For over 25 years, ALCAT has been the pioneer in developing kits used for testing of gluten allergy. A gluten allergy kit from ALCAT is capable of determining the extent of allergy that a patient has to the 350 plus chemicals that cause gluten sensitivity.

1. GUT HEALTH AND SYSTEMIC DISEASE
Your gut holds the keys to optimal health
Keith R. Holden, M.D.

2. FOOD SENSITIVITY & SYSTEMIC COMPLAINTS

3. GUT INFLAMMATION & SYSTEMIC DISEASE
 Total surface area of GI tract > 200X surface area
of skin
 GI tract is the largest surface area where we
interact with our environment
 Gut immune dysregulation + excessive
inflammation = systemic disease

4. GUT FUNCTION & AUTOIMMUNE DISEASE
 Environmental triggers (food, microbes)
 Genetic tendency for immune system to overreact
to triggers
 Intestinal hyperpermeability associated with many
diseases – CD, T1DM, MS, RA, IBD

5. CELIAC DISEASE – CLUES FOR AUTOIMMUNITY
 TRIAD
 1) Environmental trigger (antigen, e.g. gluten)
 2) Genetic susceptibility (HLA pattern particularly
efficient at presenting antigen (gluten) to immune cells
(HLA-DQ2/HLA-DQ8)
 3) Intestinal hyperpermeability AKA “leaky gut” – tight
junctions moderated by specific proteins
Fasano A. Sci Am. August 2009;301(2):54-61

6. ALESSIO FASANO, M.D.
Prof. of Pediatrics & Dir. of Ctr. for Celiac Disease
Research at Univ. of Maryland School of Med.
 “Together with the gut-associated lymphoid tissue
and the neuro-endocrine network, the intestinal
barrier, with its tight junctions, controls the
equilibrium between tolerance and immunity to
nonself-antigens. When the finely tuned trafficking
of macromolecules is dysregulated in genetically
susceptible individuals, both intestinal and
extraintestinal autoimmune disorders can occur.”

7. HELICOBACTER PYLORI
 Most common chronic bacterial inf. in the world
 Present in half of world’s population
 Affinity for stomach mucosa
 Disrupts permeability of GI tract
 Induces a sustained immune response (excess
inflammation)
 May progress from gastritis to malignancy
 Molecular mimicry results in systemic AD

8. MOLECULAR MIMICRY (MISTAKEN IDENTITY)
 Environmental exposure to antigens (food,
microbes)
 In genetically susceptible individuals induces cross-
reaction with structurally similar AA motifs in tissue
 CD patients have ~ 10X rate of autoimmune thyroid
disease as nonceliacs reflective of gluten/gliadin
Ag-Ab complexes for thyroid tissue

9. H. PYLORI & SYSTEMIC DISEASE
 IHD
 CVD
 ITP
 Iron-deficiency anemia
 Idiopathic anterior uveitis
 Autoimmune thyroid disease (Graves, Hashimotos)
 MALT lymphoma

10. BACTERIAL ASSOC. WITH SYSTEMIC DISEASE
 Klebsiella penumoniae – spondyloarthritis (AS)
 Yersinia enterocolitica – autoimmune thyroid dis.
 Campylobacter jejuni – reactive arthritis, GBS
 Salmonella – reactive arthritis
 E. coli – HUS
 Candida albicans – atopic dermatitis, CD
 Strep pyogenes – Rheumatic Fever
 Chlamydia – MS
 Citrobacter, Klebsiella, Proteus - RA

11. SUMMARY
 Studies are showing that the triad of
(1)environmental triggers (food, microbes),
(2)genetic susceptibility, and (3)intestinal
hyperpermeability play a key role in the
development of autoimmune and other systemic
diseases through uncontrolled inflammation.
 Other issues: digestion and proper assimilation of
micronutrients necessary for optimal immune
status; beneficial bacteria’s role in digestion and
immunity; and the gut-brain axis

12. GUT-BRAIN AXIS

13. MY APPROACH
 Comprehensive History & Physical
 Antecedents and Triggers
 Detoxification Questionnaires
 Toxin exposure history (past and present)
 Signs and symptoms of micronutrient deficiencies
 Signs and symptoms of “leaky gut” & dysbiosis
 Review of bowel habits in detail
 Detailed physical exam

14. DETAILED ORODENTAL ASSESSMENT
 Plaque, amalgams, crowns, bridges
(malocclusion?)
 Mixed metals (galvanism)
 Old root canals (notorious for infection)
 TMJ (CST, chiropractor)
 Tongue, gums, mucous membranes, tonsils

15. MEDICATION REVIEW
 Culprits for “leaky gut,” micronutrient deficiencies,
and microbial overgrowth:
 PPI, H2 blockers, antacids
 NSAIDS
 Corticosteroids
 Statins (CoQ10)
 Antibiotics

16. NUTRITIONAL ASSESSMENT
 Dietary review
 Standard American Diet (SAD)
 FM Comprehensive Elimination Diet – stress
elimination of wheat/gluten, dairy, sugar, high-
glycemic index foods (grains), caffeine, ETOH

17. ALCAT
 Measures the change in WBCs in size and number after
antigen exposure
 Screens for reactivity to foods, additives, colorings,
drugs, and molds

18. ALCAT AND WEIGHT LOSS
 “Even when one focuses only on the changes
occurred in the experimental group without
comparison to the control group, the data reveal
that 98 percent of the subjects following the ALCAT
plan either lost scale weight or improved body
composition.”
Kaats GR, Pullin DP, Parker KL. The short term efficacy of the
ALCAT test of food sensitivities to facilitate changes in body
composition and self-reported disease symptoms: a
randomized controlled study. The Bariatrician. Spring 1996.

