Scope Of Microbiology

Scope of Microbiology
SCOPE OF MICROBIOLOGY: What is Microbiology?| пїЅ Microbiology is the study of microorganismsпїЅ Microorganisms, roughly, are living
things that are too small to be seen with the naked eyeпїЅ Microorganisms cannot be distinguished phylogenetically from пїЅmacroorganismsпїЅ (see
variety of microorganisms, below)пїЅ Microbiology is more a collection of techniques: aseptic technique, pure culture technique, microscopic
observation of whole organisms, etc.пїЅ Microbiologists isolate specific, culturable microorganisms from wild populations, then study them (or,
alternatively, study them in situwithout culturing them)| [Microbiology is the study of organisms too small to be seen with the naked eye. Originally,
...show more content...
| helminths:| Flatworms (platyhelminths), roundworms (nematodes)| metazoan (multicellular animal) parasites, engulfers and absorbers|
chemohetero–| –––| tape worm, trichinosis, hook worm, etc.| protozoa:| Unicellular and slime molds, flagellates, ciliates| eucaryotes, parasites, engulfers
and absorbers, wet conditions, no cell wall, ~30 human pathogens| chemohetero–| cysts (some)| malaria, giardiasis, amoebic dysentery, etc.| viruses:|
Enveloped, non–enveloped| acellular, obligate intracellular parasites| not applicable| virion particles, encased in durable state of host| common cold, flu,
HIV, herpes, chicken pox, etc.|
Micro organisms are useful to us in many ways. For example, * Bacterium Lactobacillus convert milk into curd. * Bacteria are also involved in the
making of cheese. * Acetobacter aceti is used for production of acetic acid from alcohol. * Yeast is used for commercial production of alcohol and
wine. * Antibiotics are manufactured by growing specific microorganisms. * Some bacteria fix atmospheric nitrogen and increase the soil fertility. *
Bacteria are used in the preparation of medicines. * Bacteria are used in the formation of pickles and many other food items. * Yeast is also used in
baking industry for making breads,
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Essay about Microbiology Unknown Bacteria
INTRODUCTIONS:
Microorganisms are both beneficial and harmful. These microorganisms are important to humans because they play a role in the ecology of life, by
decomposing wastes, both natural and man–made, such as creating nitrogen fertilizer at the root zones of certain crops. Other several pathogens that
can cause serious harm, even immediate death due to the diseases or disease causing products they produce. Overall, microorganisms play an important
role in life.
The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the
microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible...show more content...
Then the following differential tests were performed:
Gram Stain Test:
Two smears of the unknown bacterium #5 were inoculated while the second smear was used for a back up. The unknown bacterium dried for at least
forty minutes. After the smears dried, the slides were heat fixed two times to ensure the stability of the organism. The slide was placed on top of the
staining rack then over the small sink.
The drops of crystal violets, approximately 15 drops, were flooded until the smear were all covered and then allowing resting for one to two minutes.
After two minutes, the slide was titled over the sink and washed off, with the distilled water bottle, by aiming the stream of water above the smear. The
specimen appeared blue–violet when observed with the naked eye. The drops of Gram's iodine were applied on the slide until covered and then
allowed to react for one minute or more. After the time elapsed, the slide was rinsed again with distilled water following immediate drops of Gram
stain decolorizer added one drop at a time.
Again, after ten seconds, the slide was rinsed with distilled water to wash off the decolorizer. The slide was placed back on the staining rack.
Approximately ten drops of safranin were covered on the slide allowing resting for one minute. After one minute, the slide was rinsed one final time
on the small sink followed by blotting the stained slide with Bibulous paper. The slide was placed between pages of Bibulous paper to help

