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Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.23, 2014
80
Comparative Efficiency of Soil and Foliar Applied Zinc in
Improving Yield and Yield Components of Wheat (Triticum
aestivum L.) Variety Kiran-95
Hafeez Noor Baloch*1, Muhammad Nawaz Kandhro1, Sana Ullah Baloch2, Shahbaz Khan Baloch2, Sun
yingying2, Salih A. I. Sabiel2, Shabeer Ahmed Badini1, and Rameez Ahmed Baloch1
1. Department of Agronomy, Sindh Agriculture University, Tandojam, Sindh, Pakistan
2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430071, Hubei, China
(Corresponding author:hafeeznoorbaloch @gmail.com)
Abstract
A field study was undertaken to evaluate the comparative efficacy of zinc application through soil and foliar
spray on growth and yield of wheat variety Kiran-95 at Soil Chemistry Section, Agriculture Research Institute,
Tandojam during Rabi 2013-14. The experiment was laid out in three replicated randomized complete block
design. The treatments comprised Control (No Zinc), Soil applied Zinc 5.0 kg ha-1 at tillering stage, Soil applied
Zinc 10.0 kg ha-1 at tillering stage, Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage,
Foliar applied Zinc 0.2% at tillering stage, Foliar applied Zinc 0.4% at tillering and Foliar applied Zinc 0.2% at
tillering stage + 0.2% at booting stage. The statistical analysis of data suggested that soil and foliar applied zinc
affected significantly (P<0.05) growth and yield traits of wheat variety Kiran-95. The results illustrated that Soil
applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage produced maximum plant height (99.0 cm),
tillers (410.7 m-2), spike length (13.1 cm), spikelets per spike (23.0), grains per spike (45.1), seed index (43.4 g),
biological yield (9354.4 kg ha-1) and grain yield (5123.4 kg ha-1), closely followed by Foliar applied Zinc 0.2%
at tillering stage + 0.2% at booting stage with 97.6 cm plant height, 408.8 tillers m-2, 13.0 cm spike length, 22.8
spikelets per spike, 45.0 grains per spike, 43.2 g seed index, 9273.4 kg ha-1 biological yield and 5080.7 kg ha-1
grain yield. The performance of wheat variety Kiran-95 ranked 3rd, 4th, 5th and 6th almost in all the growth and
yield parameters particularly grain yield (kg ha-1) when fertilized with Soil applied Zinc 10.0 kg ha-1 at tillering
stage, Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied
Zinc 0.2% at tillering stage. However, minimum growth and yield traits were registered in Control (No Zinc).
Furthermore, the results concluded that although numerically maximum growth and yield values were recorded
in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically the differences between
Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage and Foliar applied Zinc 0.2% at tillering
stage + 0.2% at booting stage were non-significant. Hence, Foliar applied Zinc 0.2% at tillering stage + 0.2% at
booting stage were found the most economical treatment for obtaining optimum yield of wheat variety Kiran-95.
Keywords: Wheat, Comparative Efficiency, Soil, Foliar, Zinc, Improving Yield and Yield Components
1. Introduction 1
Wheat (Triticum aestivum L.) is one of the most important crops in the world. It is the leading food grain of
Pakistan and being staple diet of the people. Wheat occupies a central position in formulation of agricultural
policies by contributing 10.1 percent to the value added in agriculture and 2.2 percent to GDP. Wheat was
cultivated on an area of 8693 thousand hectares during 2012-13 with production of 24231 thousand tonnes (GOP,
2013). Wheat yield in Pakistan is 2787 kg ha-1 as against 4762 kg ha-1 in China, world average 3086 kg ha-1,
3018 kg ha-1 and in the USA. The average yield per unit area of wheat in Pakistan is still far below the potential
yields due to a variety of factors.
Micronutrients play a pivotal role in the yield improvement of cereals (Rehm and Sims, 2006). Zn is classified
as a micronutrient. Almost 50 percent of the world soils used for cereal production is Zn deficient (Torun et al.,
2001); as a result, approximately 2 billion people suffer from Zn deficiency all over the world (Asad and Rafique,
2002). A deficiency of Zn is characterized by the development of broad bands of striped tissue on each side of
the midrib of the leaf. A Zn deficient plant also appears to be stunted, commonly known as "little leaf" (Thalooth
et al., 2006). Almost 50 percent of the world soils used for cereal production is Zn deficient (Torun et al., 2001);
as a result, approximately 2 billion people suffer from Zn deficiency all over the world (Asad and Rafique, 2002).
Zinc is essential for the transformation of carbohydrates and regulating consumption of sugars. It is required in a
variety of enzymes and plays an essential role in DNA transcription (Don Eckert, 2010). The grain yield can be
improved by addition of Zn fertilization (Maqsood et al., 2009).
Zinc deficiency is a worldwide nutritional constraint for crop production, as Zn removed by crops is usually not
fully replenished by fertilization in agricultural soils. Its deficiency is particularly widespread in cereals that are
grown on calcareous soil (Graham et al., 1992). Deficiency of zinc in wheat has been reported from various parts
of the world, Pakistan soils are not exception to this. Almost 50% of the world soils used for cereal production
are Zn deficient (Gibbson, 2006). This percentage is even higher in areas with calcareous soils as the proportion
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.23, 2014
81
of sorbed Zn that could be desorbed back into solution decreased substantially as pH increased to more than 5.5
(Singh et al., 2008), which reduces not only grain yield, but also nutritional quality of grains. As a result,
approximately two billion people suffer from Zn deficiency all over the world. Zinc deficiency is the third most
serious crop nutrition problem in the Pakistan, ranking after N and P deficiency (Rashid & Rafique, 1996). Zinc
deficiency is common in both warm and cold climate. Despite of being graded as less sensitive (Clark, 1990),
wheat is severely affected by Zn deficiency in Pakistan.
Foliar fertilization of crops can complement and guarantee the availability of nutrients to crops for obtaining
higher yields (Arif et al., 2006). The foliar spray of zinc at tillering and/or booting and milking growth stage (s)
increased the grain yield of wheat (Mohammad et al., 2009). Sohu (2008) disclosed that soil application of Zn
had significant effect on all the growth and grain yield contributing parameters of wheat. The highest Zn level of
15.0 kg ha-1 produced significantly greater values for plant height (93.26 cm), number of tillers (13.86) plant-1,
spike length (12.33 cm), number of grains (62.2) spike-1, seed index (39.27 g) and grain yield (4966.67 kg ha-1).
Application of zinc increased wheat dry matter, grain yield, and straw yield significantly over control (Asad and
Rafique, 2000). Similarly, in another study Zeidan et al. (2010) concluded that wheat grain yield, straw yield,
1000-grain weight and number of grains spike¬1 and Zn concentration in flag leaves and grains as well as,
protein content in grain were significantly increased by the application of zinc.
1.1 Materials and Methods 2
The field study was conducted at Soil Chemistry Section, Agriculture Research Institute, Tandojam to evaluate
the comparative efficacy of soil and foliar applied zinc in improving yield and yield components of wheat variety
Kiran-95 during Rabi, 2012-13. The experiment was laid out in three replicated randomized complete block
design (RCBD), having net plot size 5 m x 4 m (20 m2).
Land preparation
The land was prepared by two dry plowings followed by precision land leveling. After soaking doze, when soil
reached proper moisture level, two plowings with cultivator plow were done to achieve the fine seedbed.
Sowing time and method
The sowing was done with the help of single row hand drill on 10th November, 2013, maintaining distance of
22.5 cm between rows.
