II) Nondepolarizing/Competitive blockers:
I. Long acting: d-Tubocurarine, Pancuronium, Doxacurium, Pipecuronium.
II. Intermediate acting: Vecuronium, Atracurium, Rocuronium, Rapacuronium.
III. Short acting: Mivacurium.
3) GANGLION BLOCKER: Trimethaphan.
Mecamylamine.
44. ACETYLCHOLINE
This is the endogenous ligand for nAChRs.
ACh does not have any therapeutic uses due to it’s ultra short duration of action.
NICOTINE
It is the principal alkaloid in tobacco.
Ganglionic stimulant. Large doses cause persistent depolarization and ganglionic
blocked.
THERAPEUTIC USE
I. Short term nicotine replacement in tobacco abstinence.
II. Treatment of tobacco cessation.
45. NEUROMUSCULAR BLOCKING AGENTS
(DEPOLARISING BLOCKERS)
SUCCINYLCHOLINE (SCh)
MECHANISM OFACTION:
Structural analogue of Ach hydrolysed by pseudocholinesterase.
Stimulates NM receptor and depolarizes the membrane. Initial fasciculation
occurs.
Prolong Depolarisation renders the membrane refractory to other impulses and
muscle relaxation occurs.
46. USES
1) Electro-convulsive therapy.
2) Endotracheal intubation.
3) Esophagoscopy.
4) Laryngoscopy.
5) Bronchoscopy.
6) Reduction of fractures and dislocations.
7) To treat laryngospasm.
47. ADVERSE EFFECTS & CONTRAINDICATIONS
1. Hyperkalaemia (CI in nerve and muscle diseases).
2. Prolonged apnoea in Atypical Pseudocholinesterase.
3. Increases IOP (CI in Glaucoma).
4. Increases ICT (CI in head injury).
5. Increases BP (Sympathetic ganglia stimulation).
6. Malignant hyperthermia.
7. Post operative muscle pain and soreness (due to initial fasciculation).
48. COMPETATIVE NEUROMUSCULAR BLOCKERS
CLASSIFICATION:
ISOQUINOLONE DERIVATIVES:
I. Long acting: d-Tubocurarine, Metocurine, Doxacurium.
II. Intermediate acting: Atracurium, Cisatracurium.
III. Short acting: Mivacurium.
STEROIDAL DERIVATIVES:
I. Long acting: Pancuronium, Pipecuronium.
II. Intermediate acting: Vecuronium, Rocuronium.
III. Short acting: Rapacuronium.
49. MECHANISM OF ACTION
These have an affinity for the Nm cholinergic
receptors at the motor end plate, but have no
intrinsic activity over them.
Bulk of the molecule block the Nm receptor and
prevent its binding to Ach.
As a result necessary conformational change
needed for sodium channel opening is prevented.
50. ACTIONS
Skeletal muscle- Flaccid paralysis without initial fasciculation.
Autonomic ganglia blocked.
Histamine release by d-Tubocurarine.
Significant fall in BP.
USES
Adjuvant to GA.
Assisted ventilation.
Status epilepticus.
Severe tetanus.
51. ADVERSE EFFECTS
I. Respiratory paralysis.
II. Flushing (d-TC).
III. Fall in BP.
IV. Precipitation of asthma.
V. Life threatening bronchospasm by Rapacuronium
52. GANGLION BLOCKERS
Antagonist at NN receptors
Compete with ACh for the receptor site on the autonomic Ganglia thereby
blocking the transmission of impulse from preganglionic to postganglionic neuron.
NN RECEPTOR ANTAGONISTS:
A. Persistent Depolarising blockers : Nicotine (high doses) , Anticholinesterase
(High doses)
B. Non depolarising Blockers (Competitive blockers ): Trimethaphan,
Mecamylamine.
53. NN RECEPTOR ANTAGONISTS
TRIMETHAPTHAN
Ultra short acting ganglion blocker given by IV infusion.
USES:
I. In Hypertensive Emergencies and produce Controlled hypotension.
II. Hypertensive emergencies due to aortic aneurysm.
III. Management of autonomic hyper-reflexia.
54. N-METHYL-D-ASPARTATE (NMDA) RECEPTOR
Widely distributed in the CNS. Stimulated by Glutamate.
Ligand gated ion channel receptor
High permeability to Na⁺ and Ca²⁺ ions.
