SlideShare a Scribd company logo
1 of 1
Iván García<br />PGlo SDS Page:<br />SDS page was basically an extension of the overall pGlo experiment. This SDS page was conducted on proteins using the electrophoresis technique. The bacteria used in the previous experiment were used as protein samples in our SDS page. An SDS Page uses an agar gel, which is then loaded with the samples and in each corner is loaded with ladders to serve as controls. Basically the electrophoresis drags down the denatured protein depending on their weight; the further down they travel the lighter they are. This workshop was provided by Dr. Chiesa and was useful in determining which proteins contained GFP and which didn’t express the GFP gene. <br />

More Related Content

What's hot

Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...
Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...
Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...St John's Laboratory Ltd
 
Cataloging computationally putative resistance genes in grape and
Cataloging computationally putative resistance genes in grape andCataloging computationally putative resistance genes in grape and
Cataloging computationally putative resistance genes in grape andAmer T. Wazwaz
 
pGLO Tutorial - English
pGLO Tutorial - English pGLO Tutorial - English
pGLO Tutorial - English Mills Cbst
 
2013 UW Grad Conference
2013 UW Grad Conference2013 UW Grad Conference
2013 UW Grad Conferenceitskathylam
 
Clinical Application of RNA Sequencing - Bladder Cancer
Clinical Application of RNA Sequencing - Bladder CancerClinical Application of RNA Sequencing - Bladder Cancer
Clinical Application of RNA Sequencing - Bladder CancerUSD Bioinformatics
 
CMB Final Presentation
CMB Final Presentation CMB Final Presentation
CMB Final Presentation Bella Bobrow
 
Nervni sistem slike
Nervni sistem  slikeNervni sistem  slike
Nervni sistem slikedr Šarac
 
Next Generation Sequencing - the basics
Next Generation Sequencing - the basicsNext Generation Sequencing - the basics
Next Generation Sequencing - the basicsUSD Bioinformatics
 
Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...
Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...
Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...Nacho Caballero
 
next generation sequemcing
next generation sequemcingnext generation sequemcing
next generation sequemcingShahzebkhan135
 
Recombinant Antibody Overview I - Creative Biolabs
Recombinant Antibody Overview I - Creative BiolabsRecombinant Antibody Overview I - Creative Biolabs
Recombinant Antibody Overview I - Creative BiolabsCreative-Biolabs
 

What's hot (18)

Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...
Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...
Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) ...
 
Cataloging computationally putative resistance genes in grape and
Cataloging computationally putative resistance genes in grape andCataloging computationally putative resistance genes in grape and
Cataloging computationally putative resistance genes in grape and
 
pGLO Tutorial - English
pGLO Tutorial - English pGLO Tutorial - English
pGLO Tutorial - English
 
2013 UW Grad Conference
2013 UW Grad Conference2013 UW Grad Conference
2013 UW Grad Conference
 
Bacterial genetics
Bacterial geneticsBacterial genetics
Bacterial genetics
 
Clinical Application of RNA Sequencing - Bladder Cancer
Clinical Application of RNA Sequencing - Bladder CancerClinical Application of RNA Sequencing - Bladder Cancer
Clinical Application of RNA Sequencing - Bladder Cancer
 
kkyle_RevisedPres2015_FINAL
kkyle_RevisedPres2015_FINALkkyle_RevisedPres2015_FINAL
kkyle_RevisedPres2015_FINAL
 
CMB Final Presentation
CMB Final Presentation CMB Final Presentation
CMB Final Presentation
 
Nervni sistem slike
Nervni sistem  slikeNervni sistem  slike
Nervni sistem slike
 
Next Generation Sequencing - the basics
Next Generation Sequencing - the basicsNext Generation Sequencing - the basics
Next Generation Sequencing - the basics
 
Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...
Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...
Using the Host Immune Response to Hemorrhagic Fever Viruses to Understand Pat...
 