Irrigation and fertilizer application
The first irrigation was applied at the crown root initiation stage i.e. 21 DAS. Subsequently five irrigations were
applied as and when needed until the crop reached physiological maturity. Inorganic fertilizers were used to
provide proper nutrition to plants. Nitrogen was applied at 120 kg N ha-1 in the form of Urea and DAP. Urea was
applied in three splits, first at the time of sowing, second at the 1st irrigation and the remaining at the 2nd
irrigation. Full dose of phosphorus in the form of DAP @ 56 kg ha-1 was applied at the time of sowing.
Crop harvesting
At maturity five plants from each treatment of all replications were selected at random for harvest. These plants
were harvested by cutting at ground level with sharp sickle. The spikes were separated from straw, placed in
separate paper bags, oven-dried for 24 hours at 78oC. Threshing was done by hands.
Statistical Analysis
The data were subjected to statistical analysis using MSTAT-C (Russel and Eisensmith, 1983). The Duncan’s
Multiple Range Test (DMRT) was applied to compare treatment means superiority, where necessary.
1.1.1 Results 3
The field study was conducted at Soil Chemistry Section, Agriculture Research Institute, Tandojam to evaluate
the comparative efficacy of soil and foliar applied zinc in improving yield and yield components of wheat variety
Kiran-95 during Rabi, 2012-13. The experiment was laid out in three replicated randomized complete block
design (RCBD). The net plot size was kept as 5 m x 4 m (20 m2
). The treatments included: Control (No Zinc),
Soil applied Zinc 5.0 kg ha-1
at tillering stage, Soil applied Zinc 10.0 kg ha-1
at tillering stage, Soil applied Zinc
5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage, Foliar applied Zinc 0.2% at tillering stage, Foliar applied
Zinc 0.4% at tillering and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. The observations
were recorded on parameters of economic importance such as Plant height (cm), Tillers (m-2
), Spike length (cm),
Spikelets per spike, Grains per spike, Seed index (1000-grain weight, g), Biological yield (kg ha-1
) and Grain
yield (kg ha-1
). The results on above parameters are shown in Tables 1-8 and their analyses of variance as
Appendices I-VIII. The results are interpreted as under:
Plant height (cm)
The data in relation to plant height (cm) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are
shown in Table-1 and their analysis of variance as Appendix-I. The analysis of variance showed that the
differences for plant height (cm) between various treatments were statistically significant (P<0.05). The results
illustrated that maximum plant height (99.0 cm) was recorded in plots fertilized with Zinc 5.0 kg ha-1
at tillering
+ 5.0 kg ha-1
at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage
and Soil applied Zinc 10.0 kg ha-1
at tillering stage which resulted in 97.6 and 94.2 cm plant height, respectively.
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.23, 2014
82
The plant height reduced to 93.7, 92.0 and 90.9 cm when crop was fertilized with Foliar applied Zinc 0.4% at
tillering, Soil applied Zinc 5.0 kg ha-1
at tillering stage and Foliar applied Zinc 0.2% at tillering stage,
respectively. However, minimum plant height (88.5 cm) was noted in Control (No Zinc). Furthermore, results
indicated that although numerically maximum plant height was recorded in Soil applied Zinc 5.0 kg ha-1
at
tillering + 5.0 kg ha-1
at booting stage but statistically it differed non-significantly (P>0.05%) with Foliar applied
Zinc 0.2% at tillering stage + 0.2% at booting stage.
Table 1.
Effect of soil and foliar applied zinc on plant height (cm) of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 89.9 84.9 90.9 88.5 d
Soil applied Zinc 5.0 kg ha-1
at tillering stage 92.3 91.4 92.3 92.0 bc
Soil applied Zinc 10.0 kg ha-1
at tillering stage 94.3 94.4 93.9 94.2 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage 99.2 99.0 98.9 99.0 a
Foliar applied Zinc 0.2% at tillering stage 89.9 89.9 92.9 90.9 cd
Foliar applied Zinc 0.4% at tillering 93.9 93.4 93.9 93.7 b
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 97.8 98.0 96.9 97.6 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
Tillers (m-2
)
The results regarding tillers (m-2
) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are
presented in Table-2. The analysis of variance indicated that the differences for tillers (m-2
) between various
treatments were statistically significant (P<0.05). The results suggested that maximum tillers (410.7 m-2
) were
noted in plots fertilized with Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage, closely followed by Foliar
applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1
at tillering stage with
408.8 and 395.7 tillers m-2
, respectively. The number of tillers m-2
reduced to 392.3, 387.3 and 385.3 when crop was
supplied with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1
at tillering stage and Foliar applied
Zinc 0.2% at tillering stage, respectively. However, minimum tillers (381.5 m-2
) were recorded in Control (No
Zinc). Moreover, data showed that although numerically maximum tillers (m-2
) were observed in Soil applied Zinc
5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage but they demonstrated non-significant (P>0.05%) differences
statistically when compared with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage.
Table 2. Effect of soil and foliar applied zinc on tillers (m-2
) of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 376.5 386.5 381.5 381.5 d
Soil applied Zinc 5.0 kg ha-1
at tillering stage 389.6 384.6 387.6 387.3 cd
Soil applied Zinc 10.0 kg ha-1
at tillering stage 398.0 393.0 396.0 395.7 bc
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at
booting stage
421.0 411.0 400.0 410.7 a
Foliar applied Zinc 0.2% at tillering stage 387.6 382.6 385.6 385.3 cd
Foliar applied Zinc 0.4% at tillering 394.6 389.6 392.6 392.3 cd
Foliar applied Zinc 0.2% at tillering stage + 0.2% at
booting stage
412.5 405.5 408.5 408.8 ab
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
Spike length (cm)
The data pertaining to spike length (cm) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are
shown in Table-3. The analysis of variance showed that the differences for spike length (cm) between various
S.E ± 1.1198
DMR 0.05 2.4398
DMR 0.01 3.4204
CV% 1.46
S.E ± 3.9009
DMR 0.05 13.636
DMR 0.01 17.433
CV% 1.21
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.23, 2014
83
treatments were statistically significant (P<0.05). The results exhibited that maximum spike length (13.1 cm) was
recorded in plots fertilized with Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage, closely followed by
Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1
at tillering stage
which resulted in 13.0 and 12.4 cm spike length, respectively. The spike length reduced to 11.4, 11.3 and 9.9 cm
when crop was fertilized with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1
at tillering stage
and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum spike length (9.6 cm) was noted in
Control (No Zinc). Furthermore, results indicated that although numerically maximum spike length was recorded in
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage but statistically it differed non-significantly
(P>0.05%) with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage.
Table 3.
Effect of soil and foliar applied zinc on spike length (cm) of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 9.6 9.7 9.6 9.6 d
Soil applied Zinc 5.0 kg ha-1
at tillering stage 11.3 11.4 11.3 11.3 c
Soil applied Zinc 10.0 kg ha-1
at tillering stage 12.4 12.2 12.6 12.4 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at
booting stage
13.2 13.1 13.1 13.1 a
Foliar applied Zinc 0.2% at tillering stage 10.5 9.8 9.5 9.9 d
Foliar applied Zinc 0.4% at tillering 11.5 11.3 11.4 11.4 c
Foliar applied Zinc 0.2% at tillering stage + 0.2% at
booting stage
13.1 13.0 12.9 13.0 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
Spikelets per spike
The results in relation to spikelets per spike of wheat variety Kiran-95 as affected by soil and foliar applied zinc
are given in Table-4. The analysis of variance suggested that the differences for spikelets per spike between
various treatments were statistically significant (P<0.05). The results illustrated that maximum spikelets per
spike (23.0) were found in plots fertilized with Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage, closely
followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1
at
tillering stage with 22.8 and 22.4 spikelets per spike, respectively. The number of spikelets per spike decreased
to 22.1, 21.0 and 19.5 under Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1
at tillering stage
and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum spikelets per spike (18.9) were
registered under Control (No Zinc). In addition, results indicated that although numerically maximum spikelets
per spike were recorded in Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage but statistically
non-significant (P>0.05%) differences were observed in comparison with Foliar applied Zinc 0.2% at tillering
stage + 0.2% at booting stage.
