1. Christine Roos Montague
186 Buck Rd Phone: 607-379-1151
Lansing, NY 14882 Email: cmontague70@gmail.com
EDUCATION
University of Virginia, Charlottesville, VA
Ph.D. in Biomedical Engineering, 1995, with high distinction
Chronic inhaled nitric oxide: effects on pulmonary vascular endothelial function and pathology in
hypoxic and normoxic rats.
University of Iowa, Iowa City, IA
M.S. in Biomedical Engineering, 1990
University of Virginia, Charlottesville, VA
B.S. in Mechanical Engineering, 1986
PROFESSIONAL EXPERIENCE
Research Scientist, Agave Biosystems, Ithaca, NY, May 2010-Feb 2015.
 Characterized for a published manuscript the genomic and proteomic responses to two small compounds
identified in high-throughput screening as improving mitochondrial function.
 Optimized high-throughput assays for the identification of agonists or antagonists of the αKlotho and βKlotho-
dependent endocrine Fibroblast Growth Factors.
 Engineered and freeze-dried bacterial and yeast sensors containing toxin-responsive reporter constructs.
 Managed two projects. Communicated the results through periodic reports and presentations.
 Wrote multiple grant proposals including human and animal subjects testing sections: an isothermal
amplification technique to simultaneously detect all five hepatitis viruses in a point-of-care device, a multiplex
assay for arthritis biomarkers, and an amplification device to detect Salmonella in barn waste.
Research Associate, Cornell University School of Engineering, Department of Biomedical
Engineering, Ithaca, NY, July 2008-May 2010, Cynthia Reinhart-King, PI.
 Studied endothelial dysfunction caused by matrix stiffening.
Postdoctoral Associate, Cornell University School of Veterinary Medicine, Clinical Sciences, Ithaca,
NY, July 2006-2008, Lisa A. Fortier, PI.
 Examined the effects of fibronectin fragments on cartilage cells.
Research Associate, Ohio State University School of Medicine, Heart and Lung Research Institute,
Columbus, OH, 1997-2006, Pascal J. Goldschmidt-Clermont and Clay B. Marsh, PIs.
 Characterized estrogen receptor activation in vascular cells and its role in heart disease manifestation in
women.
Postdoctoral Fellow, Johns Hopkins University School of Medicine, Pulmonary and Critical Care
Medicine, Baltimore, MD, 1995-1997, Nicholas A. Flavahan and Pascal J. Goldschmidt-Clermont, PIs.
 Began studies of hormonal and vascular interactions in Cardiology and Pulmonary divisions.
Graduate Research Fellow, University of Virginia Department of Anesthesiology, Charlottesville, VA,
1991-1995, George F. Rich, PI.
 Characterized the responses to inhaled nitric oxide in pulmonary vasculature of rats exposed to chronic
hypoxia.
Teaching Assistant, University of Virginia Department of Mechanical Engineering, Charlottesville, VA,
1991, Thermodynamics.
 Ran tutorials and office hours for thermodynamics.

2. Clinical Research Assistant, Diabetes Research Institute, Norfolk, VA, summer 1990, Aaron I. Vinik.
 Performed EKG and neuropathy testing on clinic patients, introduced and developed fractal analysis of EKG
signal to determine extent of illness.
Mechanical Engineer, Norfolk Naval Shipyard, Portsmouth, VA, 1986-1989.
 Designed installations for upgrades in steam and water systems in nuclear cruisers.
HONORS
1990 The Hand Foundation Graduate Fellowship Award
1992 Three-year graduate fellowship from the Department of Anesthesiology, University of
Virginia School of Medicine
1992 Tau Beta Pi, Engineering Honor Society
1995 Graduate of High Distinction, University of Virginia
1996-1998 NRSA Postdoctoral Fellowship, NHLBI, National Institute of Health (Hormonal
Regulation of Dysfunctional Endothelial Cells)
1991-2001 AHA Ohio Affiliate Postdoctoral Fellowship Award (Enhanced Binding to Fibrinogen of
Glycoprotein IIb-IIIa Receptors with the PlA2 Polymorphism)
RESEARCH SKILLS
Cell biology:
 Primary cell culture, fluorescent microscopy, and transfection.
 Flow cytometry for immunofluorescence, apoptosis assays and BrdU incorporation.
Molecular biology:
 Lentiviral plasmid design, cloning, virus production and transduction.
 Quantitative PCR and design of Taqman primer/probe sets.
 Protein, RNA and DNA purification, blotting and detection. Endotoxin testing.
 Activation domain or immunocomplex precipitation.
 siRNA design for oligo or plasmid transfection or Lentiviral transduction.
 Bioinformatics: analysis of microarray data, eukaryotic and prokaryotic genomes
Assay Development:
 High-throughput experimental design, automation.
 Reporter construct cloning, transformation and analysis.
 PCR and isothermal amplification assay design.
 ELISA development.
 Statistical analysis.
Other Skills:
 Project management and communication with collaborators and sponsors.
 Publication and grant proposal writing, including human and animal research protocols.
 Training, laboratory organization, budgeting, purchasing and scheduling.
PROFESSIONAL MEMBERSHIPS
American Society for Cell Biology
American Heart Association
PUBLICATIONS
1. Montague CR, Fitzmaurice A, Hover BM, Salazar NA, Fey JP. Screen for Small Molecules Increasing the
Mitochondrial Membrane Potential. 2014. J Biomol Screen 19(3):387-98.
