Steps to microbial metobolic processes . Steps to measure microbial growth

1. PRESENTED BY :- PRESENTED TO :-
 NAME :- ASHUTOSH JENA
 SEMESTER :- 1ST
 SESSION :- 2020-22
 DEPARTMENT OF EDUCATION
 DR. SURENDER SINGH
 ASSOCIATE PROFESSOR
 DEPARTMENT OF MICROBIOLOGY
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2. Metabolism
1. Catabolism
2. Anabolism
Reduction
1. Gains electrons
2. E.g - hydrocarbons, methanes, fats, carbohydrates,
alcohols
Oxidation
1. Loses electrons
2. E.g – ketones, aldehyde, carboxylic acids
Oxido-Redox Reactions
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3. Micro organisms grow = size and number
Culture media
Methods
1. Total Count (Direct Method)
2. Viable Count (Indirect Method)
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4. We count the colonies here.
One colony has nearly 60-70 microbes.
It is of two types
1. Counting chamber method
2. Electron counter method
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 Colony Counter  Haemocytometer

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7. It is of types
1. Serial dilution method
2. Membrane filter method
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11.  The ATP concentration of cell extract and cell number
relationship is linear.
 Bioluminiscence based detection –
 The light emitted by luciferin is detected by
luminometer.
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12.  Dehydrogenase enzyme produced in biological
processes could be used as an indicator for the
biological wastewater treatment.
 Present study introduces a simple and modified
method for evaluation of biological wastewater
treatment process measuring dehydrogenase activity.
 In the present study, the effective parameters such as
incubation time and types of solvent were investigated
and the best procedure is developed for measuring the
dehydrogenase activity in biological wastewater
treatment process.
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14. https://www.slideshare.net/drmalathi13/microbia
l-metabolism-47468219
https://www.slideshare.net/NehaKaushal8/micro
bial-metabolism-76935314
https://www.slideshare.net/AbdulRehman944/me
thods-of-measuring-microbial-growth
https://www.slideshare.net/SalmanAli83/bacteria
l-growth-58131516
https://www.slideshare.net/Pure-man/chapter-3-
microbial-growth
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