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Plant tissue culture A Presentation ByMr. Allah dad KhanVisiting Professor the University of Agriculture Peshawar allahdad52@gmail.com
1.
2. Plant Tissue CulturePlant Tissue Culture
A Presentation ByA Presentation By
Mr. Allah dad KhanMr. Allah dad Khan
Visiting Professor the University ofVisiting Professor the University of
Agriculture PeshawarAgriculture Peshawar
allahdad52@gmail.comallahdad52@gmail.com
3. What is Plant Tissue Culture?What is Plant Tissue Culture?
Plant tissue culture is a form of asexual
propagation of plants under laboratory
conditions.
4. Why is Plant Tissue Culture Important?Why is Plant Tissue Culture Important?
It has made significant contributions in:
◦ The production of plant material
◦ Plant breeding
◦ Gene banks
◦ The production of chemical compounds
5. How is Plant Tissue Culture Done?How is Plant Tissue Culture Done?
It is grown on a special culture medium
which supports its growth and
development.
The medium can be either semisolid,
such as agar, or liquid, such as purified
water.
6. Microorganisms and Plant Tissue CultureMicroorganisms and Plant Tissue Culture
In the practice of plant tissue culture,
microorganisms are called “contaminants”
because of their harmful effects on plant growth
in vitro.
Six potential sources of contamination in the
plant tissue culture lab are:
◦ Air
◦ Water
◦ Growth Media
◦ People
◦ Equipment
◦ Plant Material
7. Experiment One: DescriptionExperiment One: Description
A one dollar bill was cut in half and
placed in two different petri dishes.
Dollar 1 was placed in a room at 100 F.
Dollar 2 was placed in a room at room
temperature.
15. Day 2: Dollar 2Day 2: Dollar 2
Bacteria begin to
develop in the middle
of the dollar; although
development is not as
rapid as in Dollar 1.
16. Day 3: Dollar 2Day 3: Dollar 2
The bacteria have
spread to the edges of
the dollar.
17. Day 6: Dollar 2Day 6: Dollar 2
The bacteria cover
the majority of the
bill.
18. Day 7: Dollar 2Day 7: Dollar 2
The entire dollar is
covered with bacteria.
19. ResultsResults
The bacteria on dollar 1 developed at a
more rapid pace; however, the agar
dissolved due to the high temperatures.
The bacteria on dollar 2 developed at a
slower pace in the beginning of the
experiment, but dollar 2 contained more
bacteria than dollar 1 at the conclusion.
20. Experiment 2: DescriptionExperiment 2: Description
A throat culture was taken from two
different people at the same time.
Mouth 1 was taken from a person with
flu-like symptoms.
Mouth 2 was taken from a healthy
person.
22. Day 1: Mouth 1Day 1: Mouth 1
The bacteria have
grown slightly and
exhibit a faint
yellowish color.
23. Day 2: Mouth 1Day 2: Mouth 1
The bacteria have
grown considerably
and can be seen with
the naked eye.
24. Day 3: Mouth 1Day 3: Mouth 1
The bacteria are
spreading.
25. Day 6: Mouth 1Day 6: Mouth 1
The bacteria have
grown considerably:
yellowish colored
spots are beginning to
form.
26. Day 7: Mouth 1Day 7: Mouth 1
The bacteria are still
growing at a rapid
pace and can now be
seen easily without a
microscope.
27. Day 1: Mouth 2Day 1: Mouth 2
Very little bacteria
have formed.
28. Day 2: Mouth 2Day 2: Mouth 2
The bacteria begin to
grow but cannot be
seen without a
microscope.
29. Day 3: Mouth 2Day 3: Mouth 2
The bacteria are now
growing rapidly, and
small yellowish spots
slightly visible to the
naked eye are
beginning to form.
30. Day 6: Mouth 2Day 6: Mouth 2
The bacteria have
grown gradually, and
are easier to see with
the naked eye.
31. Day 7: Mouth 2Day 7: Mouth 2
The bacteria are
growing slowly, but
the small, yellowish
spots appear clearly.
32. ResultsResults
The mouth 1 culture grew a larger
amount of bacteria at a faster rate than
the mouth 2 culture.
Mouth 2, however, did begin to grow
towards the end of the experiment.