2. Huynh J, Nishimura N, Rana K, Peloquin JM, Califano JP, Montague CR, Schaffer CB, King MR, and
Reinhart-King CA. Age-Related Intimal Stiffening Promotes Atherosclerosis by Enhancing Endothelial
Permeability and Leukocyte Transmigration. 2011. Science Translational Medicine 3:112ra122.
3. Ezzie ME, Piper MG, Montague C, Newland CA, Opalek JM, Baran C, Ali N, Brigstock D, Lawler J, Marsh
CB. Thrombospondin-1 Deficient Mice Are Not Protected from Bleomycin-Induced Pulmonary Fibrosis.
2011. American Journal of Respiratory Cell and Molecular Biology 44:556-61.

3. 4. Montague CR, Hunter MG, Gavrilin M, Goldschmidt-Clermont PJ, Marsh CB. Activation of estrogen
receptor-alpha reduces aortic smooth muscle differentiation. 2006. Circulation Research 99:477-484.
Prompted an editorial (Pepine CJ, Nichols WW. Estrogen and Different Aspects of Vascular Disease in
Women and Men. Circulation Research 99:459-461)
5. Lichtenberger FJ, Montague CR, Hunter M, Frambach GE, Marsh CB. NAC and DTT promote TGF-1
monomer formation: demonstration of competitive binding. 2006. Journal of Inflammation; 3:7.
6. Boudoulas KD, Montague CR, Goldschmidt-Clermont PJ, Cooke GE. Physiological Estradiol Increases
Aggregation of PlA1/A1
Platelets. 2006. Am Heart J 152:136-9.
7. Chotani MA, Mitra S, Su BY, Flavahan S, Eid AH, Clark KR, Montague CR, Paris H, Handy DE, Flavahan
NA. Regulation of 2-adrenoceptors in human vascular smooth muscle cells. 2004. American Journal of
Physiology: Heart Circulatory Physiology 286:H59-67.
8. Eubank TD, Galloway M, Montague CM, Waldman WJ, Marsh CB. M-CSF induces vascular endothelial
growth factor production and angiogenic activity from human monocytes. 2003. Journal of Immunology
171:2637-43.
9. Boudoulas KD, Cooke GE, Roos CM, Bray PF, Goldschmidt-Clermont PJ. The PlA2
polymorphism of
glycoprotein IIIa functions as a modifier for the effect of estrogen on platelet aggregation. 2001. Archives of
Pathology and Laboratory Medicine 125:112-115.
10. Vijayan KV, Goldschmidt-Clermont PJ, Roos C, Bray PF. The PlA2
polymorphism of integrin 3 enhances
outside-in signaling and adhesive functions. 2000. Journal of Clinical Investigation 105:793-802.
11. Ying AK, Hassanain HH, Roos CM, Smiraglia DJ, Issa JJ, Michler RE, Caligiuri M, Plass C, Goldschmidt-
Clermont PJ. Methylation of the estrogen receptor-alpha gene promoter is selectively increased in
proliferating human aortic smooth muscle cells. 2000. Cardiovascular Research 46:172-179.
12. Goldschmidt-Clermont PJ, Roos CM, Cooke GE. Platelet PlA2
polymorphism and thromboembolic events:
from inherited risk to pharmacogenetics. 1999. Journal of Thrombosis and Thrombolysis 8:89-103.
13. Irani K, Pham Y, Coleman L, Roos C, Cooke G, Miodovnik A, Karim N, Wilhide C, Bray P, Goldschmidt-
Clermont P. Priming of platelet IIb3 by oxidants is associated with tyrosine phosphorylation of 3. 1998.
Arteriosclerosis Thrombosis and Vascular Biology 18:1698-1706.
14. Frank DU, Lowson SM, Roos CM, Rich GF. Endotoxin alters hypoxic pulmonary vasoconstriction in
isolated rat lungs. 1996. Journal of Applied Physiology 81:1316-1322.
15. Uncles DR, Daugherty MO, Frank DU, Roos CM, Rich GF. Nitric oxide modulation of pulmonary vascular
resistance is red blood cell dependent in isolated rat lungs. 1996. Anesthesia and Analgesia 83:1212-1217.
16. Roos CM, Frank DU, Xue C, Johns RA, Rich GF. Chronic inhaled nitric oxide: effects on pulmonary
vascular endothelial function and pathology in hypoxic and normoxic rats. 1996. Journal of Applied
Physiology 80:252-260.
17. Anderson SM, Rich GF, Roos CM, Lee LP, Lee JS. Fluid restitution and blood volume change in
anesthetized rabbits in response to vasoactive drugs. 1994. Circulation 90:509-514.
18. Roos CM, Rich GF, Uncles DR, Daugherty MO, Frank DU. Sites of vasodilation by inhaled nitric oxide
versus sodium nitroprusside in endothelin constricted isolated rat lungs. 1994. Journal of Applied
Physiology 77:51-57.
19. Rich GF, Roos CM, Anderson SM, Daugherty MO, Uncles DR. Direct effects of intravenous anesthetics on
pulmonary vascular resistance in the isolated rat lung. 1994. Anesthesia and Analgesia 78:961-966.
20. Rich GF, Murphy GD, Roos CM, Johns RA. Inhaled nitric oxide: selective pulmonary vasodilation in cardiac
surgical patients. 1993. Anesthesiology 78:1028-1035.
21. Rich GF, Roos CM, Anderson SM, Urich DC, Daugherty MO, Johns RA. Inhaled nitric oxide: dose
response and the effects of blood in the isolated rat lung. 1993. Journal of Applied Physiology 75:1278-
1284.