next generation sequemcing
next generation sequemcingnext generation sequemcing
next generation sequemcing
 
Pglo transformation
Pglo transformationPglo transformation
Pglo transformation
 
Presentacion seminario
Presentacion seminario Presentacion seminario
Presentacion seminario
 
AdenugaSMART2009
AdenugaSMART2009AdenugaSMART2009
AdenugaSMART2009
 
Recombinant Antibody Overview I - Creative Biolabs
Recombinant Antibody Overview I - Creative BiolabsRecombinant Antibody Overview I - Creative Biolabs
Recombinant Antibody Overview I - Creative Biolabs
 
Human Cloning
Human CloningHuman Cloning
Human Cloning
 
Paper plasmid lab
Paper plasmid labPaper plasmid lab
Paper plasmid lab
 

Viewers also liked (20)

Power point rosario
Power point rosarioPower point rosario
Power point rosario
 
Canadian Artists Project By Rachel T
Canadian Artists Project By Rachel TCanadian Artists Project By Rachel T
Canadian Artists Project By Rachel T
 
Muñequeria artistica
Muñequeria artisticaMuñequeria artistica
Muñequeria artistica
 
Slidehare
SlidehareSlidehare
Slidehare
 
Tabla de word
Tabla de wordTabla de word
Tabla de word
 
Mercadillo solidario 2011
Mercadillo solidario 2011Mercadillo solidario 2011
Mercadillo solidario 2011
 
Curso mapa
Curso mapaCurso mapa
Curso mapa
 
Bloque Academico por Anabel Abarca
Bloque Academico por Anabel AbarcaBloque Academico por Anabel Abarca
Bloque Academico por Anabel Abarca
 
Historia de los Gunners
Historia de los GunnersHistoria de los Gunners
Historia de los Gunners
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
LA WEB-2
LA WEB-2 LA WEB-2
LA WEB-2
 
La ciudad de paris
La ciudad de parisLa ciudad de paris
La ciudad de paris
 
Its Time To Go And Play
Its Time To Go And PlayIts Time To Go And Play
Its Time To Go And Play
 
Diapositivas roxana
Diapositivas roxanaDiapositivas roxana
Diapositivas roxana
 
Trabajo numero 1 david aguilera y nicolle munevar
Trabajo numero 1 david aguilera y nicolle munevarTrabajo numero 1 david aguilera y nicolle munevar
Trabajo numero 1 david aguilera y nicolle munevar
 
Jorge david
Jorge   davidJorge   david
Jorge david
 
Croquis paises
Croquis paisesCroquis paises
Croquis paises
 
Annoted bibliography 3
Annoted bibliography 3Annoted bibliography 3
Annoted bibliography 3
 
Relative clauses
Relative clausesRelative clauses
Relative clauses
 
Encuestas
EncuestasEncuestas
Encuestas
 

More from Aquilles07

A lab on a chip opinion revisedfinal
A lab on a chip opinion revisedfinalA lab on a chip opinion revisedfinal
A lab on a chip opinion revisedfinalAquilles07
 
Annoted bibliography#2
Annoted bibliography#2Annoted bibliography#2
Annoted bibliography#2Aquilles07
 
Review paper revised final
Review paper revised finalReview paper revised final
Review paper revised finalAquilles07
 
Pathophysiological significance of neuronal nitric oxide synthase in the gast...
Pathophysiological significance of neuronal nitric oxide synthase in the gast...Pathophysiological significance of neuronal nitric oxide synthase in the gast...
Pathophysiological significance of neuronal nitric oxide synthase in the gast...Aquilles07
 
Dna in technology
Dna in technologyDna in technology
Dna in technologyAquilles07
 
Snake venom immunology proposal
Snake venom immunology proposalSnake venom immunology proposal
Snake venom immunology proposalAquilles07
 
F inal presentation ivan j.
F inal presentation ivan j.F inal presentation ivan j.
F inal presentation ivan j.Aquilles07
 
