3. OVERVIEW
Paired Retroperitoneal Organ
Loacation : Between T12- L3
Right kidney is slightly more caudal
Left kidney is usually somewhat longer
and narrower
Weight : 125 to 170 g in adult males &
115 to 155 g in adult females
Measurements : 11 to 12 cm long, 5.0
to 7.5 cm wide, and 2.5 to 3.0 cm thick.
Less than 9cm- small kidney
5. GROSS ANATOMY
A granular outer region - the CORTEX
1 cm in thickness
Cortex extends downward between the
individual pyramids to form the columns of
Bertin.
MEDULLARY RAYS :cortical collecting
ducts, the straight segments of the proximal
tubules, and the cortical thick ascending
limbs (TALs) of loop of Henle, aligned
together
6. A striated inner region, the MEDULLA -composed of renal pyramids -
conical tissue masses with the base of each pyramid at the
corticomedullary boundary and the apex extending toward the renal
pelvis, forming a papilla.
Each pyramid contains the descending and ascending limbs of the
renal tubules and the collecting tubules
On the tip of each papilla is the area cribrosa, where the distal ends of
collecting ducts (ducts of Bellini) open into the renal pelvis.
7. Renal pelvis - expanded portion of the
upper urinary tract
Two- three extensions of the renal pelvis,
the major calyces which further divides
into several minor calyces. These parts
receive the urine discharged at the area
cribrosa of each renal pyramid.
The ureter - originate from the distal
renal pelvis at the ureteropelvic junction.
Ureters - 28 to 34 cm long and a mean
diameter of 1.8 mm.(max 3mm.)
SMC & interstitial cells- pacemaker
function
14. NEPHRON
THE NEPHRON IS THE FUNCTIONAL UNIT OF
THE KIDNEY
Nephron = Renal corpuscle ( glomerulus and
Bowman’s capsule) + associated renal tubules upto
Distal connecting tubule.
900,000 to 1 million per kidney.
origin of the nephron is the metanephric blastema.
17. TYPE OF
NEPHRONS
CORTICAL NEPHRONS JUXTA-MEDULAARY
NEPHRONS
85% 15%
Shorter loops of Henle
that bend within the inner
stripe of the outer
medulla.
long loops of Henle that
extend into the inner
medulla
No or very short
ascending thin limbs
Long descending and
ascending
thin limb segments
Efferent Arteriole - forms
peritubular capillaries
Efferent Arteriole - forms
vasa recta
Glomerulus - Small Glomerulus - Large
18.
19. Cortex - arcuate vessels
OMo - terminal portion of
PSTs, TALs, and
collecting ducts.
Omi - TALs, descending
thin limbs, and collecting
ducts, but no proximal
tubules
IM - descending and
ascending thin limbs and
collecting ducts, but no
TALs
21. LIGHT MICROSCOPY Glomerulus - composed of a capillary
network lined by a thin layer of
endothelial cells, a central region of
mesangial cells with surrounding matrix
material, and the visceral epithelial
cells (podocytes) overlying the
capillaries.
“Renal Corpuscle” - glomerulus and
Bowman’s capsule.
The average diameter of a glomerulus
is approximately 200 μm.
25. CAPILLARY
ENDOTHELIUM
Fenestrations- 70 to 100 nm in diameter
Glomerular endothelium is covered by a glycocalyx layer,
proteoglycans (syndecan and glypican) with
glycosaminoglycans (GAGs), secreted glycoproteins
(perlecan and versican), and secreted gags (hyaluronan),
which provide a negative charge.
Endogenous albumin is largely confined to the glomerular
capillary lumen and does not pass through the
endothelium.
Injection of hyaluronidase, a hyaluronan degrading
enzyme, in mice led to disruption of the glomerular
endothelial glycocalyx and leakage of albumin across the
endothelium.
26. GLOMERULAR
BASEMENT
MEMBRANE
3 layers
a central dense layer - the lamina densa
two thinner, more electron-lucent layers, the lamina
rara externa and the lamina rara interna
Thickness - in men -373 nm and in women - 326 nm
Primarily of type IV collagen, laminin, nidogen
(entactin), and heparan sulfate proteoglycans
The α3α4α5(IV)-α3α4α5(IV) network predominates n
the GBM, whereas the α1α2α1(IV)-α5α6α5(IV)
network is in Bowman’s capsule.
Both a size-selective and a charge-selective barrier
27. PODOCYTES
Podocytes (visceral epithelial cells) - the largest cells in the glomerulus. They
do not replicate
The cell bodies give rise to long cytoplasmic primary processes that branch
into secondary and tertiary processes, surround the capillaries, and finally
divide into foot processes.
The gap between adjacent foot processes is bridged by a thin structure called
the “filtration slit diaphragm”.