Statement Of Purpose For Microbiology
Statement of Purpose
Name: Ujjwal Rimal
Area of Interest: Microbiology and Immunology.
During his last few months, my grandfather had trouble falling asleep. Frequent cough and his debilitating health was the matter of concern for
our family. The nights had always been horrible as my father had to take care of him till the dawn. At that time, lung cancer was a fatal disease in
Nepal. His demise gave me an insight into how illness can affect a person's life and of the people close to him. Intrigued by knowing that a simple
microscopic cell with an uncontrolled division took my dearest one far from me, I began to ponder over the enigma of a microbiological world. As I
grew, my curiosity and inquisitiveness towards diseases began to escalate. Thus, I pursued my growing interest by opting forBiology as one of the
majors in high school.
After completion of Intermediate in Science with biology, I decided to pursue Microbiology as my major in undergraduate studies at Tri–Chandra
Multiple Campus...show more content...
These have forced me to look for other opportunities to continue my education. Department of Biology at the University of Louisiana at Lafayette has
excellent facilities and leading laboratories in which I can fully realize my scientific interests. I would regard my admission to the University of
Louisiana at Lafayette not only as a great honor but also as a great responsibility and an obligation to work hard. Furthermore, I chose your country
because it is the most developed country in the world which has the ability to provide all types of education to the domestic as well as the international
student. It has a quality educational institution for better education. Besides these, a lot of foreign students come to the USA to pursue their higher
education, which provides an opportunity to meet new peoples and share knowledge, experience, as well as a cultural

Microbiology Lab Report
Lab Report
A differential staining procedure used in microbiology is called the Gram stain. The significance of the Gram staining procedure is that it differentiates
the bacteria into two groups. The Gram reaction of a bacterium adds valuable information for the treatment of disease. The Endospore stain is a special
stain that is used to determine the presence of endospores in bacteria. This stain is used to detect if certain bacterium cell contain highly opposing spores
within their vegetative cells. The acid–fast staining technique differentiates bacteria into distinctive groups. The importance of the technique is that it
can identify all bacteria in the genus Mycobacterium. Two pathogens are included in this genus, Mycobacterium tuberculosis and Mycobacterium
leprae. In order to start a stain, the microorganisms must be fixed to the microscope slide. This first step is called preparing a smear. Spread the
specimen on a slide and let it air–dry. Once the specimen is dry it must then be heat fixed by waving the slide just one or two times over the flame.
After this step is completed, the staining techniques begin. The Gram stain is considered a differential staining technique because it places bacteria into
categories. The first step during the Gram staining technique is applying the primary stain, crystal violet, for twenty to sixty seconds. After this is
completed, rinse with water and proceed to add the mordant stain, iodine, for one minute. The mordant

Microbiology Personal Statement
I have chosen to pursue a major in microbiology due to my high interest in public and international health. I am interested in how microbes play a role
in the functionality of the human body. Moreover, I find the ecological roles of microbes to be critical to our understanding of other fields of science
that impact human health. The niches of microbes throughout a variety of environments indicates the human impact on our ecosystem.
Service has been important in my life which has inspired me to pursue a minor in non–profit leadership. I witnessed firsthand how non–profits can
positively influence the health of others through working within the pharmacy of Urban Ministries to provide medications for underprivileged citizens,
leading simulating activities within Pruitt Health to increase the quality of treatment of the elderly, and providing nutritious meals within Clemson
Community Care and a locally based organization called With Love from Jesus. The major issues concerning the non–profit sector include
sustainability and the gathering of resources. I have viewed these issues through integrating science and service by assisting with teaching laboratory
bench techniques to high–school...show more content...
subtilis has shaped my certainty in my microbiology related field of study. My participation in Alternative Spring Break trips focused on the
importance of physical and mental health education within women and children in crisis has shaped my certainty in my field of study. Microbiology
is broad, providing many applications for its use in the treatment of mental illness. Studies have shown that the interaction between the gut microbiota
and the brain could be a factor in the obtaining of mentally related diseases. There are broad applications of microbiology. This field is always
evolving, which allows a wide variety of

