Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Bacterial genetics

1,456 views

Published on

Bacterial genetics

Published in: Education, Technology
  • Be the first to comment

Bacterial genetics

  1. 1. BACTERIAL GENETICS <ul><li>Submitted To :- </li></ul><ul><li>DEPT OF VETERINARY MICROBIOLOGY </li></ul><ul><li>SUBMITTED BY :- </li></ul><ul><li>ALOK BHARATI </li></ul><ul><li>RVM/08-32 </li></ul><ul><li>DEPT OF AGB </li></ul>
  2. 2. CENTAL DOGMA
  3. 3. RECOMBINATION
  4. 5. GENETIC ELEMENTS <ul><li>Plasmids </li></ul><ul><li>Bacteriophage </li></ul><ul><li>Transposons </li></ul>
  5. 6. GENETIC TRANSFER <ul><li>Conjugation </li></ul><ul><li>Transduction </li></ul><ul><li>Transformation </li></ul>
  6. 7. CONJUGATION <ul><li>Bacterial &quot;sex&quot; or mating </li></ul><ul><li>Exchange of genetic information through a hollow tube, the pilus, from the F+ donor cell to the F- recipient bacterial cell </li></ul>
  7. 9. TRANSDUCTION
  8. 11. TRANSFORMATION <ul><li>Natural - Streptococcus pneumoniae expresses DNA-binding proteins on the cell surface when in stationary phase growth conditions. This natural competent state allows uptake of &quot;naked DNA&quot; </li></ul><ul><li>In vitro (Ca 2+ heat shock, PEG, electroporation); artificially induced competence due to increased membrane permeability </li></ul>
  9. 13. MUTATION <ul><li>CAUSES : </li></ul><ul><ul><ul><li>Spontaneous -point (error) - deletion - insertional sequence element (IS) </li></ul></ul></ul><ul><ul><ul><li>Induced (mutagen) - indirect (requires DNA replication) - direct (affects transcription or translation) - repair-induced (error prone) </li></ul></ul></ul>
  10. 16. REPAIR <ul><li>Direct Repair - enzymatic removal of damages like pyrimidine dimers and alkylated bases </li></ul><ul><li>Excision Repair is removal of damaged DNA segments followed by synthesis of a new DNA strand </li></ul><ul><li>Postreplication Repair is the retrieval of missing information by genetic recombination when both DNA strands are damaged </li></ul><ul><li>Error-Prone Polymerase - induced as a &quot;last resort&quot; before bacterial cell death </li></ul>
  11. 17. GENETIC ENGINEERING
  12. 18. GENETIC CONTROL OVER METABOLIC FUNCTION The Operon Repressor Regulation Inducible Regulation
  13. 19. THANK YOU

×