We at 3Brain are committed to advancing scientific research and boosting drug discovery. Like our technology, our product lines are always evolving to accommodate high-resolution recording of in vitro cultures. Discover our HD-MEA technology and soon-to-be-released devices and see how they are furthering research in brain diseases, drug discovery, retinal organoids, etc..
For more information, visit our website at https://www.3brain.com
2. Imaging at different spatio-temporal scales
Macro- Meso- Micro-
100 billion neurons 2D/3D cultures, organoids,
tens of thousands of cells
Neurons and sub-elements
3. Competing technology
1.5mm
Main limitations
• temporal resolution
• field of view / spatial resolution
• indirect measurement: use of dyes
(averaging, bleaching, interference with
network parameters)
0.1mm
Main limitations:
• very low spatial resolution (electrode pitch
~100 μm)
• large under-sampling of network activity
(~20,000 neurons per 100 electrodes)
Optical imaging Extracellular electrophysiology
4. 3Brain’s BioCAM – BioChip Advantages
>0.5
0
Values[mV]
Spontaneous retinal waves of a P10 mouse under bicuculline effect
Standard MEAHigh-density MEA
6. HD-MEA Probes
• 4,096 electrodes
• new 3D models coming
soon
BioCAM Line
• single- and multi- well
models
BrainWave 4
• data recording, analysis
and compression tools
Services
• data analysis, training,
protocols and
experimental design
Products and services
Solutions
Help
Care
9. Pharmaceutical applications
First 6-well prototype
coming soon
• Multi-well configuration
• Advanced data compression strategies
• Roadmap to increase # on wells: 12, 96, 384 and
more
From single-well… …to multi-well
11. 24 Days In Vitro (DIV)
100nM
Oligomer Aβ-42
26 Hours of monitoring
H. Amin et al., Sci Report 2017
Dissociated hippocampal
neuronal network
In vitro model of induced Alzheimer’s disease
12. Human neuronal cultures on HD-MEA
validated for more than 3 months with
spontaneous and electrically evoked
recordings
Amin et al., Front. Neurosci. 2016
Several adhesion factors (PDLO,
PEI) promote different
maturation stages
Human-derived stem cell neuronal networks