Microbiology Essay
One of the most common bacteria known to man is affiliated with the genus Staphylococcus. Staphylococcus aureus can be described as bacteria that
present as gram positive cocci either in pairs, tetrads, short chains or irregular grape like clusters (2A). With their diameter ranging from 0.5 to 1.5 um,
other characteristics that comprise this bacteria include their non–spore forming ability and nonmotile ways (2A). Defined as facultative anaerobes,
these microorganisms are usually encapsulated(2A) and are a member of the normal flora that resides on humans(2D). In humans, a common site of
habitation would be the nares(2D). The chances of S.aureus colonization are greater in individuals that are diagnosed with hiv, insulin–dependant...show
more content...
An example would be when individuals who are asymptomatic nasal carriers of this bacteria accidentally autoinoculate another part of their body,
resulting in them now being infected (DIC). An usual route that can also be taken to assist in the spread of S.aureus is through fomites(3a). Though
highly unlikely, sharing towels was once the cause of staphylococcal infections in a high school football team(3a). The different opportunities presented
for the dissemination of the microorganism are the factors contributing to its transportation throughout communities.
To increase their effectiveness, many organisms resort to producing molecules called virulence factors. These molecules prove to be beneficial to the
pathogen by strengthening their pathogenic capabilities. Through many examinations and dissections performed on S.aureus, it was revealed that that
bacteria itself was home to a wide range of virulence factors. Being the most common, the pathogen is comprised of surface adhesions whose main job
is to regulate the attachment of the bacteria to its target tissues(4b). This in turn helps guarantee the bacteria a better chance of invading the tissue and
causing an infection. In addition, another important factor is the polysaccharide capsule(4c). Even though many variations of the capsule have been
reported, type 5 and 8 have been deemed to play a more

Microbiology Questions
Me: Question #1– How can you briefly describe what a microbiologist is to those who do not know?
Metzler: Someone who studies small things, like bacteria or viruses, and tries to figure out how they function, how they interact with organisms, and
how organisms try to fight back.
Me: Question #2– What grabbed your interest to pursue your studies in microbiology?
Metzler: It really started when I was a freshman in college. The immunologist at my school came and spoke to my introductory to biology class and
was talking to use about the immune system and was discussing the randomness of how antibodies are created and was saying that we can have
antibodies in our body that could fight something that exists in outer space or who knows what because it is all done randomly. I just thought that was
absolutely fascinating and that's when I really got interested in immunology, which is a part of microbiology. It's all related.
Me: Question #3– Did you want to be a microbiologist/scientist as a child. If not, what did you want to be?
Metzler: No, I did not. As a child, I wanted to be a doctor. When growing up, I really did not know about scientists or people with PhDs or anything
like that. I always had an interest in science in school and I did really well in it and my school was like oh you did really good in this and you...show
more content...
I guess probably knowing that we don't need to be so scared and petrified about bacteria. I think knowledge is power and most bacteria are completely
harmless to us. A lot of bacteria are quite beneficial to us. There are just a few among the big giant group that can actually make you sick. Even if
they do make you sick, I think if you understand how they are doing that so that you won't blow it out of portion. So I think having that knowledge
makes me calmer about getting sick and calmer when my kids get sick or anything like that because I know necessary it not as horrible as it is if you
didn't have that

Microbiology Test Questions
Week 4: Microbiology Short Answer Questions
Infections and Methods of Microbial Control
1. Explain some of the problems involved in sterilizing delicate and heat–sensitive instruments such as endoscopes, catheters, etc.
Instruments and equipment such as endoscope and catheter involves invasive procedure that would be exposed to body fluids and contaminants so it
should greatly be a concern for infection control, thereby choosing the right method of sterilization is sensitive. Problem may arise and transmission of
disease may be imminent if not properly sterilized. Disinfection of these reusable instruments poses significant problems. It cannot be autoclaved. It
should be handled by mechanical cleaning, followed by high–level disinfection, rinsing and drying.
2. Perform an online search on the effectiveness of using hand sanitizers versus hand washing. Write a brief summary of what you find. Be sure to
include the URL(s) of your source(s).
Several topics can be read from the Center for disease control...show more content...
It may be a virus or bacteria that would cause an illness. The reservoir is the place where the pathogen may adhere to live and survive. It can be a
person, animal, or environmental component such as water or soil. The portal of exit is the invasion or place where the pathogen may leave the
reservoir. It can be saliva, mucous membranes, feces, blood or any discharges coming out from the body. The means of transmission can be directly
or indirectly transmitted. Direct transmission requires close association with the infected host, but not necessarily physical contact. Indirect contact
requires a vector, such as an insect or animal. The portal of entry can be done in three ways: penetration or a cut to the skin, inhalation, or ingestion.
Then it is already in the new host, which will have various factors on how the host will be affected by its severity and will also depend on the health
and well being of the

