Plant tissue culture A Presentation ByMr. Allah dad KhanVisiting Professor the University of Agriculture Peshawar allahdad52@gmail.com

2. Plant Tissue CulturePlant Tissue Culture
A Presentation ByA Presentation By
Mr. Allah dad KhanMr. Allah dad Khan
Visiting Professor the University ofVisiting Professor the University of
Agriculture PeshawarAgriculture Peshawar
allahdad52@gmail.comallahdad52@gmail.com

3. What is Plant Tissue Culture?What is Plant Tissue Culture?
Plant tissue culture is a form of asexual
propagation of plants under laboratory
conditions.

4. Why is Plant Tissue Culture Important?Why is Plant Tissue Culture Important?
It has made significant contributions in:
◦ The production of plant material
◦ Plant breeding
◦ Gene banks
◦ The production of chemical compounds

5. How is Plant Tissue Culture Done?How is Plant Tissue Culture Done?
It is grown on a special culture medium
which supports its growth and
development.
The medium can be either semisolid,
such as agar, or liquid, such as purified
water.

6. Microorganisms and Plant Tissue CultureMicroorganisms and Plant Tissue Culture
In the practice of plant tissue culture,
microorganisms are called “contaminants”
because of their harmful effects on plant growth
in vitro.
Six potential sources of contamination in the
plant tissue culture lab are:
◦ Air
◦ Water
◦ Growth Media
◦ People
◦ Equipment
◦ Plant Material

7. Experiment One: DescriptionExperiment One: Description
A one dollar bill was cut in half and
placed in two different petri dishes.
Dollar 1 was placed in a room at 100 F.
Dollar 2 was placed in a room at room
temperature.

8. Experiment One: resultsExperiment One: results

9. Day 1: Dollar 1Day 1: Dollar 1
No visible change.

10. Day 2: Dollar 1Day 2: Dollar 1
Bacteria begin to
develop around the
edges of the dish.

11. Day 3: Dollar 1Day 3: Dollar 1
Bacteria develop
rapidly covering most
of the dish.

12. Day 6: Dollar 1Day 6: Dollar 1
Bacteria cover the
entire dollar and dish.

13. Day 7: Dollar 1Day 7: Dollar 1
The bacteria continue
to spread, and the
nutrient agar has
begun to dissolve.

14. Day 1: Dollar 2Day 1: Dollar 2
No visible change.

15. Day 2: Dollar 2Day 2: Dollar 2
Bacteria begin to
develop in the middle
of the dollar; although
development is not as
rapid as in Dollar 1.

16. Day 3: Dollar 2Day 3: Dollar 2
The bacteria have
spread to the edges of
the dollar.

17. Day 6: Dollar 2Day 6: Dollar 2
The bacteria cover
the majority of the
bill.

18. Day 7: Dollar 2Day 7: Dollar 2
The entire dollar is
covered with bacteria.

19. ResultsResults
The bacteria on dollar 1 developed at a
more rapid pace; however, the agar
dissolved due to the high temperatures.
The bacteria on dollar 2 developed at a
slower pace in the beginning of the
experiment, but dollar 2 contained more
bacteria than dollar 1 at the conclusion.

20. Experiment 2: DescriptionExperiment 2: Description
A throat culture was taken from two
different people at the same time.
Mouth 1 was taken from a person with
flu-like symptoms.
Mouth 2 was taken from a healthy
person.

21. Experiment two: resultsExperiment two: results

22. Day 1: Mouth 1Day 1: Mouth 1
The bacteria have
grown slightly and
exhibit a faint
yellowish color.

23. Day 2: Mouth 1Day 2: Mouth 1
The bacteria have
grown considerably
and can be seen with
the naked eye.

24. Day 3: Mouth 1Day 3: Mouth 1
The bacteria are
spreading.

25. Day 6: Mouth 1Day 6: Mouth 1
The bacteria have
grown considerably:
yellowish colored
spots are beginning to
form.

26. Day 7: Mouth 1Day 7: Mouth 1
The bacteria are still
growing at a rapid
pace and can now be
seen easily without a
microscope.

27. Day 1: Mouth 2Day 1: Mouth 2
Very little bacteria
have formed.

28. Day 2: Mouth 2Day 2: Mouth 2
The bacteria begin to
grow but cannot be
seen without a
microscope.

29. Day 3: Mouth 2Day 3: Mouth 2
The bacteria are now
growing rapidly, and
small yellowish spots
slightly visible to the
naked eye are
beginning to form.

30. Day 6: Mouth 2Day 6: Mouth 2
The bacteria have
grown gradually, and
are easier to see with
the naked eye.

31. Day 7: Mouth 2Day 7: Mouth 2
The bacteria are
growing slowly, but
the small, yellowish
spots appear clearly.

32. ResultsResults
The mouth 1 culture grew a larger
amount of bacteria at a faster rate than
the mouth 2 culture.
Mouth 2, however, did begin to grow
towards the end of the experiment.