flow injection analysis is used in analytical chemistry to analyse inorganic ions and samples

1. Automation and Flow
Injection analysis
NAME - APURVA
ARUN JADHAV
CLASS- MSC-PART-1
SUBJECT-CHEMISTRY
ROLL.NO-8043

2. What is automation?
 Automation is the application of technology to carry out operations without
human assistance
 In context of analytical chemistry automation may involve operations like the
preparation of samples, the measurement of responses and calculation of results

3. Unit operations

4. Advantages of Automated Analysers
 Automated instruments save labor costs.
 Their speed, which is frequently significantly greater than that of manual devices.
 A well-designed analyzer can usually produce more reproducible results over a
long period of time than can an operator employing a manual instrument.

5. Types of automatic systems
 Continuous flow analysers- in continuous flow analyser the sample becomes part
of a flowing stream where several of the steps take place as the sample is carried
from the injection point to flow-through measuring unit and then to waste
 Discrete analysers- individual samples are maintained as separate entities and
kept in separate vessels throughout each unit operations

6. Flow injection
analysis
 It is a type of continuous
flow analysis
 In FIA a fluid(liquid or
gas) sample is injected
into a continuously
flowing liquid carrier that
contains a reagent that
reacts with the sample

8. Sample and reagent transport system
 Peristaltic pump is used to pump solution through a flexible tubing.
 Modern pumps have 8 to 10 rollers
 The flow rate is controlled by the speed of the motor
 Which is greater than 30rpm

9. Diagram of peristaltic pump

10. SAMPLE INJECTORS AND DETECTORS
 MOST USEFUL AND CONVENIENT INJECTORS ARE SAMPLING LOOPS

12. SEPERATIONS IN FIA
 DIALYSIS
DIALYSIS IS USED TO SEPARATE INORGANIC IONS SUCH AS CHLORIDE OR SODIUM
IONS

13. DIALYSIS FLOW MODULE

14. GAS DIFFUSION
GAS DIFFUSION FROM A DONOR STREAM CONTAINING GASEOUS ANALYTE TO AN
ACCEPTOR STREAM CONTAINING REAGENT THAT PERMITS ITS DETECTION
IT IS A HIGHLY SELECTIVE TECHNIQUE
THE MODULE IS SIMILAR TO THAT OF DIALYSIS FLOW MODULE
MEMBRANE IS HYDROPHOBIC MADE UP OF POROUS MATERIAL SUCH AS TEFLON
OR ISOTACTIC POLYPROPYLENE

15. PRINCIPLES
OF FIA
 SAMPLE BAND
 REACTION OCCURS AT
THE LEADING AND
TRAILING EDGES OF THE
SAMPLE BAND
 THE REACTION OCCURS
IN MIXING COIL WHERE
THE REAGENT AND THE
SAMPLE REACT TO FORM
A PRODUCT

16.  FORMATION OF PRODUCT DEPENDS UPON THE
 1) RATE OF MIXING
 2) RECTION RATE
 TIME BETWEEN INJECTION AND DETECTION IS TYPICALLY UNDER A MINUTE

17. QUESTIONS CAN BE ASKED ON
 INSTRUMENTATION OF FIA
 SCHEMATIC DIAGRAM OF FIA
 PRINCIPLE OF FIA
 APPLICATION OF FIA

18. REFERENCES
 PRINCIPLES OF INSTRUMENTAL ANALYSIS – BY SKOOG,HOLLER,CROUCH 5TH
EDITION
 GOOGLE IMAGES