Helps in understanding the concept in better way of plant seed borne diseases

1. Batch No : 1
Roll No : 01 to 09
SEVENTH SEMESTER B.Sc. (Hons.) Agriculture
RAWE & AIA Programme
ON CAMPUS TRAINING PHASE
SUBJECT : PLANT
PATHOLOGY
Submitted to : Dr. Rangnathswami Math
Year: 2021 - 2022
(During 17-08-2021 to 09-09-2021)

2. INSTRUMENTS REQUIRED DURING
CULTURE
 Seeds of Soybean
 Petri Plates
 Tissue papers
 Nalco solution

3. Distilled water
Autoclaved water in flask
Laminar Air Flow Agar Media

4. PROCEDURE –
Isolation of internal seed borne disease
Step 1 : Clean the working area with alcohol to avoid other microbial
contaminations.
Step 2 : Pour Sodium hypochlorite solution in first Petri plate.
Step 3 : Pour autoclaved distilled water in another three Petri plates.
Step 4 :Burn the spirit lamp behind the wooden slate for avoid contamination of
germs.

5. Pour the seeds in sodium
hypochlorite solution for
about 3 minutes.
After pouring the seeds in
solution wash it with distilled
water 3 times, in each for 1 to
1.5 minutes.

6.  After transferring the seeds to 4th plate remove it after 2 minutes.
 Take out the wet seeds from plate with the help of pinchers.
 Transfer the seeds to tissue paper.
 Let them soak for 2 minutes.

7. After soaking of seeds
transfer it to the agar
plate with the help of
pinchers, one by one.
Put seeds with maximum
space for proper growth
of disease.
After completing the
transfer of seeds to media
cover it with paraffin wax
and cover it properly.
Cover the plate properly.

8. THANK YOU