Cartilages.histology

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This presentation was made by various histology slides of cartilages (taken from web pages) as a pretest for the exams. Not for any other commercial purposes.

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Hyaline Cartilage is pretty straight forward. Look for the isogenous groups in the homogenous matrix. You shouldn't have much trouble here.

Differentiating elastic cartilage from fibrocartilage (and loose connective tissue and nerves and Wharton's Jelly, and etc.) is more difficult. On the light microscope you can "focus through" the cartilage to find the elastic fibers. But even so, it can still be difficult so be sure to spend some time on it.When I took histology, there was one thing I did learn: if all else fails, guess fibrocartilage. Try following slides.

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Cartilage: Cartilage is a specialized type of dense collective tissue designed to give support , bear weight and withstand tension , torsion , and bending. General Features : • Cartilage supports regions of body that requires flexibility. • Non nervous structure • Avascular • Very poor regeneration power • Usually surrounded by pericondrium (dense irregular connective tissue surrounding cartilage) except fibro cartilage. Classification:  Hyaline cartilage  Elastic cartilage  Fibro cartilage Fibro Cartilage : White colored, tough cartilage containing dense connective tissue and collagen fibers often known as intervertebral discs is called fibro cartilage. Structure :  Fibro cartilage consists of chondrocytes dispersed among bundles of type 1 collagen fibers.  Chondrocytes are present in lacunae (cavity).  The arrangement of cells is different from all other type of cartilages.  Chondrocytes are arranged in parallel rows of 2, 4 or 6 cells.  These rows of cells are called isogenous cell groups. Chemical Compounds Present : Proteoglycans rich in sulphated glucosaminoglycans especially  Chondroiton sulphate  Dermatan sulphate Stain :  Due to the abundance of collagen type 1 fibers , the matrix of fibrocartilage stains intensely acidophilic/eosinophilic. (since collagen is basic in nature)  Stained by EOSIN which is pink in color.  Chondrocytes are stained in purple usually by HEMATOXYLIN and looks purple in color due to acidic nature of large centeral nucleus present. Occurrence in body :  Intervertebral disc  Disc of pubic symphysis  Menisci of knee joint  Sternoclavicular joint  Temporomandibular joint  Ligamentum tere  Labrum glenoidale  Labrum acetabulare  Fibrocartilage is also found at places where tendons and ligaments attach to bones. Disorders:  Degeneration of fibrocartilage is seen in degenerative disc disease.  A fibrocartilaginous embolism (FCE) is an unusual cause of spinal cord and cerebral ischemia (insufficient bloodsupply). Symptoms may include sudden, severe pain in the neck and/or back; progressive weakening reduced sensation and paralysis. It may be caused by the blocking of an artery interrupting vascular supply.  A herniated disk is a disk that ruptures. This allows the jelly-like center of the disk to leak, irritating the nearby nerves. This can cause sciatica or back pain. References:  http://www.nlm.nih.gov/medlineplus/herniateddisk.html  http://www.ncbi.nlm.nih.gov/pubmed/3289246  histology by laiq hussaain

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detail notes on connective tissue.. Connective tissue (CT) is one of the four basic types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. It develops from the mesoderm. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. In the central nervous system, the three outer membranes (the meninges) that envelop the brain and spinal cord are composed of connective tissue. All connective tissue consists of three main components: fibers (elastic and collagenous fibers), ground substance and cells. Not all authorities include blood or lymph as connective tissue because they lack the fiber component. All are immersed in the body water.

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