We are pleased to share the documents presented at the round table discussion on “Vineyard and climate change”, in which Enrico Peterlungen, José Ramón Lisarrague and Gregory Jones participated on the 2nd day of the #10thWBWE

1. VINEYARD AND CLIMATE CHANGE
ROUND TABLE
ENRICO PETERLUNGER
WBWE – 10th World Bulk Wine Exhibition
26-27 November 2018
Amsterdam RAI, Netherlands
hic sunt futura
Department of Food, Agriculture.
Environmental and Animal
Sciences

2. [CO2] 1980  330 ppm
2018  400 ppm

5. UNIVERSITY
OF UDINE
ROME
REGION
FRIULI VENEZIA GIULIA

6. Increase of temperature in the region Friuli-Venezia Giulia,
North Eastern Italy (OSMER 2012)

8. Climate change in FVG: Degree-days
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
1996 1998 2000 2002 2004 2006 2008 2010 2012
Gradigiorno(C°)
Anno
Cumulated degree-days since 1996 till 2013:
from 1600 up to 1900 DD

9. Climate change in FVG: rainfall
0
50
100
150
200
250
aprile maggio giugno luglio agosto settembre
Precipitazionimensili(mmmese-1)
1994-2003 2004-2013
118
120
122
124
126
128
130
132
134
136
138
1994-2003 2004-2013
Numerodigiornisecchi
Monthly rainfall and number of dry days:
comparison 1994-2003 vs 2004-2013;
Differences: not statistically different

10. Harvest date in Friuli
 advance
In last 20 years
 - 10 days
advance = 0,5 d / year

11. How to cope with this?
- Adapt cultivation techniques
-  pruning
-  delay budburst
-  water supply management
- Breeding new genotypes
increase sustainability

12. Tradition vs. Innovation:
is it a real contrast?
Tradition is ……
a successful innovation!

13. Grapevine breeding at the
UNIVERSITY of UDINE

14. viticulture today in EU
• Viticulture has a significative impact on environment
• in Europe it occupies 3 % arable land and uses 65% of
all fungicides (68.000 t/year) (EURTOSTAT 2007)
• In grapevine breeding was limited by traditional
cultivar importance

15. The steps of Udine University
grapevine breeding program
1998 Collection of genetic material in the experimental farm of
University of Udine
2002 Start of genetic studies (resistance genetic map, marker
assisted selection …)
2005 Start of agronomic selection
2006 Start of Institute of Applied Genomics IGA
Grapevine genome sequencing
2007 Start of nano-vinification and wine evaluation
2011 1° selection cycle finished, open field demonstration
vineyards
2013
2015
Presentation to Italian Ministry of Agriculture of
application te register 10 cvs (5 white and 5 red)
registration of 10 cvs in Europe (CPVO) and in Italy

16. Grapevine genome revealed more than 500 resistance genes,
however V. vinifera is not resistant to downy and powdery
mildew

17. 3. the resistance to downy & powdery mildew
Pathogen Gene Chromosome Source Reference
Plasmopara Rpv1 12 M. rotundifolia Blanc et al 2012
Rpv2 18 M. rotundifolia Blanc et al 2012
Rpv3 18 V. rupestris Di Gaspero et al 2011
Rpv8 14 V. amurensis Blasi et al 2011
Rpv10 9 V. amurensis Schwander et al 2011
Rpv12 14 V. amurensis Venuti et al 2013
Oidium Run1 12 M. rotundifolia Pauquet et al 2001
Run2 18 M. rotundifolia Riaz et al 2011
Ren1 13 V. vinifera Coleman et al 2011
Ren4 18 V. romanetii Mahanil et al 2011
Ren5 14 M. rotundifolia Blanc et al 2012
most disease-resistance genes come from wild American and Asian species, but the Ren1
gene, that confers resistance to powdery mildew, has been found in V. vinifera cv Kishmish
vatkana. Resistance to downy mildew was also found in a vinifera variety Mgaloblishvili
from Georgia (Caucasus).

18. The activities and the numbers of the project
800+ grapevine accessions introduced in our collection
365 crossing combinations
25.000+ seedlings evaluated
400+ nano- and micro-vinifications at UIV Verona and VCR
10 new varieties selectioned for resistance to diseases,
agronomical characters and wine quality, registered
at EU-CPVO and MIPAAF-Italy

19. From the project archive

20. 80,024 76,026 34,113 34,111 55,100
32,078 31,103 31,125 31,122 72,096
some selections

21. The choice of parents
susceptible (S)
Chardonnay
Cabernet S.
Merlot
Sauvignon
Sangiovese
Tocai
...
resistant (R)
Bianca
20/3
Regent
Seyval
Pannonia
SK-00-1/2
...

