Cajanus cajan L. belong to family fabaceae also known as
red gram, is an important world crop species widely
Cajanus cajan L. is an excellent source of protein, seeds
are using as vegetable flour additive or other food.
Its flower are self compatible and usually self pollinated.
Its forms root nodules associated with Rhizobium sp.
bacteria that function nitrogen fixation.
66 chromosome are present in Cajanus cajan L.
It is usually single stemmed, freely branching, and become
woody after a few months.
India is the world’s largest pigeonpea producer and grows
over 77% of the total world production.
Typical nutritional values for seeds are: moisture, 10.1
percent, protein 19.2 percent, fat, 1.5 percent,
carbohydrates, 57.3 percent, fiber 8.1 percent, and ash, 3.8
Present study was carried out with the following
1. To study of different explant for to
2. To study redifferentiation in Cajanus
Material & Methods
Healthy seeds of Cajanus cajan L. variety Balwan (NRS) used
in the present study were obtained from Mukesh seeds,
All the chemicals used in the present study were analytical
grade and were obtained from CDH, Himedia and Qualigens
India. Plant growth regulators, gelling agent, sucrose,
vitamins, colchicine etc, were obtained from “CDH fine
chemicals” and “Himedia” Mumbai.
The tissue culture laboratory was washed with effective
disinfectant (Teepol) followed by wiping with 2% sodium
hypochlorite solution or 95% ethyl alcohol, extran, Lysol,
zephiran or roccal.
MS medium used for present study:
30 g (3% w/v) sucrose.
Required quantities of plant growth regulators .
Micronutrient and Macronutrient were added.
8 g agar.
Make up final volume (i.e. 1000ml),
pH was adjusted between 5.4- 5.6.
Finally, the culture vessels were plugged, labeled and
autoclaved at 1210
C and 1.06 kg/cm2 for 20 min.
The present study, apical, axilliary buds, cotyledonary
leaves and hypocotyl parts were used as explants.
The present project report entitled “Tissue Culture Studies in
Cajanus cajan L.” presents findings and inferences of tissue
culture experiments carried out through different organ explants
in order to demonstrate a reproducible protocol for in vitro
regeneration in Cajanus cajan L.
Present study principally focuses on regeneration through
organogenesis and demonstrates organogenesis competence of
hypcotyl portions in Cajanus cajan L., chosen for the study.
Blümmel, M and Saxena, KB (2005): Genetic variation for forage
quality parameters in pigeonpea. Fourth International Food Legumes
Research Conference, New Delhi, October 18–22, 2005
Butler, JE (1910): The wilt disease of pigeonpea and the parasitism of
Neocosmospora vasinfecta Smith., India. Dept. Agr. Memoir. Bot. Ser
George, L; Eapen, SL (1994): Organogenesis and embryogenesis from
diverse explants in pigeonpea (Cajanus cajan L.). Plant Cell Rep., 13: