Fast, sensitive, accurate ELISA test kits for the quantitative detection of aflatoxin B1 contamination in food and feedstuffs. Two different formats are available to meet end-users requirements. For more information about rapid methods for aflatoxins and other mycotoxins visit www.tecnalab.com!

1. B ZERO
AFLA B1
48 det. format
96 det. format
MA221
MA220
48 det. format
96 det. format
ELISA tools for the quantitative detection of
aflatoxin B1 in cereals, feed, nuts, spices
www.tecnalab.com
MA222
MA224

2. PERFORMANCES

Quickness

High sensitivity, in compliance
with worldwide requirements
for food and feedstuffs

High accuracy

High reproducibility

Cost-effectivness
without
forgoing
robustness,
accuracy and precision

Development of an ELISA test
kit format suitable for those
implementing a few samples
per session with no necessity
of installing a lateral flow
working
station
(no
dedicated reader)
Ease of use

ECONOMY
www.tecnalab.com

3. B ZERO
AFLA B1
•
15 minutes assays for the quantitative detection of aflatoxin B1 in
cereals, high moisture maize, silage, mash, feed, nuts, dried fruits, DDGS,
cottonseeds
•
Detection limit: 1 ppb aflatoxin B1
•
Specificity of the assays:
•Aflatoxin B1 100%
•Aflatoxin B2 5%
•Aflatoxin G1 19%
•Aflatoxin G2 < 1%
•
www.tecnalab.com
Sample preparation: grinding, extraction in methanol-water, filtration
(EasyPur purification for cottonseeds) For silage and mash pH
adjustment of the methanol extracts is advisable.

4. MAIZE Performances
SPECIFICITY
SENSITIVITY
Cut Off < 1 ppb
LOQ 1 ppb
materials left over FAPAS proficiency tests
PROFICIENCY
TEST RESULTS
Proficiency
C1 (maize)
Assigned AfB1 value (ppb)
Mean result (ppb)
T0497
3.9 (sat. range 2.2 – 5.6)
4.3 + 0.2
T0473
4.7 (sat. range 2.6 – 6.7)
5.8 + 0.8
5.2 (sat. range 2.3 – 7.5)
5.8 + 0.5
Assigned value (ppb)
Celer AFLA B1 result (ppb)
Z-Score
AIA 2008
0.03
< 1.00
Satisfactory
C2 (maize)
AIA 2008
36.85
43.67
0.63
C3 (maize)
AIA 2008
19.36
22.99
0.73
C4 (maize)
AIA 2008
11.41
13.39
0.62
C1 (maize)
AIA 2009
10.81
9.90
-0.30
C2 (maize)
AIA 2009
36.97
37.29
0.02
C3 (maize)
AIA 2009
20.78
20.18
-0.09
C4 (maize)
AIA 2009
0.10
< 1.00
Satisfactory
C1 (maize)
AIA 2010
0.13
< 1.00
Satisfactory
C2 (maize)
AIA 2010
5.74
7.55
0.78
C3 (maize)
AIA 2010
10.96
13.30
0.43
C4 (maize)
www.tecnalab.com
Sample cod.
Code
T04105
TRUENESS assessed on reference
AIA 2010
24.38
24.61
0.02
MA1350-1 (maize)
PTS 2013
1.59
1.27
-0.48
MA1350-2 (maize)
PTS 2013
2.61
1.85
-0.75

5. FEED Performances
Code
materials left over FAPAS proficiency tests
PRECISION
Assigned AfB1 value (ppb)
Mean result (ppb)
T0491
11.3 (sat. range 6.4 – 16.3)
11.9 + 1.8
T04102
18.1 (sat. range 10.2 – 26.1)
15.4 + 0.9
T0486
TRUENESS assessed on reference
23.0 (sat. range 12.8 – 33.1)
24.8 + 2.5
Code
Intra-assay CV (%), n = 9
Inter-assay CV (%) n = 3
T0491
5.2
12.1
T04102
2.8
3.9
T0486
6.1
2.0
Intra-assay CV was assessed by running 3 times the sampe sample in 3 sessions.
Inter-assay CV was assessed by analysing the samples in 3 different days.
www.tecnalab.com