Table 4. Effect of soil and foliar applied zinc on spikelets per spike of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 18.9 19.0 18.8 18.9 e
Soil applied Zinc 5.0 kg ha-1
at tillering stage 21.0 21.0 21.0 21.0 c
Soil applied Zinc 10.0 kg ha-1
at tillering stage 22.4 22.3 22.5 22.4 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage 23.1 23.0 23.1 23.0 b
Foliar applied Zinc 0.2% at tillering stage 19.7 19.3 19.5 19.5 d
Foliar applied Zinc 0.4% at tillering 22.1 22.0 22.2 22.1 b
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 22.7 23.0 22.8 22.8 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
S.E ± 0.1735
DMR 0.05 0.6064
DMR 0.01 0.7753
CV% 1.84
S.E ± 0.0947
DMR 0.05 0.3311
DMR 0.01 0.4233
CV% 0.54
Journal of Biology, Agriculture and Healthcare www.iiste.org
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Vol.4, No.23, 2014
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Grains per spike
The results regarding grains per spike of wheat variety Kiran-95 as affected by soil and foliar applied zinc are
presented in Table-5 and their analysis of variance as Appendix-V. The analysis of variance indicated that the
differences for grains per spike between various treatments were statistically significant (P<0.05). The results
suggested that maximum grains per spike (45.1) were noted in plots fertilized with Zinc 5.0 kg ha-1
at tillering + 5.0
kg ha-1
at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil
applied Zinc 10.0 kg ha-1
at tillering stage with 45.0 and 44.0 grains per spike, respectively. The number of grains
per spike reduced to 43.0, 42.6 and 37.7 when crop was supplied with Foliar applied Zinc 0.4% at tillering, Soil
applied Zinc 5.0 kg ha-1
at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However,
minimum grains per spike (36.5) were recorded in Control (No Zinc). Moreover, data showed that although
numerically maximum grains per spike were observed in Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at
booting stage but they demonstrated non-significant (P>0.05%) differences statistically when compared with Foliar
applied Zinc 0.2% at tillering stage + 0.2% at booting stage.
Table 5. Effect of soil and foliar applied zinc on grains per spike of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 36.4 36.7 36.3 36.5 e
Soil applied Zinc 5.0 kg ha-1
at tillering stage 42.6 42.7 42.5 42.6 c
Soil applied Zinc 10.0 kg ha-1
at tillering stage 43.6 44.2 44.3 44.0 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage 45.3 45.0 45.2 45.1 a
Foliar applied Zinc 0.2% at tillering stage 38.0 37.3 37.6 37.7 d
Foliar applied Zinc 0.4% at tillering 42.7 43.4 43.0 43.0 c
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 45.0 45.2 44.9 45.0 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
Seed index (1000-grain weight, g)
The data pertaining to Seed index (1000-grain weight, g) of wheat variety Kiran-95 as affected by soil and foliar
applied zinc are shown in Table-6 and their analysis of variance as Appendix-VI. The analysis of variance showed
that the differences for seed index (g) between various treatments were statistically significant (P<0.05). The results
demonstrated that maximum seed index (43.4 g) was noticed in plots fertilized with Zinc 5.0 kg ha-1
at tillering +
5.0 kg ha-1
at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and
Soil applied Zinc 10.0 kg ha-1
at tillering stage which resulted in 43.2 and 39.8 g seed index, respectively. The seed
index decreased to 37.7, 37.0 and 35.6 g when crop was fertilized with Foliar applied Zinc 0.4% at tillering, Soil
applied Zinc 5.0 kg ha-1
at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However,
minimum seed index (33.7 g) was noted in Control (No Zinc). Furthermore, results indicated that although
numerically maximum seed index was recorded in Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting
stage but statistically it differed non-significantly (P>0.05%) with Foliar applied Zinc 0.2% at tillering stage +
0.2% at booting stage.
Table 6. Effect of soil and foliar applied zinc on seed index (1000-grain weight, g) of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 33.6 33.9 33.5 33.7 d
Soil applied Zinc 5.0 kg ha-1
at tillering stage 36.4 37.0 37.4 37.0 bcd
Soil applied Zinc 10.0 kg ha-1
at tillering stage 39.3 39.9 40.3 39.8 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage 43.7 42.7 43.7 43.4 a
Foliar applied Zinc 0.2% at tillering stage 38.7 33.3 34.8 35.6 cd
Foliar applied Zinc 0.4% at tillering 37.2 37.8 38.2 37.7 bc
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 43.6 43.2 42.9 43.2 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
Biological yield (kg ha-1
)
The data in relation to biological yield (kg ha-1
) of wheat variety Kiran-95 as affected by soil and foliar applied
S.E ± 0.2136
DMR 0.05 0.7465
DMR 0.01 0.9545
CV% 0.62
S.E ± 0.9581
DMR 0.05 3.3491
DMR 0.01 4.2820
CV% 3.04
Journal of Biology, Agriculture and Healthcare www.iiste.org
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Vol.4, No.23, 2014
85
zinc are shown in Table-7 and their analysis of variance as Appendix-VII. The analysis of variance showed that
the differences for biological yield (kg ha-1
) between various treatments were statistically significant (P<0.05).
The results illustrated that maximum biological yield (9354.4 kg ha-1
) was recorded in plots fertilized with Zinc
5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage
+ 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1
at tillering stage which resulted in 9273.4 and 8440.4 kg
ha-1
biological yield, respectively. The biological yield reduced to 7650.7, 7415.9 and 7050.2 kg ha-1
when crop
was fertilized with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1
at tillering stage and Foliar
applied Zinc 0.2% at tillering stage, respectively. However, minimum biological yield (6940.6 kg ha-1
) was noted
in Control (No Zinc). In addition, results indicated that although numerically maximum biological yield was
recorded in Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage but statistically it differed non-
significantly (P>0.05%) with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage.
Table 7. Effect of soil and foliar applied zinc on biological yield (kg ha-1
) of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 6931.0 6989.7 6901.2 6940.6 c
Soil applied Zinc 5.0 kg ha-1
at tillering stage 7423.5 7529.2 7294.9 7415.9 c
Soil applied Zinc 10.0 kg ha-1
at tillering stage 8320.1 8173.9 8827.3 8440.4 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage 9405.0 9272.0 9386.2 9354.4 a
Foliar applied Zinc 0.2% at tillering stage 7666.2 6532.8 6951.5 7050.2 c
Foliar applied Zinc 0.4% at tillering 7587.9 7739.7 7624.6 7650.7 c
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 9262.7 9314.0 9243.5 9273.4 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
Grain yield (kg ha-1
)
The data regarding grain yield (kg ha-1
) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are
presented in Table-8 whereas, the analysis of variance as Appendix-VIII. The analysis of variance indicated that
the differences for grain yield (kg ha-1
) between various treatments were statistically significant (P<0.05). The
results showed that maximum grain yield (5123.4 kg ha-1
) was observed in plots fertilized with Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2%
booting stage and Soil applied Zinc 10.0 kg ha-1
at tillering stage with 5080.7 and 4638.0 kg ha-1
grain yield,
respectively. The grain yield decreased to 4222.4, 4098.8 and 3906.3 kg ha-1
under Foliar applied Zinc 0.4% at
tillering, Soil applied Zinc 5.0 kg ha-1
at tillering stage and Foliar applied Zinc 0.2% at tillering stage,
respectively. However, minimum grain yield (3848.7 kg ha-1
) was recorded in Control (No Zinc). Furthermore,
results suggested that although numerically maximum grain yield was recorded under Soil applied Zinc 5.0 kg
ha-1
at tillering + 5.0 kg ha-1
at booting stage but statistically non-significant (P>0.05%) differences were found
when compared with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage.
