Reliable analysis of aflatoxin B1

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Fast, sensitive, accurate ELISA test kits for the quantitative detection of aflatoxin B1 contamination in food and feedstuffs. Two different formats are available to meet end-users requirements. For more information about rapid methods for aflatoxins and other mycotoxins visit www.tecnalab.com!

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Reliable analysis of aflatoxin B1

  1. 1. B ZERO AFLA B1 48 det. format 96 det. format MA221 MA220 48 det. format 96 det. format ELISA tools for the quantitative detection of aflatoxin B1 in cereals, feed, nuts, spices www.tecnalab.com MA222 MA224
  2. 2. PERFORMANCES  Quickness  High sensitivity, in compliance with worldwide requirements for food and feedstuffs  High accuracy  High reproducibility  Cost-effectivness without forgoing robustness, accuracy and precision  Development of an ELISA test kit format suitable for those implementing a few samples per session with no necessity of installing a lateral flow working station (no dedicated reader) Ease of use  ECONOMY www.tecnalab.com
  3. 3. B ZERO AFLA B1 • 15 minutes assays for the quantitative detection of aflatoxin B1 in cereals, high moisture maize, silage, mash, feed, nuts, dried fruits, DDGS, cottonseeds • Detection limit: 1 ppb aflatoxin B1 • Specificity of the assays: •Aflatoxin B1 100% •Aflatoxin B2 5% •Aflatoxin G1 19% •Aflatoxin G2 < 1% • www.tecnalab.com Sample preparation: grinding, extraction in methanol-water, filtration (EasyPur purification for cottonseeds) For silage and mash pH adjustment of the methanol extracts is advisable.
  4. 4. MAIZE Performances SPECIFICITY SENSITIVITY Cut Off < 1 ppb LOQ 1 ppb materials left over FAPAS proficiency tests PROFICIENCY TEST RESULTS Proficiency C1 (maize) Assigned AfB1 value (ppb) Mean result (ppb) T0497 3.9 (sat. range 2.2 – 5.6) 4.3 + 0.2 T0473 4.7 (sat. range 2.6 – 6.7) 5.8 + 0.8 5.2 (sat. range 2.3 – 7.5) 5.8 + 0.5 Assigned value (ppb) Celer AFLA B1 result (ppb) Z-Score AIA 2008 0.03 < 1.00 Satisfactory C2 (maize) AIA 2008 36.85 43.67 0.63 C3 (maize) AIA 2008 19.36 22.99 0.73 C4 (maize) AIA 2008 11.41 13.39 0.62 C1 (maize) AIA 2009 10.81 9.90 -0.30 C2 (maize) AIA 2009 36.97 37.29 0.02 C3 (maize) AIA 2009 20.78 20.18 -0.09 C4 (maize) AIA 2009 0.10 < 1.00 Satisfactory C1 (maize) AIA 2010 0.13 < 1.00 Satisfactory C2 (maize) AIA 2010 5.74 7.55 0.78 C3 (maize) AIA 2010 10.96 13.30 0.43 C4 (maize) www.tecnalab.com Sample cod. Code T04105 TRUENESS assessed on reference AIA 2010 24.38 24.61 0.02 MA1350-1 (maize) PTS 2013 1.59 1.27 -0.48 MA1350-2 (maize) PTS 2013 2.61 1.85 -0.75
  5. 5. FEED Performances Code materials left over FAPAS proficiency tests PRECISION Assigned AfB1 value (ppb) Mean result (ppb) T0491 11.3 (sat. range 6.4 – 16.3) 11.9 + 1.8 T04102 18.1 (sat. range 10.2 – 26.1) 15.4 + 0.9 T0486 TRUENESS assessed on reference 23.0 (sat. range 12.8 – 33.1) 24.8 + 2.5 Code Intra-assay CV (%), n = 9 Inter-assay CV (%) n = 3 T0491 5.2 12.1 T04102 2.8 3.9 T0486 6.1 2.0 Intra-assay CV was assessed by running 3 times the sampe sample in 3 sessions. Inter-assay CV was assessed by analysing the samples in 3 different days. www.tecnalab.com
  6. 6. NUTS & SPICES Performances SPECIFICITY (Cut Off) Hazelnuts < 1 ppb Pistachio nut < 1 ppb Raisins Figs SENSITIVITY (LOQ) < 1 ppb < 1 ppb Hazelnuts 1 ppb Pistachio nut 1 ppb Raisins 2 ppb Figs 2 ppb TRUENESS assessed on reference materials left over FAPAS proficiency tests Code Assigned AfB1 value (ppb) Mean result (ppb) T0484 Peanut 1.54 (sat. range 0.7 – 2.2) 1.4 + 0.9 T0483 www.tecnalab.com Type Fig 4.4 (sat. range 2.4 – 6.3) 4.4 + 0.0
  7. 7. Why a MASTER-CURVE version of Celer AFLA B1? Cost comparison in terms of wells when running 3 samples in a session COMMON ELISA KIT B ZERO TEST KIT Zero standard Zero standard Standard 1 SAMPLE 1 Standard 2 SAMPLE 2 Standard 3 SAMPLE 3 Total expence: 4 wells Standard 4 SAMPLE 1 SAMPLE 2 SAMPLE 3 Calculate the B/Bo of each sample and interpolate onto the provided virtual curves to find the concentration. Total expence: 8 wells RAFA 2013
  8. 8. B ZERO AFLA B1 has been compared in terms of performances on maize screening to Celer AFLA B1. (Effects of “master curve calibrations” on the performances of some ELISAs for food contaminants, Rosar et al, 2013, poster presentation @ Recent Advances In Food Analysis symposium, 4-9 Nov 2013, Prague, Czech Republic)
  9. 9. B ZERO MAIZE Performances AFLA B1 SPECIFICITY SENSITIVITY Cut Off < 1 ppb LOQ 2 ppb TRUENESS assessed on a Reference Material left over FAPAS proficiency tests Code Assigned AfB1 value (ppb) Mean result (ppb) T0497 3.9 (sat. range 2.2 – 5.6) 4.2 + 0.3 on a Trilogy Reference Material Code Assigned AfB1 value (ppb) Mean result (ppb) 18.8 16.5 + 0.6 Assigned AfB1 value (ppb) Mean result (ppb) MA1350-1 1.6 (sat. range 0.2 – 2.9) 1.5 + 0.2 MA1350-2 2.6 (sat. range 0.6 - 4.6) 2.0 + 0.2 TR_MTC9999D and on two Progetto Trieste Reference Materials www.tecnalab.com Code
  10. 10. B ZERO AFLA B1 ALL GOALS FULFILLED Quickness, easiness, high specificity and sensitivity were obtained. Trueness was high and proficiency tests z-scores were always satisfactory. www.tecnalab.com ALL GOALS FULFILLED Cost-effectiveness was obtained without forgoing robustness, accuracy and precision in a suitable format for costumers implementing a few samples per session.
  11. 11. More… Tecna’s port-folio of rapid and sensitive tools for mycotoxin screening in cereals B ZERO Available for aflatoxin B1, deoxynivalenol and zearalenone NEW! available for total aflatoxins, aflatoxin B1, fumonisins, zearalenone, deoxynivalenol, T2/HT2 toxins available for aflatoxins, ochratoxin A and fumonisins NEW! SMART STRIP NEW quantitative lateral flow for deoxynivalenol SOON AVAILABLE for aflatoxin B1 www.tecnalab.com

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