This is synopsis for thesis on identification of dermatophytes in clinically suspected cases. For post graduate residents of Microbiology Department. It consists of title introduction aim objectives rationale methodology prcedure planned statistical analysis plan master charts tables references.

1. MAHATMA GANDHI MEMORIAL MEDICAL
COLLEGE, INDORE
Synopsis
Thesis for M.D. (Microbiology)
“CROSS SECTIONAL STUDY FOR ISOLATION AND IDENTIFICATION OF
FUNGAL SPECIES FROM ALL CLINICALLY SUSPECTED CASES OF
DERMATOPHYTOSIS IN TERTIARY CARE HOSPITAL OF CENTRAL INDIA”
Principle Investigator- Dr . Ananya Verma
Guide:
Dr. Suneel Kumar Ahirwar
Associate Professor
Microbiology Department
Co-Guide:
Dr. Anju Mahor
Assistant Professor
Microbiology Department
PG Resident, Batch 2021
Department of Microbiology
M.G.M Medical College, Indore

2. INDEX
1. TITLE
2. INTRODUCTION AND RATIONALE
3. AIM AND OBEJECTIVE
4. METHODOLOGY
STUDY CENTER
STUDY DESIGN
STUDY DURATION
5. SAMPLE SIZE
6. INCLUSION AND EXCLUSION CRITERIA
7. STATISTICAL METHOD / STUDY ANALYSIS
8. SOURCE OF FUNDING
9. MASTER CHARTS AND DUMMY TABLES
10. REFERENCES

3. TITLE
“CROSS SECTIONAL STUDY FOR ISOLATION AND IDENTIFICATION OF
FUNGAL SPECIES FROM ALL CLINICALLY SUSPECTED CASES OF
DERMATOPHYTOSIS IN TERTIARY CARE HOSPITAL OF CENTRAL INDIA”

4. Introduction
• Dermatophytes are the most common cause of superficial cutaneous fungal
infections of human body mainly involving the keratinised tissues like skin ,hair
and nails .
• It includes three genera-
● Epidermophyton
● Microsporum
● Trichophyton
They are collectively known as Dermatophytes.
● The tinea infections are prevalent globally, but they are common in tropics and in
geographical areas with higher humidity , overpopulation, and poor hygienic living
conditions.
● An increasing frequency of Dermatophytosis has been observed during last two
decades especially in immunocompromised patients such as AIDS, Diabetes
mellitus, cancer and organ transplantation patients.

5. • There has been a significant increase in the incidence of chronic, relapsing
and recurrent cases of superficial Dermatophytosis in India that are often
unresponsive to conventional drugs and doses of recommended antifungal
treatment.
According to WHO, the prevalence rate of superficial mycotic infections
worldwide has been found to be 20-25%.
The recent prevalence of Dermatophytosis in India ranges from 36.6-78.4%.

6. RATIONALE OF THE STUDY
● This study is being conducted for the first time at MGM Medical College, Indore .
● This study will reveal most common fungal species causing Dermatophytosis in
central India .
● It will also help clinicians to start antifungal treatment as early as possible.

7. AIM AND OBJECTIVE
● To Identify the causative fungal species from all clinically suspected cases of
Dermatophytosis in a tertiary care hospital of Central India .

8. METHODOLOGY
Study centre: Mahatma Gandhi Memorial Medical College and
M.Y. Hospital, Indore
Duration of Study: 1 year after approval of study by institutional
scientific and Ethical Committee.
Study design-Cross sectional study

9. Sample: All clinically suspected cases of Dermatophytosis from out
patient department of Dermatology.
Inclusion criteria: All age groups of either sex with clinical features
of Dermatophytosis.
Exclusion Criteria: Nil
Sample size: Minimum 400 samples

10. Materials and Methods
● Sample taken from the infected areas like skin , nail and hair of patients of all age
group from out patient department of Dermatology.

11. Procedure Planned
Sample received from out patient department of dermatology [like skin scrapping, nail clipping, hair]
Direct Microscopic Examination
(10-20%, or 40% KOH Mount)
Fungal culture
(SDA Media and DTM) Incubate at 25 ̊̊C and 37 ̊̊C for 4 weeks and will be observed at regular intervals.
Growth
Lactophenol Cotton Blue Mount
Slide Culture
Identification of Species

12. Statistical Analysis Plan
• Data will be entered in excel sheet and will be analysed using open sources
software.
• Continuous data will be expressed in terms of mean and standard deviation.
• Categorical data will be expressed in proportion and percentage.
• Appropriate test of significance will be applied wherever necessary and p-value
<0.05 will considered as statistically significant.
Source of Funding: None

13. Master Chart
Serial
No.
Lab No. OPD No. Age Sex Sample Clinical diagnosis Fungal
isolates
Species
1.
2.
3.
4.
5.

14. DUMMY TABLES
Table 1: Demographic Profile of Dermatophytosis
SERIAL NO. AGE MALE(%) FEMALE(%) TOTAL(n)
1. 1-10 YEARS
2. 11-20 YEARS
3. 21-30 YEARS
4. 31-40 YEARS
5. 41-50 YEARS
6. 51-60 YEARS
7. >60 YEARS

15. TABLE 2: Fungal Species Isolates
SERIAL NO. FUNGAL
SPECIES
SKIN HAIR NAILS TOTAL NO.
OF
ISOLATES
1.
2.
3.
4.

16. References:
• 1.Chandra j. Textbook of medical mycology. New Delhi. Jaypee
Publishers;2018;4:179-182
• 2.Havlickova B, Czaika VA , Epidemiology trends in skin mycosis worldwide.
Mycosis 2008;4:2-15
• 3.kannan P,Janaki, Selvi GS Prevalence of dermatophytes and other fungal agents
isolated from clinical samples. Indian j. Med microbiology. 2006;24:212-5.
• 4.Uthansingh K, Sahu , Debata NK. Isolation and identification of fungus
associated with skin and nail scalps. Apollo Med. 2019;16:16-21
• 5.Rajagopalan et al. BMC Dermatology;2018:18:6.

17. THANK YOU….
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