1. Comparison between culture dependent and culture indipendent analyses of
Lactobacillus plantarum in stool of apparently healthy adult supplemented
with Lactobacillus plantarum IS-10506 from Dadih
Azmier Adib1, Mardiastuti H Wahid2, Pratiwi Sudarmono2 and Ingrid S
Surono3
1Freelance,2Departement of Microbiology, Medical Faculty, University of Indonesia,
3 BINUS University / SEAMEO RECFON- UI
e-mail: gue_gondronk@yahoo.com

2. Introduction
Dadih  traditional food
 health benefit  antimutagenic,
antipathogenic  S.aureus DSM 20231,
E.sakazaki ATCC 29544,
C.difficile DSM 1296, E.coli K2, & local pathogens
 Food Industry  Lactobacillus plantarum IS-10506  one of dominant
probiotic of Dadih

3. Aim
 To compare between culture dependent and culture independent
analyses as well as to know the viability and availability of
Lactobacillus plantarum in Stool of Apparently Healthy Adults
Consuming Lactobacillus plantarum IS-10506 from Dadih

4. Method
 Culture dependent
 Total plate count on MRS agar + Bromo Cresol Purple 
Lactobacillus sp  Yellow
 Culture independent analyses  qPRC  specific Primer planF and pREV 
Ct Value.
-. Low number of Ct value = more number of DNA
-. CT value is the cycle at which a significant increase in the first of
Delta Rn detected,
CT value is the parameter used for quantitation .
-. Delta Rn value of a reaction containing all components (the
sample of interest).
0 10 20 30 40
cyclenumber
Rn
CT
Threshold Rn
Sample
NoTemplate
Control
WhatisRn?
-Rn
+Rn

5. 0
20
40
60
80
100
120
140
160
180
BL W0 Pr WI WII WIII
Probiotic
33.5
34
34.5
35
35.5
36
36.5
37
37.5
38
38.5
Bl Pr W I W I W II W III
culture dependent & culture independent analyses
•Fecal lactic bacteria were significantly higher in Ct value on probiotic group by culture independent methods detected
compared to placebo group (p<0.05).
•Culture dependent showed that viable fecal lactic acid bacteria tend to be higher in probiotic group than in placebo, but not
significant.
culture independent analyses

6. 38.85
38.9
38.95
39
39.05
39.1
39.15
39.2
39.25
39.3
Bl Pl W 0 W I W II W III
culture dependent & culture independent analyses
culture dependent culture independent analyses
0
20
40
60
80
100
120
140
160
180
BL W0 Pl W I W II W III

7. 121
121.5
122
122.5
123
123.5
I-III Pr I-III PL
34.5
35
35.5
36
36.5
37
37.5
38
38.5
39
39.5
W I-III Pr W I-III Pl
Culture independent analyses more accurate because the VBNC
may not be cultured
Comparison between Probiotic and
Placebo groupsculture dependent culture independent analyses

8. Conclusion
 The results showed that fecal lactic bacteria were significantly
higher in Ct value on probiotic group by culture independent
methods detected compared to placebo group (p<0.05).
 Culture dependent showed that viable fecal lactic acid bacteria
tend to be higher in probiotic group than in placebo, but not
significant (p>0.05).
 Culture independent analyses more accurate because the VBNC
may not be cultured.