Histology slides snapshots (first year mbbs)

Usama Nasir
Usama NasirWriter at Elance
HISTOLOGY SLIDES
FIRST YEAR MBBS
Rawalpindi Medical College
SIMPLE SQUAMOUS EPITHELIUM
Identification points:
• Flattened cells
• Surface view: rounded central nucleus
• Cross section: elongated nucleus
Histology slides  snapshots (first year mbbs)
SIMPLE CUBOIDAL EPITHELIUM
Identification Points:
• Cuboidal cells (height almost the same as width)
• Central rounded nuclei
• Surface view: polygonal
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
SIMPLE COLUMNAR EPITHELIUM
Identification points:
• Single layer of elongated cells
• Height of cell > width of cell
• Elongated basal nuclei
Histology slides  snapshots (first year mbbs)
SIMPLE COLUMNAR EPITHELIUM WITH
GOBLET CELLS
Identification Points:
• Elongated column shaped cells (height > width)
• Elongated basal nuclei
• Goblet cells have central and oval nuclei
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
STRATIFIED CUBOIDAL EPITHELIUM
Identification points:
• Multilayered
• Basal cells: high cuboidal or low columnar
• Surface layer: cuboidal
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
STRATIFIED SQUAMOUS NON
KERATINIZED EPITHELIUM
Identification Points:
• Surface squamous flattened cells
• No keratin
• Middle layer: polygonal cells
• Basal layer: high cuboidal/low columnar cells
Histology slides  snapshots (first year mbbs)
PSEUDO STRATIFIED COLUMNAR
EPITHELIUM
Identification Points:
• Single layer of cells
• Some cells are columnar; others are cuboidal
• Nuclei lying at different levels; give the false appearance of ‘stratified’
epithelium
EPIDIDYMUS
Histology slides  snapshots (first year mbbs)
TRANSITIONAL EPITHELIUM
Identification Points:
• Basal layer of cuboidal cells
• Next, four layers of polygonal cells
• Next, two layers of pear shaped cells
• Upper most layer of umbrella shaped cells
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
THIN SKIN
Identification Points:
• Stratified squamous keratinized epithelium
• Dense irregular CT beneath
• Hair follicles and roots present
• Sebaceous glands
• Sweat glands
Histology slides  snapshots (first year mbbs)
THICK SKIN
Identification Points:
• Stratified squamous keratinized epithelium
• No hair roots and follicles
• Pacinian corpuscles
Histology slides  snapshots (first year mbbs)
DENSE REGULAR C.T
Identification Points:
• Parallel collagen fibres in the form of bundles
• Compressed elongated fibroblasts between the collagen fibres
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
MUCOUS ACINI
Identification Points:
• Pyramidal cells
• Apical portion lightly stained
• Basal portion darkly stained
• Flattened nuclei towards the base
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
FIBRO CARTILAGE
Identification Points:
• No perichondrium
• Chondrocytes within lacunae, arranged in rows
• Collagen fibres arranged parallel
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
ELASTIC CARTILAGE
Identification Points:
• Perichondrium is present
• Elastic fibres in matrix
• Basophilic ground substance around lacunae
• Chondrocytes in lacunae in the form of isogenous groups
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
HYALINE CARTILAGE
Identification Points:
• Dense Irregular CT (perichondrium)
• Chondrocytes within lacunae
• Chondrocytes in the form of isogenous groups
• Naked eye examination: Bluish white translucent appearance
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Costal Hyaline cartilage
ARTICULAR CARTILAGE
Identification Points:
• Superficial tangential layer
• Intermediate transitional layer
• Radial zone
• Calcified zone
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
COMPACT BONE
Identification Points:
• Haversion Canal
• Lamellae
• Osteocytes in lacunae
• Volkmann’s canal
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
SPONGY BONE
Identification Points:
• Trabeculae
• Red marrow
• Spikes
• Periosteum: outer fibrous, inner cellular
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
CARDIAC MUSCLE
Identification Points:
• Branched
• Striated
• Intercalated discs
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
SMOOTH MUSCLE
Identification points:
• Non striated appearance
• Single central nucleus
• Spindle shaped cells
Histology slides  snapshots (first year mbbs)
SKELETAL MUSCLES
Identification Points:
• Characteristic striated appearance
• Peripheral nuclei
• Nucleus ovoid in shape
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
PERIPHERAL NERVE
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
CEREBRUM
Identification points:
• Pyramidal and non pyramidal cells
• Arranged in the form of layers (mention the names) – properly visible only
by ‘Nissl staining method’
Histology slides  snapshots (first year mbbs)
CEREBELLUM
Identification Points:
From outwards to inwards:
• Molecular layer with fan shaped dendrites, stellate cells and basket cells
(but cell population is very low)
• Flask shaped Perkinge cells
• Granular layer (deeply basophilic appearance of closely packed nuclei)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
SPINAL CORD
Identification Points:
• Central canal
• Butterfly shaped grey matter
• Dorsal median sulcus and ventral median fissure (depending on the
portion under examination)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
ELASTIC ARTERY
Identification Points:
• No distinct outer and inner elastic membranes
• Tunica adventitia: merges with CT, contains vasa vasorum
• Tunica media: elastic fibres are present in a large amount
• Tunica intima: Simple squamous endothelium, subendothelial CT
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
MUSCULAR ARTERY
Identification Points:
• Tunica intima: darkly stained
• Tunica media: smooth muscle fibres arranged in circular patterns
• Tunica adventitia: collagen (lightly stained); elastic (darkly stained)
Histology slides  snapshots (first year mbbs)
LYMPH NODE
Identification Points:
• Primary follicles/nodules without germinal centre
• Secondary follicles/nodules with germinal centre
• Irregular anastomosing ‘medullary cords’
• Subcapsular sinuses
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
THYMUS
Identification Points:
• Medulla is lightly stained
• Hassall’s corpuscles in medulla
• Epithelioreticular cells; stellate shaped
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
SPLEEN
Identification Points:
• Red pulp: splenic cord and venous sinuses
• White pulp: contains lymphatic nodules and germinal centre
• Splenic sinuses
• No differentiation into cortex and medulla
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
TONSIL
Identification Points:
• Stratified squamous non keratinized epithelium
• Crypt
• Trabeculae
• Lymphatic nodules
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
TRACHEA
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
LUNG
Identification Points:
• Alveoli: Type I alveolar cells (simple squamous epithelium); Type II alveolar
cells (cuboidal cells)
• Respiratory bronchioles: lined by simple cuboidal epithelium
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
ADIPOSE TISSUE
(WHITE)
Identification Points:
• Unilocular
• Nuclei towards periphery
• Spherical
Histology slides  snapshots (first year mbbs)
Histology slides  snapshots (first year mbbs)
THE END

By Nowera Zafar
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Histology slides snapshots (first year mbbs)