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MASS PRODUCTION OF TRICHOGRAMMA
CHILONIS
Prepared by
Mr. Mahesh Vikram Sable
Assistant Professor
Department of Plant Pathology
College of Agriculture, Ashti
 Rice moth i.e. Corcyra cephalonica is used as a laboratory
host for the production of natural enemies like
Trichogramma, Crytolaemas and Crysoperla
PRODUCTION PROCEDURE OF CORCYRA
CEPHALONICA
 Bold white sorghum grains used for human
consumption can be used and it should not treated
with insecticides.
 The required quantity of sorghum is milled to make
3-4 pieces of each grain.
 Sorghum is heat sterilized in oven at 1000 C for 30
minutes
 Sorghum is sprayed with 0.1 % formalin and air
dried to prevent the growth of moulds and decrease
the grain humidity.
Milled
Sorghum
0.1 %
Formalin
Air
Drying
 Sorghum is poured @ 2.5 kg / Corcyra box
 Thoroughly mixed 100 g groundnut karnel extract +
1 g sulphur dust to avoid mite infection.
100 g
Ground
Nut Karnel
Extract
1 g
Sulphur
Dust
Avoid mite
infection
 To start with 600 boxes (Each containing 2.5 kg of
sorghum / box) are infested with 300 cc of fresh laid
Corcyra egg (i.e. 0.5 cc / box) and the lid is secured
for about 30days.
 The 600 boxes is kept in rack and lid closed, on
40th day moth start emerging and emerging
continues for about two months.
 10 to 75 moth emerge daily with peak moth
emergence being between 65th and 75th day.
 The moth are collected daily and transferred to the
specially designed oviposition cages.
 Moth emergence reduces after 100 days of initial
infestation and boxes are reused after cleaning.
 Provide cotton soaked 20% honey + vitamin E solution
as adult food in the egg laying chamber and collect the
eggs daily.
 Moth emergence reduces after 100 days of initial
infestation and boxes are reused after cleaning.
 Egg which pass through 15, 30 and 40 mesh sieve for
cleaning and are collected and run over a slope of paper
to eliminate dust particles.
 The egg are treated with UV rays.
 15 wt UV tube for 15 minutes at a distances of 2 feet to
prevent hatching.
 Utilize the Corcyra eggs for Trichogramma production
(or) host culture or store them in refrigerator at 10
degree centigrade for 7 days, if required
MASS PRODUCTION OF TRICHOGRAMMA
 Materials required
1. Corcyra eggs
2. Nucleus culture of Trichogramma
3. Polythene bags
4. Rubber bands
5. Scissors
6. Gum
7. Brush
8. Tea strainer
9. Tricho cards
10. 50% honey solution
11. Stapler
12. Refrigerator
13. UV lamp
PROCEDURE
 Clean fresh Corcyra eggs by passing through 15, 30 and
45 mesh sieves.
 Prepare “Trichocard” by cutting card board sheet to the
size of 10 x10 cm which can accommodate 1 cc of eggs.
 Apply gum on the card and sprinkle the cleaned eggs
uniformly. Remove the excess eggs from the cards by
using brush.
 Allow the card for shade drying for 30 minutes.
 Treat the eggs under UV lamp for 30 minutes.
 Take polythene bag, insert UV treated “Trichocard” and
nucleus card at the ratio of 6:1 (6 Corcyra egg cards:
1 Trichogramma nucleus card) and provide 50% honey
vitamin E in a soaked cotton swab.
 Remove the Tricho cards after 2 days Corcyra eggs
changes black color on 3rd day indicates the
parasitization of eggs.
 Release the parasitized egg cards immediately in the
fields (or) store them in refrigerator at 10 degree
centigrade up to 21 days. Place/tie/staple parasitized
cards on leaf sheath of plant.
THANK
YOU

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Mass producation of trichogrmma

  • 1. MASS PRODUCTION OF TRICHOGRAMMA CHILONIS Prepared by Mr. Mahesh Vikram Sable Assistant Professor Department of Plant Pathology College of Agriculture, Ashti
  • 2.  Rice moth i.e. Corcyra cephalonica is used as a laboratory host for the production of natural enemies like Trichogramma, Crytolaemas and Crysoperla
  • 3. PRODUCTION PROCEDURE OF CORCYRA CEPHALONICA  Bold white sorghum grains used for human consumption can be used and it should not treated with insecticides.
  • 4.  The required quantity of sorghum is milled to make 3-4 pieces of each grain.  Sorghum is heat sterilized in oven at 1000 C for 30 minutes  Sorghum is sprayed with 0.1 % formalin and air dried to prevent the growth of moulds and decrease the grain humidity. Milled Sorghum 0.1 % Formalin Air Drying
  • 5.  Sorghum is poured @ 2.5 kg / Corcyra box  Thoroughly mixed 100 g groundnut karnel extract + 1 g sulphur dust to avoid mite infection. 100 g Ground Nut Karnel Extract 1 g Sulphur Dust Avoid mite infection
  • 6.  To start with 600 boxes (Each containing 2.5 kg of sorghum / box) are infested with 300 cc of fresh laid Corcyra egg (i.e. 0.5 cc / box) and the lid is secured for about 30days.  The 600 boxes is kept in rack and lid closed, on 40th day moth start emerging and emerging continues for about two months.
  • 7.  10 to 75 moth emerge daily with peak moth emergence being between 65th and 75th day.  The moth are collected daily and transferred to the specially designed oviposition cages.  Moth emergence reduces after 100 days of initial infestation and boxes are reused after cleaning.
  • 8.  Provide cotton soaked 20% honey + vitamin E solution as adult food in the egg laying chamber and collect the eggs daily.  Moth emergence reduces after 100 days of initial infestation and boxes are reused after cleaning.  Egg which pass through 15, 30 and 40 mesh sieve for cleaning and are collected and run over a slope of paper to eliminate dust particles.
  • 9.  The egg are treated with UV rays.  15 wt UV tube for 15 minutes at a distances of 2 feet to prevent hatching.  Utilize the Corcyra eggs for Trichogramma production (or) host culture or store them in refrigerator at 10 degree centigrade for 7 days, if required
  • 10. MASS PRODUCTION OF TRICHOGRAMMA  Materials required 1. Corcyra eggs 2. Nucleus culture of Trichogramma 3. Polythene bags 4. Rubber bands 5. Scissors 6. Gum 7. Brush 8. Tea strainer 9. Tricho cards 10. 50% honey solution 11. Stapler 12. Refrigerator 13. UV lamp
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  • 12. PROCEDURE  Clean fresh Corcyra eggs by passing through 15, 30 and 45 mesh sieves.  Prepare “Trichocard” by cutting card board sheet to the size of 10 x10 cm which can accommodate 1 cc of eggs.
  • 13.  Apply gum on the card and sprinkle the cleaned eggs uniformly. Remove the excess eggs from the cards by using brush.  Allow the card for shade drying for 30 minutes.  Treat the eggs under UV lamp for 30 minutes.  Take polythene bag, insert UV treated “Trichocard” and nucleus card at the ratio of 6:1 (6 Corcyra egg cards: 1 Trichogramma nucleus card) and provide 50% honey vitamin E in a soaked cotton swab.
  • 14.  Remove the Tricho cards after 2 days Corcyra eggs changes black color on 3rd day indicates the parasitization of eggs.  Release the parasitized egg cards immediately in the fields (or) store them in refrigerator at 10 degree centigrade up to 21 days. Place/tie/staple parasitized cards on leaf sheath of plant.
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