Identification of Biological Molecules

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Identification of Biological Molecules

  1. 1. Identification of Biological Molecules ALBIO9700/2006JK
  2. 2. Testing for the presence of sugars (Benedict’s test) ALBIO9700/2006JK
  3. 3. Testing for the presence of sugars (Benedict’s test) ALBIO9700/2006JK
  4. 4. Testing for the presence of sugars ALBIO9700/2006JK
  5. 5. Testing for the presence of starch (iodine solution) ALBIO9700/2006JK
  6. 6. Testing for the presence of lipids (emulsion test) ALBIO9700/2006JK
  7. 7. Sudan III• Tissue that has been ground up is transferred to a test tube containing water and boiled• Oil droplets will escape the tissue and rise to the surface• Add 2 cm3 Sudan III, shake and allow the oil to settle• Oil will be stained red ALBIO9700/2006JK
  8. 8. Testing for presence of protein ALBIO9700/2006JK
  9. 9. Biuret test• Suitable for soluble proteins• Add equal amount of 1% potassium hydroxide (KOH) to solution• Add a few drops of 1% copper (II) sulphate (CuSO4) solution down the side of test tube• A blue ring at the surface of the solution indicates protein• On shaking, the blue ring disappears and the solution turns purple ALBIO9700/2006JK
  10. 10. Millon’s test• For insoluble proteins• 6 drops of Millon’s reagent to a small amount (3 cm3) of solution or suspension of protein and boil for 5 minutes• After cooling, add a few drops of 1% sodium nitrite• If protein is present, a brick-red precipitate appears on the surface ALBIO9700/2006JK

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