Table 8. Effect of soil and foliar applied zinc on grain yield (kg ha-1
) of wheat variety Kiran-95
Treatments R-I R-II R-III Mean
Control (No Zinc) 3843.6 3874.5 3827.9 3848.7 c
Soil applied Zinc 5.0 kg ha-1
at tillering stage 4102.8 4158.4 4035.1 4098.8 c
Soil applied Zinc 10.0 kg ha-1
at tillering stage 4574.7 4497.8 4841.6 4638.0 b
Soil applied Zinc 5.0 kg ha-1
at tillering + 5.0 kg ha-1
at booting stage 5150.0 5080.0 5140.1 5123.4 a
Foliar applied Zinc 0.2% at tillering stage 4230.5 3634.0 3854.4 3906.3 c
Foliar applied Zinc 0.4% at tillering 4189.3 4269.2 4208.6 4222.4 c
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 5075.1 5102.1 5065.0 5080.7 a
Means not sharing the same letter in a column differ significantly at 0.05 probability level.
1.1.2 Discussion 3
Nutrient deficiency and imbalanced fertilizers use are one of the important factors for low yield of wheat in
Pakistan. Zinc (Zn) is an essential micronutrient for plant growth and its deficiency is common in cultivated soils
S.E ± 219.71
DMR 0.05 767.99
DMR 0.01 981.90
CV% 3.36
S.E ± 115.64
DMR 0.05 404.20
DMR 0.01 516.79
CV% 3.21
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.23, 2014
86
of Pakistan (Zeidan et al., 2010). Zinc plays a very important role for the transformation of carbohydrates and
regulating consumption of sugars. It is required in a variety of enzymes and plays an essential role in DNA
transcription (Don Eckert, 2010). A typical symptom of zinc deficiency is the stunted growth of leaves,
commonly known as "little leaf" (Thalooth et al., 2006).The grain yield can be improved by addition of Zn
fertilization (Maqsood et al., 2009). Foliar fertilization of crops can complement and guarantee the availability of
nutrients to crops for obtaining higher yields (Arif et al., 2006). The findings of the study suggested that soil and
foliar applied zinc affected significantly (P<0.05) growth and yield traits of wheat variety Kiran-95. Soil applied
Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage produced maximum plant height (99.0 cm), tillers
(410.7 m-2), spike length (13.1 cm), spikelets per spike (23.0), grains per spike (45.1), seed index (43.4 g),
biological yield (9354.4 kg ha-1) and grain yield (5123.4 kg ha-1), closely followed by Foliar applied Zinc 0.2%
at tillering stage + 0.2% at booting stage almost in all the growth and yield attributes, particularly grain yield
(5080.7 kg ha-1). However, minimum growth and yield traits particularly grain yield (5080.7 kg ha-1).
Furthermore, the results concluded that although numerically maximum growth and yield values were recorded
in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically the differences between
Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage and Foliar applied Zinc 0.2% at tillering
stage + 0.2% at booting stage were non-significant. These results showed that foliar application of zinc proved
more beneficial and economical as compared to soil applied zinc. Low dose of zinc is required when applied
through spray. Application of zinc twice at tillering and booting stage demonstrated more effectiveness as
compared to application once at higher or lower dose either through foliar application or soil. These results
confirmed that zinc is required at two critical stages i.e. tillering and booting stage. The results are in agreement
with those of Arif et al. (2006) who suggested that Foliar fertilization of crops can complement and guarantee the
availability of nutrients to crops for obtaining higher yields The results are in line with the findings of
Mohammad et al. (2009) who concluded that foliar spray of zinc at tillering and/or booting and milking growth
stage (s) increased the grain yield of wheat. Similar results were also reported by Sohu (2008) who found that
soil application of Zn had significant positive effect on all the growth and yield contributing parameters of wheat.
The results are strongly supported by Asad and Rafique (2000) who indicated that Application of zinc increased
wheat dry matter, grain yield, and straw yield significantly over control. Similarly, in another study Zeidan et al.
(2010) concluded that wheat grain yield, straw yield, 1000-grain weight and number of grains spike¬1 and Zn
concentration in flag leaves and grains as well as, protein content in grain were significantly increased by the
application of zinc. Dell et al. (2001) disclosed that Foliar treatments with ZnSO4 and chelated Zn forms resulted
in shoot Zn concentrations in 7-week-old plants being about two-fold greater than those in plants supplied with
Zn in the root environment or via foliar spray of ZnO. Adding surfactant to foliar sprays containing chelated
forms of Zn did not cause negative growth effects, but surfactant added to ZnO or ZnSO4 foliar sprays decreased
shoot growth. Adding urea to the ZnO foliar spray had no effect on shoot growth. Foliarly-applied 65 Zn was
translocated to leaves above and below the treated leaf as well as to the root tips. These results are in accordance
with Hussain and Yasin (2004) who reported 12% increase in wheat yield by the application of 5 kg Zn ha-1,
over control. Increasing Zn dose upto 16 kg Zn ha-1 increased spike length, number of spikelets per spike,
number of tillers per m2 and 1000 grain weight, significantly over control, while highest straw yield was
obtained with the application of 8 kg Zn ha-1. In conclusion, Zn supply along with recommended NPK fertilizers
led to satisfactory crop production. The complimentary use of micronutrients is advantageous as it helps increase
NPK uptake, maintain micronutrients levels in soil and increases crop productivity. It is possible to obtain higher
crop yield with complimentary use of micronutrients along with recommended macronutrient fertilizers.
Conclusion
The results concluded that all the treatments of soil and foliar applied zinc affected significantly (P<0.05) growth
and yield parameters of wheat variety Kiran-95. Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at
booting stage (T4) produced maximum traits, particularly grain yield (5123.4 kg ha-1), closely followed by
Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage (T7) with 5080.7 kg ha-1 grain yield.
However, T7 was found the most economical for obtaining optimum yield of wheat variety Kiran-95 due to non-
significant (P>0.05) statistical differences with T4.
References
Abbas, G., M.Q. Khan, M. Jamil, M. Tahir and F. Hussain. 2009. Nutrient uptake, growth and yield of wheat
(Triticum aestivum) as affected by zinc application rates. Int. J. Agri. Biol., 11: 389-396.
Ali, S., A. Shah, M. Arif, G. Miraj, I. Ali, M. Sajjad, Farhatullah, M.Y. Khan and N.M. Khan. 2009.
Enhancement of wheat grain yield and yield components through foliar application of Zinc and Boron.
Sarhad J. Agri., 25(1):15-19.
Arif, M., M.A. Khan, H. Akbar and S. Ali. 2006. Prospects of wheat as a dual purpose crop and its impact on
weeds. Pak. J. Weed Sci. Res., 12 (1-2): 13-17.
Asad, A. and R. Rafique. 2000. Effect of Zinc, Copper, Iron, Manganese and Boron on the yield and yield
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.23, 2014
87
components of wheat crop in Tehsil Peshawar. Pak. J. Biol. Sci., 3(10):1815-1820.
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Bagci, S.A., H. Ekiz, A. Yilmaz and I. Cakmak. 2007. Effects of Zinc Deficiency and Drought on Grain Yield of
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Don Eckert, 2010. Efficient Fertilizer Use - Nitrogen, Pp. 1-19.
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Manganese Applications and Uptake and Concentration of Zinc, Iron, and Manganese in Wheat Grains.