Snake venom immunology proposal
Snake venom immunology proposalSnake venom immunology proposal
Snake venom immunology proposalAquilles07
 
Snake venom-immunology33
Snake venom-immunology33Snake venom-immunology33
Snake venom-immunology33Aquilles07
 
Sodis effectiveness-in-water-purification-revised1
Sodis effectiveness-in-water-purification-revised1Sodis effectiveness-in-water-purification-revised1
Sodis effectiveness-in-water-purification-revised1Aquilles07
 
Solar water disinfection in rural puerto rican counties
Solar water disinfection in rural puerto rican countiesSolar water disinfection in rural puerto rican counties
Solar water disinfection in rural puerto rican countiesAquilles07
 

More from Aquilles07 (20)

A lab on a chip opinion revisedfinal
A lab on a chip opinion revisedfinalA lab on a chip opinion revisedfinal
A lab on a chip opinion revisedfinal
 
Annoted bibliography#2
Annoted bibliography#2Annoted bibliography#2
Annoted bibliography#2
 
Review paper revised final
Review paper revised finalReview paper revised final
Review paper revised final
 
Pathophysiological significance of neuronal nitric oxide synthase in the gast...
Pathophysiological significance of neuronal nitric oxide synthase in the gast...Pathophysiological significance of neuronal nitric oxide synthase in the gast...
Pathophysiological significance of neuronal nitric oxide synthase in the gast...
 
Dna in technology
Dna in technologyDna in technology
Dna in technology
 
Armaiz
ArmaizArmaiz
Armaiz
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
Snake venom immunology proposal
Snake venom immunology proposalSnake venom immunology proposal
Snake venom immunology proposal
 
F inal presentation ivan j.
F inal presentation ivan j.F inal presentation ivan j.
F inal presentation ivan j.
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
Snake venom immunology proposal
Snake venom immunology proposalSnake venom immunology proposal
Snake venom immunology proposal
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
Snake venom-immunology33
Snake venom-immunology33Snake venom-immunology33
Snake venom-immunology33
 
Sodis effectiveness-in-water-purification-revised1
Sodis effectiveness-in-water-purification-revised1Sodis effectiveness-in-water-purification-revised1
Sodis effectiveness-in-water-purification-revised1
 
Solar water disinfection in rural puerto rican counties
Solar water disinfection in rural puerto rican countiesSolar water disinfection in rural puerto rican counties
Solar water disinfection in rural puerto rican counties
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
Microscopy
MicroscopyMicroscopy
Microscopy
 
Nano tech.
Nano tech.Nano tech.
Nano tech.
 
Nano tech
Nano techNano tech
Nano tech
 

Recently uploaded

Platformless Horizons for Digital Adaptability
Platformless Horizons for Digital AdaptabilityPlatformless Horizons for Digital Adaptability
Platformless Horizons for Digital AdaptabilityWSO2
 
TrustArc Webinar - Unlock the Power of AI-Driven Data Discovery
TrustArc Webinar - Unlock the Power of AI-Driven Data DiscoveryTrustArc Webinar - Unlock the Power of AI-Driven Data Discovery
TrustArc Webinar - Unlock the Power of AI-Driven Data DiscoveryTrustArc
 
AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)
AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)
AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)Samir Dash
 
Strategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
Strategize a Smooth Tenant-to-tenant Migration and Copilot TakeoffStrategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
Strategize a Smooth Tenant-to-tenant Migration and Copilot Takeoffsammart93
 
Artificial Intelligence Chap.5 : Uncertainty
Artificial Intelligence Chap.5 : UncertaintyArtificial Intelligence Chap.5 : Uncertainty
Artificial Intelligence Chap.5 : UncertaintyKhushali Kathiriya
 
Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...
Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...
Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...apidays
 
Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...
Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...
Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...Jeffrey Haguewood
 
presentation ICT roal in 21st century education
presentation ICT roal in 21st century educationpresentation ICT roal in 21st century education
presentation ICT roal in 21st century educationjfdjdjcjdnsjd
 
Finding Java's Hidden Performance Traps @ DevoxxUK 2024
Finding Java's Hidden Performance Traps @ DevoxxUK 2024Finding Java's Hidden Performance Traps @ DevoxxUK 2024
Finding Java's Hidden Performance Traps @ DevoxxUK 2024Victor Rentea
 
JohnPollard-hybrid-app-RailsConf2024.pptx
JohnPollard-hybrid-app-RailsConf2024.pptxJohnPollard-hybrid-app-RailsConf2024.pptx
JohnPollard-hybrid-app-RailsConf2024.pptxJohnPollard37
 
"I see eyes in my soup": How Delivery Hero implemented the safety system for ...
"I see eyes in my soup": How Delivery Hero implemented the safety system for ..."I see eyes in my soup": How Delivery Hero implemented the safety system for ...
"I see eyes in my soup": How Delivery Hero implemented the safety system for ...Zilliz
 
AWS Community Day CPH - Three problems of Terraform
AWS Community Day CPH - Three problems of TerraformAWS Community Day CPH - Three problems of Terraform
AWS Community Day CPH - Three problems of TerraformAndrey Devyatkin
 
Introduction to Multilingual Retrieval Augmented Generation (RAG)
Introduction to Multilingual Retrieval Augmented Generation (RAG)Introduction to Multilingual Retrieval Augmented Generation (RAG)
Introduction to Multilingual Retrieval Augmented Generation (RAG)Zilliz
 
Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024
Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024
Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024Victor Rentea
 
ICT role in 21st century education and its challenges
ICT role in 21st century education and its challengesICT role in 21st century education and its challenges
ICT role in 21st century education and its challengesrafiqahmad00786416
 
How to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerHow to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerThousandEyes
 
FWD Group - Insurer Innovation Award 2024
FWD Group - Insurer Innovation Award 2024FWD Group - Insurer Innovation Award 2024
FWD Group - Insurer Innovation Award 2024The Digital Insurer
 
Six Myths about Ontologies: The Basics of Formal Ontology
Six Myths about Ontologies: The Basics of Formal OntologySix Myths about Ontologies: The Basics of Formal Ontology
Six Myths about Ontologies: The Basics of Formal Ontologyjohnbeverley2021
 
Cloud Frontiers: A Deep Dive into Serverless Spatial Data and FME
Cloud Frontiers:  A Deep Dive into Serverless Spatial Data and FMECloud Frontiers:  A Deep Dive into Serverless Spatial Data and FME
Cloud Frontiers: A Deep Dive into Serverless Spatial Data and FMESafe Software
 
Introduction to use of FHIR Documents in ABDM
Introduction to use of FHIR Documents in ABDMIntroduction to use of FHIR Documents in ABDM
Introduction to use of FHIR Documents in ABDMKumar Satyam
 

Recently uploaded (20)

Platformless Horizons for Digital Adaptability
Platformless Horizons for Digital AdaptabilityPlatformless Horizons for Digital Adaptability
Platformless Horizons for Digital Adaptability
 
TrustArc Webinar - Unlock the Power of AI-Driven Data Discovery
TrustArc Webinar - Unlock the Power of AI-Driven Data DiscoveryTrustArc Webinar - Unlock the Power of AI-Driven Data Discovery
TrustArc Webinar - Unlock the Power of AI-Driven Data Discovery
 
AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)
AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)
AI+A11Y 11MAY2024 HYDERBAD GAAD 2024 - HelloA11Y (11 May 2024)
 
Strategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
Strategize a Smooth Tenant-to-tenant Migration and Copilot TakeoffStrategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
Strategize a Smooth Tenant-to-tenant Migration and Copilot Takeoff
 
Artificial Intelligence Chap.5 : Uncertainty
Artificial Intelligence Chap.5 : UncertaintyArtificial Intelligence Chap.5 : Uncertainty
Artificial Intelligence Chap.5 : Uncertainty
 
Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...
Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...
Apidays New York 2024 - APIs in 2030: The Risk of Technological Sleepwalk by ...
 
Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...
Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...
Web Form Automation for Bonterra Impact Management (fka Social Solutions Apri...
 
presentation ICT roal in 21st century education
presentation ICT roal in 21st century educationpresentation ICT roal in 21st century education
presentation ICT roal in 21st century education
 
Finding Java's Hidden Performance Traps @ DevoxxUK 2024
Finding Java's Hidden Performance Traps @ DevoxxUK 2024Finding Java's Hidden Performance Traps @ DevoxxUK 2024
Finding Java's Hidden Performance Traps @ DevoxxUK 2024
 
JohnPollard-hybrid-app-RailsConf2024.pptx
JohnPollard-hybrid-app-RailsConf2024.pptxJohnPollard-hybrid-app-RailsConf2024.pptx
JohnPollard-hybrid-app-RailsConf2024.pptx
 
"I see eyes in my soup": How Delivery Hero implemented the safety system for ...
"I see eyes in my soup": How Delivery Hero implemented the safety system for ..."I see eyes in my soup": How Delivery Hero implemented the safety system for ...
"I see eyes in my soup": How Delivery Hero implemented the safety system for ...
 
AWS Community Day CPH - Three problems of Terraform
AWS Community Day CPH - Three problems of TerraformAWS Community Day CPH - Three problems of Terraform
AWS Community Day CPH - Three problems of Terraform
 
Introduction to Multilingual Retrieval Augmented Generation (RAG)
Introduction to Multilingual Retrieval Augmented Generation (RAG)Introduction to Multilingual Retrieval Augmented Generation (RAG)
Introduction to Multilingual Retrieval Augmented Generation (RAG)
 
Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024
Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024
Modular Monolith - a Practical Alternative to Microservices @ Devoxx UK 2024
 
ICT role in 21st century education and its challenges
ICT role in 21st century education and its challengesICT role in 21st century education and its challenges
ICT role in 21st century education and its challenges
 
How to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerHow to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected Worker
 
FWD Group - Insurer Innovation Award 2024
FWD Group - Insurer Innovation Award 2024FWD Group - Insurer Innovation Award 2024
FWD Group - Insurer Innovation Award 2024
 
Six Myths about Ontologies: The Basics of Formal Ontology
Six Myths about Ontologies: The Basics of Formal OntologySix Myths about Ontologies: The Basics of Formal Ontology
Six Myths about Ontologies: The Basics of Formal Ontology
 
Cloud Frontiers: A Deep Dive into Serverless Spatial Data and FME
Cloud Frontiers:  A Deep Dive into Serverless Spatial Data and FMECloud Frontiers:  A Deep Dive into Serverless Spatial Data and FME
Cloud Frontiers: A Deep Dive into Serverless Spatial Data and FME
 
Introduction to use of FHIR Documents in ABDM
Introduction to use of FHIR Documents in ABDMIntroduction to use of FHIR Documents in ABDM
Introduction to use of FHIR Documents in ABDM
 

Pglo sds

  • 1. Iván García<br />PGlo SDS Page:<br />SDS page was basically an extension of the overall pGlo experiment. This SDS page was conducted on proteins using the electrophoresis technique. The bacteria used in the previous experiment were used as protein samples in our SDS page. An SDS Page uses an agar gel, which is then loaded with the samples and in each corner is loaded with ladders to serve as controls. Basically the electrophoresis drags down the denatured protein depending on their weight; the further down they travel the lighter they are. This workshop was provided by Dr. Chiesa and was useful in determining which proteins contained GFP and which didn’t express the GFP gene. <br />