STRUCTURE
Heterotetramer consisting two NR-1 And two NR-2 subunits.
Six pharmacologically distinct modulatory or binding sites present:
1) GLU/NMDA binding site.
2) Modulatory site that binds to Glycine.
55. NMDA RECEPTOR SITES
3) Polyamines Regulatory site.
4) PCP site: Binds to Phencyclidine and antagonists
like Ketamine, Memantine, Amantidine.
5) Voltage dependent Mg²⁺ binding site: Inhibitory.
6) Voltage dependent Zn²⁺ binding site: Inhibitory.
56. DRUGS ACTING ON NMDA RECEPTOR
NMDA RECEPTOR BLOCKERS
1. Antiepileptic agents- Felbamate
2. General anaesthetic- Ketamine
3. Anti-parkinsonian drug- Amantadine
4. Drugs against Alzheimer’s disease- Memantine
57. DRUGS ACTING ON NMDA RECEPTOR
FELBAMATE
Blocks glycine binding site.
Also blocks NaVC (minor).
USES
I. Lennox-Gastaut Syndrome
II. Atonic seizures.
III. Atypical absence seizures.
IV. Partial seizure, GTCS.
ADVERSE EFFECTS
I. Unpredictable aplastic anaemia,
II. Hepatotoxicity.
58. KETAMINE
Produces Dissociative Anaesthesia.
Blocks NMDAR at in cortex and limbic system.
Very strong analgesic agent.
Acts by blocking action of Glutamate at NMDA Receptor.
USES
I. IV anaesthesia of choice in/for patient with shock (increases BP, ICT, IOP).
II. Emergency surgery with full stomach (does not depress laryngeal & pharyngeal
reflexes).
III. Patient with bronchial asthma.
IV. Induction in children.
59. AMPA and KAINATE Receptor
α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (AMPA) and
Kainate receptors are also known as Non-NMDA receptor.
Widely distributed in the CNS. Mediate fast excitatory synaptic transmission
associated with Na⁺ & Ca²⁺influx.
Stimulated by Glutamate.
STRUCTURE
AMPA Receptors are pentamers consisting of GLUR₁₋₄ subunits.
Kainate receptors are also pentamers consisting of GLUR₅₋₇ subunits and KA₁₋₂
subunits.
63. Na⁺-K⁺-ATPase PUMP
Na⁺-K⁺-ATPase enzyme actively Transports Na⁺ and K⁺ across all mammalian
cell membrane.
Virtually presents in all mammalian cells.
Its function is essential for all mammalian cell physiology.
Belongs to P-type ATPase family of cation pump.
64. STRUCTURE
It is a membrane protein.
Consists of Large catalytic α subunit with ten trans-membrane segments.
Glycosylated β subunit with single trans-membrane segment (Stabilization).
Regulatory FXYD proteins (γ subunit).
Isoforms of α, β & FXYD proteins: α 1-4, β 1-3, FXYD 1-7.
α1 is the housekeeping form. α2 is present in heart and other muscles.
66. Covalent phosphorylation of an active site
aspartate residue by ATP.
Conformational change from E1 to E2 form
coupled with cation movement.
Moves three Na⁺ out and two K⁺ into the cell
against concentration gradient.
Hydrolysis of phosphoenzyme brings back
E2-E1 conformational change.
67. FUNCTIONS
Maintains Resting membrane potential (RMP).
Transport of ions, glucose and amino acids (by use of Na⁺ gradient).
Controlling cell volume.
Functioning as signal transducer.
Controlling neuron activity states.
68. Na⁺-K⁺-ATPase Pump
MODULATOR
ENDOGENOUS :
I. cAMP- Upregulation
II. Aldosterone
INHIBITOR of Na⁺ K⁺ ATPase PUMP :
CARDIAC GLYCOSIDES
I. Digoxin
II. Digitoxin
III. Ouabain
69. SODIUM CHANNEL MODULATOR
ALDOSTERONE
Most important mineralocorticoid in humans. Secreted from Zona
glomerulosa of adrenal glands.
MECHANISM OF ACTION
I. Direct stimulation of Na⁺/H⁺ exchanger in the apical membrane.
II. Acts by binding to the cytosolic Mineralocorticoid receptor and inducing
synthesis of Aldosterone induced protein (AIP).