Commun. Soil Sci. Plant Anal., 40 (7/8): 1322-1332.
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soil humidity, plant physiology yield components. Meidity, 10:60-63.
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effect on uptake of nutrients. Int. J. Trop. Agri., 21(1-4): 161-166.
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Thalooth, A.T., M.M. Tawfik and H. M. Mohamed. 2006. Comparative study on the effect of foliar application of
zinc, potassium and magnesium on growth, yield and some chemical constituents of mungbean plants
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Comparative efficacy of soil and foliar applied zinc in improving wheat yield

  • 1. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 80 Comparative Efficiency of Soil and Foliar Applied Zinc in Improving Yield and Yield Components of Wheat (Triticum aestivum L.) Variety Kiran-95 Hafeez Noor Baloch*1, Muhammad Nawaz Kandhro1, Sana Ullah Baloch2, Shahbaz Khan Baloch2, Sun yingying2, Salih A. I. Sabiel2, Shabeer Ahmed Badini1, and Rameez Ahmed Baloch1 1. Department of Agronomy, Sindh Agriculture University, Tandojam, Sindh, Pakistan 2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430071, Hubei, China (Corresponding author:hafeeznoorbaloch @gmail.com) Abstract A field study was undertaken to evaluate the comparative efficacy of zinc application through soil and foliar spray on growth and yield of wheat variety Kiran-95 at Soil Chemistry Section, Agriculture Research Institute, Tandojam during Rabi 2013-14. The experiment was laid out in three replicated randomized complete block design. The treatments comprised Control (No Zinc), Soil applied Zinc 5.0 kg ha-1 at tillering stage, Soil applied Zinc 10.0 kg ha-1 at tillering stage, Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, Foliar applied Zinc 0.2% at tillering stage, Foliar applied Zinc 0.4% at tillering and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. The statistical analysis of data suggested that soil and foliar applied zinc affected significantly (P<0.05) growth and yield traits of wheat variety Kiran-95. The results illustrated that Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage produced maximum plant height (99.0 cm), tillers (410.7 m-2), spike length (13.1 cm), spikelets per spike (23.0), grains per spike (45.1), seed index (43.4 g), biological yield (9354.4 kg ha-1) and grain yield (5123.4 kg ha-1), closely followed by Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage with 97.6 cm plant height, 408.8 tillers m-2, 13.0 cm spike length, 22.8 spikelets per spike, 45.0 grains per spike, 43.2 g seed index, 9273.4 kg ha-1 biological yield and 5080.7 kg ha-1 grain yield. The performance of wheat variety Kiran-95 ranked 3rd, 4th, 5th and 6th almost in all the growth and yield parameters particularly grain yield (kg ha-1) when fertilized with Soil applied Zinc 10.0 kg ha-1 at tillering stage, Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage. However, minimum growth and yield traits were registered in Control (No Zinc). Furthermore, the results concluded that although numerically maximum growth and yield values were recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically the differences between Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage were non-significant. Hence, Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage were found the most economical treatment for obtaining optimum yield of wheat variety Kiran-95. Keywords: Wheat, Comparative Efficiency, Soil, Foliar, Zinc, Improving Yield and Yield Components 1. Introduction 1 Wheat (Triticum aestivum L.) is one of the most important crops in the world. It is the leading food grain of Pakistan and being staple diet of the people. Wheat occupies a central position in formulation of agricultural policies by contributing 10.1 percent to the value added in agriculture and 2.2 percent to GDP. Wheat was cultivated on an area of 8693 thousand hectares during 2012-13 with production of 24231 thousand tonnes (GOP, 2013). Wheat yield in Pakistan is 2787 kg ha-1 as against 4762 kg ha-1 in China, world average 3086 kg ha-1, 3018 kg ha-1 and in the USA. The average yield per unit area of wheat in Pakistan is still far below the potential yields due to a variety of factors. Micronutrients play a pivotal role in the yield improvement of cereals (Rehm and Sims, 2006). Zn is classified as a micronutrient. Almost 50 percent of the world soils used for cereal production is Zn deficient (Torun et al., 2001); as a result, approximately 2 billion people suffer from Zn deficiency all over the world (Asad and Rafique, 2002). A deficiency of Zn is characterized by the development of broad bands of striped tissue on each side of the midrib of the leaf. A Zn deficient plant also appears to be stunted, commonly known as "little leaf" (Thalooth et al., 2006). Almost 50 percent of the world soils used for cereal production is Zn deficient (Torun et al., 2001); as a result, approximately 2 billion people suffer from Zn deficiency all over the world (Asad and Rafique, 2002). Zinc is essential for the transformation of carbohydrates and regulating consumption of sugars. It is required in a variety of enzymes and plays an essential role in DNA transcription (Don Eckert, 2010). The grain yield can be improved by addition of Zn fertilization (Maqsood et al., 2009). Zinc deficiency is a worldwide nutritional constraint for crop production, as Zn removed by crops is usually not fully replenished by fertilization in agricultural soils. Its deficiency is particularly widespread in cereals that are grown on calcareous soil (Graham et al., 1992). Deficiency of zinc in wheat has been reported from various parts of the world, Pakistan soils are not exception to this. Almost 50% of the world soils used for cereal production are Zn deficient (Gibbson, 2006). This percentage is even higher in areas with calcareous soils as the proportion
  • 2. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 81 of sorbed Zn that could be desorbed back into solution decreased substantially as pH increased to more than 5.5 (Singh et al., 2008), which reduces not only grain yield, but also nutritional quality of grains. As a result, approximately two billion people suffer from Zn deficiency all over the world. Zinc deficiency is the third most serious crop nutrition problem in the Pakistan, ranking after N and P deficiency (Rashid & Rafique, 1996). Zinc deficiency is common in both warm and cold climate. Despite of being graded as less sensitive (Clark, 1990), wheat is severely affected by Zn deficiency in Pakistan. Foliar fertilization of crops can complement and guarantee the availability of nutrients to crops for obtaining higher yields (Arif et al., 2006). The foliar spray of zinc at tillering and/or booting and milking growth stage (s) increased the grain yield of wheat (Mohammad et al., 2009). Sohu (2008) disclosed that soil application of Zn had significant effect on all the growth and grain yield contributing parameters of wheat. The highest Zn level of 15.0 kg ha-1 produced significantly greater values for plant height (93.26 cm), number of tillers (13.86) plant-1, spike length (12.33 cm), number of grains (62.2) spike-1, seed index (39.27 g) and grain yield (4966.67 kg ha-1). Application of zinc increased wheat dry matter, grain yield, and straw yield significantly over control (Asad and Rafique, 2000). Similarly, in another study Zeidan et al. (2010) concluded that wheat grain yield, straw yield, 1000-grain weight and number of grains spike¬1 and Zn concentration in flag leaves and grains as well as, protein content in grain were significantly increased by the application of zinc. 1.1 Materials and Methods 2 The field study was conducted at Soil Chemistry Section, Agriculture Research Institute, Tandojam to evaluate the comparative efficacy of soil and foliar applied zinc in improving yield and yield components of wheat variety Kiran-95 during Rabi, 2012-13. The experiment was laid out in three replicated randomized complete block design (RCBD), having net plot size 5 m x 4 m (20 m2). Land preparation The land was prepared by two dry plowings followed by precision land leveling. After soaking doze, when soil reached proper moisture level, two plowings with cultivator plow were done to achieve the fine seedbed. Sowing time and method The sowing was done with the help of single row hand drill on 10th November, 2013, maintaining distance of 22.5 cm between rows. Irrigation and fertilizer application The first irrigation was applied at the crown root initiation stage i.e. 21 DAS. Subsequently five irrigations were applied as and when needed until the crop reached physiological maturity. Inorganic fertilizers were used to provide proper nutrition to plants. Nitrogen was applied at 120 kg N ha-1 in the form of Urea and DAP. Urea was applied in three splits, first at the time of sowing, second at the 1st irrigation and the remaining at the 2nd irrigation. Full dose of phosphorus in the form of DAP @ 56 kg ha-1 was applied at the time of sowing. Crop harvesting At maturity five plants from each treatment of all replications were selected at random for harvest. These plants were harvested by cutting at ground level with sharp sickle. The spikes were separated from straw, placed in separate paper bags, oven-dried for 24 hours at 78oC. Threshing was done by hands. Statistical Analysis The data were subjected to statistical analysis using MSTAT-C (Russel and Eisensmith, 1983). The Duncan’s Multiple Range Test (DMRT) was applied to compare treatment means superiority, where necessary. 1.1.1 Results 3 The field study was conducted at Soil Chemistry Section, Agriculture Research Institute, Tandojam to evaluate the comparative efficacy of soil and foliar applied zinc in improving yield and yield components of wheat variety Kiran-95 during Rabi, 2012-13. The experiment was laid out in three replicated randomized complete block design (RCBD). The net plot size was kept as 5 m x 4 m (20 m2 ). The treatments included: Control (No Zinc), Soil applied Zinc 5.0 kg ha-1 at tillering stage, Soil applied Zinc 10.0 kg ha-1 at tillering stage, Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, Foliar applied Zinc 0.2% at tillering stage, Foliar applied Zinc 0.4% at tillering and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. The observations were recorded on parameters of economic importance such as Plant height (cm), Tillers (m-2 ), Spike length (cm), Spikelets per spike, Grains per spike, Seed index (1000-grain weight, g), Biological yield (kg ha-1 ) and Grain yield (kg ha-1 ). The results on above parameters are shown in Tables 1-8 and their analyses of variance as Appendices I-VIII. The results are interpreted as under: Plant height (cm) The data in relation to plant height (cm) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are shown in Table-1 and their analysis of variance as Appendix-I. The analysis of variance showed that the differences for plant height (cm) between various treatments were statistically significant (P<0.05). The results illustrated that maximum plant height (99.0 cm) was recorded in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage which resulted in 97.6 and 94.2 cm plant height, respectively.