6. NUTS & SPICES Performances
SPECIFICITY (Cut Off)
Hazelnuts
< 1 ppb
Pistachio nut < 1 ppb
Raisins
Figs
SENSITIVITY (LOQ)
< 1 ppb
< 1 ppb
Hazelnuts
1 ppb
Pistachio nut 1 ppb
Raisins
2 ppb
Figs
2 ppb
TRUENESS assessed on reference
materials left over FAPAS proficiency tests
Code
Assigned AfB1 value (ppb)
Mean result (ppb)
T0484
Peanut
1.54 (sat. range 0.7 – 2.2)
1.4 + 0.9
T0483
www.tecnalab.com
Type
Fig
4.4 (sat. range 2.4 – 6.3)
4.4 + 0.0

7. Why a MASTER-CURVE version of Celer AFLA B1?
Cost comparison in terms of wells when running 3 samples in a session
COMMON ELISA KIT
B ZERO TEST KIT
Zero standard
Zero standard
Standard 1
SAMPLE 1
Standard 2
SAMPLE 2
Standard 3
SAMPLE 3
Total expence: 4 wells
Standard 4
SAMPLE 1
SAMPLE 2
SAMPLE 3
Calculate the B/Bo of each
sample and interpolate
onto the provided virtual
curves to find the
concentration.
Total expence: 8 wells
RAFA 2013

8. B ZERO
AFLA B1
has been compared in terms of
performances on maize screening
to Celer AFLA B1.
(Effects of “master curve calibrations”
on the performances of some ELISAs for
food contaminants, Rosar et al, 2013,
poster presentation @ Recent
Advances In Food Analysis symposium,
4-9 Nov 2013, Prague, Czech Republic)

9. B ZERO
MAIZE Performances
AFLA B1
SPECIFICITY
SENSITIVITY
Cut Off < 1 ppb
LOQ 2 ppb
TRUENESS
assessed on a Reference
Material left over FAPAS proficiency tests
Code
Assigned AfB1 value (ppb)
Mean result (ppb)
T0497
3.9 (sat. range 2.2 – 5.6)
4.2 + 0.3
on a Trilogy Reference Material
Code
Assigned AfB1 value (ppb)
Mean result (ppb)
18.8
16.5 + 0.6
Assigned AfB1 value (ppb)
Mean result (ppb)
MA1350-1
1.6 (sat. range 0.2 – 2.9)
1.5 + 0.2
MA1350-2
2.6 (sat. range 0.6 - 4.6)
2.0 + 0.2
TR_MTC9999D
and on two Progetto Trieste Reference
Materials
www.tecnalab.com
Code

10. B ZERO
AFLA B1
ALL GOALS FULFILLED
Quickness, easiness, high
specificity and sensitivity were
obtained.
Trueness was high and
proficiency tests z-scores were
always satisfactory.
www.tecnalab.com
ALL GOALS FULFILLED
Cost-effectiveness was
obtained without forgoing
robustness, accuracy and
precision in a suitable format for
costumers implementing a few
samples per session.

11. More…
Tecna’s port-folio of rapid and sensitive tools for mycotoxin
screening in cereals
B ZERO
Available for aflatoxin B1, deoxynivalenol and zearalenone NEW!
available for total aflatoxins, aflatoxin B1, fumonisins,
zearalenone, deoxynivalenol, T2/HT2 toxins
available for aflatoxins, ochratoxin A and fumonisins NEW!
SMART STRIP
NEW quantitative lateral flow for deoxynivalenol
SOON AVAILABLE for aflatoxin B1
www.tecnalab.com