Essay On Microbiology
For this analysis assignment, I used the American Type Culture Collection (ATCC) website to find technical documents from the microbiology field. I
chose two whitepapers written by the same author but the papers themselves have vastly different purposes. The first white paper informs the reader of
multi–resistant strains and the need for new solutions to this problem. The second document discuss a new approach to treating multidrug–resistant
infections. This memo will explain the purpose of each document by comparing the intended audience, genre, conventions and the appeals used.
AUDIENCEAs stated above, both documents discuss multidrug–resistant organisms. Despite their similar topic, each has a different primary audience.
The first...show more content...
The second document presents new and relevant findings. Their competitors will look at thus documents to judge what products they should advertise
and where they should be applying their research. Lastly, both documents must pass the judge, ATCC. In order to be published on the website, these
documents had to be accurate, thorough and relevant. GENREThe documents I chose were both white papers. A whitepaper purposes a solution or
sells information of products as solutions. Whitepapers typically contain background information, a solution, conclusion, works cited, visuals and
examples. The first document and the second document had each of these qualities. Both documents had a header containing the title and the logo of
the company. The first document contained an image of Klebsiella pneumoniae and cultures. The second image contained a single photo if one lone
microorganism. Each document follow the conventions of a white paper. Since the first document is slightly like an advertisement, their produce is
mentioned towards the end of the document as a way to keep the interests of readers. Neither paper violated the conventions of a white paper
document and as a result, both documents are easy to understand. They are also very visually appealing and eye catching. These appealing documents
are published online and are easily accessed. Since, these documents are new they are still able

Microbiology Case Study 1
Introduction to Microbiology – Case Study 1
Kristen Alejos
You are working as an emergency room nurse in Topeka when a mother brings in her 8 year old son because of severe, bloody diarrhea. When
questioned, the boy said that it hurts when he goes to the bathroom and his mother said he had vomited in the previous two days. When asked about
their previous movements, the mother and son both mentioned a backyard barbeque that had taken place 6 days earlier. The boy complained that his
mother made him eat a "pink" hamburger that was not thoroughly cooked. A physical examination of the boy revealed nothing out of the ordinary
except dehydration from the diarrhea. Lab tests showed that he had 13,000 white blood cells/microliter of...show more content...
(3 pts)One important virulence factor produced by E. Coli is toxins. Toxins are proteins secreted by the bacteria that are poisonous to the host. In this
case the toxins that are produced by E. Coli, damage the lining of the small intestine and that is what causes the bloody diarrhea. Sources: –Leboffie,
Michael J. Microbiology. Burton E. Pierce. Morton. Englewood, Co. 2012
3. Based on what you know about the selective and differential capabilities of MacConkey agar, would it be possible to obtain similar results from a
stool sample of a health patient? Why or why not? (3 pts)The MacConkey agar is capable of growing E. Coli because it inhibits the growth of
Gram–positive bacteria and E. Coli is a gram–negative bacteria. If we took a stool sample from a healthy patient the selective media would be similar
because E. Coli always grows on a MacConkey agar and E. Coli is always present in the human intestines. However the differential media would not
be similar because of the amounts of E. Coli in the intestines and also whether it is infectious or not. Different kinds of E. Coli have the ability or
inability to ferment lactose which changes the colors of the dyes after the incubation period. Sources: –Leboffie, Michael J. Microbiology. Burton E.
Pierce. Morton. Englewood, Co. 2012
4. How is this organism spread? How can infection be prevented? (3 pts) According to the Mayo Clinic Staff, E.Coli is most easily