  • 3. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 82 The plant height reduced to 93.7, 92.0 and 90.9 cm when crop was fertilized with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum plant height (88.5 cm) was noted in Control (No Zinc). Furthermore, results indicated that although numerically maximum plant height was recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically it differed non-significantly (P>0.05%) with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 1. Effect of soil and foliar applied zinc on plant height (cm) of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 89.9 84.9 90.9 88.5 d Soil applied Zinc 5.0 kg ha-1 at tillering stage 92.3 91.4 92.3 92.0 bc Soil applied Zinc 10.0 kg ha-1 at tillering stage 94.3 94.4 93.9 94.2 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 99.2 99.0 98.9 99.0 a Foliar applied Zinc 0.2% at tillering stage 89.9 89.9 92.9 90.9 cd Foliar applied Zinc 0.4% at tillering 93.9 93.4 93.9 93.7 b Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 97.8 98.0 96.9 97.6 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. Tillers (m-2 ) The results regarding tillers (m-2 ) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are presented in Table-2. The analysis of variance indicated that the differences for tillers (m-2 ) between various treatments were statistically significant (P<0.05). The results suggested that maximum tillers (410.7 m-2 ) were noted in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage with 408.8 and 395.7 tillers m-2 , respectively. The number of tillers m-2 reduced to 392.3, 387.3 and 385.3 when crop was supplied with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum tillers (381.5 m-2 ) were recorded in Control (No Zinc). Moreover, data showed that although numerically maximum tillers (m-2 ) were observed in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but they demonstrated non-significant (P>0.05%) differences statistically when compared with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 2. Effect of soil and foliar applied zinc on tillers (m-2 ) of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 376.5 386.5 381.5 381.5 d Soil applied Zinc 5.0 kg ha-1 at tillering stage 389.6 384.6 387.6 387.3 cd Soil applied Zinc 10.0 kg ha-1 at tillering stage 398.0 393.0 396.0 395.7 bc Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 421.0 411.0 400.0 410.7 a Foliar applied Zinc 0.2% at tillering stage 387.6 382.6 385.6 385.3 cd Foliar applied Zinc 0.4% at tillering 394.6 389.6 392.6 392.3 cd Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 412.5 405.5 408.5 408.8 ab Means not sharing the same letter in a column differ significantly at 0.05 probability level. Spike length (cm) The data pertaining to spike length (cm) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are shown in Table-3. The analysis of variance showed that the differences for spike length (cm) between various S.E ± 1.1198 DMR 0.05 2.4398 DMR 0.01 3.4204 CV% 1.46 S.E ± 3.9009 DMR 0.05 13.636 DMR 0.01 17.433 CV% 1.21
  • 4. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 83 treatments were statistically significant (P<0.05). The results exhibited that maximum spike length (13.1 cm) was recorded in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage which resulted in 13.0 and 12.4 cm spike length, respectively. The spike length reduced to 11.4, 11.3 and 9.9 cm when crop was fertilized with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum spike length (9.6 cm) was noted in Control (No Zinc). Furthermore, results indicated that although numerically maximum spike length was recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically it differed non-significantly (P>0.05%) with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 3. Effect of soil and foliar applied zinc on spike length (cm) of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 9.6 9.7 9.6 9.6 d Soil applied Zinc 5.0 kg ha-1 at tillering stage 11.3 11.4 11.3 11.3 c Soil applied Zinc 10.0 kg ha-1 at tillering stage 12.4 12.2 12.6 12.4 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 13.2 13.1 13.1 13.1 a Foliar applied Zinc 0.2% at tillering stage 10.5 9.8 9.5 9.9 d Foliar applied Zinc 0.4% at tillering 11.5 11.3 11.4 11.4 c Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 13.1 13.0 12.9 13.0 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. Spikelets per spike The results in relation to spikelets per spike of wheat variety Kiran-95 as affected by soil and foliar applied zinc are given in Table-4. The analysis of variance suggested that the differences for spikelets per spike between various treatments were statistically significant (P<0.05). The results illustrated that maximum spikelets per spike (23.0) were found in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage with 22.8 and 22.4 spikelets per spike, respectively. The number of spikelets per spike decreased to 22.1, 21.0 and 19.5 under Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum spikelets per spike (18.9) were registered under Control (No Zinc). In addition, results indicated that although numerically maximum spikelets per spike were recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically non-significant (P>0.05%) differences were observed in comparison with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 4. Effect of soil and foliar applied zinc on spikelets per spike of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 18.9 19.0 18.8 18.9 e Soil applied Zinc 5.0 kg ha-1 at tillering stage 21.0 21.0 21.0 21.0 c Soil applied Zinc 10.0 kg ha-1 at tillering stage 22.4 22.3 22.5 22.4 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 23.1 23.0 23.1 23.0 b Foliar applied Zinc 0.2% at tillering stage 19.7 19.3 19.5 19.5 d Foliar applied Zinc 0.4% at tillering 22.1 22.0 22.2 22.1 b Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 22.7 23.0 22.8 22.8 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. S.E ± 0.1735 DMR 0.05 0.6064 DMR 0.01 0.7753 CV% 1.84 S.E ± 0.0947 DMR 0.05 0.3311 DMR 0.01 0.4233 CV% 0.54
  • 5. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 84 Grains per spike The results regarding grains per spike of wheat variety Kiran-95 as affected by soil and foliar applied zinc are presented in Table-5 and their analysis of variance as Appendix-V. The analysis of variance indicated that the differences for grains per spike between various treatments were statistically significant (P<0.05). The results suggested that maximum grains per spike (45.1) were noted in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage with 45.0 and 44.0 grains per spike, respectively. The number of grains per spike reduced to 43.0, 42.6 and 37.7 when crop was supplied with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum grains per spike (36.5) were recorded in Control (No Zinc). Moreover, data showed that although numerically maximum grains per spike were observed in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but they demonstrated non-significant (P>0.05%) differences statistically when compared with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 5. Effect of soil and foliar applied zinc on grains per spike of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 36.4 36.7 36.3 36.5 e Soil applied Zinc 5.0 kg ha-1 at tillering stage 42.6 42.7 42.5 42.6 c Soil applied Zinc 10.0 kg ha-1 at tillering stage 43.6 44.2 44.3 44.0 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 45.3 45.0 45.2 45.1 a Foliar applied Zinc 0.2% at tillering stage 38.0 37.3 37.6 37.7 d Foliar