Histology slides snapshots (first year mbbs)

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Histology slides snapshots (first year mbbs)

  1. 1. HISTOLOGY SLIDES FIRST YEAR MBBS Rawalpindi Medical College
  2. 2. SIMPLE SQUAMOUS EPITHELIUM Identification points: • Flattened cells • Surface view: rounded central nucleus • Cross section: elongated nucleus
  3. 3. SIMPLE CUBOIDAL EPITHELIUM Identification Points: • Cuboidal cells (height almost the same as width) • Central rounded nuclei • Surface view: polygonal
  4. 4. SIMPLE COLUMNAR EPITHELIUM Identification points: • Single layer of elongated cells • Height of cell > width of cell • Elongated basal nuclei
  5. 5. SIMPLE COLUMNAR EPITHELIUM WITH GOBLET CELLS Identification Points: • Elongated column shaped cells (height > width) • Elongated basal nuclei • Goblet cells have central and oval nuclei
  6. 6. STRATIFIED CUBOIDAL EPITHELIUM Identification points: • Multilayered • Basal cells: high cuboidal or low columnar • Surface layer: cuboidal
  7. 7. STRATIFIED SQUAMOUS NON KERATINIZED EPITHELIUM Identification Points: • Surface squamous flattened cells • No keratin • Middle layer: polygonal cells • Basal layer: high cuboidal/low columnar cells
  8. 8. PSEUDO STRATIFIED COLUMNAR EPITHELIUM Identification Points: • Single layer of cells • Some cells are columnar; others are cuboidal • Nuclei lying at different levels; give the false appearance of ‘stratified’ epithelium
  9. 9. EPIDIDYMUS
  10. 10. TRANSITIONAL EPITHELIUM Identification Points: • Basal layer of cuboidal cells • Next, four layers of polygonal cells • Next, two layers of pear shaped cells • Upper most layer of umbrella shaped cells
  11. 11. THIN SKIN Identification Points: • Stratified squamous keratinized epithelium • Dense irregular CT beneath • Hair follicles and roots present • Sebaceous glands • Sweat glands
  12. 12. THICK SKIN Identification Points: • Stratified squamous keratinized epithelium • No hair roots and follicles • Pacinian corpuscles
  13. 13. DENSE REGULAR C.T Identification Points: • Parallel collagen fibres in the form of bundles • Compressed elongated fibroblasts between the collagen fibres
  14. 14. MUCOUS ACINI Identification Points: • Pyramidal cells • Apical portion lightly stained • Basal portion darkly stained • Flattened nuclei towards the base
  15. 15. FIBRO CARTILAGE Identification Points: • No perichondrium • Chondrocytes within lacunae, arranged in rows • Collagen fibres arranged parallel
  16. 16. ELASTIC CARTILAGE Identification Points: • Perichondrium is present • Elastic fibres in matrix • Basophilic ground substance around lacunae • Chondrocytes in lacunae in the form of isogenous groups
  17. 17. HYALINE CARTILAGE Identification Points: • Dense Irregular CT (perichondrium) • Chondrocytes within lacunae • Chondrocytes in the form of isogenous groups • Naked eye examination: Bluish white translucent appearance
  18. 18. Costal Hyaline cartilage
  19. 19. ARTICULAR CARTILAGE Identification Points: • Superficial tangential layer • Intermediate transitional layer • Radial zone • Calcified zone
  20. 20. COMPACT BONE Identification Points: • Haversion Canal • Lamellae • Osteocytes in lacunae • Volkmann’s canal
  21. 21. SPONGY BONE Identification Points: • Trabeculae • Red marrow • Spikes • Periosteum: outer fibrous, inner cellular
  22. 22. CARDIAC MUSCLE Identification Points: • Branched • Striated • Intercalated discs
  23. 23. SMOOTH MUSCLE Identification points: • Non striated appearance • Single central nucleus • Spindle shaped cells
  24. 24. SKELETAL MUSCLES Identification Points: • Characteristic striated appearance • Peripheral nuclei • Nucleus ovoid in shape
  25. 25. PERIPHERAL NERVE
  26. 26. CEREBRUM Identification points: • Pyramidal and non pyramidal cells • Arranged in the form of layers (mention the names) – properly visible only by ‘Nissl staining method’
  27. 27. CEREBELLUM Identification Points: From outwards to inwards: • Molecular layer with fan shaped dendrites, stellate cells and basket cells (but cell population is very low) • Flask shaped Perkinge cells • Granular layer (deeply basophilic appearance of closely packed nuclei)
  28. 28. SPINAL CORD Identification Points: • Central canal • Butterfly shaped grey matter • Dorsal median sulcus and ventral median fissure (depending on the portion under examination)
  29. 29. ELASTIC ARTERY Identification Points: • No distinct outer and inner elastic membranes • Tunica adventitia: merges with CT, contains vasa vasorum • Tunica media: elastic fibres are present in a large amount • Tunica intima: Simple squamous endothelium, subendothelial CT
  30. 30. MUSCULAR ARTERY Identification Points: • Tunica intima: darkly stained • Tunica media: smooth muscle fibres arranged in circular patterns • Tunica adventitia: collagen (lightly stained); elastic (darkly stained)
  31. 31. LYMPH NODE Identification Points: • Primary follicles/nodules without germinal centre • Secondary follicles/nodules with germinal centre • Irregular anastomosing ‘medullary cords’ • Subcapsular sinuses
  32. 32. THYMUS Identification Points: • Medulla is lightly stained • Hassall’s corpuscles in medulla • Epithelioreticular cells; stellate shaped
  33. 33. SPLEEN Identification Points: • Red pulp: splenic cord and venous sinuses • White pulp: contains lymphatic nodules and germinal centre • Splenic sinuses • No differentiation into cortex and medulla
  34. 34. TONSIL Identification Points: • Stratified squamous non keratinized epithelium • Crypt • Trabeculae • Lymphatic nodules
  35. 35. TRACHEA
  36. 36. LUNG Identification Points: • Alveoli: Type I alveolar cells (simple squamous epithelium); Type II alveolar cells (cuboidal cells) • Respiratory bronchioles: lined by simple cuboidal epithelium
  37. 37. ADIPOSE TISSUE (WHITE) Identification Points: • Unilocular • Nuclei towards periphery • Spherical
  38. 38. THE END By Nowera Zafar

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