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Rotavirus Review

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MLS Students At Armstrong present on Rotovirus

MLS Students At Armstrong present on Rotovirus

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  • 1. By:Denisse Class &Nirzari Amin
  • 2.  Rotavirus is a non-enveloped virus that comes from a family called Reoviridai. Derives the name from the Latin Rota which means wheel. It appears wheel like when looked under an electric microscope. Very stable and may remain viable for weeks or months if not disinfected Once cured, it can reoccur at any age. But the subsequent infections are usually less severe
  • 3.  Can cause:  Gastroenteritis  Dehydration
  • 4.  This virus can easily be spread through children before and/or after becoming sick with diarrhea. Usually transmitted by fecal-oral route  Virus must be shed of an infected person and somehow entered into a susceptible person to cause infection. Also caused by contaminated hands, water, and, objects.
  • 5.  It has been observed that improved sanitation does not decrease the infection transmission. Due to this, the primary treatment is vaccination.  Few current examples include; RotaTeq, Rotatrix No antiviral drug has yet been discovered. Treatment is nonspecific but usually treated for dehydration.
  • 6.  Symptoms usually appear within 12 to 48 hrs. after exposure and last till 1-3 days.  Watery diarrhea  Vomiting  Fever  Headache  Abdominal pain  Chills
  • 7.  Identification of etiologic agent Based on symptoms and physical exam Elisa test Latex agglutination (for rapid results)
  • 8.  Elisa PCR
  • 9.  This is an in-vitro test for the  Limitations: determination of rotavirus  Stool sample varies by patient antigen in feces.  Patients with infection for a long period of time results will not Polyclonal anti-rotavirus and show as an active infection. anti-rotavirus monoclonal  Early treatment with proper antibodies are used. antibiotics. Specificity – 97% Sensitivity – 100% Expectations:  Healthy individuals should test negative.  Presence of rotavirus antigen should be present .
  • 10.  Break the wells need it for samples plus two controls. Prepare controls. Add 100 µL of specimen, incubate for 30 min. Add 2 drops of reagent 1 Blue sol. to each well, incubate for 5 min. then wash Add 2 drops of reagent 2 red sol. incubate for 5 min and then wash. Add 2 drops of chromogen sol. @room temp. for 5 min. DO NOT WASH. Add 2 drops of stop sol. Mix then read results .
  • 11.  Limitations:  Expected results:  For the making of the  Amplification/Extension new template the of DNA. primers have to be very  Allows scientists to specific and particular to sequence nucleotides. the template. These nucleotides are  The primers must be very helpful in studying only capable of binding the molecular with the template and epidemiology of not each other because Rotavirus. this can cause the amplification of a short, useless DNA.
  • 12. ELISA PCR 97% specificity  Less specific 100% sensitivity  Super sensitive Fluid sample  Fluid sample Results in 45-60 min  2 to 3 hours
  • 13.  www.rapidtest.com/pdf/Rotavirus_8306-3.pdf http://www.hopkinsmedicine.org/heic/ID/rotavirus/ http://www.microbac.com/services/pcr_process.php Brasier,Alan R., Adolfo Garcia-Sastre, A, Lemon, S. M., & ebrary, I. (2009). Cellular signaling and innate immune responses to RNA virus infections. Washington, D.C. : ASM Press. Wark, P. (2008). Paediatric Respiratory Reviews. Mini-symposium: Innate immunity in the lung. Paediatric Respiratory Reviews, 9(4), 233-235. Retrieved from EBSCOhost.
  • 14.  Rotavirus. In http://www.cdc.gov. Retrieved 09/26/2011, from http://www.cdc.gov/rotavirus/index.html. Scott M, Janneck L, Dent R, Merino D. Rotavirus. Retrieved from http://www.brown.edu/Courses/Bio_160/Projects2004/rotavirus/ Pathology.htm http://faculty.plattsburgh.edu/donald.slish/PCR.html http://www.humenhealth.com/rotavirus-infection/rotavirus- infection.asp http://www.humenhealth.com/rotavirus-2