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Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
Identification of Biological Molecules
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Identification of Biological Molecules

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  • 1. Identification of Biological Molecules ALBIO9700/2006JK
  • 2. Testing for the presence of sugars (Benedict’s test) ALBIO9700/2006JK
  • 3. Testing for the presence of sugars (Benedict’s test) ALBIO9700/2006JK
  • 4. Testing for the presence of sugars ALBIO9700/2006JK
  • 5. Testing for the presence of starch (iodine solution) ALBIO9700/2006JK
  • 6. Testing for the presence of lipids (emulsion test) ALBIO9700/2006JK
  • 7. Sudan III• Tissue that has been ground up is transferred to a test tube containing water and boiled• Oil droplets will escape the tissue and rise to the surface• Add 2 cm3 Sudan III, shake and allow the oil to settle• Oil will be stained red ALBIO9700/2006JK
  • 8. Testing for presence of protein ALBIO9700/2006JK
  • 9. Biuret test• Suitable for soluble proteins• Add equal amount of 1% potassium hydroxide (KOH) to solution• Add a few drops of 1% copper (II) sulphate (CuSO4) solution down the side of test tube• A blue ring at the surface of the solution indicates protein• On shaking, the blue ring disappears and the solution turns purple ALBIO9700/2006JK
  • 10. Millon’s test• For insoluble proteins• 6 drops of Millon’s reagent to a small amount (3 cm3) of solution or suspension of protein and boil for 5 minutes• After cooling, add a few drops of 1% sodium nitrite• If protein is present, a brick-red precipitate appears on the surface ALBIO9700/2006JK

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