22. crossing and cultivation of seedlings

23. selection for resistance
Zarja severa
(Rpv12)
↓
20-3
Villard blanc
(Rpv13)
↓
Bianca, Regent
Severnyi
(Rpv10)
↓
Bronner, Solaris

24. selection for agronomical characters
vigor (average)
productivity (average)
cluster (preferably loose)

25. Selection for enological characters
463 nanovinifications (1-2 L) till 2010 at UIV - Verona
10 best selections have been registered on 2015 at National
Grapevine Catalog at the MIPAAF
expert panel
sensory evaluation
Micro
vinifications
HPLC & GC-MS analysis

26. the white varieties registered in 2015
Selection Name Cross
34.111 Fleurtai Tocai x 20/3
34.113 Soreli Tocai x 20/3
76.026 Sauvignon Kretos Sauvignon x 20/3
55.098 Sauvignon Nepis Sauvignon x Bianca
55.100 Sauvignon Rytos Sauvignon x Bianca
Fleurtai Soreli Sauvignon Sauvignon Sauvignon
Kretos Nepis Rytos

27. Cluster wt.: 194g
SAUVIGNON RYTOS
Sauvignon x Bianca
55.100
Yield: 11.6 t/ha
YEAR TA DRY EXTRACT ALCOHOL %
g/L g/ L
2012 6,28 22,9 14
2013 6,05 19,6 14
2014 5,2 18,1 12,2

29. SORELI
Tocai Friulano x 20-3 (34.113)
Cluster wt.: 173g
YIELD: 10 t/ha
YEAR TA DRY EXTRACT ALCOHOL %
g/L g/L
2012 5,67 19,9 13,1
2013 5,61 18,7 13,6
2014 5,5 18,2 12,3

31. MERLOT KHORUS
Merlot x 20-3 (31.125)
Cluster wt.: 139 g
YIELD: 9.7 t/ha
YEAR TA DRY EXTRACT ALCOHOL ANTH. POLYPHEN.
g/L g/L % mg/L mg/L
2012 5,2 38,6 14,2 1080 3650
2013 5,4 35,7 14,0 958 4203
2014 5,2 34,7 13,3 879 3131

32. -for ageing (barrique)

33. CABERNET VOLOS
Cabernet Sauvignonx20-3
(32.078)
Cluster wt.: 156g
YIELD: 11.9 t/ha
YEAR TA DRY EXTRACT ALCOHOL ANTH. POLYPHEN.
g/L g/L % mg/L mg/L
2012 5,5 32,2 13,9 1267 4300
2013 5,5 31,6 12,9 1213 3751
2014 5,2 30,3 12,8 648 2497

34. -for ageing (barrique)-for ageing (barrique)

35. methanol
No FOXY AROMA in the new resistant grapevines

36. future developments
Combine resistance genes
• 3 resistances to dawny mildew (rpv3, rpv10,
rpv12)
• 2 resistances to powdery mildew (ren1, run1)
• Other resistances
differentiate the product
• sparkling wine
• wines to be aged
• dessert wines
• table grapes
…….
%
16.26
new tools for selection
• use of grapevine genome sequence for
selection
• analysis of metabolic profiles of primary
and secondary metabolites
Agronomical features
• late budburst
• early harvest
• late harvest
• high yield

37. Cisgenic transformation
• Transferring genes from another species
of Vitis genus
Genome editing
• CRISPR technology
• Modifying one nucleotide to silence one gene, e.g. a
gene involved in pathogen/host mutual recognition

38. Paolo Barbara Orietta Silvia Moreno ………….. Michela Francesco
Diana Elisa Luigi Courtney Dario Serena Renato Giorgio
Guido Gabriele Simone
Michele Enrico Raffaele
collaborators to the project

39. Collaborations with institutions
• Institute of Viticulture, Pécs, Hungary
• Missouri State University, USA
• INRA, Colmar, France
• Université de Strasbourg, France
• Genoscope, Paris, France
• Institut für Rebenzüchtung, Geilweilerhof, Germany
• Hochschule Geisenheim University, Germany
• UIV, Unione Italiana Vini, Verona, Italy
• Università di Verona, Italy
• CRA Istituto di Viticoltura, Conegliano, Italy
• University of Novi Sad, Serbia
Support and funding
• Università di Udine / Azienda agraria «A. Servadei»
• Regione Friuli Venezia Giulia
• MiPAF Progetti Vigna & Vigneto
• MiUR progetti nazionali PRIN
• Vivai Cooperativi di Rauscedo
• Banche di Credito Cooperativo del FVG
• Fondazioni bancarie CRUP, CRT, CARIGO
• Consorzio Collio
• Vignaioli: Felluga L, Felluga M, Zamò, Venica & Venica

40. THANKS FOR
YOUR ATTENTION!
hic sunt futura
In collaboration
with