Theme I History Of Microbiology
THEME I HISTORY OF MICROBIOLOGY
DEFINITIONS
Microbiology is the science that studies the biology of microorganisms or microbes.
Microorganisms are beings:
В· Small size, which can not be observed with the naked eye, it takes a microscope.
В· Do not have tissue differentiation, are acellular, one–celled, and / or cenocГticos (multicore).
Naturally occurring in mixed populations of cells, where each cell performs its vital functions independently. If we get a pure or axenic culture must
apply a methodology of Microbiology for selecting one type of microorganisms.
Microorganisms are therefore those acellular biological entities, unicellular, multicellular and / or cenocГticos, devoid of tissue organization, for its
small size, beyond human eye and for study, the methodology of pure culture is required. (examination).
HISTORICAL DEVELOPMENT
Although known recently, profits of microorganisms have been used for thousands of years (bread, wine) ago, suffering their infections, and using
prophylactic measures against them (quarantine, sacrifices, etc.)
In the seventeenth century, Antoine van Leeuwenhoek was the first scientist who observed microorganisms. He was a Dutch optician who built the
first quality lenses (x 300). He noted the structure of seeds, red blood cells, sperm, but its maximum discovery was animalcules; yeasts, algae, protozoa
and some bacterial large.

It took a long time until Microbiology as science develops. This happens at the end of the nineteenth

I am writing to express my interest in the Research Specialist position in your laboratory in the Department of Microbiology and Immunology. I feel
my background as a Bachelor of Science in Molecular and Cellular Biology and my extensive lab experience make me an ideal candidate.
During my undergraduate study at the University of Illinois, I worked in a research laboratory for over two years. I completed a senior thesis, earning a
Distinction in Molecular and Cellular Biology. I also collaborated with graduate students, both within and outside of my laboratory, and made
contributions to their projects, which are currently undergoing peer review. Accordingly, I have a firm understanding of the research process, scientific
writing, and the

Microbiology Study Questions Essay
I. INTRODUCTION
Independent Study Questions 1.) What is the fundamental difference between eukaryotic and prokaryotic cells?
Procaryotic cells do not contain a membrane–bound nucleus or any membrane–bound organelles. Eurkaryotic cells contain both a membrane mound
nucleus and membrane–bound organelles (making them far more complex).
2.) List and describe the two domains of prokaryotic organisms. a. Bacteria– Most are a specific shape (cylindrical, spiral, or spherical). Cell wall
contains peptidoglycan. Multiply by binary fission. b. Archaea– Similar in shape, size, and appearance to Bacteria. Multiply by binary fission. Cell wall
does not contain peptidoglycan.
3.) List and describe the four groups...show more content...
Cell wall also contains teichoic acids (negatively charged chains of a common subunit which various sugars attach to; they stick out of peptidoglycan
layer and bind cations such as Mg2+) and lipoteichoic acids.
A Gram–negative cell is pink when Gram–stained. Its cell wall is characterized by a relatively thin layer of peptidoglycan. There is no presence of
teichoic acids. The layer of peptidoglycan is surrounded by an outer membrane; the outer layer of this membrane is lipopolysaccharide.
4.) Describe the shape of a coccus versus a rod.
A coccus is a spherical–shaped bacterial cell while a rod is a rod–shaped bacterial cell (sometimes called a bacillus)
5.) Where is a cell's cytoplasmic membrane relative to the cell wall?
Just proximal to the cell wall
6.) What is the role of the cytoplasmic membrane?
Serves as a selectively permeable barrier between the cell and its external environment (only a few substances are allowed to pass freely). Also
transmits information about the external environment to the inside of the cell.

7.) Describe the structure of the cytoplasmic reticulum.
The structure is a phospholipid bilayer that is embedded with proteins. Phospholipid molecules are arranged in an opposing manner with the
hydrophobic tails facing inward, and their hydrophilic tails facing out away from the other.
8.) What is the fluid mosaic model?
Membrane proteins are not stationary, but instead