In glomerular diseases associated with proteinuria, the podocyte cytoskeleton
is disrupted, slit diaphragms are lost, and the interdigitating foot processes are
replaced by broad regions of podocyte processes covering the GBM. This is
called “foot process effacement”
28. SLIT DIAPHARGM
Bridges the 30–40 nm space - called the “filtration slit” between adjacent foot processes
Rodewald and Karnovsky proposed a porous zipper-like model for the SD.
Enhanced SEM revealed variable-shape pores in the center of the SD and no central filament. This
finding is more consistent with the SD as a heteroporous structure rather than the zipper-like model
role of the SD - as a size-selective element in the filtration barrier.
30. MESANGIUM
Mesangial Cells With Surrounding Matrix Provide
Scaffold For The Surrounding Glomerular Capillaries.
Possess An Extensive Array Of Microfilaments
Containing Actin, Myosin, And Α-actinin - Functional
Properties Of Smooth Muscle Cells
Mesangial Cell Has Contractile Properties And Is
Thought To Play A Role In The Regulation Of
Glomerular Filtration
Local Generation Of Autacoids, Such As
Prostaglandin E2, By The Mesangial Cell
Exhibit Phagocytic Properties
Generation And Metabolism Of The Extracellular
Mesangial Matrix
32. Represents a major structural component of the renin–angiotensin system and
contributes to the regulation of glomerular arteriolar resistance and glomerular
filtration.
Vascular components - terminal portion of the afferent arteriole, the initial portion
of the efferent arteriole, and the extraglomerular mesangium.
Tubular component - the macula densa, located in the terminal portion of the TAL
that lies between the afferent and efferent arterioles
Extraglomerular mesangium, also called the polar cushion- polkissen or the lacis
Specialized cell types of the JGA include
1- juxtaglomerular granular cells
2-the agranular extraglomerular mesangial cells
3- the epithelial cells that make up the macula densa.
33. PROXIMAL
TUBULE
1- Proximal Convoluted Tubule (PCT - Pars
Convoluta), which originates at the glomerular
urinary pole , located in cortex
2- Proximal Straight Tubule (PST - Pars Recta)-
distal to the PCT and located in the medullary ray in
the cortex
Length- 14 mm
S1- initial 2/3 of PCT
S2 – reminder 1/3 of PCT and initial of PST
S3 – reminder of PST
Major function – reabsorption
34. S1 SEGMENT OF PROXIMAL
TUBULE
A tall brush border
A prominent endocytic–lysosomal
apparatus
Extensive invaginations of the
basolateral plasma membrane
Numerous long mitochondria
aligned among the basolateral
plasma membrane infoldings.
35. S2 SEGMENT OF A
PROXIMAL TUBULE
The brush border is shorter than in
the S1 segment.
Mitochondria are numerous and
generally aligned with the
basolateral plasma membrane
infoldings.
Numerous small lateral processes at
the base of the cell
36. S3 SEGMENT OF A
PROXIMAL TUBULE
The brush border is tall
The endocytic–lysosomal apparatus
is less prominent than in the S1 and
S2
Mitochondria are scattered randomly
throughout the cytoplasm
37. THIN LIMBS OF HENLE
Type I Epithelium – descending thin limb of short-looped
nephrons, smooth
Type II Epithelium- long-looped nephrons contains in the outer
medulla, extensive lateral and basal interdigitations, a “leaky”
epithelium, mitochondria, are more prominent
Type III Epithelium- inner medulla, thinner and simpler in
structure
Type IV Epithelium- generally low and flat, relatively few
organelles
Thin descending limb- water channel, aquaporin-1 present-
highly permeable to water
38. TYPE II EPITHELIUM
OF THE THIN LIMB
OF LOOP OF HENLE
taller and has more
organelles, prominent
apical plasma membrane
microprojections, and
complex basolateral
plasma membrane
infoldings.
39. TYPE III
EPITHELIUM
• prominent apical plasma
membrane
microprojections. It is a
very low, flat epithelium
with relatively few
basolateral plasma
membrane infoldings
compared with type II
epithelium
40. DISTAL
TUBULE
Standard nomenculture- Distal Tubule- TAL of loop of henle (Pars
Recta) + the Distal Convoluted Tubule -DCT (Pars Convoluta)
In Micropuncture Studies- the “early” or “Bright” distal tubule - DCT
plus a short segment of TAL
“Late” or “granular” distal tubule - Connecting Tubule and the initial
portion of the collecting duct in the cortical labyrinth, the ICT.
Whole DCT - NCC present
DCT- Na+–Ca2+ exchanger (NCX1) and vitamin D–dependent
Calcium-Binding protein, Calbindin-D28k.
Late DCT - ENAC and TRPV5 present
DCT - highest Na+-K+-ATPase activity
41. CORTICAL THICK ASCENDING
LIMB (TAL)
Numerous Short Apical
Microprojections
Deep, Complex Invaginations Of The
Basal Plasma Membrane Extend Into
The Apical Region Of The Cell And
Enclose Elongated Mitochondrial
Profiles
Few Organelles And Little Cytoplasm
42. DISTAL CONVOLUTED TUBULE
(DCT)
DCT Cells Is Similar To Thick
Ascending Limb (TAL) Cells
DCT Cells Are Considerably Taller,
With
Numerous Basal Plasma Membrane
Infoldings And Mitochondria Interposed
Between The Nucleus And The
Basement Membrane.
