The steps of a Western blot are: separate proteins by electrophoresis, transfer proteins from the gel to a membrane, add antibodies specific to the protein of interest and antibodies with an attached enzyme, then add substrate to produce a colored product.
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Put steps of the Westem blot in order Drag and drop options into corre.docx
1. Put steps of the Westem blot in order Drag and drop options into correct order and submit. For
keyboard navigation. SHOW MORE add substrate for enzyme. add mouse antibody specific to
protein of interest add antibodies specific to mouse antibodies that have enzyme attached transfer
proteins from gel to membrane with electrical current separation of antigen solution by
electrophoresis I look for colored product