Various test performed to identify two bacterium that were unknown, one was plaque that were scrapped from the teeth and the second was an
unknown given. It resulted that the unknown bacterium is Staphylococcus epidermidis. Staphylococcus epidermidis has three drug resistance, and no
secondary metabolites. The plaque scrapped from the teeth had a morphology of bacilli and is gram positive. Staphylococcus is gram positive, it is
catalase positive and oxidase negative. The minimal inhibition concentration of Ethanol extract is 0.001 micro liters for S. epidermidis. The minimal
inhibition concentration for the plaque was at a concentration 0.1 ml of ethanol extract and a concentration of 0.01 ml for distilled water.
Background:
Staphylococcus...show more content...
Oxidase Test: Using a sterilized loop inoculate a loopful ofbacteria into the test square and observe for any change in color. If it turns blue it is
oxidase positive, and if it remains the same color then it is oxidase negative.
Streaking: Using the method of quadrant streak a loopful of bacteria onto one side of the BHI agar plate and sterilize your loop. Then using the
sterilized loop, grab one end of the side where it was previously streaked and slide it across on another side of the agar plate. Then using the
sterilized loop grab the one end of that same side where it was previously streaked and slide it to the other corner of the agar plate. Sterilized your
loop again using the flame and grab the last end of the previously streaked and streak the last corner of the agar plate. This should have four corners the
fourth corner being the most diluent will have the most isolated colony forming.
Dilution series: Use microcentrifuge tubes for the dilutions, if the final dilution is 10x^4 , you will need 5 microcentrifuge. Label the one as the stock,
10X, 10X^2, 10X^3 and 10X^4, then add 1 ml of the diluent X. Add .9 ml of the diluent to each concentration microcentrifuge and
0.1 ml of the substance that will be diluted on to the first microcentrifuge which is the one labeled as 10X, using the micropipette. Then add 0.1 ml of
from the 10X microcentrifuge onto the 10X^2 microcentrifuge and so on until you reach

Importance Of Microbiology
Importance of Microbiology| in Nursing Agriculture Pharmacy
Advertisement
Microbiology is a subject dealing with microbes and related concepts. Microbiology has come a long way since discovery of microbes and is
presently of great help to mankind. It is used in health care, food production, diagnosis, production of alcohol, maintenance of sterility and cleanliness
etc.
Though the subject was initially limited to study of microbes and their characteristics or properties, latter it was explored to see all possible
applications and benefits to man.
Microbiology was a boon to eradicate mass destructive epidemics like rabies, small pox and many other infectious diseases.
The present society is so dependent on it that without it there can no...show more content...
for sterilization of manufactured drugs. Microbes are destroyed by sterilization. in search of new drugs.
IMPORTANCE OF MEDICAL MICROBIOLOGY:
In medicine microbiology is taught to let pupil understand types of microbial diseases; i.e. how diseases are caused by microbes. Their types like
bacterial, viral, fungal etc.
Diagnosis and treatment; Even diagnosis of the disease causing microbe is taught so as to give right drug and combat infection effectively. The
identification of

During my time at the University of Wisconsin– La Crosse, it was there that I not only discovered my passion for microbiology; but it was where I
became aware of my career goal, to be an educator at the university level. While as a teaching assistant for a Fundamentals of Microbiology
laboratory, I established that I wanted to become a professor. I want to become and educator because, I look back at how much of an impact my
professors at the University of Wisconsin– La Crosse and the Medical College of Wisconsin had not only on me but my peers. It was the professors
who encouraged us, taught us the future of our field, and helped us up when we fell down. I think back at all the times these professors helped me
become who I am know, it I can vividly see myself reciprocating that for future students.
During my employment at the Medical College of Wisconsin, I have discovered an untapped enthusiasm for physiology; and I knew I wanted to
incorporate its concepts with my previous infatuation for microbiology. The fact that such minuscule organisms and cells retain complex pathways and
mechanisms,...show more content...
Most of these classes have an intensive lab portion through which I developed competence in a number of fundamental laboratory techniques and
discovered a passion for bench research. Of all the classes I have taken I feel Immunology, Pathogenic Bacteriology, Microbial Genetics, and Human
Anatomy and Physiology laboratories have been the most useful to me when it came to learning about the diversity of the prokaryotic and eukaryotic
world. However, my classroom experience was not limited strictly to microbiology; I have an array of courses stretching from chemistry courses, like
organic chemistry, and basic biology courses, like comparative endocrinology and human physiology to statistics and physics