19. PRIORITY TESTING – CDSA/PARASITOLOGY
 CDSA with microbial stool analysis including
parasitology and H. pylori screening
 Assesses for dysbiosis – imbalance of beneficial to
potentially pathogenic microbes
 Some assess for inflammatory markers, bile salts,
HCL status, and pancreatic enzyme function
 Does not assess for SIBO

20. 5 RS OF FUNCTIONAL MEDICINE
 Remove
 Replace
 Repair
 Reinoculate
 Rebalance

21. REMOVE (STRESSORS)
 Biomechanical imbalance/subluxation
 Address stress including old emotional toxins
 Food sensitivities (FM CED, ALCAT)
 Detoxify xenobiotics (ext. toxins) and endotoxins
 Pathogenic microbes (bacteria, yeast, parasites)

22. REPLACE
 HCL
 Digestive enzymes
 Bile salts
 Fiber

23. REPAIR
 Rest
 Vitamins (pantothenate, E, A, D, C)
 Activated B vitamins (pyridoxal 5 phosphate, folinic
acid/L-methylene tetrahydrofolate,
methylcobalamin/adenosycobalamin)
 Minerals (zinc)
 Support of GALT fxn (lactoferrin, whey, Ig)
 Antioxidants (catechins)
 Anti-inflammatories (curcumin, EPA/DHA/GLA/oleic
acid)
 Glutamine and aloe vera

24. REINOCULATE (BENEFICIAL FLORA)
 Probiotics (Bifidobacter, Lactobacillus, S. boulardii)
 Prebiotics (Inulin, FOS, Arabinogalactan)

25. REBALANCE
 Lifestyle changes
 Scheduling rest and relaxation; adequate sleep
 Mindful eating
 Balance autonomic nervous system
 Balance brain waves

26. BASIC NUTRITIONAL SUPPLEMENTS
 High potency MVI/MM w/aBv – Prothera MultiThera
 Activated B vitamins – NeuroBiologix Neuro-Immune
Stabilizer (transdermal)
 Essential Fatty Acids blend (EPA/DHA 3000mg) –
Natura Health Products Beyond Essential Fats
 Probiotics 25-900 bil cfu (Ther-Biotic Complete, VSL#3)
 Vitamin D (based on blood levels)
 Medical Food (Xymogen I5)

27. REMOVE
 DFH GI MicrobX or Prothera Undecylex
 Parasites: BioPure Artemesinin or Mimosa Pudica
 Bacterial: Syntrion SyGest + SanPharma Firmus
 Fungal: Syntrion SyRegule + SanPharma Candida-
Roqueforti suppositories
 Fungal Sinus: Syntrion SyFungin & Argentyn 23 NS
 PEKANA Mucan, Helmin, Opsonot, Mundipur
 Natural Immunogenics Argentyn 23 liquid/spray
 Detoxification: PEKANA Basic Detoxification &
Drainage Kit (apo-Hepat, Renelix, Itires)
 Syntrion SyDetox for “hot liver” signs & symptoms

28. TREATING H. PYLORI WITHOUT ANTIBIOTICS
 OrthoMolecular Products Pyloricil (Zinc carnosine,
Mastic gum, Berberine, Bismuth citrate) x 2 mos
 Lactoferrin
 Probiotics/Kefir
 Always check H. pylori stool Ag 1 month after
completion of therapy

29. PARASITE THERAPY
 B. hominis: SMX/TMP + Metronidazole/Secnidazole
 Cryptosporidia: Nitazoxanide (Alinia)
 Dientamoeba fragiis: Iodoquinol
 Enterobius Vermicularis (PW): Pyrantel Pamoate
 Giardia: Tinidazole/Metronidazole
 Entamoeba histolytica: Iodoquinol
 Worms: Albendazole/Mebendazole
 Apothecure pharmacy – Paragone 5 and Paragone 6
(compounded combination of antiparasitics)

30. REPLACE
 Theramedix DGX
 Betaine HCL
 Standard Process Cholachol
 Natural Factors PGX (konjac root)

31. REPAIR
 GI Cocktail: 1 heaping tsp of glutamine powder in ½
cup of George’s Aloe Vera juice 2-3 x day
 ECGC (green tea)
 Curcumin
 Xymogen IgG 2000/Xymogen I5

32. REINOCULATE
 Klaire Labs Ther-Biotic Complete (25 bil CFU)
 VSL#3 (caps 112.5 bil CFU, packets 450 bil CFU,
and DS packets (requires Rx) 900 bil CFU
 Saccharomyces boulardii (C. difficile)
 Thorne Research Arabinex

33. REBALANCE
 Address emotional toxins - ERT, counseling,
journaling, Inner Child meditation, compassionate
forgiveness
 Balance autonomic nervous system - acupuncture,
meditation, breath work, yoga, Tai Chi, Ballroom
Dancing, PEMF, Microcurrent therapy (CES), Low-
level laser

34. SPECIALIST REFERRAL LIST
 Biological/Holistic Dentist
 Chiropractor
 Acupuncturist
 Massage Therapist
 Craniosacral Therapist
 Eden Energy Practitioner
 Holistic Psychological Counselors

35. FINAL ADVICE
 Stay open to possibilities
 See the world through the eyes of a child
 Embrace change
 Take with you what resonates with you and leave
the rest alone (what feels right)
 Never stop evolving (transformation/growth)

36. MINDFULNESS
“Mindfulness practice means that we commit fully in
each moment to be present; inviting ourselves to
interface with this moment in full awareness, with
the intention to embody as best we can an
orientation of calmness, mindfulness, and
equanimity right here and right now.”
Jon Kabat-Zinn, Wherever You Go, There You Are

37. ALCAT TEST
Cell Science Systems
852 South Military Trail
Deerfield Beach, Florida 33442
www.alcat.com