applied Zinc 0.4% at tillering 42.7 43.4 43.0 43.0 c Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 45.0 45.2 44.9 45.0 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. Seed index (1000-grain weight, g) The data pertaining to Seed index (1000-grain weight, g) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are shown in Table-6 and their analysis of variance as Appendix-VI. The analysis of variance showed that the differences for seed index (g) between various treatments were statistically significant (P<0.05). The results demonstrated that maximum seed index (43.4 g) was noticed in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage which resulted in 43.2 and 39.8 g seed index, respectively. The seed index decreased to 37.7, 37.0 and 35.6 g when crop was fertilized with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum seed index (33.7 g) was noted in Control (No Zinc). Furthermore, results indicated that although numerically maximum seed index was recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically it differed non-significantly (P>0.05%) with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 6. Effect of soil and foliar applied zinc on seed index (1000-grain weight, g) of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 33.6 33.9 33.5 33.7 d Soil applied Zinc 5.0 kg ha-1 at tillering stage 36.4 37.0 37.4 37.0 bcd Soil applied Zinc 10.0 kg ha-1 at tillering stage 39.3 39.9 40.3 39.8 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 43.7 42.7 43.7 43.4 a Foliar applied Zinc 0.2% at tillering stage 38.7 33.3 34.8 35.6 cd Foliar applied Zinc 0.4% at tillering 37.2 37.8 38.2 37.7 bc Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 43.6 43.2 42.9 43.2 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. Biological yield (kg ha-1 ) The data in relation to biological yield (kg ha-1 ) of wheat variety Kiran-95 as affected by soil and foliar applied S.E ± 0.2136 DMR 0.05 0.7465 DMR 0.01 0.9545 CV% 0.62 S.E ± 0.9581 DMR 0.05 3.3491 DMR 0.01 4.2820 CV% 3.04
  • 6. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 85 zinc are shown in Table-7 and their analysis of variance as Appendix-VII. The analysis of variance showed that the differences for biological yield (kg ha-1 ) between various treatments were statistically significant (P<0.05). The results illustrated that maximum biological yield (9354.4 kg ha-1 ) was recorded in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage which resulted in 9273.4 and 8440.4 kg ha-1 biological yield, respectively. The biological yield reduced to 7650.7, 7415.9 and 7050.2 kg ha-1 when crop was fertilized with Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum biological yield (6940.6 kg ha-1 ) was noted in Control (No Zinc). In addition, results indicated that although numerically maximum biological yield was recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically it differed non- significantly (P>0.05%) with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 7. Effect of soil and foliar applied zinc on biological yield (kg ha-1 ) of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 6931.0 6989.7 6901.2 6940.6 c Soil applied Zinc 5.0 kg ha-1 at tillering stage 7423.5 7529.2 7294.9 7415.9 c Soil applied Zinc 10.0 kg ha-1 at tillering stage 8320.1 8173.9 8827.3 8440.4 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 9405.0 9272.0 9386.2 9354.4 a Foliar applied Zinc 0.2% at tillering stage 7666.2 6532.8 6951.5 7050.2 c Foliar applied Zinc 0.4% at tillering 7587.9 7739.7 7624.6 7650.7 c Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 9262.7 9314.0 9243.5 9273.4 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. Grain yield (kg ha-1 ) The data regarding grain yield (kg ha-1 ) of wheat variety Kiran-95 as affected by soil and foliar applied zinc are presented in Table-8 whereas, the analysis of variance as Appendix-VIII. The analysis of variance indicated that the differences for grain yield (kg ha-1 ) between various treatments were statistically significant (P<0.05). The results showed that maximum grain yield (5123.4 kg ha-1 ) was observed in plots fertilized with Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, closely followed by Foliar applied Zinc 0.2% tillering stage + 0.2% booting stage and Soil applied Zinc 10.0 kg ha-1 at tillering stage with 5080.7 and 4638.0 kg ha-1 grain yield, respectively. The grain yield decreased to 4222.4, 4098.8 and 3906.3 kg ha-1 under Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage, respectively. However, minimum grain yield (3848.7 kg ha-1 ) was recorded in Control (No Zinc). Furthermore, results suggested that although numerically maximum grain yield was recorded under Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically non-significant (P>0.05%) differences were found when compared with Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. Table 8. Effect of soil and foliar applied zinc on grain yield (kg ha-1 ) of wheat variety Kiran-95 Treatments R-I R-II R-III Mean Control (No Zinc) 3843.6 3874.5 3827.9 3848.7 c Soil applied Zinc 5.0 kg ha-1 at tillering stage 4102.8 4158.4 4035.1 4098.8 c Soil applied Zinc 10.0 kg ha-1 at tillering stage 4574.7 4497.8 4841.6 4638.0 b Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage 5150.0 5080.0 5140.1 5123.4 a Foliar applied Zinc 0.2% at tillering stage 4230.5 3634.0 3854.4 3906.3 c Foliar applied Zinc 0.4% at tillering 4189.3 4269.2 4208.6 4222.4 c Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage 5075.1 5102.1 5065.0 5080.7 a Means not sharing the same letter in a column differ significantly at 0.05 probability level. 1.1.2 Discussion 3 Nutrient deficiency and imbalanced fertilizers use are one of the important factors for low yield of wheat in Pakistan. Zinc (Zn) is an essential micronutrient for plant growth and its deficiency is common in cultivated soils S.E ± 219.71 DMR 0.05 767.99 DMR 0.01 981.90 CV% 3.36 S.E ± 115.64 DMR 0.05 404.20 DMR 0.01 516.79 CV% 3.21
  • 7. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 86 of Pakistan (Zeidan et al., 2010). Zinc plays a very important role for the transformation of carbohydrates and regulating consumption of sugars. It is required in a variety of enzymes and plays an essential role in DNA transcription (Don Eckert, 2010). A typical symptom of zinc deficiency is the stunted growth of leaves, commonly known as "little leaf" (Thalooth et al., 2006).The grain yield can be improved by addition of Zn fertilization (Maqsood et al., 2009). Foliar fertilization of crops can complement and guarantee the availability of nutrients to crops for obtaining higher yields (Arif et al., 2006). The findings of the study suggested that soil and foliar applied zinc affected significantly (P<0.05) growth and yield traits of wheat variety Kiran-95. Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage produced maximum plant height (99.0 cm), tillers (410.7 m-2), spike length (13.1 cm), spikelets per spike (23.0), grains per spike (45.1), seed index (43.4 g), biological yield (9354.4 kg ha-1) and grain yield (5123.4 kg ha-1), closely followed by Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage almost in all the growth and yield attributes, particularly grain yield (5080.7 kg ha-1). However, minimum growth and yield traits particularly grain yield (5080.7 kg ha-1). Furthermore, the results concluded that although numerically maximum growth and yield values were recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically the differences between Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage were non-significant. These results showed that foliar application of zinc proved more beneficial and economical as compared to soil applied zinc. Low dose of zinc is required when applied through spray. Application of zinc twice at tillering and booting stage demonstrated more effectiveness as compared to application once at higher or lower dose either through foliar application or soil. These results confirmed that zinc is required at two critical stages i.e. tillering and booting stage. The results are in agreement with those of Arif et al. (2006) who suggested that Foliar fertilization of crops can complement and guarantee the availability of nutrients to crops for obtaining higher yields The results are in line with the findings of Mohammad et al. (2009) who concluded that foliar spray of zinc at tillering and/or booting and milking growth stage (s) increased the grain yield of wheat. Similar results were also reported by Sohu (2008) who found that soil application of Zn had significant positive effect on all the growth and yield contributing parameters of wheat. The results are strongly supported by Asad and Rafique (2000) who indicated that Application of zinc increased wheat dry matter, grain yield, and straw yield significantly over control. Similarly, in another study Zeidan et al. (2010) concluded that wheat grain yield, straw yield, 1000-grain weight and number of grains spike¬1 and Zn concentration in flag leaves and grains as well as, protein content in grain were significantly increased by the application of zinc. Dell et al. (2001) disclosed that Foliar treatments with ZnSO4 and chelated Zn forms resulted in shoot Zn concentrations in 7-week-old plants being about two-fold greater than those in plants supplied with Zn in the root environment or via foliar spray of ZnO. Adding surfactant to foliar sprays containing chelated forms of Zn did not cause negative growth effects, but surfactant added to ZnO or ZnSO4 foliar sprays decreased shoot growth. Adding urea to the ZnO foliar spray had no effect on shoot growth. Foliarly-applied 65 Zn was translocated to leaves above and below the treated leaf as well as to the root tips. These results are in accordance with Hussain and Yasin (2004) who reported 12% increase in wheat yield by the application of 5 kg Zn ha-1, over control. Increasing Zn dose upto 16 kg Zn ha-1 increased spike length, number of spikelets per spike, number of tillers per m2 and 1000 grain weight, significantly over control, while highest straw yield was obtained with the application of 8 kg Zn ha-1. In conclusion, Zn supply along with recommended NPK fertilizers led to satisfactory crop production. The complimentary use of micronutrients is advantageous as it helps increase NPK uptake, maintain micronutrients levels in soil and increases crop productivity. It is possible to obtain higher crop yield with complimentary use of micronutrients along with recommended macronutrient fertilizers. Conclusion The results concluded that all the treatments of soil and foliar applied zinc affected significantly (P<0.05) growth and yield parameters of wheat variety Kiran-95. Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage (T4) produced maximum traits, particularly grain yield (5123.4 kg ha-1), closely followed by Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage (T7) with 5080.7 kg ha-1 grain yield. However, T7 was found the most economical for obtaining optimum yield of wheat variety Kiran-95 due to non- significant (P>0.05) statistical differences with T4. References Abbas, G., M.Q. Khan, M. Jamil, M. Tahir and F. Hussain. 2009. 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  • 8. Journal of Biology, Agriculture and Healthcare www.iiste.org ISSN 2224-3208 (Paper) ISSN 2225-093X (Online) Vol.4, No.23, 2014 87 components of wheat crop in Tehsil Peshawar. Pak. J. Biol. Sci., 3(10):1815-1820. Asad, A., and R. Rafique, 2002. Identification of micronutrient deficiency of wheat in the Peshawar valley, Pakistan. Commun. Soil Sci. Plant Anal., 33 (3/4):349-364. Bagci, S.A., H. Ekiz, A. Yilmaz and I. Cakmak. 2007. Effects of Zinc Deficiency and Drought on Grain Yield of Field-grown Wheat Cultivars in Central Anatolia. J. Agron. Crop Sci., 193(3):198-206. Don Eckert, 2010. Efficient Fertilizer Use - Nitrogen, Pp. 1-19. Dell, B., L. Huang and R.W. Bell. 2002. Boron in plant reproduction. p. 103-108. In: Boron in plant and Animal Nutrition, H.E. Goldbach B. Rerkasem, M. Wimmer, P.H. Brown, M. Thellier and R.W. Bell eds.). Kluwer Academic/Plenum Publishers, New Yark. GOP. 2013. Area, production and yield per hectare of wheat. Agricultural Statistics of Pakistan, 2012-13. Ministry of Food, Agriculture and Livestock, Government of Pakistan, Economic Advisory Wing, Finance Division, Islamabad. Hussain, F. and M. Yasin. 2004. Soil fertility monitoring and management in rice-wheat system, Pp: 1–33. Annual Report, LRRP, NARC, Islamabad, Pakistan Laghari, M.I.K. 2011. Grain yield of wheat as influenced by different zinc and boron application methods. M.Sc. Thesis (Agronomy) submitted to Sindh Agriculture University Tandojam. Maqsood, M.A., Rahmatullah, S. Kanwal, T. Aziz and M. Ashraf. 2009. Evaluation of Zn distribution among grain and straw of twelve indigenous wheat, Triticum aestivum L. genotypes. Pak. J. Bot., 41(1): 225-231. Mohammad, R., A. Pahlavan-Rad and M. Pessarakli. 2009. Response of Wheat Plants to Zinc, Iron, and Manganese Applications and Uptake and Concentration of Zinc, Iron, and Manganese in Wheat Grains. Commun. Soil Sci. Plant Anal., 40 (7/8): 1322-1332. Naceu, M.B., M. Naily and M.Selmi. 2006. Effect of water deficiency during different growth stages of wheat on soil humidity, plant physiology yield components. Meidity, 10:60-63. Nurcan, K., S. Eker and I. Cakmak. 2004. “Capsule”: Cadmium toxicity in shoots was alleviated by zinc treatments. Environ. Pollut., 131 (3) : 453-459. Rehm, G. and A. Sims. 2006. Micronutrients and production of hard red spring wheat. Minnesota Crop News, Univ., Minnesota. Russel, D.F. and S.P. Eisensmith. 1983. MSTAT-C. Crop Soil Sci. Department Michigan State Univ. U.S.A. Seilsepour, M. 2006. Study of Zinc effects on quantitative and qualitative traits of winter wheat in saline soil condition. Biaban (Desert Journal), 11 (2): 17-23. Singh, S., and S.S. Choudhary. 2003. Effect of phosphorus, zinc and soils on yield of wheat and their interactive effect on uptake of nutrients. Int. J. Trop. Agri., 21(1-4): 161-166. Sohu, I.A. 2008. Growth and productivity response of wheat to different proportions zinc. Indus J. Plant Sci., 5 (1/2): 45-48. Thalooth, A.T., M.M. Tawfik and H. M. Mohamed. 2006. Comparative study on the effect of foliar application of zinc, potassium and magnesium on growth, yield and some chemical constituents of mungbean plants grown under water stress conditions. World J. Agril. Sci., 2 (1): 37-46. Torun, A., I. Gültekin, M. Kalayci, A. Yilmaz and S. Eker. 2001. Effects of zinc fertilization on grain yield and shoot concentrations of zinc, boron, and phosphorus of 25 wheat cultivars grown on a zinc-deficient and boron-toxic soil. J. Plant Nutri., 24 (11): 1817-1829. Unar, G.A. 2004. To study the impact of macro and micro nutrients on the yield and yield parameters of wheat. M.Sc. Thesis (Agronomy) submitted to Sindh Agriculture University Tandojam. Zeidan, M.S., M. F. Mohamed and H.A. Hamouda. 2010. Effect of foliar fertilization of Fe, Mn and Zn on wheat yield and quality in low sandy soils fertility. World J. Agril. Sci., 6(6):696-699.
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