Presented by: Andrea Valentina Patiño buendia

1. Andrea Valentina Patiño Buendia
Third semester
Universidad Pontificia Bolivariana
2022

2. FAD
It’s in a flavin prosthetic
group utilized as the
redox center of various
proteins.
Introduction
Cryptochrome
Cryptochromes are
photoreceptors that
regulate entrainment by
light of the circadian
clock in plants and
animals.

3. What do this
evaluate?
This study analyzes: A plant-like
cryptochrome of diatom
microalgae, CryP, FAD neutral
radical, photolyase homology
region, photoreceptor, signal
transduction and C-terminal
extension of CryP

4. Objetive
Unstructured C-terminal extension (CTE)
of a plant-like cryptochrome of diatom
microalgae, CryP, that has an influence on
the redox state of the flavine.

5. Methods
Immunodetection (immunological
detection) is used to identify
specific proteins blotted to
membranes.
Is a widely used method for the analysis
of complex protein mixture extracted
from cells, tissues, or other biological
samples. Is used in 2 sequential steps:
Isoelectric focusin and SD-PAGE-
Immunodetection of proteins 2D PAGE

6. Methods
Spectroscopy is the technique of
splitting light (or more precisely
electromagnetic radiation) into its
constituent wavelengths (a
spectrum).
It is a technique used for the
separation os DNA, RNA or protein
molecules according their size and
electrical charge using an electric
current applied to a gel matrix
Spectroscopy Electrophoretic shift assay

7. Results

8. Results

9. Discussion
Author Contribution Agreement
Liu et. al In contrast to the strong influence on the redox state of
the FAD, the CTE had no influence on the ability of CryP to
form dimers. Whereas for higher plant CRYs light-
dependent oligomerisation was proven (Liu et al., 2020),
CryP seems to dimerize independent of the redox state of
its flavin via electrostatic interactions.
Franz-Badur et. al In green algal CraCRY, however, it was recently
demonstrated that the CTE is already connected to the
PHR in the dark state, but changes its conformation upon
reduction of the flavin
Juhas et. al; Konig
et. al
CryP was described as a photoreceptor of P. tricornutum,
containing FAD and MTHF as chromophores and
influencing protein expression and mRNA levels under BL
illumination as used here

10. 1. As more studies are related, we can note the importance of molecular
biology in the field of medicine, which was not sufficiently assessed.
It has made those advances in our daily lives possible to achieve
them by providing some ease and effectiveness with the research
methods it provides.
1. In relation to the study, we see how an organism can be so important
in analyzing data related to a topic that today has taken the focus on
science and research, DNA and how it can be modified. With small
studies, the greatest discoveries have been reached over time.
Conclusions

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Thanks
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12. Conceptual map