Microbiology involves the study of microbes and their relevant scientific roles. The purpose of this lab report is to use the methods and techniques
acquired from the microbiology course to identify an unknown bacterium. The assigned culture for this report is Unknown 28. Various laboratory
techniques were performed in this process in order to draw conclusions as to what microorganism the unknown sample may contain.
Before proper tests can be performed, the most important aspect to laboratory procedures are aseptic techniques. These techniques are crucial to ensure
that no contamination occurs during the culturing process (1). Although the exact steps may vary depending on the media and type of transfer, the
main components of the aseptic techniques...show more content...
Controls are especially important when it comes to identifying unknown microbes as it is done within this report. Positive controls serve as a
comparison or model of how the performed test should react if the microbe tested is positive. This enables the experimenter to compare results and
determine if their culture is positive or negative for the reactions. In contrast, the negative controls serve to model what the test should look like if
nothing is done or if the organism tested is negative. For every laboratory exercise performed in this course, there are positive and negative control
demonstrations available for students to reference. These are very useful in aiding students when they are determining the result of their unknown
culture

Microbiology Labs
MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________
EXPERIMENT 1
TITLE: Observing Bacteria and Blood
OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation ofbacteria and
blood.
PROCEDURES: Using the microscope, an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood:
After reviewing the section of the manual as instructed I cleaned the ocular lenses and prepared the slides. I made required adjustments to the
microscope and ocular distances for view during experiment. I practiced using six prepared slides that were in the kit to ensure I was viewing...show
more content...
I examined all three slides under the microscope. After observing the slides I flushed the specimens down the drain and disinfected the area with bleach
solution. After 42–72 hours I assessed the growth patterns of the tube and noted that the tube that contained the swab became yellow stained and the
tube that contained the agar and tablet dissolved a little and some of the content drifted to the surface of the tube and created a film.
QUESTIONS:
1. There was no change from the fresh yogurt and the cultured yogurt. After 24 hours of sitting in a dark environment it became fermented and liquidy.
Mouth Swab – looks like coccobacillus; plaque: looks like single baccilus; Thick multi–layered – positive gram stain; colorless moving particles that
resemble droplets clustered together
Yeast: looks like tetrad; moving particles, no stain; colorless moving particles
Plaque: thick multi–layered clusters – gram positive stain; solid stable particles, non moving Were you able to identify specific bacterial morphologies?
Yes I was able to identify some examples as I have previously stated.

What is the difference between direct and indirect staining? Did the smears appear different in each type of staining? Why or why not?
Direct Staining involves crystal violet and staining bacterial cells causing a negative reaction; Indirect staining involves staining but results are positive
with pink solutions allowing bacteria to be viewed more accurately. These reactions are

Unknown Bacteria: Microbiology Lab Report
UNKNOW BACTERIA LAB REPORT
UNKNOWN 36
Introduction
The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help
students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because
identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped
others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.
Materials and Methods
A mixed culture of two unknown bacteria was provided by the instructor. The methods used for...show more content...
The colonies were smooth, translucent, and had a white brownish color. The Gram stain resulted in Gram positive cocci. After the Gram stain was
completed, the bacteria were streaked on a Mannitol–Salt Agar plate and a Catalase test was performed. After these test were completed a Phenol Red
Dextrose Fermentation tube was inoculated, and a SIM Tube inoculated.
The Unknown Bacteria 36/Bacteria # 2 on a TSA plate was examined by the naked eye and under a dissecting microscope. Bacteria # 2 was
approximately 3 – 4 mm in diameter. They were circular in form with an entire margin and a flat elevation. The colonies were rough (granular),
translucent, and white brownish color with black granules. The Gram stain resulted in a Gram negative rod. After the Gram stain was completed, the
bacteria were streaked on an Eosin –Methylene Blue Agar plate and an Enterotube II was inoculated.
See Table 1 and Flow Chart 1 for results of Bacteria # 1 and Table 2 and Flow Chart 2 for results of Bacteria # 2.
Table 1: Biochemical Test Results (Bacteria # 1) TEST| PURPOSE| REAGENTS| OBSERVATIONS| RESULTS| Gram Stain| To determine the Gram
reaction of Bacteria #1| Crystal violet, Iodine, Alcohol, Safranin | Purple cocci| Gram positive cocci | MSA Agar plate| To determine Gram positive
Staphylococcus species| None| Yellow halo around streak| Staphylococcus aureus| Catalase Test| To verify bacteria identification| Hydrogen peroxide|

No bubbling|

Scope Of Microbiology

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