43. CONNECTING
SEGEMNT
1- CNT Cell- Which Occurs Only In This
Segment, Is Tall With An Apically Located
Nucleus, More Cytoplasm And Organelles.
2-Intercalated Cells- Which Account For
Approximately 40% Of The Cells.
3 Subtypes Of Intercalated Cells
1- Type A
2 - Type B
3- Non-a Non-b
44. CNTs Of Superficial
Nephrons Continue
Directly Into ICTs,
Whereas CNTs From
Midcortical And
Juxtamedullary
Nephrons Join To Form
Arcades That Ascend In
The Cortex And Continue
Into ICTs.
45. COLLECTING
DUCT
1- ICT - initial collecting tubule
2- CCD - cortical collecting duct
3- OMCD - outer medullary collecting duct-
OMCDo & OMCDi
4- IMCD - inner medullary collecting duct- IMCDt
& IMCDi
In the ICT, CCD, OMCD, and IMCD, there are
two major cell types:
1- Principal Cells
2- Intercalated Cells
46. INTERSTITIUM
Compartments - the peritubular interstitium and the periarterial connective tissue
Peritubular interstitium - interstitial cells, extracellular matrix and interstitial fluid interposed
between the basement membranes of the renal tubules and peritubular capillaries
Periarterial interstitium - a layer of connective tissue and interstitial cells surrounding the
arteries in the renal cortex [can produce renin]
Fibroblasts, also known as “stellate” or “sustentacular cells,” are the most abundant in the
peritubular interstitium.
In inflammation-myofibroblast. In medulla-numerous lipid inclusions[COX2+, PGE2 synthesis]
Pericytes are contractile cells intimately associated with the capillaries in both the renal
cortex(5NT+) and medulla
Cells of the immune system - the most common are dendritic cells (MHC 2 +)
47. LYMPHATICS
capsular, subcapsular and
intrarenal components
Right kidney lymphatics drain into
the Paracaval, Precaval,
Interaortocaval, and Retrocaval
nodes,
Left kidney lymphatics drain into the
Preaortic, Paraaortic, and
Retroaortic nodes.
48. INNERVATION
• Sympathetic- T6 to L2
• The renal nerves enter the kidney at the hilum and
run with the renal arteries into the kidney in
interlobar, arcuate, and interlobular arteries and in
afferent and efferent arterioles.
• Renin secretion is modulated by renal sympathetic
nerve activity.
Editor's Notes
. It is a dense, homogeneous tissue and consists of renal corpuscles and convoluted portions of renal tubules
Each pyramid contains the descending and ascending limbs of the renal tubules and the collecting tubules
transitional epithelium or urothelium, composed of multiple cell layers, lines the pelvis and ureter
Ureter maximum of 3 mm considered normal
transmits, from anterior to posterior, the renal vein, the anterior branch of the renal artery, the ureter, and the posterior branch of the renal artery
left renal vein passes anterior to the aorta and posterior to the superior mesenteric artery as it approaches the inferior vena cava.
the term renal or Malpighian corpuscle
Collecting duct system is not part of nephron as it has different origin- ureteric bud
loop of Henle contains
the straight portion of the proximal tubule (pars recta),
descending and ascending thin limb segments, and the
straight portion of the distal tubule (TAL, or pars recta)
glomerulus, which is composed
of a capillary network lined by a thin layer of endothelial
cells, a central region of mesangial cells with surrounding
matrix material, and the visceral epithelial cells (podocytes)
overlying the capillaries
“renal corpuscle” is strictly the correct terminology to refer
to the glomerulus and Bowman’s capsule
The average diameter of a glomerulus is approximately
200 μm
70 to 100 nm in
diameter in human, glomerular endothelium is covered by a
glycocalyx layer,
Classic ultrastructural studies demonstrated that endogenous
albumin is largely confined to the glomerular capillary
lumen and does not pass through the endothelium
Note the relationship among the three layers of the glomerular
basement membrane and the presence of the pedicels (P) embedded
in the lamina rara externa (arrowhead). The filtration slit diaphragm
with the central dense spot (thin arrow) is especially evident between
the individual pedicels. The fenestrated endothelial lining of the capillary
loop is shown below the basement membrane.
Type IV collagen consists of six chains, α1(IV) through
α6(IV). Three α(ΙV) chains self-associate intracellularly to
form triple helical molecules called protomers. Three types
of promoters are formed: α1α2α1, α3α4α5, and α5α6α5.
The cell bodies give rise to long
cytoplasmic primary processes that branch into secondary
and tertiary processes, surround the capillaries, and finally
divide into foot processes.