III. Increases number of Na⁺/K⁺ ATPase molecules ( Na⁺ pumps) in the
basolateral membrane.
71. CARDIAC GLYCOSIDES
The cardiac glycosides are often called digitalis or digitalis glycosides.
They are a group of chemically similar compounds that increase the contractility
of the heart muscle and therefore are used in treating heart failure.
The digitalis glycosides have a low therapeutic index.
The most widely used cardiac glycosides is digoxin.
72. DIGOXIN
PHARMACOLOGICALACTIONS ON HEART
Positive inotropic action
Bradycardia
EFFECTS ON ACTION POTENTIAL
Resting Membrane potential is decreased.
Rate of phase 0 depolarization is reduced (marked in AV node).
Slope of phase 4 depolarization is increased (in PF).
77. EPITHELIAL SODIUM CHANNEL
It is also known as Sodium Channel non-neuronal 1(SCNN 1)
Is a membrane-bound ion-channel that is permeable for Li⁺-ions, protons, and
especially Na⁺-ions.
It is a constitutively active ion-channel.
Location:
Kidney, colon, lung & sweat gland.
Also in pancreas, testes & ovaries.
78. STRUCTURE
ENaC consists of three different subunits: α, β, γ.
Each of the subunits consists of two
transmembrane helices and an extracellular loop.
The amino- and carboxyterminal of all
polypeptides are located in the cytosol.
In pancreas, testes and ovary, δ-subunit is
present in place of α-subunit.
79. FUNCTION
1. It is involved in the transepithelial Na⁺-ion transport, which it accomplishes
together with the Na⁺-K⁺-ATPase pump.
2. Na⁺- and K⁺- ion homeostasis of blood and epithelia and extra-epithelial fluids
by reabsorption of Na⁺-ions.
3. Plays an important role in salt taste perception.
4. Involves in pathophysiology of cystic fibrosis.
82. AMILORIDE/TRIAMTERENE
• They are Potassium sparing diuretics.
MECHANISM OFACTION
• Inhibits the amiloride-sensitive epithelial Na⁺ channels present on the luminal
membrane of late distal tubule (DT) and collecting duct (CD).
• Amiloride at high dosage inhibit Na⁺ reabsorption at PT.
PHARMACOLOGICALACTIONS
I. Weak diuretic.
II. Indirectly inhibit K⁺ secretion from DCT.
III. Also inhibit H⁺ secretion from the CD.
83. USES
I. Used with high ceiling diuretics or thiazides to prevent hypokalaemia.
II. Cystic fibrosis.
ADVERSE EFFECTS
I. Common: Risk of hyperkalaemia
II. Acidosis
III. Triamterene: Impaired glucose tolerance. Photosensitivity.
IV. Amiloride: Nausea, diarrhoea.
85. HIGH CEILING DIURETICS
(Na⁺-K⁺-2Cl CO-TRANSPORTER BLOCKER)
Furosemide, Torsemide, Bumetanide.
MECHANISM OF ACTION
Inhibit Na⁺-K⁺-2Cl⁻ symport from the luminal side.
Increases urinary excretion of Mg²⁺ ,Ca²⁺ in addition to Na⁺
SITE OF ACTION
I. Thick ascending loop of Henle.
II. Also a weak carbonic anhydrase inhibitor.
90. USES
1) Mild to moderate cases of oedema.
2) Oedema of cardiac origins respond better.
3) Hypertension.
4) Diabetes insipidus.
5) Hypercalciuria.
91. Complications of High Ceiling and Thiazide Type
Diuretics
Hypokalaemia, Hypocalcaemia with high ceiling diuretics, Hypomagnesaemia.
Hyperuricaemia-more with thiazides.
Hyperglycaemia & hyperlipidaemia.
Hearing loss.
Allergic manifestations.
Dilutional hyponatraemia.
Thiazides may aggravate renal insufficiency. Should be avoided in Pre-eclampsia.
May precipitate mental disturbances and hepatic coma in cirrhotic patients on brisk
diuresis.
92. REFERENCES
Goodman and Gilman’s the pharmacological basis of therapeutics.
Principles of pharmacology, 3rd edition, HL Sharma, KK Sharma.
Essentials of medical pharmacology, 7th edition. KD Tripathy.
Guyton and hall textbook of Medical physiology,12 edition. John E.Hall
Various internet sources.